AIM:To evaluate the impact of mass vaccination against the hepatitis B virus (HBV) in Egypt,and to search for vaccinee asymptomatic breakthrough HBV infection and its genotype.METHODS:Seven hundred serum samples from ...AIM:To evaluate the impact of mass vaccination against the hepatitis B virus (HBV) in Egypt,and to search for vaccinee asymptomatic breakthrough HBV infection and its genotype.METHODS:Seven hundred serum samples from vaccinated children and adults (aged 2-47 years) were used for quantitative and qualitative detection of HBsAb by ELISA.Three hundred and sixty serum samples representing undetectable or low or high HBsAb were screened for markers of active HBV infection (HBsAg,HBcAb (IgG) and HBeAb by ELISA,plus HBsAg by AxSYM) and HBV-DNA genotyping by nested multiplex PCR and by DNA sequencing.RESULTS:It was found that 65% of children aged 2-4 years,and 20.5% aged 4-13 years,as well as 45% adults were good responders to HBV vaccination mounting protective level HBsAb.Poor responders were 28%,59.5% and 34%,and non-responders were 7%,20% and 21% respectively,in the three studied groups.Markers of asymptomatic HBV infections were HBsAg detected by ELISA in 2.5% vs 11.39% by AxSYM.Other markers were HBcAb (IgG) in 1.38%,HBeAb in 0.83%,and HBV-DNA in 7.8%.All had HBV genotype E infection.CONCLUSION:It is concluded that HBV vaccine is efficient in controlling HBV infection among children and adults.The vaccine breakthrough infection was by HBV genotype E.A booster dose of vaccine is recommended,probably four years after initial vaccination.展开更多
Hepatitis B virus(HBV)(sub)genotypes A1,D3 and E circulate in sub-Saharan Africa,the region with one of the highest incidences of HBV-associated hepatocellular carcinoma globally.Although genotype E was identified mor...Hepatitis B virus(HBV)(sub)genotypes A1,D3 and E circulate in sub-Saharan Africa,the region with one of the highest incidences of HBV-associated hepatocellular carcinoma globally.Although genotype E was identified more than 20 years ago,and is the most widespread genotype in Africa,it has not been extensively studied.The current knowledge status and gaps in its origin and evolution,natural history of infection,disease progression,response to antiviral therapy and vaccination are discussed.Genotype E is an African genotype,with unique molecular characteristics that is found mainly in Western and Central Africa and rarely outside Africa except in individuals of African descent.The low prevalence of this genotype in the African descendant populations in the New World,phylogeographic analyses,the low genetic diversity and evidence of remnants of genotype E in ancient HBV samples suggests the relatively recent reintroduction into the population.There is scarcity of information on the clinical and virological characteristics of genotype E-infected patients,disease progression and outcomes and efficacy of anti-HBV drugs.Individuals infected with genotype E have been characterised with high hepatitis B e antigen-positivity and high viral load with a lower end of treatment response to interferon-alpha.A minority of genotype E-infected participants have been included in studies in which treatment response was monitored.Of concern is that current guidelines do not consider patients infected with genotype E.Thus,there is an urgent need for further large-scale investigations into genotype E,the neglected genotype of HBV.展开更多
Hepatitis E virus(HEV)infection is a common public health problem in developing countries.However,the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farm...Hepatitis E virus(HEV)infection is a common public health problem in developing countries.However,the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farming areas in central China are still inadequately understood.Here,cross-sectional serological and genotypic surveys of HEV among the 1232 general population,273 workers occupationally exposed to swine,and 276 pigs in a high-density pig-breeding area,were undertaken by ELISA and nested RT-PCR methods.Anti-HEV IgG was detected in 26.22%of general population and 48.35%of occupational workers.The prevalence of swine serum HEV-Ag was 6.52%.The prevalence of anti-HEV IgG was significantly higher among the workers occupationally exposed to swine than among the general population.An increased HEV seropositivity risk among the general population was associated with either being a peasant or male and was very strongly associated with the increase of age.Among the occupationally exposed group,the prevalence of anti-HEV IgG antibodies increased with age and working years.Among the 30 HEV-IgM-positive people,the infection rates of clerks in the public,peasants,pork retailers,and pig farmers were higher than those of others.A phylogenetic analysis revealed that all the isolates belonged to subgenotype 4d,and four people and four pigs shared 97.04%-100%sequence homology.This study revealed a high HEV seroprevalence among the general population and workers occupationally exposed to swine in the Anlu City,and supports the notion that swine are a source of human HEV infection.展开更多
Sorghum [<i><span style="font-family:Verdana;">Sorghum bicolor</span></i><span style="font-family:Verdana;"> (L.) Moench] is a high-yielding, nutrient-use efficient, a...Sorghum [<i><span style="font-family:Verdana;">Sorghum bicolor</span></i><span style="font-family:Verdana;"> (L.) Moench] is a high-yielding, nutrient-use efficient, and drought tolerant crop that can be cultivated on over 80 per cent of the world’s agricultural land. However, a number of biotic and abiotic factors are limiting grain yield increase. Diseases (leaf and grain) are considered as one of the major biotic factors hindering sorghum productivity in the highland and intermediate altitude sorghum growing areas of Ethiopia. In addition, the yield performance of crop varieties is highly influenced by genotype × environment (G × E) interaction which is the major focus of researchers while generating improved varieties. In Ethiopia, high yielding and stable varieties that withstand biotic stress in the highland areas are limited. In line with this, the yield performance of 21 sorghum genotypes and one standard check were evaluated across 14 environments with the objectives of estimating magnitude G </span><span style="font-family:Verdana;">× E interaction for grain yield and to identify high yielder and stable genotypes across environments. The experiment was laid out using Randomized Complete Block Design with three replications in all environments. The combined analysis of variance across environments revealed highly significant differences among environments, genotypes and G × E interactions of grain yield suggesting further analysis of the G × E interaction. The results of the combined AMMI analysis of variance indicated that the total variation in grain yield was attributed to environments effects 71.21%, genotypes effects 4.52% and G × E interactions effects 24.27% indicating the major sources of variation. Genotypes 2006AN7010 and 2006AN7011 were high yielder and they were stable across environments and one variety has been released for commercial production and can be used as parental lines for genetic improvement in the sorghum improvement program. In general, this research study revealed the importance of evaluating sorghum genotypes for their yield and stability across diverse highland areas of Ethiopia before releasing for commercial production.</span>展开更多
基金Biotechnology and Genetic Engineering National Strategy- Academy for Science,Research and Biotechnology for their financial support to this project
文摘AIM:To evaluate the impact of mass vaccination against the hepatitis B virus (HBV) in Egypt,and to search for vaccinee asymptomatic breakthrough HBV infection and its genotype.METHODS:Seven hundred serum samples from vaccinated children and adults (aged 2-47 years) were used for quantitative and qualitative detection of HBsAb by ELISA.Three hundred and sixty serum samples representing undetectable or low or high HBsAb were screened for markers of active HBV infection (HBsAg,HBcAb (IgG) and HBeAb by ELISA,plus HBsAg by AxSYM) and HBV-DNA genotyping by nested multiplex PCR and by DNA sequencing.RESULTS:It was found that 65% of children aged 2-4 years,and 20.5% aged 4-13 years,as well as 45% adults were good responders to HBV vaccination mounting protective level HBsAb.Poor responders were 28%,59.5% and 34%,and non-responders were 7%,20% and 21% respectively,in the three studied groups.Markers of asymptomatic HBV infections were HBsAg detected by ELISA in 2.5% vs 11.39% by AxSYM.Other markers were HBcAb (IgG) in 1.38%,HBeAb in 0.83%,and HBV-DNA in 7.8%.All had HBV genotype E infection.CONCLUSION:It is concluded that HBV vaccine is efficient in controlling HBV infection among children and adults.The vaccine breakthrough infection was by HBV genotype E.A booster dose of vaccine is recommended,probably four years after initial vaccination.
基金Supported by National Research Foundation(NRF)of South Africa,No.GUN#93516Deutsche Forschungsgemeinschaft(DFG,English:German Research Foundation)Cancer Association of South Africa(CANSA).
文摘Hepatitis B virus(HBV)(sub)genotypes A1,D3 and E circulate in sub-Saharan Africa,the region with one of the highest incidences of HBV-associated hepatocellular carcinoma globally.Although genotype E was identified more than 20 years ago,and is the most widespread genotype in Africa,it has not been extensively studied.The current knowledge status and gaps in its origin and evolution,natural history of infection,disease progression,response to antiviral therapy and vaccination are discussed.Genotype E is an African genotype,with unique molecular characteristics that is found mainly in Western and Central Africa and rarely outside Africa except in individuals of African descent.The low prevalence of this genotype in the African descendant populations in the New World,phylogeographic analyses,the low genetic diversity and evidence of remnants of genotype E in ancient HBV samples suggests the relatively recent reintroduction into the population.There is scarcity of information on the clinical and virological characteristics of genotype E-infected patients,disease progression and outcomes and efficacy of anti-HBV drugs.Individuals infected with genotype E have been characterised with high hepatitis B e antigen-positivity and high viral load with a lower end of treatment response to interferon-alpha.A minority of genotype E-infected participants have been included in studies in which treatment response was monitored.Of concern is that current guidelines do not consider patients infected with genotype E.Thus,there is an urgent need for further large-scale investigations into genotype E,the neglected genotype of HBV.
基金partly supported by General Projects of Health and Family Planning Commission of Hubei Province of China no. WJ2017M174, and WJ2017M240Occupational Hazard and Identification Control of Hubei Provincial Key Laboratory Open Fund, no. OCHI2017G02
文摘Hepatitis E virus(HEV)infection is a common public health problem in developing countries.However,the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farming areas in central China are still inadequately understood.Here,cross-sectional serological and genotypic surveys of HEV among the 1232 general population,273 workers occupationally exposed to swine,and 276 pigs in a high-density pig-breeding area,were undertaken by ELISA and nested RT-PCR methods.Anti-HEV IgG was detected in 26.22%of general population and 48.35%of occupational workers.The prevalence of swine serum HEV-Ag was 6.52%.The prevalence of anti-HEV IgG was significantly higher among the workers occupationally exposed to swine than among the general population.An increased HEV seropositivity risk among the general population was associated with either being a peasant or male and was very strongly associated with the increase of age.Among the occupationally exposed group,the prevalence of anti-HEV IgG antibodies increased with age and working years.Among the 30 HEV-IgM-positive people,the infection rates of clerks in the public,peasants,pork retailers,and pig farmers were higher than those of others.A phylogenetic analysis revealed that all the isolates belonged to subgenotype 4d,and four people and four pigs shared 97.04%-100%sequence homology.This study revealed a high HEV seroprevalence among the general population and workers occupationally exposed to swine in the Anlu City,and supports the notion that swine are a source of human HEV infection.
文摘Sorghum [<i><span style="font-family:Verdana;">Sorghum bicolor</span></i><span style="font-family:Verdana;"> (L.) Moench] is a high-yielding, nutrient-use efficient, and drought tolerant crop that can be cultivated on over 80 per cent of the world’s agricultural land. However, a number of biotic and abiotic factors are limiting grain yield increase. Diseases (leaf and grain) are considered as one of the major biotic factors hindering sorghum productivity in the highland and intermediate altitude sorghum growing areas of Ethiopia. In addition, the yield performance of crop varieties is highly influenced by genotype × environment (G × E) interaction which is the major focus of researchers while generating improved varieties. In Ethiopia, high yielding and stable varieties that withstand biotic stress in the highland areas are limited. In line with this, the yield performance of 21 sorghum genotypes and one standard check were evaluated across 14 environments with the objectives of estimating magnitude G </span><span style="font-family:Verdana;">× E interaction for grain yield and to identify high yielder and stable genotypes across environments. The experiment was laid out using Randomized Complete Block Design with three replications in all environments. The combined analysis of variance across environments revealed highly significant differences among environments, genotypes and G × E interactions of grain yield suggesting further analysis of the G × E interaction. The results of the combined AMMI analysis of variance indicated that the total variation in grain yield was attributed to environments effects 71.21%, genotypes effects 4.52% and G × E interactions effects 24.27% indicating the major sources of variation. Genotypes 2006AN7010 and 2006AN7011 were high yielder and they were stable across environments and one variety has been released for commercial production and can be used as parental lines for genetic improvement in the sorghum improvement program. In general, this research study revealed the importance of evaluating sorghum genotypes for their yield and stability across diverse highland areas of Ethiopia before releasing for commercial production.</span>
