[Objectives]To isolate and analyze alkaloids in Fructus Hordei Germinatus.[Methods]The alkaloids in the Fructus Hordei Germinatus were extracted by ultrasonic technology,and analyzed by the ultra-performance liquid ch...[Objectives]To isolate and analyze alkaloids in Fructus Hordei Germinatus.[Methods]The alkaloids in the Fructus Hordei Germinatus were extracted by ultrasonic technology,and analyzed by the ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS) technique.[Results] According to the mass spectrometry information,compared with the literature reports,the possible structures of seven alkaloids in the Fructus Hordei Germinatus could be inferred.Compounds 1,2 and 3 were organic amine alkaloids,respectively tyramine and N-methyltyramine,hordenine,compounds 4,5,6 and 7 were indole alkaloids,respectively,tryptamine,gramine,harmine,and bufotenidine.The alkaloids in the Fructus Hordei Germinatus were separated by preparative high performance liquid chromatography(p HPLC) technique,a compound was obtained and identified by UPLC-MS/MS and nuclear magnetic resonance(NMR) technique as hordenine.[Conclusions]This experiment is expected to lay a foundation for the development of alkaloids in the Fructus Hordei Germinatus.展开更多
Objective:This study aimed to determine the free and hidden fumonisins in Hordei Fructus Germinatus samples and to investigate the transfer rates of these mycotoxins from Hordei Fructus Germinatus to its decoction.Mat...Objective:This study aimed to determine the free and hidden fumonisins in Hordei Fructus Germinatus samples and to investigate the transfer rates of these mycotoxins from Hordei Fructus Germinatus to its decoction.Materials and Methods:The contamination levels of free and hidden fumonisins in a total of 60 Hordei Fructus Germinatus samples were analyzed using high-performance liquid chromatography coupled to tandem mass spectrometry(LC-MS/MS).The decoction procedure was simulated with a highly contaminated Hordei Fructus Germinatus sample,and fumonisins in the resulting decoction preparation were determined by LC-MS/MS.Results:Among all the samples,8.3%were contaminated with free fumonisins(FB1 and/or FB2)and 13.3%were contaminated with total fumonisins(free+hidden,measured as hydrolyzed fumonisins,i.e.,HFB1 and/or HFB2).The concentrations of FB1 and HFB1 reached up to 83 and 95μg/kg,respectively,whereas FB2 and HFB2 were detected only in traces.The transfer rates of free and total fumonisins from Hordei Fructus Germinatus to the decoction were 71.8%and 83.3%for FB1 and FB2,respectively.In comparison,much lower transfer rates were found for total fumonisins,i.e.,38.2%and 24.7%for HFB1 and HFB2,respectively.Conclusion:The incidence and contamination levels of free and hidden fumonisins in Hordei Fructus Germinatus samples were generally low.Regarding decoction preparation,the transfer rates of free fumonisins into the decoction were high,whereas a large part of hidden fumonisins were retained in Hordei Fructus Germinatus rather than migrating into water.展开更多
基金Supported by Innovating Research Program of Postgraduates of Southwest Minzu University in 2017(CX2017SZ086)
文摘[Objectives]To isolate and analyze alkaloids in Fructus Hordei Germinatus.[Methods]The alkaloids in the Fructus Hordei Germinatus were extracted by ultrasonic technology,and analyzed by the ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS) technique.[Results] According to the mass spectrometry information,compared with the literature reports,the possible structures of seven alkaloids in the Fructus Hordei Germinatus could be inferred.Compounds 1,2 and 3 were organic amine alkaloids,respectively tyramine and N-methyltyramine,hordenine,compounds 4,5,6 and 7 were indole alkaloids,respectively,tryptamine,gramine,harmine,and bufotenidine.The alkaloids in the Fructus Hordei Germinatus were separated by preparative high performance liquid chromatography(p HPLC) technique,a compound was obtained and identified by UPLC-MS/MS and nuclear magnetic resonance(NMR) technique as hordenine.[Conclusions]This experiment is expected to lay a foundation for the development of alkaloids in the Fructus Hordei Germinatus.
基金the National Natural Science Foundation of China (No. 81891014,81603241)key project at central government level The ability establishment of sustainable use for valuable Chinese medicine resources (No. 2060302)+1 种基金the National Key Research and Development Program 250 of China (No. 2017YFC1700701)the Research Program for Basic Public Welfare of Zhejiang Province (No. LGN18H280003)。
文摘Objective:This study aimed to determine the free and hidden fumonisins in Hordei Fructus Germinatus samples and to investigate the transfer rates of these mycotoxins from Hordei Fructus Germinatus to its decoction.Materials and Methods:The contamination levels of free and hidden fumonisins in a total of 60 Hordei Fructus Germinatus samples were analyzed using high-performance liquid chromatography coupled to tandem mass spectrometry(LC-MS/MS).The decoction procedure was simulated with a highly contaminated Hordei Fructus Germinatus sample,and fumonisins in the resulting decoction preparation were determined by LC-MS/MS.Results:Among all the samples,8.3%were contaminated with free fumonisins(FB1 and/or FB2)and 13.3%were contaminated with total fumonisins(free+hidden,measured as hydrolyzed fumonisins,i.e.,HFB1 and/or HFB2).The concentrations of FB1 and HFB1 reached up to 83 and 95μg/kg,respectively,whereas FB2 and HFB2 were detected only in traces.The transfer rates of free and total fumonisins from Hordei Fructus Germinatus to the decoction were 71.8%and 83.3%for FB1 and FB2,respectively.In comparison,much lower transfer rates were found for total fumonisins,i.e.,38.2%and 24.7%for HFB1 and HFB2,respectively.Conclusion:The incidence and contamination levels of free and hidden fumonisins in Hordei Fructus Germinatus samples were generally low.Regarding decoction preparation,the transfer rates of free fumonisins into the decoction were high,whereas a large part of hidden fumonisins were retained in Hordei Fructus Germinatus rather than migrating into water.
