BACKGROUND Gallstone(GS),a prevalent biliary disorder,is associated with bile stasis,infection,and cholesterol metabolism.Recent research highlights the potential role of bile microbiota in GS pathogenesis.This is a c...BACKGROUND Gallstone(GS),a prevalent biliary disorder,is associated with bile stasis,infection,and cholesterol metabolism.Recent research highlights the potential role of bile microbiota in GS pathogenesis.This is a case control study conducted at Jinshan Hospital between 2022 and 2023.AIM To investigate the relationship between bile microbiota dysbiosis and GS formation,and analyze bile microbiota composition in GS patients.METHODS This is a retrospective analysis conducted at Jinshan Hospital between 2022 and 2023.A total of 40 patients were analyzed,including 25 with GS and 15 with GSfree(GSF).Bile samples from 27 patients were analyzed using 16S rRNA gene sequencing to assess microbial composition.RESULTS Significant differences were found in bile acid profiles between GS and GSF groups,with lower microbial diversity in GS patients,indicated by reduced Shannon,Chao,Ace,and Sobs indices,and a higher Simpson index.At the phylum level,the most abundant taxa in GS patients were Proteobacteria(91.59%),Firmicutes(2.90%),and Actinobacteria(1.70%),while Proteobacteria(79.81%),Firmicutes(9.67%),and Bacteroidota(3.80%)were predominated in the GSF group.Notably,Achromobacter was more abundant in GS patients,while Acinetobacter,Lactobacillus,and Prevotella were enriched in the GSF group,suggesting their potential protective role against GS formation.CONCLUSION Dysbiosis,particularly overgrowth of Proteobacteria,may contribute to gallstone formation,while Lactobacillus could play a protective role.Further research is needed to validate these findings.展开更多
BACKGROUND This study aimed to identify characteristic gut genera in obese and normal-weight children(8-12 years old)using 16S rDNA sequencing.The research aimed to provide insights for mechanistic studies and prevent...BACKGROUND This study aimed to identify characteristic gut genera in obese and normal-weight children(8-12 years old)using 16S rDNA sequencing.The research aimed to provide insights for mechanistic studies and prevention strategies for childhood obesity.Thirty normal-weight and thirty age-and sex-matched obese children were included.Questionnaires and body measurements were collected,and fecal samples underwent 16S rDNA sequencing.Significant differences in body mass index(BMI)and body-fat percentage were observed between the groups.Analysis of gut microbiota diversity revealed lowerα-diversity in obese children.Differences in gut microbiota composition were found between the two groups.Prevotella and Firmicutes were more abundant in the obese group,while Bacteroides and Sanguibacteroides were more prevalent in the control group.AIM To identify the characteristic gut genera in obese and normal-weight children(8-12-year-old)using 16S rDNA sequencing,and provide a basis for subsequent mechanistic studies and prevention strategies for childhood obesity.METHODS Thirty each normal-weight,1:1 matched for age and sex,and obese children,with an obese status from 2020 to 2022,were included in the control and obese groups,respectively.Basic information was collected through questionnaires and body measurements were obtained from both obese and normal-weight children.Fecal samples were collected from both groups and subjected to 16S rDNA sequencing using an Illumina MiSeq sequencing platform for gut microbiota diversity analysis.RESULTS Significant differences in BMI and body-fat percentage were observed between the two groups.The Ace and Chao1 indices were significantly lower in the obese group than those in the control group,whereas differences were not significant in the Shannon and Simpson indices.Kruskal-Wallis tests indicated significant differences in unweighted and weighted UniFrac distances between the gut microbiota of normal-weight and obese children(P<0.01),suggesting substantial disparities in both the species and quantity of gut microbiota between the two groups.Prevotella,Firmicutes,Bacteroides,and Sanguibacteroides were more abundant in the obese and control groups,respectively.Heatmap results demonstrated significant differences in the gut microbiota composition between obese and normal-weight children.CONCLUSION Obese children exhibited lowerα-diversity in their gut microbiota than did the normal-weight children.Significant differences were observed in the composition of gut microbiota between obese and normal-weight children.展开更多
基金Supported by the Youth Scientific Research Launch Fund Project of Jinshan Hospital,Affiliated with Fudan University,No.JYQN-LC-202214.
文摘BACKGROUND Gallstone(GS),a prevalent biliary disorder,is associated with bile stasis,infection,and cholesterol metabolism.Recent research highlights the potential role of bile microbiota in GS pathogenesis.This is a case control study conducted at Jinshan Hospital between 2022 and 2023.AIM To investigate the relationship between bile microbiota dysbiosis and GS formation,and analyze bile microbiota composition in GS patients.METHODS This is a retrospective analysis conducted at Jinshan Hospital between 2022 and 2023.A total of 40 patients were analyzed,including 25 with GS and 15 with GSfree(GSF).Bile samples from 27 patients were analyzed using 16S rRNA gene sequencing to assess microbial composition.RESULTS Significant differences were found in bile acid profiles between GS and GSF groups,with lower microbial diversity in GS patients,indicated by reduced Shannon,Chao,Ace,and Sobs indices,and a higher Simpson index.At the phylum level,the most abundant taxa in GS patients were Proteobacteria(91.59%),Firmicutes(2.90%),and Actinobacteria(1.70%),while Proteobacteria(79.81%),Firmicutes(9.67%),and Bacteroidota(3.80%)were predominated in the GSF group.Notably,Achromobacter was more abundant in GS patients,while Acinetobacter,Lactobacillus,and Prevotella were enriched in the GSF group,suggesting their potential protective role against GS formation.CONCLUSION Dysbiosis,particularly overgrowth of Proteobacteria,may contribute to gallstone formation,while Lactobacillus could play a protective role.Further research is needed to validate these findings.
文摘BACKGROUND This study aimed to identify characteristic gut genera in obese and normal-weight children(8-12 years old)using 16S rDNA sequencing.The research aimed to provide insights for mechanistic studies and prevention strategies for childhood obesity.Thirty normal-weight and thirty age-and sex-matched obese children were included.Questionnaires and body measurements were collected,and fecal samples underwent 16S rDNA sequencing.Significant differences in body mass index(BMI)and body-fat percentage were observed between the groups.Analysis of gut microbiota diversity revealed lowerα-diversity in obese children.Differences in gut microbiota composition were found between the two groups.Prevotella and Firmicutes were more abundant in the obese group,while Bacteroides and Sanguibacteroides were more prevalent in the control group.AIM To identify the characteristic gut genera in obese and normal-weight children(8-12-year-old)using 16S rDNA sequencing,and provide a basis for subsequent mechanistic studies and prevention strategies for childhood obesity.METHODS Thirty each normal-weight,1:1 matched for age and sex,and obese children,with an obese status from 2020 to 2022,were included in the control and obese groups,respectively.Basic information was collected through questionnaires and body measurements were obtained from both obese and normal-weight children.Fecal samples were collected from both groups and subjected to 16S rDNA sequencing using an Illumina MiSeq sequencing platform for gut microbiota diversity analysis.RESULTS Significant differences in BMI and body-fat percentage were observed between the two groups.The Ace and Chao1 indices were significantly lower in the obese group than those in the control group,whereas differences were not significant in the Shannon and Simpson indices.Kruskal-Wallis tests indicated significant differences in unweighted and weighted UniFrac distances between the gut microbiota of normal-weight and obese children(P<0.01),suggesting substantial disparities in both the species and quantity of gut microbiota between the two groups.Prevotella,Firmicutes,Bacteroides,and Sanguibacteroides were more abundant in the obese and control groups,respectively.Heatmap results demonstrated significant differences in the gut microbiota composition between obese and normal-weight children.CONCLUSION Obese children exhibited lowerα-diversity in their gut microbiota than did the normal-weight children.Significant differences were observed in the composition of gut microbiota between obese and normal-weight children.
