Nitrate is the primary nitrogen source for plants and is a signaling molecule regulating various plant developmental processes. Despite its significance, limited information is available on nitrate signaling in Vitis ...Nitrate is the primary nitrogen source for plants and is a signaling molecule regulating various plant developmental processes. Despite its significance, limited information is available on nitrate signaling in Vitis vinifera. We identified nine Vv RWP-RK genes distributed across eight chromosomes using genome-wide identification and evolutionary analyses. Among these, Vv NLP1-4 and Vv RKD1-5 are associated with nitrate signaling and reproductive growth, respectively. To investigate their potential functions, structures, cis-acting promoter elements,functional structural domains, phylogenetic trees, spatiotemporal expression levels in different tissues at different developmental stages,potential protein-protein interaction networks, synteny(gene content), collinearity(gene order), and three-dimensional protein structure prediction were explored. We found that long-term nitrate application dramatically promoted grapevine plantlet development, including primary root length and leaf growth, and Vv NLP1.1, Vv NLP1.2, and Vv NLP2 were highly expressed in ‘Thompson Seedless' root tissues under nitrate-enriched conditions. To clarify the critical role of nitrate in grapevine growth, we observed that nuclear localization of Vv NLP1.1increased significantly following nitrate treatment. Vv NLP1.1 was found to bind to the promoter of the primary nitrate response gene Vv NRT1.1,driving its transcriptional activity. These findings indicate that Vv NLP1.1 is a core transcription factor of the nitrate signaling pathway in grapevine. Nitrate molecular docking analysis revealed that Vv NLP1.1 directly binds to nitrate ions, indicating its potential role as a nitrate sensor capable of directly perceiving nitrate concentration. We also discovered that short-term nitrate starvation impacts Vv NLP1.1 promoter activity, linked to the abscisic acid-binding element(ABRE) motif in its promoter region. Our results thus provide new insights into the molecular mechanisms underlying various physiological processes in grapevine, particularly the nitrate signaling pathway, and provide a theoretical basis for improving nitrogen use efficiency(NUE) in grapevine.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.32260727,32472670,and 32371924)the Natural Science Foundation of Ningxia(Grant Nos.2024AAC02039 and 2022AAC02024)+2 种基金the Key Research and Development Program of Xinjiang Uygur Autonomous Region(Grant No.2022B02034-3)the Key Research and Development Program of Ningxia Hui Autonomous Region(Grant No.2024BBF01003)the Science and Technology Plan Project of Xi'an City(Grant No.23NYGG0028).
文摘Nitrate is the primary nitrogen source for plants and is a signaling molecule regulating various plant developmental processes. Despite its significance, limited information is available on nitrate signaling in Vitis vinifera. We identified nine Vv RWP-RK genes distributed across eight chromosomes using genome-wide identification and evolutionary analyses. Among these, Vv NLP1-4 and Vv RKD1-5 are associated with nitrate signaling and reproductive growth, respectively. To investigate their potential functions, structures, cis-acting promoter elements,functional structural domains, phylogenetic trees, spatiotemporal expression levels in different tissues at different developmental stages,potential protein-protein interaction networks, synteny(gene content), collinearity(gene order), and three-dimensional protein structure prediction were explored. We found that long-term nitrate application dramatically promoted grapevine plantlet development, including primary root length and leaf growth, and Vv NLP1.1, Vv NLP1.2, and Vv NLP2 were highly expressed in ‘Thompson Seedless' root tissues under nitrate-enriched conditions. To clarify the critical role of nitrate in grapevine growth, we observed that nuclear localization of Vv NLP1.1increased significantly following nitrate treatment. Vv NLP1.1 was found to bind to the promoter of the primary nitrate response gene Vv NRT1.1,driving its transcriptional activity. These findings indicate that Vv NLP1.1 is a core transcription factor of the nitrate signaling pathway in grapevine. Nitrate molecular docking analysis revealed that Vv NLP1.1 directly binds to nitrate ions, indicating its potential role as a nitrate sensor capable of directly perceiving nitrate concentration. We also discovered that short-term nitrate starvation impacts Vv NLP1.1 promoter activity, linked to the abscisic acid-binding element(ABRE) motif in its promoter region. Our results thus provide new insights into the molecular mechanisms underlying various physiological processes in grapevine, particularly the nitrate signaling pathway, and provide a theoretical basis for improving nitrogen use efficiency(NUE) in grapevine.
