Chinese hamster with Chinese characteristics is used in experiments,and it is of great value in the field of medical biology research.However,at present,there is no high-efficiency method for evaluating the genetic qu...Chinese hamster with Chinese characteristics is used in experiments,and it is of great value in the field of medical biology research.However,at present,there is no high-efficiency method for evaluating the genetic quality of Chinese hamsters.Here,we developed a novel Chinese hamster genetic quality detection system using single-nucleotide polymorphism(SNP)markers.To find SNP loci,we conducted whole genome sequencing on 24 Chinese hamsters.Then,we employed an SNP locus screening criterion that we set up previously and initially screened 214 SNP loci with wide genome distribution and high polymorphism level.Subsequently,we developed the SNP detection system using a multitarget region capture technique based on second-generation sequencing,and a 55 SNP panel for genetic evaluation of Chinese hamster populations was developed.PopGen.32.analysis results showed that the average effective allele number,Shannon index,observed heterozygosity,expected heterozygosity,average heterozygosity,polymorphism information,and other genetic parameters of Chinese hamster population A were higher than those in population B.Using scientific screening and optimization,we successfully developed a novel Chinese hamster SNP genetic detection system that can efficiently and accurately analyze the genetic quality of the Chinese hamster population.展开更多
[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence,3'-junction and 5'-junction sequence between host plant D...[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence,3'-junction and 5'-junction sequence between host plant DNA integrated DNA of MON89788 soybean were amplified independently,and the three fragments were cloned into the cloning vector pMD18-T in order through molecular manipulation method to construct pMD-LM3M5,the applicability of the constructed novel PRM was tested. [Result] Sequencing confirmation result showed that the PRM was 3 700 bp in length,containing 1 029 bp of recombined DNA fragment. The limits of qualitative detection of the PRM were 10 copies. [Conclusion] The PRM constructed in this study was suitable for the identification of MON89788 event.展开更多
Perit on eal metastasis is the most comm on patter n of recurre nee and the most freque nt cause of death after surgery in patie nts with gastric can cer.Perit on eal free can cer cells dissem in ated from the primary...Perit on eal metastasis is the most comm on patter n of recurre nee and the most freque nt cause of death after surgery in patie nts with gastric can cer.Perit on eal free can cer cells dissem in ated from the primary lesi on site have bee n con sidered the main cause of perit on eal metastasis.Perit on eal lavage cytological exam in ati on(PLC)has bee n show n to be an in depe ndent predictor of gastric cancer relapse after curative resection and poor overall survival.However,the conventional cytological examinations have high rates of false-positive and false-negative findings.To improve the sensitivity,molecular-based methods using reverse transcriptase polymerase chain reaction have been developed for detecting cancer cells in peritoneal wash fluids of patients with gastric cancer.We performed a PubMed search for articles describing PLC in gastric can cer.Releva nt articles were reviewed and data on available outcomes elaborated.The cli nical roles and attributes of PLC in gastric can cer were reviewed,and its future applicati on to this disease is discussed.展开更多
Hot components operate in a high-temperature and high-pressure environment. The occurrence of a fault in hot components leads to high economic losses. In general, exhaust gas temperature(EGT) is used to monitor the pe...Hot components operate in a high-temperature and high-pressure environment. The occurrence of a fault in hot components leads to high economic losses. In general, exhaust gas temperature(EGT) is used to monitor the performance of hot components.However, during the early stages of a failure, the fault information is weak, and is simultaneously affected by various types of interference, such as the complex working conditions, ambient conditions, gradual performance degradation of the compressors and turbines, and noise. Additionally, inadequate effective information of the gas turbine also restricts the establishment of the detection model. To solve the above problems, this paper proposes an anomaly detection method based on frequent pattern extraction. A frequent pattern model(FPM) is applied to indicate the inherent regularity of change in EGT occurring from different types of interference. In this study, based on a genetic algorithm and support vector machine regression, the relationship model between the EGT and interference was tentatively built. The modeling accuracy was then further improved through the selection of the kernel function and training data. Experiments indicate that the optimal kernel function is linear and that the optimal training data should be balanced in addition to covering the appropriate range of operating conditions and ambient temperature. Furthermore, the thresholds based on the Pauta criterion that is automatically obtained during the modeling process, are used to determine whether hot components are operating abnormally. Moreover, the FPM is compared with the similarity theory, which demonstrates that the FPM can better suppress the effect of the component performance degradation and fuel heat value fluctuation. Finally, the effectiveness of the proposed method is validated on seven months of actual data obtained from a Titan130 gas turbine on an offshore oil platform. The results indicate that the proposed method can sensitively detect malfunctions in hot components during the early stages of a fault, and is robust to various types of interference.展开更多
Fluidized bioleaching is an efficient,environmentally friendly,and cost-effective mining method that has been widely explored and utilized for recovering low-grade copper sulfide minerals,such as chalcopyrite.However,...Fluidized bioleaching is an efficient,environmentally friendly,and cost-effective mining method that has been widely explored and utilized for recovering low-grade copper sulfide minerals,such as chalcopyrite.However,the proliferation and apoptosis of dominant leaching bacteria,such as Acidithiobacillus ferrooxidans,within complex pore,void,and fracture structures in deep-earth environments commonly results in a dynamic bacterial community that evolves continuously.This unclear genetic-scale microbial succession often leads to low leaching reaction efficiency,undesirable reaction passivation,and poor bioleaching operations.This review integrates genetic-scale insights with industrial challenges in chalcopyrite bioleaching,proposing novel strategies for regulating microbial communities.A systematic analysis of five critical dimensions is conducted,focusing on:1)The adaptations of Acidithiobacillus spp.to high Ag^(+) stress.2)The direct,indirect,and cooperative bioleaching pathways are linked to bacterial extracellular polymer substance(EPS)and Fe/S oxidation genes.3)The passivation dynamics governed by bacterial genomics,including thiosulfate,polysulfide,and biofilm mechanisms.4)The microbial succession patterns under genetic control Hi-C sequencing-guided consortia design.5)Molecular detection methods(16S rDNA,Hi-C)for optimizing leaching efficiency.The following innovations have been identified as being of key significance:A genomic-environmental interaction model has been developed to bridge the gap between bacterial genetics and passivation dynamics.A comprehensive analysis of Ag^(+) catalysis has been conducted,resulting in a 40%reduction in jarosite formation through jar gene suppression.Practical strategies,such as thermophilic consortia engineering,have been validated in pilot trials,achieving a 32%increase in copper recovery.Additionally,this study meticulously reviews and summarizes typical potential stimulations and enhanced bioleaching methods.The genetic sequencing methods,such as 16S rDNA and Hi-C,have been shown to hold promising potential for improving bioleaching reactions and delaying the formation of passivation substances like jarosite.展开更多
Objective To conduct clinical analysis and genetic variant detection in patients with congenital hypothyroidism,and to explore the role of paired box 8 (PAX8) gene variants in the pathogenic mechanism of congenital hy...Objective To conduct clinical analysis and genetic variant detection in patients with congenital hypothyroidism,and to explore the role of paired box 8 (PAX8) gene variants in the pathogenic mechanism of congenital hypothyroidism,thereby providing insights for its diagnosis and management.展开更多
基金National Key Research and Development Program for Young scientists,Grant/Award Number:2021YFF0703200National Natural Foundation Joint Fund for Regional Innovation and Development,Grant/Award Number:U21A20194+1 种基金National Natural Science Foundation of China,Grant/Award Number:32170540National Key Research and Development Program,Grant/Award Number:2022YFF0711005。
文摘Chinese hamster with Chinese characteristics is used in experiments,and it is of great value in the field of medical biology research.However,at present,there is no high-efficiency method for evaluating the genetic quality of Chinese hamsters.Here,we developed a novel Chinese hamster genetic quality detection system using single-nucleotide polymorphism(SNP)markers.To find SNP loci,we conducted whole genome sequencing on 24 Chinese hamsters.Then,we employed an SNP locus screening criterion that we set up previously and initially screened 214 SNP loci with wide genome distribution and high polymorphism level.Subsequently,we developed the SNP detection system using a multitarget region capture technique based on second-generation sequencing,and a 55 SNP panel for genetic evaluation of Chinese hamster populations was developed.PopGen.32.analysis results showed that the average effective allele number,Shannon index,observed heterozygosity,expected heterozygosity,average heterozygosity,polymorphism information,and other genetic parameters of Chinese hamster population A were higher than those in population B.Using scientific screening and optimization,we successfully developed a novel Chinese hamster SNP genetic detection system that can efficiently and accurately analyze the genetic quality of the Chinese hamster population.
基金Supported by Major Projects of Cultivating New Varieties by Trans-genic Technology (2008ZX08012-001)~~
文摘[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence,3'-junction and 5'-junction sequence between host plant DNA integrated DNA of MON89788 soybean were amplified independently,and the three fragments were cloned into the cloning vector pMD18-T in order through molecular manipulation method to construct pMD-LM3M5,the applicability of the constructed novel PRM was tested. [Result] Sequencing confirmation result showed that the PRM was 3 700 bp in length,containing 1 029 bp of recombined DNA fragment. The limits of qualitative detection of the PRM were 10 copies. [Conclusion] The PRM constructed in this study was suitable for the identification of MON89788 event.
文摘Perit on eal metastasis is the most comm on patter n of recurre nee and the most freque nt cause of death after surgery in patie nts with gastric can cer.Perit on eal free can cer cells dissem in ated from the primary lesi on site have bee n con sidered the main cause of perit on eal metastasis.Perit on eal lavage cytological exam in ati on(PLC)has bee n show n to be an in depe ndent predictor of gastric cancer relapse after curative resection and poor overall survival.However,the conventional cytological examinations have high rates of false-positive and false-negative findings.To improve the sensitivity,molecular-based methods using reverse transcriptase polymerase chain reaction have been developed for detecting cancer cells in peritoneal wash fluids of patients with gastric cancer.We performed a PubMed search for articles describing PLC in gastric can cer.Releva nt articles were reviewed and data on available outcomes elaborated.The cli nical roles and attributes of PLC in gastric can cer were reviewed,and its future applicati on to this disease is discussed.
文摘Hot components operate in a high-temperature and high-pressure environment. The occurrence of a fault in hot components leads to high economic losses. In general, exhaust gas temperature(EGT) is used to monitor the performance of hot components.However, during the early stages of a failure, the fault information is weak, and is simultaneously affected by various types of interference, such as the complex working conditions, ambient conditions, gradual performance degradation of the compressors and turbines, and noise. Additionally, inadequate effective information of the gas turbine also restricts the establishment of the detection model. To solve the above problems, this paper proposes an anomaly detection method based on frequent pattern extraction. A frequent pattern model(FPM) is applied to indicate the inherent regularity of change in EGT occurring from different types of interference. In this study, based on a genetic algorithm and support vector machine regression, the relationship model between the EGT and interference was tentatively built. The modeling accuracy was then further improved through the selection of the kernel function and training data. Experiments indicate that the optimal kernel function is linear and that the optimal training data should be balanced in addition to covering the appropriate range of operating conditions and ambient temperature. Furthermore, the thresholds based on the Pauta criterion that is automatically obtained during the modeling process, are used to determine whether hot components are operating abnormally. Moreover, the FPM is compared with the similarity theory, which demonstrates that the FPM can better suppress the effect of the component performance degradation and fuel heat value fluctuation. Finally, the effectiveness of the proposed method is validated on seven months of actual data obtained from a Titan130 gas turbine on an offshore oil platform. The results indicate that the proposed method can sensitively detect malfunctions in hot components during the early stages of a fault, and is robust to various types of interference.
基金funded by the Deep Earth Probe and Mineral Resources Exploration-National Science and Technology Major Project(No.2025ZD1010904)Interdisciplinary Research Project for Young Teachers of USTB(Fundamental Research Funds for the Central Universities,No.FRF-IDRY-24-016)+3 种基金Beijing Natural Science Foundation(No.2232080)the National Natural Science Foundation of China(Nos.52374112,52204124,52034001,and 52304144)the State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering(No.SDGZ2517)the Shandong Provincial Natural Science Foundation(No.ZR2023QE133)
文摘Fluidized bioleaching is an efficient,environmentally friendly,and cost-effective mining method that has been widely explored and utilized for recovering low-grade copper sulfide minerals,such as chalcopyrite.However,the proliferation and apoptosis of dominant leaching bacteria,such as Acidithiobacillus ferrooxidans,within complex pore,void,and fracture structures in deep-earth environments commonly results in a dynamic bacterial community that evolves continuously.This unclear genetic-scale microbial succession often leads to low leaching reaction efficiency,undesirable reaction passivation,and poor bioleaching operations.This review integrates genetic-scale insights with industrial challenges in chalcopyrite bioleaching,proposing novel strategies for regulating microbial communities.A systematic analysis of five critical dimensions is conducted,focusing on:1)The adaptations of Acidithiobacillus spp.to high Ag^(+) stress.2)The direct,indirect,and cooperative bioleaching pathways are linked to bacterial extracellular polymer substance(EPS)and Fe/S oxidation genes.3)The passivation dynamics governed by bacterial genomics,including thiosulfate,polysulfide,and biofilm mechanisms.4)The microbial succession patterns under genetic control Hi-C sequencing-guided consortia design.5)Molecular detection methods(16S rDNA,Hi-C)for optimizing leaching efficiency.The following innovations have been identified as being of key significance:A genomic-environmental interaction model has been developed to bridge the gap between bacterial genetics and passivation dynamics.A comprehensive analysis of Ag^(+) catalysis has been conducted,resulting in a 40%reduction in jarosite formation through jar gene suppression.Practical strategies,such as thermophilic consortia engineering,have been validated in pilot trials,achieving a 32%increase in copper recovery.Additionally,this study meticulously reviews and summarizes typical potential stimulations and enhanced bioleaching methods.The genetic sequencing methods,such as 16S rDNA and Hi-C,have been shown to hold promising potential for improving bioleaching reactions and delaying the formation of passivation substances like jarosite.
文摘Objective To conduct clinical analysis and genetic variant detection in patients with congenital hypothyroidism,and to explore the role of paired box 8 (PAX8) gene variants in the pathogenic mechanism of congenital hypothyroidism,thereby providing insights for its diagnosis and management.
