TheTragopogon dubius is traditionally used to treatmany ailments,consumed as a vegetable,and utilized as fodder for livestock.Tragopogon dubius,found in the Kashmir Himalayas,is the least explored for its bioactivity ...TheTragopogon dubius is traditionally used to treatmany ailments,consumed as a vegetable,and utilized as fodder for livestock.Tragopogon dubius,found in the Kashmir Himalayas,is the least explored for its bioactivity properties and has a unique geographical location.This study is the first attempt to investigate the antioxidant,anticancer,and genoprotective properties of the aqueous extracts from the leaves(AQ-TrDL)and stems(AQ-TrDS)of this plant.AQ-TrDL and AQ-TrDS demonstrated significant amounts of phenolic and flavonoid contents.GC-HRMS identified various phytochemicals belonging to different classes,like carboxylic acids,fatty acid derivatives,phenols,and triterpenoids.DPPH,Superoxide,FRAP,and ABTS antioxidant assays showed that AQ-TrDS exhibited stronger radical scavenging activities than AQ-TrDL,with IC_(50)values ranging from 40.31 to 73.58μg/mL.Cytotoxicity tests revealed that AQ-TrDS significantly inhibited the growth of cancer cells inMCF-7,HCT-116,HeLa,and A-549 cell lines,with over 50%inhibition observed at concentrations ranging from 56.62 to 98.32μg/mL.Importantly,minimal effects were seen in normal fibroblast L-929 cells,with GI50 values over 434μg/mL.Additionally,genoprotective tests showed that AQ-TrDS effectively reduced“H_2)O_(2)”-induced DNA damage in lymphocytes,decreasing damage by up to 61.18%at a concentration of 320μg/mL.HPLC analysis of amino acids identified 10 amino acids in T.dubius leaves and 14 in stems,showing its nutritional value.Overall,these findings highlight the biomedical potential of T.dubius aqueous extracts for developing new pharmaceutical agents.展开更多
Objective: To evidence the ability of ethanol fruit extract from Detarium microcarpum(D. microcarpum) to preserve DNA integrity against oxidative genomic damage.Methods: Ethanol extract from D. microcarpum fruit pulp ...Objective: To evidence the ability of ethanol fruit extract from Detarium microcarpum(D. microcarpum) to preserve DNA integrity against oxidative genomic damage.Methods: Ethanol extract from D. microcarpum fruit pulp was analyzed for its antioxidant capacity using ferric reducing antioxidant power, 2,2-diphenyl-1-picrylhydrazyl,2,20-azinobis-3-ethyl-ethylbenzothiazoline-6-sulphonate, superoxide anion, deoxyribose degradation and lipid peroxidation models. The genoprotective activity was assessed ex vivo by comet assay, on liver cells of NMRI female mice using cyclophosphamide(CP) as genotoxic agent.Results: Ethanol extract from D. microcarpum fruit pulp exhibited interesting antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl, deoxyribose degradation and lipid peroxidation assays. The extract did not present any genotoxic effect but protected DNA against CP-induced damages with a dose-dependent manner. The genoprotective effect observed was related to the antioxidant molecules of the fruit that scavenged the hydroxyl radical(generated by the metabolism of CP) as well as the peroxyl and alkoxyl radicals issued from lipid peroxidation. Other mechanisms such as inactivation of CP metabolism to genotoxic end products, induction of the expression of antioxidant and DNA repair enzymes have been discussed.Conclusions: Our results suggest that the wild edible fruit from D. microcarpum could be beneficial on consumer's health by its antioxidant and genoprotective effects, particularly during chemotherapies exhibiting genotoxic effects like CP in cancer treatment.展开更多
基金supported by Research institute/Center Supporting Program(RICSP-25-3),King Saud University,Riyadh,Saudi Arabia.
文摘TheTragopogon dubius is traditionally used to treatmany ailments,consumed as a vegetable,and utilized as fodder for livestock.Tragopogon dubius,found in the Kashmir Himalayas,is the least explored for its bioactivity properties and has a unique geographical location.This study is the first attempt to investigate the antioxidant,anticancer,and genoprotective properties of the aqueous extracts from the leaves(AQ-TrDL)and stems(AQ-TrDS)of this plant.AQ-TrDL and AQ-TrDS demonstrated significant amounts of phenolic and flavonoid contents.GC-HRMS identified various phytochemicals belonging to different classes,like carboxylic acids,fatty acid derivatives,phenols,and triterpenoids.DPPH,Superoxide,FRAP,and ABTS antioxidant assays showed that AQ-TrDS exhibited stronger radical scavenging activities than AQ-TrDL,with IC_(50)values ranging from 40.31 to 73.58μg/mL.Cytotoxicity tests revealed that AQ-TrDS significantly inhibited the growth of cancer cells inMCF-7,HCT-116,HeLa,and A-549 cell lines,with over 50%inhibition observed at concentrations ranging from 56.62 to 98.32μg/mL.Importantly,minimal effects were seen in normal fibroblast L-929 cells,with GI50 values over 434μg/mL.Additionally,genoprotective tests showed that AQ-TrDS effectively reduced“H_2)O_(2)”-induced DNA damage in lymphocytes,decreasing damage by up to 61.18%at a concentration of 320μg/mL.HPLC analysis of amino acids identified 10 amino acids in T.dubius leaves and 14 in stems,showing its nutritional value.Overall,these findings highlight the biomedical potential of T.dubius aqueous extracts for developing new pharmaceutical agents.
基金Supported by the International Foundation for Science(IFS)Stockholm under the IFS Grants F/5539-1 and F/4445-1+1 种基金the World Academy of Science(TWAS)Trieste under the grant TWAS 12-044 RG/BIO/AF/AC_G
文摘Objective: To evidence the ability of ethanol fruit extract from Detarium microcarpum(D. microcarpum) to preserve DNA integrity against oxidative genomic damage.Methods: Ethanol extract from D. microcarpum fruit pulp was analyzed for its antioxidant capacity using ferric reducing antioxidant power, 2,2-diphenyl-1-picrylhydrazyl,2,20-azinobis-3-ethyl-ethylbenzothiazoline-6-sulphonate, superoxide anion, deoxyribose degradation and lipid peroxidation models. The genoprotective activity was assessed ex vivo by comet assay, on liver cells of NMRI female mice using cyclophosphamide(CP) as genotoxic agent.Results: Ethanol extract from D. microcarpum fruit pulp exhibited interesting antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl, deoxyribose degradation and lipid peroxidation assays. The extract did not present any genotoxic effect but protected DNA against CP-induced damages with a dose-dependent manner. The genoprotective effect observed was related to the antioxidant molecules of the fruit that scavenged the hydroxyl radical(generated by the metabolism of CP) as well as the peroxyl and alkoxyl radicals issued from lipid peroxidation. Other mechanisms such as inactivation of CP metabolism to genotoxic end products, induction of the expression of antioxidant and DNA repair enzymes have been discussed.Conclusions: Our results suggest that the wild edible fruit from D. microcarpum could be beneficial on consumer's health by its antioxidant and genoprotective effects, particularly during chemotherapies exhibiting genotoxic effects like CP in cancer treatment.
