Strontium has similar chemical properties to calcium and has recently been recognized as a non-essential beneficial element for plants.In order to compare the effects of strontium and calcium on improving salt toleran...Strontium has similar chemical properties to calcium and has recently been recognized as a non-essential beneficial element for plants.In order to compare the effects of strontium and calcium on improving salt tolerance of Chinese cabbage during the germination stage,2,4,and 8 mmol/L of SrCl_(2),CaCl_(2) or an equimolar mixture of both were added separately to a 150 mmol/L NaCl solution.The results showed that Ca-Sr addition significantly increased seed viability,seed vigor,seed germination rate and seed germination uniformity of Chinese cabbage compared with the salt-control group.The differences in germination percentage(GP)and germination energy(GE)among the Ca-addition and Sr-addition groups were not significant,and the differences in coefficient of rate of germination(CRG),index of rate of germination(IRG)and coefficient of variation of the germination time(CVT)were relatively small,but clear differences were observed in germination index(GI),vigor index(VI)and coefficient of uniformity of germination(CUG).The results of GI and VI indicated that the higher the concentration of Ca-addition or Sr-addition,the more significant the enhancement of seed vigor.Under saline stress(150 mmol/L NaCl),the Ca-Sr co-addition outperformed Sr-treatment alone,and Ca-addition achieved the highest seed vigor at equivalent concentrations.Furthermore,all Ca-Sr treatments significantly enhanced the uniformity of Chinese cabbage sprouts exposed to 150 mM NaCl,with the best uniformity improved by the addition of 2 and 4 mmol/L SrCl_(2).Ca-Sr treatments increased the salt tolerance of Chinese cabbage sprouts during the germination stage mainly because the Ca2+and Sr2+significantly enhanced plasma membrane stability and reduced oxidative stress(as indicated by decreased contents of malondialdehyde and O2⋅-contents)in sprouts.The decrease of soluble sugar and proline content caused by Ca-Sr addition implies that elevated levels of these osmolytes were not the primary contributors to improved seed germinability in Chinese cabbage.These findings demonstrate that Sr is a beneficial element for enhancing salt tolerance in plants,laying a theoretical foundation for the development and application of strontium in agriculture.展开更多
[Objective] This study aimed to investigate the effects of salt stress on seed germination and seedling growth in radish（Raphanus sativus L.）.[Method] First,effects of soaking seeds with clear water and different co...[Objective] This study aimed to investigate the effects of salt stress on seed germination and seedling growth in radish（Raphanus sativus L.）.[Method] First,effects of soaking seeds with clear water and different concentrations of saline solution were compared; then,different concentrations of salt solution （0.2％-1.0％） was used to treat radish during the whole germination period.[Result] soaking seeds with clear water can improve the germination indicators of radish seeds exposed to high salt stress; low salt concentration（0.2％,0.4％） had little effect on the growth indicators of radish seedlings,and 0.2％ salt concentration can even promote the growth of radish seedlings,however,the high salinity had a bad inhibition on seedling growth,the higher the salt concentration,the stronger the inhibition.[Conclusion] According to the comprehensive comparison,the order of the six varieties in salt-tolerance was as follows：Xinxiayuanbai＞W1029,Xiayuanbai ＞ Nanyangbailizhi,Chunyidianhong＞ Sijihong 3.展开更多
Drought is considered one of the leading abiotic constraints to agricultural crop production globally.Present study was conducted to assess the effects of different drought treatments(viz.Control,10%PEG,and 20%PEG)on ...Drought is considered one of the leading abiotic constraints to agricultural crop production globally.Present study was conducted to assess the effects of different drought treatments(viz.Control,10%PEG,and 20%PEG)on seed germination,germination indices,seedling traits,and drought tolerance indices of sesame.Our results showed that maximum reduction in the studied parameters was observed at higher PEG concentration(i.e.,20%PEG).As compared to control,the drought treatments viz.10%and 20%PEG decreased the values for germination indices,such as germination percentage,coefficient of variation of germination time,germination index,and seedling vigor index.Similarly,for seedling traits,the values were decreased for root length,shoot length,root shoot ratio,root fresh weight,shoot fresh weight,root dry weight and shoot dry weight under 10%and 20%PEG treatments significantly in comparison with control.Furthermore,relative to control,the values for drought tolerance indices,such as germination drought tolerance index,root length drought tolerance index,shoot length drought tolerance index,total seedling length drought tolerance index,root fresh weight drought tolerance index,shoot fresh weight drought tolerance index,total fresh weight drought tolerance index,root dry weight drought tolerance index,shoot dry weight drought tolerance index and total dry weight drought tolerance index were also reduced under 10%and 20%PEG treatments,respectively.Our results confirms that drought impact on seed germination and seedling traits could be quantified by using different indices which can further help to design drought adaptation and mitigation strategies.Based on these results it can be concluded that germination indices,seedling traits,and drought tolerance indices have great potential to simulate drought stress impacts on different crop traits thus they should be used in all kinds of stress related studies.展开更多
Chilling is one of the major abiotic stresses for plants,especially for rice cultivation.Many essential metabolic processes for growth and development are temperature-dependent.In that case,reducing the negative effec...Chilling is one of the major abiotic stresses for plants,especially for rice cultivation.Many essential metabolic processes for growth and development are temperature-dependent.In that case,reducing the negative effects of cold stress using exogenous chemicals is a possible option.Therefore,the current study examined the effects of pre-sowing seed treatment with different chemicals,viz.hydrogen peroxide(H_(2)O_(2)),salicylic acid(SA),calcium chloride(CaCl_(2)),thiourea(TU),and citric acid(CA)on the germination of rice seeds(cv.BRRI dhan28)under chilling environments.Rice seeds were soaked in distilled water(control),10 mM CA,2 mM SA,10 mM CaCl_(2),10 mM H_(2)O_(2),and 10 mM TU solutions for 24 h.After that,seeds were exposed to chilling stress by incubating at 4±1℃ for 8 h,followed by at 25±2℃ for 16 h for 7 days.Exposure to chilling stress significantly reduced thefinal germination percent(13.6%),germination rate index(36.0%),coefficient of the velocity(25.0%),shoot fresh weight(44.4%),and root fresh weight(60.5%).Moreover,chilling induced oxidative damage and reduced the activity of antioxidant enzymes(catalase and ascorbate peroxidase).In contrast,treatments with H_(2)O_(2),SA,CaCl_(2),TU,and CA considerably enhanced germination indices and seedling growth compared to chilling stress condi-tions.The study showed that priming with H_(2)O_(2),SA,CaCl_(2),TU,and CA significantly boosted antioxidant enzyme activities and reduced MDA and H_(2)O_(2) contents in chilling-stressed rice plants,indicating less oxidative stress and improved tolerance.Principal component analysis showed that among these priming agents,H_(2)O_(2),SA,and CA are most effective in chilling stress mitigation.Therefore,using seed-treating chemicals to combat the effect of chilling stress can help rice seedlings grow better in the winter season.展开更多
基金funded by the Natural Science Foundation of Shandong Province(ZR2020MC144)the Scientific Research Training Program for Undergraduates of Qufu Normal University(XJ2024016).
文摘Strontium has similar chemical properties to calcium and has recently been recognized as a non-essential beneficial element for plants.In order to compare the effects of strontium and calcium on improving salt tolerance of Chinese cabbage during the germination stage,2,4,and 8 mmol/L of SrCl_(2),CaCl_(2) or an equimolar mixture of both were added separately to a 150 mmol/L NaCl solution.The results showed that Ca-Sr addition significantly increased seed viability,seed vigor,seed germination rate and seed germination uniformity of Chinese cabbage compared with the salt-control group.The differences in germination percentage(GP)and germination energy(GE)among the Ca-addition and Sr-addition groups were not significant,and the differences in coefficient of rate of germination(CRG),index of rate of germination(IRG)and coefficient of variation of the germination time(CVT)were relatively small,but clear differences were observed in germination index(GI),vigor index(VI)and coefficient of uniformity of germination(CUG).The results of GI and VI indicated that the higher the concentration of Ca-addition or Sr-addition,the more significant the enhancement of seed vigor.Under saline stress(150 mmol/L NaCl),the Ca-Sr co-addition outperformed Sr-treatment alone,and Ca-addition achieved the highest seed vigor at equivalent concentrations.Furthermore,all Ca-Sr treatments significantly enhanced the uniformity of Chinese cabbage sprouts exposed to 150 mM NaCl,with the best uniformity improved by the addition of 2 and 4 mmol/L SrCl_(2).Ca-Sr treatments increased the salt tolerance of Chinese cabbage sprouts during the germination stage mainly because the Ca2+and Sr2+significantly enhanced plasma membrane stability and reduced oxidative stress(as indicated by decreased contents of malondialdehyde and O2⋅-contents)in sprouts.The decrease of soluble sugar and proline content caused by Ca-Sr addition implies that elevated levels of these osmolytes were not the primary contributors to improved seed germinability in Chinese cabbage.These findings demonstrate that Sr is a beneficial element for enhancing salt tolerance in plants,laying a theoretical foundation for the development and application of strontium in agriculture.
文摘[Objective] This study aimed to investigate the effects of salt stress on seed germination and seedling growth in radish（Raphanus sativus L.）.[Method] First,effects of soaking seeds with clear water and different concentrations of saline solution were compared; then,different concentrations of salt solution （0.2％-1.0％） was used to treat radish during the whole germination period.[Result] soaking seeds with clear water can improve the germination indicators of radish seeds exposed to high salt stress; low salt concentration（0.2％,0.4％） had little effect on the growth indicators of radish seedlings,and 0.2％ salt concentration can even promote the growth of radish seedlings,however,the high salinity had a bad inhibition on seedling growth,the higher the salt concentration,the stronger the inhibition.[Conclusion] According to the comprehensive comparison,the order of the six varieties in salt-tolerance was as follows：Xinxiayuanbai＞W1029,Xiayuanbai ＞ Nanyangbailizhi,Chunyidianhong＞ Sijihong 3.
文摘Drought is considered one of the leading abiotic constraints to agricultural crop production globally.Present study was conducted to assess the effects of different drought treatments(viz.Control,10%PEG,and 20%PEG)on seed germination,germination indices,seedling traits,and drought tolerance indices of sesame.Our results showed that maximum reduction in the studied parameters was observed at higher PEG concentration(i.e.,20%PEG).As compared to control,the drought treatments viz.10%and 20%PEG decreased the values for germination indices,such as germination percentage,coefficient of variation of germination time,germination index,and seedling vigor index.Similarly,for seedling traits,the values were decreased for root length,shoot length,root shoot ratio,root fresh weight,shoot fresh weight,root dry weight and shoot dry weight under 10%and 20%PEG treatments significantly in comparison with control.Furthermore,relative to control,the values for drought tolerance indices,such as germination drought tolerance index,root length drought tolerance index,shoot length drought tolerance index,total seedling length drought tolerance index,root fresh weight drought tolerance index,shoot fresh weight drought tolerance index,total fresh weight drought tolerance index,root dry weight drought tolerance index,shoot dry weight drought tolerance index and total dry weight drought tolerance index were also reduced under 10%and 20%PEG treatments,respectively.Our results confirms that drought impact on seed germination and seedling traits could be quantified by using different indices which can further help to design drought adaptation and mitigation strategies.Based on these results it can be concluded that germination indices,seedling traits,and drought tolerance indices have great potential to simulate drought stress impacts on different crop traits thus they should be used in all kinds of stress related studies.
文摘Chilling is one of the major abiotic stresses for plants,especially for rice cultivation.Many essential metabolic processes for growth and development are temperature-dependent.In that case,reducing the negative effects of cold stress using exogenous chemicals is a possible option.Therefore,the current study examined the effects of pre-sowing seed treatment with different chemicals,viz.hydrogen peroxide(H_(2)O_(2)),salicylic acid(SA),calcium chloride(CaCl_(2)),thiourea(TU),and citric acid(CA)on the germination of rice seeds(cv.BRRI dhan28)under chilling environments.Rice seeds were soaked in distilled water(control),10 mM CA,2 mM SA,10 mM CaCl_(2),10 mM H_(2)O_(2),and 10 mM TU solutions for 24 h.After that,seeds were exposed to chilling stress by incubating at 4±1℃ for 8 h,followed by at 25±2℃ for 16 h for 7 days.Exposure to chilling stress significantly reduced thefinal germination percent(13.6%),germination rate index(36.0%),coefficient of the velocity(25.0%),shoot fresh weight(44.4%),and root fresh weight(60.5%).Moreover,chilling induced oxidative damage and reduced the activity of antioxidant enzymes(catalase and ascorbate peroxidase).In contrast,treatments with H_(2)O_(2),SA,CaCl_(2),TU,and CA considerably enhanced germination indices and seedling growth compared to chilling stress condi-tions.The study showed that priming with H_(2)O_(2),SA,CaCl_(2),TU,and CA significantly boosted antioxidant enzyme activities and reduced MDA and H_(2)O_(2) contents in chilling-stressed rice plants,indicating less oxidative stress and improved tolerance.Principal component analysis showed that among these priming agents,H_(2)O_(2),SA,and CA are most effective in chilling stress mitigation.Therefore,using seed-treating chemicals to combat the effect of chilling stress can help rice seedlings grow better in the winter season.
