Common wheat （Triticum aestivum L.） is one of the most important crops, and intra-specific wheat hybrids have obvious heterosis in yield and protein quality. Therefore, utilization of hybrid wheat varieties offers a...Common wheat （Triticum aestivum L.） is one of the most important crops, and intra-specific wheat hybrids have obvious heterosis in yield and protein quality. Therefore, utilization of hybrid wheat varieties offers an effective way to increase yield and nutrition. Cytoplasmic male sterility （CMS） systems are a useful genetic tool for hybrid crop breeding, and are ideal models for studying the genetic interaction and cooperative function of mitochondrial and nuclear genomes in plants （Schnable and Wise, 1998; Hanson and Bentolila, 2004）.展开更多
Objective:In this study,we aimed to identify novel genetic loci and protein biomarkers associated with silicosis susceptibility in Chinese workers through integrated proteomic and genomic analyses and to develop an ea...Objective:In this study,we aimed to identify novel genetic loci and protein biomarkers associated with silicosis susceptibility in Chinese workers through integrated proteomic and genomic analyses and to develop an early diagnostic prediction model.Methods:A genome-wide association study(GWAS)was conducted on 163 patients with silicosis and 183 controls,followed by Olink proteomic profiling of 92 plasma proteins.Protein quantitative trait loci(pQTL)mapping,Mendelian randomization(MR),and Bayesian co-localization were used to infer causal relationships.A causal protein risk score(CPRS)model integrating genetic and proteomic data was developed and validated using 10-fold cross-validation.Results:GWAS identified 16 novel risk loci(P<1×10^(-5)),including rs6677666(WLS)and rs2272528(COL4A4).MR analysis revealed eight plasma proteins associated with silicosis risk,with MMP12,EGF,Gal_9,GZMA,and ICOSLG showing significant differential expression(P<0.05).The CPRS model combining these proteins demonstrated a high diagnostic accuracy(AUC=0.915),outperforming traditional clinical variables.Conclusion:This multi-omics study uncovered genetic and proteomic markers linked to silicosis susceptibility and established a robust predictive model.The integration of GWAS and proteomics offers novel insights into the pathogenesis of silicosis,and supports development of early detection and prevention policies for high-risk populations.展开更多
On May 1,2025,Gonzalez-Perez and colleagues(1)reported a multi-country outbreak caused by Salmonella enterica subspecies enterica serovar Mbandaka(S.Mbandaka)that resulted in over 200 cases across Europe.Finland repor...On May 1,2025,Gonzalez-Perez and colleagues(1)reported a multi-country outbreak caused by Salmonella enterica subspecies enterica serovar Mbandaka(S.Mbandaka)that resulted in over 200 cases across Europe.Finland reported the highest number of cases with 97 infections.Whole genome sequencing(WGS)and comparative genomic analyses revealed that the outbreak strains were genetically linked to previously identified strains in the United Kingdom and to pre-cooked,frozen chicken meat used in ready-to-eat products.Subsequently,on May 5,2025,the United States of America(USA)Centers for Disease Control and Prevention(CDC)announced a Salmonella outbreak associated with backyard poultry(2).As of May 19,2025,this outbreak had expanded to 104 confirmed infections,with at least 33 individuals contracting Salmonella following contact with backyard poultry.Tragically,one death has been reported in Illinois.Scientific evidence demonstrates that S.Mbandaka isolated from patient samples,and matched the strain found in shipping boxes used to transport poultry from hatcheries to agricultural retail stores.展开更多
Rapid advances in sequencing and bioinformatics have profoundly enhanced disease understanding[1],while integrated genomic analyses reveal regulatory networks governing biological processes[2].However,research remains...Rapid advances in sequencing and bioinformatics have profoundly enhanced disease understanding[1],while integrated genomic analyses reveal regulatory networks governing biological processes[2].However,research remains disproportionately focused on populations of European ancestry or other groups with large sample sizes,such as Han Chinese,leaving many ethnically diverse and underrepresented populations disadvantaged in genomic medicine[3].Complex human demographic processes influence allele frequencies,define gene sets under selective constraints,and inform variant-filtering strategies crucial for genetic disease diagnosis and genetic counseling.This imbalance limits the global applicability of genomic findings and exacerbates existing health disparities.By prioritizing high-quality genome sequencing from underrepresented populations,researchers can uncover novel genetic variants,refine disease risk predictions,and promote equitable access to precision medicine.展开更多
基金supported by the National Natural Science Foundation of China(No.30971844)the Fundamental Research Funds of Northwest A & F University(No. QN2011003)+1 种基金China Postdoctoral Science Foundation to Wang Junwei(No.20070410835)the Tang Zhong-Ying Breeding Funding Project of Northwest A & F University
文摘Common wheat （Triticum aestivum L.） is one of the most important crops, and intra-specific wheat hybrids have obvious heterosis in yield and protein quality. Therefore, utilization of hybrid wheat varieties offers an effective way to increase yield and nutrition. Cytoplasmic male sterility （CMS） systems are a useful genetic tool for hybrid crop breeding, and are ideal models for studying the genetic interaction and cooperative function of mitochondrial and nuclear genomes in plants （Schnable and Wise, 1998; Hanson and Bentolila, 2004）.
基金Supported by the Jiangsu Provincial Social Development Program of the Key R&D Project(BE2022803)Natural Science Foundation of Jiangsu(BK20201485)+1 种基金Jiangsu Provincial Key Medical Discipline(ZDXK202249)Scientific Research Project of Jiangsu Health Commission(M2022085).
文摘Objective:In this study,we aimed to identify novel genetic loci and protein biomarkers associated with silicosis susceptibility in Chinese workers through integrated proteomic and genomic analyses and to develop an early diagnostic prediction model.Methods:A genome-wide association study(GWAS)was conducted on 163 patients with silicosis and 183 controls,followed by Olink proteomic profiling of 92 plasma proteins.Protein quantitative trait loci(pQTL)mapping,Mendelian randomization(MR),and Bayesian co-localization were used to infer causal relationships.A causal protein risk score(CPRS)model integrating genetic and proteomic data was developed and validated using 10-fold cross-validation.Results:GWAS identified 16 novel risk loci(P<1×10^(-5)),including rs6677666(WLS)and rs2272528(COL4A4).MR analysis revealed eight plasma proteins associated with silicosis risk,with MMP12,EGF,Gal_9,GZMA,and ICOSLG showing significant differential expression(P<0.05).The CPRS model combining these proteins demonstrated a high diagnostic accuracy(AUC=0.915),outperforming traditional clinical variables.Conclusion:This multi-omics study uncovered genetic and proteomic markers linked to silicosis susceptibility and established a robust predictive model.The integration of GWAS and proteomics offers novel insights into the pathogenesis of silicosis,and supports development of early detection and prevention policies for high-risk populations.
基金Supported in part by grants from the Project for the Young Scientist of the Joint Funds of Science and Technology Research and Development Plan of Henan Province,China(235200810058)the National Key Research and Development Program of China(2023YFC2307101)the Young Top-Notch Talents Foundation of Henan Agricultural University(30501278).
文摘On May 1,2025,Gonzalez-Perez and colleagues(1)reported a multi-country outbreak caused by Salmonella enterica subspecies enterica serovar Mbandaka(S.Mbandaka)that resulted in over 200 cases across Europe.Finland reported the highest number of cases with 97 infections.Whole genome sequencing(WGS)and comparative genomic analyses revealed that the outbreak strains were genetically linked to previously identified strains in the United Kingdom and to pre-cooked,frozen chicken meat used in ready-to-eat products.Subsequently,on May 5,2025,the United States of America(USA)Centers for Disease Control and Prevention(CDC)announced a Salmonella outbreak associated with backyard poultry(2).As of May 19,2025,this outbreak had expanded to 104 confirmed infections,with at least 33 individuals contracting Salmonella following contact with backyard poultry.Tragically,one death has been reported in Illinois.Scientific evidence demonstrates that S.Mbandaka isolated from patient samples,and matched the strain found in shipping boxes used to transport poultry from hatcheries to agricultural retail stores.
基金supported by the Sichuan Science and Technology Program(grant number 2024NSFSC1518 to G.H.)the National Natural Science Foundation of China(grant number 82402203 to G.H.)+3 种基金the 1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(grant number ZYJC20002 to H.Y.)the Major Project of the National Social Science Foundation of China(grant number 23&ZD203 to G.H.)the Open Project of the Key Laboratory of Forensic Genetics of the Ministry of Public Security(grant numbers 2022FGKFKT05 to G.H.and 2024FGKFKT02 to M.W.)the Center for Archaeological Science of Sichuan University(grant numbers 23SASA01 and 24SASB03 to G.H.).
文摘Rapid advances in sequencing and bioinformatics have profoundly enhanced disease understanding[1],while integrated genomic analyses reveal regulatory networks governing biological processes[2].However,research remains disproportionately focused on populations of European ancestry or other groups with large sample sizes,such as Han Chinese,leaving many ethnically diverse and underrepresented populations disadvantaged in genomic medicine[3].Complex human demographic processes influence allele frequencies,define gene sets under selective constraints,and inform variant-filtering strategies crucial for genetic disease diagnosis and genetic counseling.This imbalance limits the global applicability of genomic findings and exacerbates existing health disparities.By prioritizing high-quality genome sequencing from underrepresented populations,researchers can uncover novel genetic variants,refine disease risk predictions,and promote equitable access to precision medicine.
