Tea,originating in China over 3,000 years ago,has transitioned from a medicinal herb to a widely consumed beverage.Despite considerable research focusing on tea plants in southwestern China,little attention has been p...Tea,originating in China over 3,000 years ago,has transitioned from a medicinal herb to a widely consumed beverage.Despite considerable research focusing on tea plants in southwestern China,little attention has been paid to those on Hainan Island.The notable resemblance between Hainan tea and C.sinensis var.assamica,alongside the unique geographical and climatic conditions of Hainan Island,has presented significant challenges for taxonomic and genetic investigations concerning Hainan tea.Our study bridged this gap by collecting 500 samples from Hainan Province and employing whole-genome resequencing to examine interspecific differences between Hainan tea and cultivated varieties.The findings confirmed the distinct taxonomic position of Hainan tea within Camellia sinensis,providing valuable insights for resource conservation and molecular breeding.Furthermore,our methodology offers a framework for investigating the origin,domestication,and genetic diversity of other species native to Hainan Island.展开更多
Limosilactobacillus reuteri is a resident probiotic in the intestines of animals with strong environmental adaptability.In the current study,to elucidate the adaptation strategies of L.reuteri to the intestine of mice...Limosilactobacillus reuteri is a resident probiotic in the intestines of animals with strong environmental adaptability.In the current study,to elucidate the adaptation strategies of L.reuteri to the intestine of mice supplemented with three dietary components(oil,peptide and cellulose),whole genome resequencing and determination of intracellular metabolites were carried out.The resequencing results showed that L.reuteri TO60(LR-TO)from the intestines of mice supplemented with high docosahexaenoic acid(DHA)tuna oil,or fermented Benincasa hispida culture(L.reuteri FB151,abbreviated as LR-FB)had relatively consistent single nucleotide polymorphisms(SNPs)and insertions and deletions(InDels),and those of L.reuteri from the intestines of mice supplemented with Rhopilema esculentum polypeptides(L.reuteri RE225,abbreviated as LR-RE)were quite different from them.The KEGG functional annotation of resequencing and the intracellular metabolites suggested that in all of the L.reuteri adapted to the intestinal environment mainly by regulating carbohydrate metabolism and membrane transport metabolic pathways.However,there were still some differences in their genes and metabolites,which may be related to the changes in the intestinal environment caused by three different types of dietary interventions.Our study analyzed the adaptation mechanism of L.reuteri to different isolated sources at the genetic level and metabolic level,which is helpful to clarify the adaptation strategies of probiotics to the intestinal environment,and provide supplementary information for the use of dietary nutritional supplements.展开更多
Dear Editor,Recently,Liang et al.(2024)conducted comprehensive genome resequencing and metabolomic analyses of 299 representative accessions from extant citrus species,offering an extensive overview of genetic diversi...Dear Editor,Recently,Liang et al.(2024)conducted comprehensive genome resequencing and metabolomic analyses of 299 representative accessions from extant citrus species,offering an extensive overview of genetic diversity and metabolic profiles across citrus populations.Their study included a correlation analysis that assessed the relationship between metabolite content and bioactivities,resulting in the selection of the top 100 annotated metabolites exhibiting the highest correlation coefficients(Pearson’s r)across various chemical assays,cell lines,and CYP450 enzymes(Liang et al.,2024).展开更多
基金supported by the Hainan Academy of Agricultural Sciences Research Project(HAAS2022KJCX03)Research and Demonstration on Key Technologies of Germplasm Resource Bank Construction and Resource Innovation Utilization of Wuzhishan Big Leaf Tea(ZDYF2024XDNY245)Monitoring and Analysis of Key Quality Components of Hainan Big Leaf Black Tea and Development and Demonstration of New Standardized Processing Technology(WZSKTPXM202202).
文摘Tea,originating in China over 3,000 years ago,has transitioned from a medicinal herb to a widely consumed beverage.Despite considerable research focusing on tea plants in southwestern China,little attention has been paid to those on Hainan Island.The notable resemblance between Hainan tea and C.sinensis var.assamica,alongside the unique geographical and climatic conditions of Hainan Island,has presented significant challenges for taxonomic and genetic investigations concerning Hainan tea.Our study bridged this gap by collecting 500 samples from Hainan Province and employing whole-genome resequencing to examine interspecific differences between Hainan tea and cultivated varieties.The findings confirmed the distinct taxonomic position of Hainan tea within Camellia sinensis,providing valuable insights for resource conservation and molecular breeding.Furthermore,our methodology offers a framework for investigating the origin,domestication,and genetic diversity of other species native to Hainan Island.
基金sponsored by the Natural Science Foundation of Zhejiang Province(LY19C010003)the Natural Science Foundation of Zhejiang Province(LQ22D060002)+1 种基金the Fund of State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products(ZS20190105)the K.C.Wong Magna Fund of Ningbo University.
文摘Limosilactobacillus reuteri is a resident probiotic in the intestines of animals with strong environmental adaptability.In the current study,to elucidate the adaptation strategies of L.reuteri to the intestine of mice supplemented with three dietary components(oil,peptide and cellulose),whole genome resequencing and determination of intracellular metabolites were carried out.The resequencing results showed that L.reuteri TO60(LR-TO)from the intestines of mice supplemented with high docosahexaenoic acid(DHA)tuna oil,or fermented Benincasa hispida culture(L.reuteri FB151,abbreviated as LR-FB)had relatively consistent single nucleotide polymorphisms(SNPs)and insertions and deletions(InDels),and those of L.reuteri from the intestines of mice supplemented with Rhopilema esculentum polypeptides(L.reuteri RE225,abbreviated as LR-RE)were quite different from them.The KEGG functional annotation of resequencing and the intracellular metabolites suggested that in all of the L.reuteri adapted to the intestinal environment mainly by regulating carbohydrate metabolism and membrane transport metabolic pathways.However,there were still some differences in their genes and metabolites,which may be related to the changes in the intestinal environment caused by three different types of dietary interventions.Our study analyzed the adaptation mechanism of L.reuteri to different isolated sources at the genetic level and metabolic level,which is helpful to clarify the adaptation strategies of probiotics to the intestinal environment,and provide supplementary information for the use of dietary nutritional supplements.
文摘Dear Editor,Recently,Liang et al.(2024)conducted comprehensive genome resequencing and metabolomic analyses of 299 representative accessions from extant citrus species,offering an extensive overview of genetic diversity and metabolic profiles across citrus populations.Their study included a correlation analysis that assessed the relationship between metabolite content and bioactivities,resulting in the selection of the top 100 annotated metabolites exhibiting the highest correlation coefficients(Pearson’s r)across various chemical assays,cell lines,and CYP450 enzymes(Liang et al.,2024).
