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On-orbit refueling robust mission scheduling with uncertain duration for geosynchronous orbit spacecraft
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作者 Shuai YIN Chuanjiang LI +3 位作者 Edoardo FADDA Yanning GUO Guangtao RAN Paolo BRANDIMARTE 《Chinese Journal of Aeronautics》 2026年第1期410-424,共15页
With the increasing number of geosynchronous orbit satellites with expiring lifetime,spacecraft refueling is crucial in enhancing the economic benefits of on-orbit services.The existing studies tend to be based on pre... With the increasing number of geosynchronous orbit satellites with expiring lifetime,spacecraft refueling is crucial in enhancing the economic benefits of on-orbit services.The existing studies tend to be based on predetermined refueling duration;however,the precise mission scheduling solution will be difficult to apply due to uncertain refueling duration caused by orbital transfer deviations and stochastic actuator faults during actual on-orbit service.Therefore,this paper proposes a robust mission scheduling strategy for geosynchronous orbit spacecraft on-orbit refueling missions with uncertain refueling duration.Firstly,a robust mission scheduling model is constructed by introducing the budget uncertainty set to describe the uncertain refueling duration.Secondly,a hybrid harris hawks optimization algorithm is designed to explore the optimal mission allocation and refueling sequences,which combines cubic chaotic mapping to initialize the population,and the crossover in the genetic algorithm is introduced to enhance global convergence.Finally,the typical simulation examples are constructed with real-mission scenarios in three aspects to analyze:performance comparisons with various algorithms;robustness analyses via comparisons of different on-orbit refueling durations;investigations into the impacts of different initial population strategies on algorithm performance,demonstrating the proposed mission scheduling framework's robustness and effectiveness by comparing it with the exact mission scheduling. 展开更多
关键词 geosynchronous orbit(GEO) Hybrid Harris Hawks Optimization algorithm(HHHO) Mission scheduling On-orbit refueling Robust optimization
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Multi-objective evolutionary optimization for geostationary orbit satellite mission planning 被引量:4
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作者 Jiting Li Sheng Zhang +1 位作者 Xiaolu Liu Renjie He 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2017年第5期934-945,共12页
In the past few decades, applications of geostationary orbit (GEO) satellites have attracted increasing attention, and with the development of optical technologies, GEO optical satellites have become popular worldwide... In the past few decades, applications of geostationary orbit (GEO) satellites have attracted increasing attention, and with the development of optical technologies, GEO optical satellites have become popular worldwide. This paper proposes a general working pattern for a GEO optical satellite, as well as a target observation mission planning model. After analyzing the requirements of users and satellite control agencies, two objectives are simultaneously considered: maximization of total profit and minimization of satellite attitude maneuver angle. An NSGA-II based multi-objective optimization algorithm is proposed, which contains some heuristic principles in the initialization phase and mutation operator, and is embedded with a traveling salesman problem (TSP) optimization. The validity and performance of the proposed method are verified by extensive numerical simulations that include several types of point target distributions. 展开更多
关键词 geostationary orbit (GEO) satellitemission planning multi-objective optimization evolutionary genetic
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Model calculation of air-sea heat fluxes over ocean area near China with GEOS DAS system
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作者 周明煜 钱粉兰 《Acta Oceanologica Sinica》 SCIE CAS CSCD 1999年第3期401-411,共11页
The sensible and latent heat fluxes over the ocean area near China were calculated and analyzed by usingthe Goddard Earth Observing System (GEOS) - four-dimensional Data Assimilation System (DAS). The calculated resul... The sensible and latent heat fluxes over the ocean area near China were calculated and analyzed by usingthe Goddard Earth Observing System (GEOS) - four-dimensional Data Assimilation System (DAS). The calculated results showed that the sensible heat flux had its large value in winter and autumn , small value in spring and summer overthe ocean area near China. In winter, the sensible heat flux increased distinctly with latitude, and its isolines were verycrowded. Over the ocean area east of Taiwan Province and south of Japan, the direction of isoline was from southwestto northeast. In the South China Sea the sensible heat flux was lower than that of surrounding ocean areas,and its isoline was distributed into a type of an inverted trough. In autumn and winter, the maximum center of latent heat flux appeared over the ocean area northeast of Taiwan Province and south and southeast of Japan, meanwhile, the isoline wasin the direction of southwest to northeast. In spring and summer, the latent heat flux had minimum value in the Huanghai Sea. At the same time, the maximum value center of latent heat flux appeared over the ocean area south of Japan inspring. 展开更多
关键词 geos DAS system the ocean area near China sensible heat flux latent heat flux
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Re-examining Geos Neighbourhood for Its Energy Self-sustained Ability Through Ecological Footprints, Net Energy, and Life Cycle Assessment Tools
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作者 Mengxiang HU 《Agricultural Science & Technology》 CAS 2014年第8期1384-1389,共6页
There are mounting evidences that human consume significant quantities of resources and have a major impact on the environment. Some research indicates that since the 1980s human demands on the biosphere have exceeded... There are mounting evidences that human consume significant quantities of resources and have a major impact on the environment. Some research indicates that since the 1980s human demands on the biosphere have exceeded its regen-errative capacity. One way to solve this problem is to minimize the inappropriate ex-ploitation from environment. This essay wil focus on energy self-sustained project, which is a specific way to reduce energy requirements. Energy self-sustained project refers to that energy production is equal to the amount consumed. Three concepts are quoted in the fol-lowing to assess whether the project achieve zero-energy or not. First, ecological footprint, which provides an indication of the human load on the biosphere, is uti-lized to measure the inputs and outputs of the bioregion, which is also beneficial for defining the potential energy. Another one is life cycle assessment, which evaluates environmental load that relates to the entire life periodic system of a product, is helpful to measure the products used in the energy self sustained project. In addi-tion, net energy, and gross resource abundance, definitions for selection, or hierar-chy of different energy resources, can evaluate the new energy resources in project. Geos Neighbourhood, located in colorado, was planned as the largest net-zero energy neighbourhood in the United States. To meet the energy self sustainability, earth and sun power completely sustain the community's energy needs, and re-place al fossil fuels. Compared with the traditional communities, Geos Neighbour-hood minimize the adverse impact on the environment. As tools for assessment, ecological footprint, life circle assessment, and net energy, are al used to analyse the planning and design principles in the neighbourhood. By the research, the de-sign principles and energy use in Geos Neighbourhood wil be re-examined that whether the zero energy project achieves the reduction of ecological footprint, and energy self sustainability. In addition, life circle assessment wil re-examine the ma-terials used in the community also. Final y, the concept of 'net energy' wil test solar energy and earth power which is the major energy used in Geos Neighbour-hood. 展开更多
关键词 Energy self sustained Ecological footprints Net energy Life cycle assessment geos Neiqhbourhood
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Simulation and Analysis of the Impacts of Anisotropic Ionospheric Scintallition on Geosynchronous Synthetic Aperture Radar
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作者 Yinhui Xu Xichao Dong +1 位作者 Cheng Hu Yuanhao Li 《Journal of Beijing Institute of Technology》 EI CAS 2017年第1期115-120,共6页
The impacts of ionospheric scintillation on geosynchronous synthetic aperture radar(GEO SAR)focusing is studied based on the multiple phase screen(MPS)theory.The power spectrum density of electron irregularities i... The impacts of ionospheric scintillation on geosynchronous synthetic aperture radar(GEO SAR)focusing is studied based on the multiple phase screen(MPS)theory.The power spectrum density of electron irregularities is first modified according to the ionospheric anisotropy.Then propagation wave equations in random medium are deduced in the case of oblique incidence in GEO SAR.The amplitude and phase errors induced by the random electron fluctuations are generated by the iterated MPS simulations and are superimposed into the GEO SAR signals.Through the following imaging and evaluation,the effects of the anisotropic ionospheric scintallition on GEO SAR are assessed.At last,the optimized integration time under different ionospheric scintillation conditions are recommended through Monte Carlo experiments.It is concluded that,greater ionospheric fluctuations and longer integration time will result in more severe deterioration,even no focus at all in the worst case. 展开更多
关键词 ANISOTROPY FOCUSING geosynchronous synthetic aperture radar (GEO SAR) ionospher-ic scintillation multiple phase screen
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基于GeoServer和OpenLayers技术的地理信息系统在市容环卫行业中的应用 被引量:5
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作者 申晓晓 孙倩 《环境卫生工程》 2016年第3期75-77,共3页
运用开源软件Geo Server和Open Layers技术,建立与市容环卫行业相关的地理信息系统,实现直观可视化的地理信息展示和管理。介绍了Geo Server和Open Layers技术以及开发方案,搭建出环卫网络管理系统的整体架构,并论述系统在环卫行业中的... 运用开源软件Geo Server和Open Layers技术,建立与市容环卫行业相关的地理信息系统,实现直观可视化的地理信息展示和管理。介绍了Geo Server和Open Layers技术以及开发方案,搭建出环卫网络管理系统的整体架构,并论述系统在环卫行业中的具体应用。 展开更多
关键词 地理信息系统 市容环卫行业 GEO SERVER Open LAYERS
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基于GeoServer的地学数据共享系统设计与实现 被引量:5
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作者 郭鹏程 李广宇 《工程建设与设计》 2017年第15期142-145,共4页
地学数据是国民经济建设和社会发展的重要支撑,数据资料共享是推进地学研究的重要手段。论文针对当前地学数据安全共享面临的问题,综合运用计算机网络、GIS和数据库技术,提出了一个基于Open GIS规范的共享解决方案,设计了共享系统的整... 地学数据是国民经济建设和社会发展的重要支撑,数据资料共享是推进地学研究的重要手段。论文针对当前地学数据安全共享面临的问题,综合运用计算机网络、GIS和数据库技术,提出了一个基于Open GIS规范的共享解决方案,设计了共享系统的整体框架、数据库结构及系统的详细功能模块,并以海南省1:250万基础地质空间数据共享为例,基于开源软件Geo Server进行了系统实现,使多源、异构的空间数据能够有机地整合并实现信息的共享和交互。可为解决地学数据共享中的问题提供参考和借鉴。 展开更多
关键词 GEO SERVER 地学数据 OPENGIS 数据共享
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基于GeoStudio的山东某水库防渗问题研究 被引量:2
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作者 梁洪 王凌书 《甘肃水利水电技术》 2015年第4期19-24,共6页
对Geo Studio软件的功能和地下水渗流分析SEEP/W模块做了介绍。根据工程地质勘察资料和水文地质资料,对水库大坝模型运用SEEP/W模块,模拟水库蓄水后坝基的渗流情况。从计算结果可以看出,采用防渗墙处理后的渗流量得到了有效控制,满足大... 对Geo Studio软件的功能和地下水渗流分析SEEP/W模块做了介绍。根据工程地质勘察资料和水文地质资料,对水库大坝模型运用SEEP/W模块,模拟水库蓄水后坝基的渗流情况。从计算结果可以看出,采用防渗墙处理后的渗流量得到了有效控制,满足大坝的防渗设计要求,验证了防渗墙的防渗效果还与防渗墙的深度有很大关系。 展开更多
关键词 GEO STUDIO 土石坝 防渗墙 坝基渗流
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Dynamic Connectivity in Cislunar Communication Networking Based on Geosynchronous Orbit Relay Satellites 被引量:4
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作者 Wu Xiaobo Zhou Xianwei Lin Fuhong 《China Communications》 SCIE CSCD 2012年第11期41-53,共13页
The architecture of cislunar multi-hop communication networks, which focuses on the requirements of lunar full-coverage and continuous cislunar communications, is presented on the basis of Geosynchronous Orbit (GEO) s... The architecture of cislunar multi-hop communication networks, which focuses on the requirements of lunar full-coverage and continuous cislunar communications, is presented on the basis of Geosynchronous Orbit (GEO) satellite network relays. According to the geographical distribution of the forthcoming Chinese Deep Space Measuring and Controlling Network (DSMCN), two networking schemes are proposed and two elevation angle optimization models are established for locating GEO relay satellites. To analyze the dynamic connectivity, a dynamic network model is constructed with respect to the time-varying characteristics of cislunar trunk links. The advantages of the two proposed schemes, in terms of the Connectivity Rate (CR), Interruption Frequency (IF), and Average Length of Connecting Duration (ALCD), are corroborated by several simulations. In the case of the lunar polar orbit constellation case, the gains in the performance of scheme I are observed to be 134.55%, 117.03%, and 217.47% compared with DSMCN for three evaluation indicators, and the gains in the performance of scheme II are observed to be 238. 22%, 240.40%, and 572.71%. The results validate that the connectivity of GEO satellites outperforms that of earth facilities significantly and schemes based on GEO satellite relays are promising options for cislunar multi-hop communication networking. 展开更多
关键词 cislunar communication networking dynamic connectivity dynamic network model GEO relay satellites lunar full coverage
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Leica Geosystems重组
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作者 林之(摘) 《遥感信息》 CSCD 2006年第2期4-4,共1页
由于转归Hexagen公司所属,所以瑞士Leica Geosystems公司在组织上进行了调整。在不影响总的战略方向的同时,公司改组为二个分部,其负责人分别向Hexagen AB公司董事长和CEO汇报工作。原用户产品部重新命名为量测工具部。重点放在新的... 由于转归Hexagen公司所属,所以瑞士Leica Geosystems公司在组织上进行了调整。在不影响总的战略方向的同时,公司改组为二个分部,其负责人分别向Hexagen AB公司董事长和CEO汇报工作。原用户产品部重新命名为量测工具部。重点放在新的第六代激光测距产品DISTRO上。总部设在美国亚特兰大的地理空间成像分部重点将放在软件上并准备进入测高市场。原测量和工程部,高精度测量部。 展开更多
关键词 LEICA systems公司 高精度测量 重组 AB公司 激光测距 空间成像 亚特兰大 GEO 负责人
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基于GEO数据库与机器学习回归分析的p53和pRb表达水平与宫颈癌相关性分析
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作者 陈群 苏峻瑶 从丽 《机器人外科学杂志(中英文)》 2026年第1期167-173,共7页
目的:通过GEO数据库与机器学习回归分析探究肿瘤抑制蛋白p53(p53)和视网膜母细胞瘤蛋白(pRb)表达水平与宫颈癌严重程度的相关性。方法:根据基因表达综合数据库(GEO)的宫颈癌全球流行病学数据,分析TP53和RB1基因与宫颈癌的相关性。同时,... 目的:通过GEO数据库与机器学习回归分析探究肿瘤抑制蛋白p53(p53)和视网膜母细胞瘤蛋白(pRb)表达水平与宫颈癌严重程度的相关性。方法:根据基因表达综合数据库(GEO)的宫颈癌全球流行病学数据,分析TP53和RB1基因与宫颈癌的相关性。同时,选取2022年1月—2024年12月于江苏省人民医院宿迁医院治疗的100例宫颈癌患者作为研究对象,收集癌细胞分化程度、癌症分期、患者年龄等临床资料,并从生化检验科室获取p53和pRb表达水平。结果:通过GEO数据库分析,宫颈癌发病年龄集中在35~62岁,前5年生存率呈显著下降趋势,5年生存率约为40%。TP53和RB1基因与宫颈癌相关,SCC-Ag、p53、pRb的表达水平在不同癌细胞分化程度中存在区别,差异有统计学意义(P<0.05),且癌细胞分化程度与SCC-Ag、p53、pRb表达水平分别呈正相关、负相关、负相关。p53和pRb均与SCC-Ag呈负相关。结论:p53和pRb表达水平与宫颈癌严重程度密切相关,对临床治疗预后具有参考价值。 展开更多
关键词 GEO数据库 机器学习回归 P53 PRB 宫颈癌 严重程度
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Mathematical prototypes for collocating geostationary satellites 被引量:6
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作者 LI HengNian GAO ZhaoZhao +3 位作者 LI JiSheng LI QuanJun XUE Dan LI DongLin 《Science China(Technological Sciences)》 SCIE EI CAS 2013年第5期1086-1092,共7页
Collocating geostationary satellites sharing the same position is much demanded for satellite operation recently,the separation strategies are adopted to safeguard the satellites collocated of leaving the relative dis... Collocating geostationary satellites sharing the same position is much demanded for satellite operation recently,the separation strategies are adopted to safeguard the satellites collocated of leaving the relative distance beyond collision with different sets of orbit parameters.This paper presents the mathematical prototypes which establish the allowable relative distance with uncertainty of orbital determination(OD),as well as the orbital element offset for each pair of collocated satellites,and puts forward algorithms to build such relationship to face the challenge of putting three satellites sharing the same position,the algorithms to allocate the longitude,eccentricity and inclination for each satellite are also given to ascertain that the mathematical prototypes are the guide specification to design collocation strategy for geostationary satellites. 展开更多
关键词 geostationary satellite (GEO) satellite operation satellite collocation separation strategies
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肉豆蔻醚摄入对帕金森病及抑郁症的毒性机制分析数据集
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作者 王昕 张瑜 +1 位作者 袁博 邸多隆 《天然产物研究与开发》 北大核心 2026年第2期450-453,共4页
本数据集分析了食品香料肉豆蔻中的肉豆蔻醚的潜在神经毒性,整合PubChem、TCMSP、GeneCards、HERB、OMIM、STRING、DAVID、NCBI GEO与PDB等公共资源,形成“化合物—靶点—网络/富集—表达验证—分子对接”的多层数据链。数据集包含8个表... 本数据集分析了食品香料肉豆蔻中的肉豆蔻醚的潜在神经毒性,整合PubChem、TCMSP、GeneCards、HERB、OMIM、STRING、DAVID、NCBI GEO与PDB等公共资源,形成“化合物—靶点—网络/富集—表达验证—分子对接”的多层数据链。数据集包含8个表格(肉豆蔻醚ADMET信息、肉豆蔻醚靶点、帕金森病与抑郁症相靶点及交集靶点、GEO数据库验证肉豆蔻醚诱导的帕金森病和抑郁症的差异表达基因)和5张图(肉豆蔻醚摄入对帕金森病及抑郁症的毒性机制分析的PPI网络图、GO和KEGG分析结果、GEO数据库验证的肉豆蔻醚诱导帕金森病和抑郁症中相关靶点表达差异统计分析图,以及核心靶点的分子对接结果图)。该数据集可为帕金森病和抑郁症的共病风险信号筛查以及肉豆蔻醚相关的食品质量控制策略提供参考依据。 展开更多
关键词 肉豆蔻醚 帕金森病 抑郁症 网络药理学 GEO数据库 数据集
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Research progress on geosynchronous synthetic aperture radar 被引量:7
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作者 Cheng Hu Zhiyang Chen +2 位作者 Yuanhao Li Xichao Dong Stephen Hobbs 《Fundamental Research》 CAS 2021年第3期346-363,共18页
Based on its ability to obtain two-dimensional(2D)high-resolution images in all-time and all-weather conditions,spaceborne synthetic aperture radar(SAR)has become an important remote sensing technique and the study of... Based on its ability to obtain two-dimensional(2D)high-resolution images in all-time and all-weather conditions,spaceborne synthetic aperture radar(SAR)has become an important remote sensing technique and the study of such systems has entered a period of vigorous development.Advanced imaging modes such as radar interferometry,tomography,and multi-static imaging,have been demonstrated.However,current in-orbit spaceborne SARs,which all operate in low Earth orbits,have relatively long revisit times ranging from several days to dozens of days,restricting their temporal sampling rate.Geosynchronous SAR(GEO SAR)is an active research area because it provides significant new capability,especially its much-improved temporal sampling.This paper reviews the research progress of GEO SAR technologies in detail.Two typical orbit schemes are presented,followed by the corresponding key issues,including system design,echo focusing,main disturbance factors,repeat-track interferometry,etc,inherent to these schemes.Both analysis and solution research of the above key issues are described.GEO SAR concepts involving multiple platforms are described,including the GEO SAR constellation,GEO-LEO/airborne/unmanned aerial vehicle bistatic SAR,and formation flying GEO SAR(FF-GEO SAR).Due to the high potential of FF-GEO SAR for three-dimensional(3D)deformation retrieval and coherence-based SAR tomography(TomoSAR),we have recently carried out some research related to FF-GEO SAR.This research,which is also discussed in this paper,includes developing a formation design method and an improved TomoSAR processing algorithm.It is found that GEO SAR will continue to be an active topic in the aspect of data processing and multi-platform concept in the near future. 展开更多
关键词 geosynchronous synthetic aperture radar(GEO SAR) Orbit scheme Disturbance factors Echo focusing Formation flying
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Safe rendezvous scenario design for geostationary satellites with collocation constraints 被引量:5
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作者 Ya-Zhong Luo Zhen-Jiang Sun 《Astrodynamics》 2017年第1期71-83,共13页
Rendezvous on the geostationary orbit(GEO)is much more complex than that on the low earth orbit and has a higher critical requirement for safety performance.This paper presents a safe scenario design method for GEO re... Rendezvous on the geostationary orbit(GEO)is much more complex than that on the low earth orbit and has a higher critical requirement for safety performance.This paper presents a safe scenario design method for GEO rendezvous proximity missions where the safety constraint of a collocated satellite is considered.A recently proposed quantitative index considering trajectory uncertainty is introduced to analyze the safety performance of the scenario parameters including the V-bar keeping positions and the fly-by trajectory radius.Furthermore,an exhaustive analysis is performed to find the dangerous regions of the V-bar keeping positions and the appropriate semi-major axis of the fly-by ellipse,considering the safety requirements of both the target and the collocated satellite.A geometry method is then developed for designing a feasible and suboptimal safe rendezvous scenario.The method is tested by designing four rendezvous scenarios with±V-bar approach directions respectively in the situations with and without one collocated satellite.Safety performance and velocity increments of the scenarios are compared and a conclusion is reached that the collocated satellite has a significant influence on the scenario design. 展开更多
关键词 geostationary orbit(GEO) rendezvous and docking(RVD) safety performance satellite collocation
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肝内胆管细胞癌自噬相关基因的筛选和预后模型的构建
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作者 张映林 张文锋 +1 位作者 苏代文 黎锋 《现代肿瘤医学》 2026年第3期329-337,共9页
目的:基于自噬相关基因(autophagy-related genes,ARGs),利用生物信息学的方法筛选出与肝内胆管细胞癌(intrahepatic cholangiocarcinoma,ICC)预后相关的差异基因并由此建立预后模型。方法:从癌症基因组图谱(The Cancer Genome Atlas,TC... 目的:基于自噬相关基因(autophagy-related genes,ARGs),利用生物信息学的方法筛选出与肝内胆管细胞癌(intrahepatic cholangiocarcinoma,ICC)预后相关的差异基因并由此建立预后模型。方法:从癌症基因组图谱(The Cancer Genome Atlas,TCGA)和基因表达综合数据库(Gene Expression Omnibus,GEO)数据库中下载ICC组织的RNA转录组测序数据和相应的临床资料。筛选出ICC患者癌组织与正常肝组织中差异表达的ARGs后,再利用单因素和LASSO Cox回归分析建立与ICC患者预后相关的预测模型。接着,利用Kaplan-Meier分析、受试者工作特征曲线、单变量和多变量Cox回归分析验证该预测模型与临床特征的相关性。之后,再利用独立的GEO数据库队列进一步验证了该预测模型的有效性。最后,利用实时荧光定量聚合酶链式反应法检测体外ICC细胞中预测模型中ARGs的mRNA表达。结果:从ICC组织中共筛选出141个差异表达的ARGs。在这些差异表达基因中,鉴定出4个与生存相关的基因(SPHK1,BNIP3,BAX和BNIP3L)。然后,利用这4个基因组成的预测模型将ICC患者分为低风险组和高风险组。我们发现,低风险组的ICC患者的总生存时间明显高于高风险组,且该预后模型组成的风险评分被证实是ICC患者独立的预测因子。此外,该模型的有效性在GEO独立队列中得到了进一步验证。最后,我们发现在人肝内胆管上皮细胞系(HIBEC)中,SPHK1、BNIP3和BNIP3L的mRNA表达显著低于ICC细胞系(CCLP1和HuCCT-1),BAX的mRNA表达显著高于ICC细胞系(CCLP1和HuCCT-1)。结论:基于自噬相关基因建立的预后模型可有效预测ICC患者的生存,这将为ICC患者提供潜在的预后相关的生物标志物和治疗靶点。 展开更多
关键词 肝内胆管细胞癌 自噬基因 预后模型 TCGA GEO
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系统性红斑狼疮相关分子标志物的探索与验证
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作者 谢雅洁 王玉洁 +3 位作者 杨翼佳 胡皓月 张小红 王可 《医学理论与实践》 2026年第2期191-195,224,共6页
目的:通过芯片表达数据和临床样本鉴定并验证系统性红斑狼疮(SLE)相关分子标志物,并初步探索其分子机制。方法:采用GEO数据库GSE61635和GSE72754进行生物信息学分析,鉴定SLE相关枢纽基因并进行基因功能富集性分析。同时收集SLE患者和健... 目的:通过芯片表达数据和临床样本鉴定并验证系统性红斑狼疮(SLE)相关分子标志物,并初步探索其分子机制。方法:采用GEO数据库GSE61635和GSE72754进行生物信息学分析,鉴定SLE相关枢纽基因并进行基因功能富集性分析。同时收集SLE患者和健康对照组临床血液样本(各50例),采用RT-qPCR实验进行枢纽基因mRNA表达水平差异性验证。结果:共鉴定10个SLE相关枢纽基因SLC4A1、EPB42、AHSP、GYPB、ALAS2、HEMGN、HBD、CA1、FECH和KLF1。主要参与细胞内稳态、血红蛋白代谢、造血功能调节、跨膜转运蛋白活性调节等生物功能及线粒体、氨基酸等代谢相关通路调节。临床样本验证结果表明,枢纽基因在SLE组的mRNA表达水平均低于健康对照组,与GEO数据库分析结果一致。结论:枢纽基因可能通过血液病理学和代谢异常途径导致免疫功能失调,进而对SLE的发生发展产生关键作用,是SLE早期临床诊断及预后预测评估的潜在个体化生物标志物。 展开更多
关键词 系统性红斑狼疮 GEO数据库 枢纽基因 生物标志物
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基于生物信息学和动物实验探讨苓桂术甘汤改善心肌梗死后心肌纤维化的潜在机制
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作者 赵婉竹 任涵 +4 位作者 王舒舒 蔡瑞 黄圣意 张远红 黄金玲 《天然产物研究与开发》 北大核心 2026年第2期412-424,305,共14页
利用生物信息学和体内实验研究苓桂术甘汤(Linggui Zhugan Decoction,LZD)对急性心肌梗死(acute myocardial infarction,AMI)后心肌纤维化(myocardial fibrosis,MF)的潜在作用机制。在TCMSP数据库获取LZD活性成分及对应靶点,在GeneCards... 利用生物信息学和体内实验研究苓桂术甘汤(Linggui Zhugan Decoction,LZD)对急性心肌梗死(acute myocardial infarction,AMI)后心肌纤维化(myocardial fibrosis,MF)的潜在作用机制。在TCMSP数据库获取LZD活性成分及对应靶点,在GeneCards、OMIM、TTD和GEO数据库收集疾病靶点;运用Cytoscape软件绘制“药物-成分-靶点”网络;STRING网站构建蛋白互作网络;R语言进行富集分析。结扎小鼠左前降支冠状动脉构建AMI后MF模型进行实验验证,4周后使用多普勒超声成像仪检测小鼠心功能;HE、Masson染色观察心肌组织形态学和胶原纤维沉积变化情况;Western blot法检测心肌纤维化和核心靶点蛋白表达水平;免疫组化法检测心肌组织中α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)水平。生物信息学结果显示,LZD有效成分对应靶点、疾病靶点和GSE97358差异基因三者的交集靶点共33个;核心活性成分为槲皮素、山柰酚、β-谷甾醇等。实验结果表明,LZD可以显著改善AMI后MF小鼠心功能和病理形态学变化,且间质胶原纤维沉积显著减少;小鼠心肌组织中纤维化相关蛋白α-SMA、胶原蛋白Ⅰ(collagenⅠ,ColⅠ)、ColⅢ、基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)以及核心靶点白细胞介素-1β(interleukin-1β,IL-1β)、前列腺素内过氧化物合酶2(prostaglandin-endoperoxide synthase 2,PTGS2)和CXC趋化因子配体8(CXC chemokine ligand 8,CXCL8)的蛋白表达水平显著下降,过氧化物酶体增殖物激活受体γ(peroxisome proliferators activated receptor gamma,PPARγ)和B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)蛋白表达水平显著上升。综上所述,LZD可以调节多个信号通路及靶点,且显著改善小鼠AMI后MF,延缓AMI转化为慢性心衰的病理进程,其作用机制可能与生物信息学筛选得到的靶点密切相关。 展开更多
关键词 急性心肌梗死 心肌纤维化 苓桂术甘汤 网络药理学 GEO数据库
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2025年大模型在全球信息传播中的应用图景
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作者 林润 张洪忠 +1 位作者 王诗然 张思滢 《传媒》 2026年第3期8-14,共7页
大模型技术的广泛应用,正在信息传播领域引发内容生成、传播效率与用户交互的深刻变革。本文系统梳理了2025年大模型在全球信息传播领域的应用图景。大模型已超越工具属性,演进为支撑信息生产、分发与交互全链条的“新型基础设施”,驱... 大模型技术的广泛应用,正在信息传播领域引发内容生成、传播效率与用户交互的深刻变革。本文系统梳理了2025年大模型在全球信息传播领域的应用图景。大模型已超越工具属性,演进为支撑信息生产、分发与交互全链条的“新型基础设施”,驱动传播模式向“人机协同”深刻转型。其在全球呈现基础设施化与平台化趋势,并通过GEO机制重塑分发逻辑;用户市场向非主流语种拓展并深入上游商用环节;在内容生产端实现了从文本、图像、视频到代码与应用的全方位赋能;主流媒体则普遍采用“机器辅助、人工把关”的人机协同模式。展望未来,大模型作为智能体将进一步赋能自动化深度处理,但同时也对信息伦理、算法透明度与文化主权提出了新的挑战。 展开更多
关键词 大模型 信息传播 智能传播 智能体 GEO
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基于GEO数据库探讨翼状胬肉线粒体代谢基因AKT1与ATP5F1及BAG3表达与作用
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作者 王诗逸 王婧 +1 位作者 王华 陈玉萍 《国际眼科杂志》 2026年第3期398-404,共7页
目的:探讨丝氨酸/苏氨酸激酶1(AKT1)、ATP合酶F1亚基(ATP5F1)及Bcl-2相关抗凋亡基因3(BAG3)三者在翼状胬肉发生发展中的表达差异及作用机制。方法:通过GEO数据库获取翼状胬肉相关基因表达数据,筛选差异表达基因;利用String数据库构建蛋... 目的:探讨丝氨酸/苏氨酸激酶1(AKT1)、ATP合酶F1亚基(ATP5F1)及Bcl-2相关抗凋亡基因3(BAG3)三者在翼状胬肉发生发展中的表达差异及作用机制。方法:通过GEO数据库获取翼状胬肉相关基因表达数据,筛选差异表达基因;利用String数据库构建蛋白相互作用(PPI)网络,结合Cytoscape软件筛选核心靶点;采用GO和KEGG通路富集分析线粒体代谢相关通路特征。收集翼状胬肉手术患者的胬肉头部、体部组织标本,HE染色观察两组组织病理形态差异,免疫组化法检测AKT1、ATP5F1、BAG3的蛋白表达水平。结果:GEO数据库分析共筛选出差异表达基因1264个,其中上调585个、下调679个。GO富集分析显示,生物学过程、细胞组分及分子功能层面均存在线粒体相关通路的显著富集;KEGG富集分析以氧化磷酸化、化学致癌-活性氧簇通路差异最为显著。收集翼状胬肉手术患者28例28眼的胬肉头部、体部组织标本,其中男7例7眼,女21例21眼,平均年龄69.32±8.98岁。HE染色结果显示,胬肉头部较体部上皮细胞异型增生更明显、基质纤维排列更紊乱,且炎症细胞浸润更显著。免疫组化结果证实,AKT1、ATP5F1、BAG3在胬肉头部的蛋白表达水平显著低于体部(均P<0.05)。结论:线粒体代谢相关基因AKT1、ATP5F1、BAG3在翼状胬肉中存在明显表达差异,且与病变组织的病理进展密切相关,其可能通过调控线粒体能量代谢参与翼状胬肉的发生发展,为该病的机制研究及靶向治疗提供新的实验依据与思路。 展开更多
关键词 翼状胬肉 GEO数据库 线粒体代谢 丝氨酸/苏氨酸激酶1(AKT1) ATP合酶F1亚基(ATP5F1) Bcl-2相关抗凋亡基因3(BAG3)
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