Tal fescue （Festuca arundinacea Schreb.） is an important cool-season forage grasses and turfgrasses at home and abroad. In order to provide a reference for tal fescue breeding, we reviewed the genetic constitution a...Tal fescue （Festuca arundinacea Schreb.） is an important cool-season forage grasses and turfgrasses at home and abroad. In order to provide a reference for tal fescue breeding, we reviewed the genetic constitution and heritability, endo-phytic fungi and new tal fescue variety breeding, application of modern biological technology （expressed sequence tag, genetic linkage map construction and trans-genic breeding） in breeding new varieties of tal fescue and the breeding status of tal fescue in Guizhou region.展开更多
Sweet sorghum is a crop with good application prospects, and the research on sweet sorghum breeding people should be strengthened. Based on this, the genetic content of QTLs(quantitative trait loci) for sugar traits i...Sweet sorghum is a crop with good application prospects, and the research on sweet sorghum breeding people should be strengthened. Based on this, the genetic content of QTLs(quantitative trait loci) for sugar traits in sweet sorghum was introduced, and the analysis content of the genetic breeding of sweet sorghum related to sugar traits was expounded, providing support for the cultivation of sweet sorghum with higher quality and the promotion of agricultural development in China.展开更多
Nicotiana tabacum(2n=4x=48),an economically important non-food crop and a model plant for genetic studies,faces challenges in efficient genotyping of novel germplasm.To address this,we developed the Ta-LD-SC,a 20K SNP...Nicotiana tabacum(2n=4x=48),an economically important non-food crop and a model plant for genetic studies,faces challenges in efficient genotyping of novel germplasm.To address this,we developed the Ta-LD-SC,a 20K SNP Affymetrix Axiom array,based on resequencing data from 150 tobacco accessions.A total of 20,213 unique SNPs were carefully selected,achieving coverage of over 90%of the tobacco genome(Nitab4.5 and NtaSR1)with a uniform probe distribution,limiting density to no more than 5 SNPs per 200 kb.The array underwent extensive validation using 866 tobacco accessions(NP panel)and 288 F2 individuals from a cross between K326 and Oxford 26(GP panel).Performance metrics demonstrated its robustness,with high SNP call rates(93.6%-99.8%),a low technical error rate(<1%),and a superior PolyHighResolution SNP rate(79.79%)compared to other crop SNP arrays.Population structure analysis of the NP panel revealed two major introductions of foreign germplasm that have significantly influenced the genetic diversity of Chinese tobacco resources.Using the array,a genome-wide association study(GWAS)identified 62 genes linked to eight agronomic traits,and a high-density genetic map encompassing 4553 SNPs across 6606.08 cM was constructed.The Ta-LD-SC array provides a valuable tool for rapid,high-quality genotyping offering supporting marker annotations that may benefit genetic research and breeding of tobacco.展开更多
Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties.For the purpose of genetic improvement,researchers can select for desirable genetic traits,integrate a suite of...Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties.For the purpose of genetic improvement,researchers can select for desirable genetic traits,integrate a suite of traits from different donors,or alter the innate genetic traits of a species.These improved varieties have,in many cases,facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield.In this review,we present the pertinent literatures and summarize the biological bases and application of selection breeding technologies(containing traditional selective breeding,molecular marker-assisted breeding,genome-wide selective breeding and breeding by controlling single-sex groups),integration breeding technologies(containing cross breeding,nuclear transplantation,germline stem cells and germ cells transplantation,artificial gynogenesis,artificial androgenesis and polyploid breeding)and modification breeding technologies(represented by transgenic breeding)in fish genetic breeding.Additionally,we discuss the progress our laboratory has made in the field of chromosomal ploidy breeding of fish,including distant hybridization,gynogenesis,and androgenesis.Finally,we systematically summarize the research status and known problems associated with each technology.展开更多
Genomics focuses on dissection of genome structure and function to provide a molecular basis for understanding the genetic background. In a pivotal step,the expense of whole genome sequencing has been largely eliminat...Genomics focuses on dissection of genome structure and function to provide a molecular basis for understanding the genetic background. In a pivotal step,the expense of whole genome sequencing has been largely eliminated by the rapid updating of sequencing technology,leading to increasing numbers of decoded genomes of aquatic organisms, driving the aquaculture industry into the genomic era. Multiple aquatic areas have been influenced by these findings, such as accelerated generation shift in the seed industry and the process of breeding improved lines. In this article, we have summarized the latest domestic and international progress of aquatic animals in nine aspects, including WGS and fine mapping,construction of high density genetic/physical maps, traitrelated marker/genes screening, as well as sex control,genome editing, and other molecular breeding technologies. Finally, the existing problems in this field have been discussed and five future counter measures have been proposed accordingly.展开更多
Wheat germplasm is a fundamental resource for basic research,applied studies,and wheat breeding,which can be enriched normally by several paths,such as collecting natural lines,accumulating breeding lines,and introduc...Wheat germplasm is a fundamental resource for basic research,applied studies,and wheat breeding,which can be enriched normally by several paths,such as collecting natural lines,accumulating breeding lines,and introducing mutagenesis materials.Ethyl methane sulfonate(EMS)is an alkylating agent that can effectively introduce genetic variations in a wide variety of plant species.In this study,we created a million-scale EMS population(MEP)that started with the Chinese wheat cultivars‘Luyan 128’,‘Jimai 38’,‘Jimai 44’,and‘Shannong 30’.In the M1 generation,the MEP had numerous phenotypical variations,such as>3,000 chlorophyll-deficient mutants,2,519 compact spikes,and 1,692 male sterile spikes.There were also rare mutations,including 30 independent tillers each with double heads.Some M1 variations of chlorophyll-deficiency and compact spikes were inheritable,appearing in the M2 or M3 generations.To advance the entire MEP to higher generations,we adopted a single-seed descendent(SSD)approach.All other seed composites of M2 were used to screen other agronomically important traits,such as the tolerance to herbicide quizalofop-P-methyl.The MEP is available for collaborative projects,and provides a valuable toolbox for wheat genetics and breeding for sustainable agriculture.展开更多
Tree Genetics and Molecular Breeding(ISSN 1927-5781)is an international,open access,peer reviewed journal,committed to serve for tree genetics and molecular breeding,particularly publishing innovative research finding...Tree Genetics and Molecular Breeding(ISSN 1927-5781)is an international,open access,peer reviewed journal,committed to serve for tree genetics and molecular breeding,particularly publishing innovative research findings in the basic and applied fields of tree molecular genetics and novel techniques for fruit/forest/ornamental/展开更多
CRISPR-Cas9 has emerged as a powerful tool for gene editing,and it has been widely used in plant functional genomics research and crop genetic breeding(Chen et al.2019).The target specificity of CRISPR-Cas9 relies on ...CRISPR-Cas9 has emerged as a powerful tool for gene editing,and it has been widely used in plant functional genomics research and crop genetic breeding(Chen et al.2019).The target specificity of CRISPR-Cas9 relies on the 20-base-pair single guide RNA(sgRNA),which makes creating plant-specific mutant libraries through large-scale synthesis of sgRNAs targeting multiple genes or even the whole genome relatively quick and straightforward.展开更多
[Objectives]This study was conducted to improve the production efficiency of dark-shank partridge-like plumage high-quality broilers and ensure animal welfare,so as to meet the development needs of China’s yellow-fea...[Objectives]This study was conducted to improve the production efficiency of dark-shank partridge-like plumage high-quality broilers and ensure animal welfare,so as to meet the development needs of China’s yellow-feather broiler industry.[Methods]Sichuan Animal Science Academy and Sichuan Dahen Poultry Breeding Co.,Ltd.formed a joint breeding research team.The team bred Dahen 799 broiler complete set line with local chicken breeds as the main material and improving production efficiency,stabilizing meat quality and flavor and achieving autosexing of chicks as the main breeding goals by modern poultry breeding technologies,after 10 years and 8 generations.[Results]The Dahen 799 broiler complete set line was approved by National Commission of Livestock and Poultry Genetic Resources in December 2020.The 66-week-old egg production of the breed reached 189 eggs.For the commercial generation,cocks and hens showed 10-week-old weights of 2668 and 2269 g,respectively;the feed conversion ratio was 2.26-2.35;the survivability was 96.7%-97.2%;and the average intramuscular fat contents of cocks and hens were in the range of 1.8%-2.1%,and the inosinic acid contents were in the range of 2.3-3.2 mg/g.The production performance and meat flavor of Dahen 799 broilers were better than Dahen 699 broilers,Sichuan mountainous bone-black chickens and two other social breeds.Compared with Sichuan mountainous bone-black chickens,the egg production at the age of 66 weeks increased by 47 eggs;the average weight of cocks and hens of the commercial generation at 10 weeks increased by 45.21%;and the autosexing rate of chicks of the commercial generation reached 99.3%.[Conclusions]Dahen 799 broilers have the advantages of good production performance,high feed conversion ratio and excellent meat flavor and the characteristic of autosexing which other breeds do not have,and can fully meet the industrial development needs of dark shanks and partridge-like plumage broilers.展开更多
Over the last 100 years,significant advances have been made in the characterisation of milk composition for dairy cattle improvement programs.Technological progress has enabled a shift from labour intensive,on-farm co...Over the last 100 years,significant advances have been made in the characterisation of milk composition for dairy cattle improvement programs.Technological progress has enabled a shift from labour intensive,on-farm collection and processing of samples that assess yield and fat levels in milk,to large-scale processing of samples through centralised laboratories,with the scope extended to include quantification of other traits.Fourier-transform midinfrared(FT-MIR)spectroscopy has had a significant role in the transformation of milk composition phenotyping,with spectral-based predictions of major milk components already being widely used in milk payment and animal evaluation systems globally.Increasingly,there is interest in analysing the individual FT-MIR wavenumbers,and in utilising the FT-MIR data to predict other novel traits of importance to breeding programs.This includes traits related to the nutritional value of milk,the processability of milk into products such as cheese,and traits relevant to animal health and the environment.The ability to successfully incorporate these traits into breeding programs is dependent on the heritability of the FT-MIR predicted traits,and the genetic correlations between the FT-MIR predicted and actual trait values.Linking FT-MIR predicted traits to the underlying mutations responsible for their variation can be difficult because the phenotypic expression of these traits are a function of a diverse range of molecular and biological mechanisms that can obscure their genetic basis.The individual FT-MIR wavenumbers give insights into the chemical composition of milk and provide an additional layer of granularity that may assist with establishing causal links between the genome and observed phenotypes.Additionally,there are other molecular phenotypes such as those related to the metabolome,chromatin accessibility,and RNA editing that could improve our understanding of the underlying biological systems controlling traits of interest.Here we review topics of importance to phenotyping and genetic applications of FT-MIR spectra datasets,and discuss opportunities for consolidating FT-MIR datasets with other genomic and molecular data sources to improve future dairy cattle breeding programs.展开更多
Receptor-like kinases(RLKs)and receptor-like cytoplasmic kinases(RLCKs)play an indispensable role in the perception and transmission of extracellular signals in plants.In rice,these kinases actively participate in imm...Receptor-like kinases(RLKs)and receptor-like cytoplasmic kinases(RLCKs)play an indispensable role in the perception and transmission of extracellular signals in plants.In rice,these kinases actively participate in immune responses against a variety of pathogens,including fungi,bacteria,and viruses.However,research on the specific response mechanisms and the spectrum of different kinase activities against various pathogens remains insufficient.This review provides an in-depth and comprehensive overview of the types and functions of RLKs and RLCKs involved in disease resistance,emphasizing the central role of certain RLKs and RLCKs in the plant immune system.These kinases can recognize specific molecular patterns of pathogens and rapidly initiate an immune response in rice.Furthermore,the activity and functional regulation of these key kinases are tightly controlled by various post-translational modifications,such as phosphorylation and ubiquitination.This meticulous regulation ensures that the rice immune system's response is both precise and timely,effectively balancing the intensity of the immune response and preventing potential issues caused by either hyperactivity or insufficiency.By synthesizing current research findings,this review not only broadens our understanding of the role of RLKs and RLCKs in plant immunity but also provides new perspectives and strategies for future research on disease resistance breeding in rice.Future studies are expected to delve deeper into the signaling networks and regulatory mechanisms of these kinases,exploring their potential in agricultural production to develop rice varieties with enhanced disease resistance.展开更多
In vitro production of functional gametes can revolutionize reproduction by reducing generation intervals and accelerating genetic breeding in aquaculture,especially in fish with relatively long generations.Neverthele...In vitro production of functional gametes can revolutionize reproduction by reducing generation intervals and accelerating genetic breeding in aquaculture,especially in fish with relatively long generations.Nevertheless,functional sperm production from in vitro-cultured spermatogonia remains a challenge in most aquaculture fish.In this study,we isolated and characterized premeiotic spermatogonia from marine four-eyed sleepers(Bostrychus sinensis),which are prone to ovotesticular or sterile testicular development,and induced the differentiation of the spermatogonia into flagellated sperm in a three-dimensional(3D)culture system.Artificial insemination indicated that the in vitro-derived sperm were capable of fertilizing mature oocytes to develop into normal larvae.Furthermore,melatonin significantly promoted spermatogonia proliferation and differentiation through the ERK1/2 signaling pathway,and thus increased the efficiency in functional sperm production.The 3D culture system and resulting functional sperm hold great promise for improving the genetic breeding of aquaculture fish.展开更多
Single-cell RNA sequencing technology(scRNA-seq)is an emerging,high-throughput,high-resolution big data production and analysis technology,which has helped some pioneering research and discoveries in the field of anim...Single-cell RNA sequencing technology(scRNA-seq)is an emerging,high-throughput,high-resolution big data production and analysis technology,which has helped some pioneering research and discoveries in the field of animal husbandry in recent years.This technology provides an essential basis for the early selection of livestock and poultry and the selection of excellent characters,showing that it has great application potential and broad prospects in animal husbandry research and production.Although scRNA-seq technology has established a wide range of commercial production services at home and abroad,the standardization status of this technology in animal husbandry needs further exploration,as it can provide clues and foundations for the application of scRNA-seq technology in animal husbandry.展开更多
The objective of this study was to develop a method to assess and analyze the total allelopathic potential of crop germplasm and to test this method on four winter wheat accessions commonly planted in the Loess Platea...The objective of this study was to develop a method to assess and analyze the total allelopathic potential of crop germplasm and to test this method on four winter wheat accessions commonly planted in the Loess Plateau. A systems engineering model was developed and used to evaluate the total allelopathic potential of crop cultivars. In addition, a method for quantifying the total allelopathic potential in crop accessions was presented. Total allelopathic potential of four winter wheat accessions from the Loess Plateau was estimated and compared using a systems theory approach. The model assessed allelopathic potential in different parts of the plants from the time wheat turned green in spring until maturity. Results from these models indicated that the four wheat accessions had very weak allelopathic potential. Allelopathic potential declined in the order Xiaoyan 22 〉 Ningdong 1 〉 Fengchan 3 〉 Bima 1. This system engineering evaluation method allows for the assessment of allelopathic potential among crop varieties. It will help plant breeders to select and develop allelopathic crop accessions that combine weed suppression properties with agronomic traits related to yield and quality.展开更多
Sorghum is an important source of food, feed and raw material for brewing, and is expected to be a promising bioenergy crop. Sorghum is well known for its strong resistance to abiotic stress and wide adaptability, and...Sorghum is an important source of food, feed and raw material for brewing, and is expected to be a promising bioenergy crop. Sorghum is well known for its strong resistance to abiotic stress and wide adaptability, and salt tolerance is one of its main characteristics. Increasing sorghum planting acreage on saline-alkalien land is one way to effectively use this kind of marginal soil. In this paper, domestic and overseas research on plant tolerance to soil salinity and alkalinity in sorghum, including salt-tolerant genetics and breeding, physiology, cultivation, and identification of tolerant germplasms, are reviewed. Suggestions for further studies on salinity and alkalinity tolerance in sorghum are given, and the prospects for sorghum production in saline-alkalien land are discussed.展开更多
Phosphorus is the second most important macronutrient after nitrogen and it has many vital functions in the life of plants.Most soils have a low available P content,which has become a key limiting factor for increasin...Phosphorus is the second most important macronutrient after nitrogen and it has many vital functions in the life of plants.Most soils have a low available P content,which has become a key limiting factor for increasing crop production.Also,low P use efficiency(PUE)of crops in conjunction with excessive application of P fertilizers has resulted in serious environmental problems.Thus,dissecting the genetic architecture of crop PUE,mining related quantitative trait loci(QTL)and using molecular breeding methods to improve high PUE germplasm are of great significance and serve as an efficient approach for the development of sustainable agriculture.In this review,molecular and phenotypic characteristics of maize inbred lines with high PUE,related QTL and genes as well as low-P responses are summarized.Based on this,a breeding strategy applying genomic selection as the core,and integrating the existing genetic information and molecular breeding techniques is proposed for breeding high PUE maize inbred lines and hybrids.展开更多
The research focuses on the study of anatomical and morphological stalk structure ofsoya interspecific hybrids of the third generation （F3） between （Glycine max （L.） Merr.） and G. soja Sieb. et Zucc. in comparis...The research focuses on the study of anatomical and morphological stalk structure ofsoya interspecific hybrids of the third generation （F3） between （Glycine max （L.） Merr.） and G. soja Sieb. et Zucc. in comparison with parent plant species. The parent plant species and interspecific hybrids were sowed and grew under similar conditions. The similarity of the anatomic structure of stalks of cultivated plants and Glycine soja （wild soya） proves the hypothesis the studied species have the same origin. However, the obtained results show the considerable degree of phylogenetic dissociation between the studied soya species. Interspecific hybrids inherit from G. soja the ability to high intensive growth. The G. soja use in practical selective breeding is of great interest.展开更多
In the coming decades,plant agriculture must produce the food needed to sustain the world＇s burgeoning population.This challenge is made more daunting in light of a changing climate,scarcity of inputs such as water a...In the coming decades,plant agriculture must produce the food needed to sustain the world＇s burgeoning population.This challenge is made more daunting in light of a changing climate,scarcity of inputs such as water and fertilizer,and the need to grow more crops on increasingly marginal land.To achieve food security,we will need to fully exploit plant genetics to develop new crop varieties that produce higher yields of healthier food.展开更多
With the support by the National Natural Science Foundation of China and the'Strategic Priority Research Program'of the Chinese Academy of Sciences,a collaborative study by the research groups led by Professor...With the support by the National Natural Science Foundation of China and the'Strategic Priority Research Program'of the Chinese Academy of Sciences,a collaborative study by the research groups led by Professors Tian Zhixi(田志喜),Wang Guodong(王国栋),and Zhu Baoge(朱保葛)from the展开更多
Zhang Xianlong, male, born in March 1963, from Zhumadian, Henan Province, is a professor at Huazhong Agricultural University. He graduated from the Department of Agronomy at Huazhong Agricultural University in 1984 an...Zhang Xianlong, male, born in March 1963, from Zhumadian, Henan Province, is a professor at Huazhong Agricultural University. He graduated from the Department of Agronomy at Huazhong Agricultural University in 1984 and obtained his master's and doctoral degrees in crop genetics and breeding from the same university in 1987 and 1990, respectively.展开更多
基金Supported by the Key Agricultural Technology R&D Program of Guizhou Province(Qiankehe NY(2008)3070Qiankehe NY(2010)3044)+1 种基金the Foundation for Science andTechnology of Guizhou Province(Qiankehe J(2009)2133Qiankehe J(2012)2202)~~
文摘Tal fescue （Festuca arundinacea Schreb.） is an important cool-season forage grasses and turfgrasses at home and abroad. In order to provide a reference for tal fescue breeding, we reviewed the genetic constitution and heritability, endo-phytic fungi and new tal fescue variety breeding, application of modern biological technology （expressed sequence tag, genetic linkage map construction and trans-genic breeding） in breeding new varieties of tal fescue and the breeding status of tal fescue in Guizhou region.
文摘Sweet sorghum is a crop with good application prospects, and the research on sweet sorghum breeding people should be strengthened. Based on this, the genetic content of QTLs(quantitative trait loci) for sugar traits in sweet sorghum was introduced, and the analysis content of the genetic breeding of sweet sorghum related to sugar traits was expounded, providing support for the cultivation of sweet sorghum with higher quality and the promotion of agricultural development in China.
基金supported by the Guizhou Provincial Basic Research Program(Natural Science)[(2024)648]the Program of China National Tobacco Corporation(110202101032(JY-09),110202201003(JY-03))the Program of Guizhou Branch of China National Tobacco Corporation(2023XM02,2021XM05,2022XM05,2024XM01).
文摘Nicotiana tabacum(2n=4x=48),an economically important non-food crop and a model plant for genetic studies,faces challenges in efficient genotyping of novel germplasm.To address this,we developed the Ta-LD-SC,a 20K SNP Affymetrix Axiom array,based on resequencing data from 150 tobacco accessions.A total of 20,213 unique SNPs were carefully selected,achieving coverage of over 90%of the tobacco genome(Nitab4.5 and NtaSR1)with a uniform probe distribution,limiting density to no more than 5 SNPs per 200 kb.The array underwent extensive validation using 866 tobacco accessions(NP panel)and 288 F2 individuals from a cross between K326 and Oxford 26(GP panel).Performance metrics demonstrated its robustness,with high SNP call rates(93.6%-99.8%),a low technical error rate(<1%),and a superior PolyHighResolution SNP rate(79.79%)compared to other crop SNP arrays.Population structure analysis of the NP panel revealed two major introductions of foreign germplasm that have significantly influenced the genetic diversity of Chinese tobacco resources.Using the array,a genome-wide association study(GWAS)identified 62 genes linked to eight agronomic traits,and a high-density genetic map encompassing 4553 SNPs across 6606.08 cM was constructed.The Ta-LD-SC array provides a valuable tool for rapid,high-quality genotyping offering supporting marker annotations that may benefit genetic research and breeding of tobacco.
基金supported by the Major International Cooperation Projects of the National Natural Science Foundation of China(31210103918)National Natural Science Foundation of China(31430088 and 31272651)+3 种基金National High Technology Research and Development Program of China(2011AA100403)Doctoral Fund of Ministry of Education of China(20114306130001)Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province(20134486)the Construct Program of the Key Discipline in Hunan Province and China
文摘Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties.For the purpose of genetic improvement,researchers can select for desirable genetic traits,integrate a suite of traits from different donors,or alter the innate genetic traits of a species.These improved varieties have,in many cases,facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield.In this review,we present the pertinent literatures and summarize the biological bases and application of selection breeding technologies(containing traditional selective breeding,molecular marker-assisted breeding,genome-wide selective breeding and breeding by controlling single-sex groups),integration breeding technologies(containing cross breeding,nuclear transplantation,germline stem cells and germ cells transplantation,artificial gynogenesis,artificial androgenesis and polyploid breeding)and modification breeding technologies(represented by transgenic breeding)in fish genetic breeding.Additionally,we discuss the progress our laboratory has made in the field of chromosomal ploidy breeding of fish,including distant hybridization,gynogenesis,and androgenesis.Finally,we systematically summarize the research status and known problems associated with each technology.
基金supported by grants from the National Natural Science Foundation of China (31461163005, 31530078)the Taishan Scholar Climbing Project Fund of Shandong of China
文摘Genomics focuses on dissection of genome structure and function to provide a molecular basis for understanding the genetic background. In a pivotal step,the expense of whole genome sequencing has been largely eliminated by the rapid updating of sequencing technology,leading to increasing numbers of decoded genomes of aquatic organisms, driving the aquaculture industry into the genomic era. Multiple aquatic areas have been influenced by these findings, such as accelerated generation shift in the seed industry and the process of breeding improved lines. In this article, we have summarized the latest domestic and international progress of aquatic animals in nine aspects, including WGS and fine mapping,construction of high density genetic/physical maps, traitrelated marker/genes screening, as well as sex control,genome editing, and other molecular breeding technologies. Finally, the existing problems in this field have been discussed and five future counter measures have been proposed accordingly.
基金This work was supported by the National Key Research and Development Program of China(2022YFF1002300)the Quancheng‘5150’Talent Program,China(07962021047)the Agriculture Applied Technology Initiative of Jinan Government,China(CX202113).
文摘Wheat germplasm is a fundamental resource for basic research,applied studies,and wheat breeding,which can be enriched normally by several paths,such as collecting natural lines,accumulating breeding lines,and introducing mutagenesis materials.Ethyl methane sulfonate(EMS)is an alkylating agent that can effectively introduce genetic variations in a wide variety of plant species.In this study,we created a million-scale EMS population(MEP)that started with the Chinese wheat cultivars‘Luyan 128’,‘Jimai 38’,‘Jimai 44’,and‘Shannong 30’.In the M1 generation,the MEP had numerous phenotypical variations,such as>3,000 chlorophyll-deficient mutants,2,519 compact spikes,and 1,692 male sterile spikes.There were also rare mutations,including 30 independent tillers each with double heads.Some M1 variations of chlorophyll-deficiency and compact spikes were inheritable,appearing in the M2 or M3 generations.To advance the entire MEP to higher generations,we adopted a single-seed descendent(SSD)approach.All other seed composites of M2 were used to screen other agronomically important traits,such as the tolerance to herbicide quizalofop-P-methyl.The MEP is available for collaborative projects,and provides a valuable toolbox for wheat genetics and breeding for sustainable agriculture.
文摘Tree Genetics and Molecular Breeding(ISSN 1927-5781)is an international,open access,peer reviewed journal,committed to serve for tree genetics and molecular breeding,particularly publishing innovative research findings in the basic and applied fields of tree molecular genetics and novel techniques for fruit/forest/ornamental/
基金supported by the National Natural Science Foundation of China(32272670 and 31972986)the Key Research and Development Program of Shaanxi Province,China(2023-YBNY-059)。
文摘CRISPR-Cas9 has emerged as a powerful tool for gene editing,and it has been widely used in plant functional genomics research and crop genetic breeding(Chen et al.2019).The target specificity of CRISPR-Cas9 relies on the 20-base-pair single guide RNA(sgRNA),which makes creating plant-specific mutant libraries through large-scale synthesis of sgRNAs targeting multiple genes or even the whole genome relatively quick and straightforward.
基金Supported by National Broiler Industry Technology System(CARS-41-04)Sichuan Province Key R&D Program(2021YFYZ0031,2021YFN0029)Sichuan Provincial Financial Operation Fund(SASA2020CZYX002,SASA2020CZYX007)。
文摘[Objectives]This study was conducted to improve the production efficiency of dark-shank partridge-like plumage high-quality broilers and ensure animal welfare,so as to meet the development needs of China’s yellow-feather broiler industry.[Methods]Sichuan Animal Science Academy and Sichuan Dahen Poultry Breeding Co.,Ltd.formed a joint breeding research team.The team bred Dahen 799 broiler complete set line with local chicken breeds as the main material and improving production efficiency,stabilizing meat quality and flavor and achieving autosexing of chicks as the main breeding goals by modern poultry breeding technologies,after 10 years and 8 generations.[Results]The Dahen 799 broiler complete set line was approved by National Commission of Livestock and Poultry Genetic Resources in December 2020.The 66-week-old egg production of the breed reached 189 eggs.For the commercial generation,cocks and hens showed 10-week-old weights of 2668 and 2269 g,respectively;the feed conversion ratio was 2.26-2.35;the survivability was 96.7%-97.2%;and the average intramuscular fat contents of cocks and hens were in the range of 1.8%-2.1%,and the inosinic acid contents were in the range of 2.3-3.2 mg/g.The production performance and meat flavor of Dahen 799 broilers were better than Dahen 699 broilers,Sichuan mountainous bone-black chickens and two other social breeds.Compared with Sichuan mountainous bone-black chickens,the egg production at the age of 66 weeks increased by 47 eggs;the average weight of cocks and hens of the commercial generation at 10 weeks increased by 45.21%;and the autosexing rate of chicks of the commercial generation reached 99.3%.[Conclusions]Dahen 799 broilers have the advantages of good production performance,high feed conversion ratio and excellent meat flavor and the characteristic of autosexing which other breeds do not have,and can fully meet the industrial development needs of dark shanks and partridge-like plumage broilers.
基金funded by Livestock Improvement Corporation(LIC)the New Zealand Ministry for Primary Industries,through the Sustainable Food&Fibre Futures programme.
文摘Over the last 100 years,significant advances have been made in the characterisation of milk composition for dairy cattle improvement programs.Technological progress has enabled a shift from labour intensive,on-farm collection and processing of samples that assess yield and fat levels in milk,to large-scale processing of samples through centralised laboratories,with the scope extended to include quantification of other traits.Fourier-transform midinfrared(FT-MIR)spectroscopy has had a significant role in the transformation of milk composition phenotyping,with spectral-based predictions of major milk components already being widely used in milk payment and animal evaluation systems globally.Increasingly,there is interest in analysing the individual FT-MIR wavenumbers,and in utilising the FT-MIR data to predict other novel traits of importance to breeding programs.This includes traits related to the nutritional value of milk,the processability of milk into products such as cheese,and traits relevant to animal health and the environment.The ability to successfully incorporate these traits into breeding programs is dependent on the heritability of the FT-MIR predicted traits,and the genetic correlations between the FT-MIR predicted and actual trait values.Linking FT-MIR predicted traits to the underlying mutations responsible for their variation can be difficult because the phenotypic expression of these traits are a function of a diverse range of molecular and biological mechanisms that can obscure their genetic basis.The individual FT-MIR wavenumbers give insights into the chemical composition of milk and provide an additional layer of granularity that may assist with establishing causal links between the genome and observed phenotypes.Additionally,there are other molecular phenotypes such as those related to the metabolome,chromatin accessibility,and RNA editing that could improve our understanding of the underlying biological systems controlling traits of interest.Here we review topics of importance to phenotyping and genetic applications of FT-MIR spectra datasets,and discuss opportunities for consolidating FT-MIR datasets with other genomic and molecular data sources to improve future dairy cattle breeding programs.
基金supported by the National Natural Science Foundation of China (Grant No.U2005211)the Fuzhou General Teaching Hospital (the 900th Hospital)Key Project,China (Grant No.2022ZD01)the Fujian Clinical Research Center for Aptamer-based Precision Testing,China (Grant No.2021Y2017)。
文摘Receptor-like kinases(RLKs)and receptor-like cytoplasmic kinases(RLCKs)play an indispensable role in the perception and transmission of extracellular signals in plants.In rice,these kinases actively participate in immune responses against a variety of pathogens,including fungi,bacteria,and viruses.However,research on the specific response mechanisms and the spectrum of different kinase activities against various pathogens remains insufficient.This review provides an in-depth and comprehensive overview of the types and functions of RLKs and RLCKs involved in disease resistance,emphasizing the central role of certain RLKs and RLCKs in the plant immune system.These kinases can recognize specific molecular patterns of pathogens and rapidly initiate an immune response in rice.Furthermore,the activity and functional regulation of these key kinases are tightly controlled by various post-translational modifications,such as phosphorylation and ubiquitination.This meticulous regulation ensures that the rice immune system's response is both precise and timely,effectively balancing the intensity of the immune response and preventing potential issues caused by either hyperactivity or insufficiency.By synthesizing current research findings,this review not only broadens our understanding of the role of RLKs and RLCKs in plant immunity but also provides new perspectives and strategies for future research on disease resistance breeding in rice.Future studies are expected to delve deeper into the signaling networks and regulatory mechanisms of these kinases,exploring their potential in agricultural production to develop rice varieties with enhanced disease resistance.
基金supported by the National Key R&D Program of China(2018YFD0901205)National Natural Science Foundation of China(31771587,31970535)Guangdong Basic and Applied Basic Research Foundation(2020A1515010358)。
文摘In vitro production of functional gametes can revolutionize reproduction by reducing generation intervals and accelerating genetic breeding in aquaculture,especially in fish with relatively long generations.Nevertheless,functional sperm production from in vitro-cultured spermatogonia remains a challenge in most aquaculture fish.In this study,we isolated and characterized premeiotic spermatogonia from marine four-eyed sleepers(Bostrychus sinensis),which are prone to ovotesticular or sterile testicular development,and induced the differentiation of the spermatogonia into flagellated sperm in a three-dimensional(3D)culture system.Artificial insemination indicated that the in vitro-derived sperm were capable of fertilizing mature oocytes to develop into normal larvae.Furthermore,melatonin significantly promoted spermatogonia proliferation and differentiation through the ERK1/2 signaling pathway,and thus increased the efficiency in functional sperm production.The 3D culture system and resulting functional sperm hold great promise for improving the genetic breeding of aquaculture fish.
文摘Single-cell RNA sequencing technology(scRNA-seq)is an emerging,high-throughput,high-resolution big data production and analysis technology,which has helped some pioneering research and discoveries in the field of animal husbandry in recent years.This technology provides an essential basis for the early selection of livestock and poultry and the selection of excellent characters,showing that it has great application potential and broad prospects in animal husbandry research and production.Although scRNA-seq technology has established a wide range of commercial production services at home and abroad,the standardization status of this technology in animal husbandry needs further exploration,as it can provide clues and foundations for the application of scRNA-seq technology in animal husbandry.
文摘The objective of this study was to develop a method to assess and analyze the total allelopathic potential of crop germplasm and to test this method on four winter wheat accessions commonly planted in the Loess Plateau. A systems engineering model was developed and used to evaluate the total allelopathic potential of crop cultivars. In addition, a method for quantifying the total allelopathic potential in crop accessions was presented. Total allelopathic potential of four winter wheat accessions from the Loess Plateau was estimated and compared using a systems theory approach. The model assessed allelopathic potential in different parts of the plants from the time wheat turned green in spring until maturity. Results from these models indicated that the four wheat accessions had very weak allelopathic potential. Allelopathic potential declined in the order Xiaoyan 22 〉 Ningdong 1 〉 Fengchan 3 〉 Bima 1. This system engineering evaluation method allows for the assessment of allelopathic potential among crop varieties. It will help plant breeders to select and develop allelopathic crop accessions that combine weed suppression properties with agronomic traits related to yield and quality.
基金supported by the earmarked fund for China Agriculture Research System(CARS-06)
文摘Sorghum is an important source of food, feed and raw material for brewing, and is expected to be a promising bioenergy crop. Sorghum is well known for its strong resistance to abiotic stress and wide adaptability, and salt tolerance is one of its main characteristics. Increasing sorghum planting acreage on saline-alkalien land is one way to effectively use this kind of marginal soil. In this paper, domestic and overseas research on plant tolerance to soil salinity and alkalinity in sorghum, including salt-tolerant genetics and breeding, physiology, cultivation, and identification of tolerant germplasms, are reviewed. Suggestions for further studies on salinity and alkalinity tolerance in sorghum are given, and the prospects for sorghum production in saline-alkalien land are discussed.
基金supported by the National Key Research and Development Program of China (2018YFD0100201 and 2016YFD0101201)the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of Chinathe Sino-German International Research Training Group “Adaptation of maize-based food-feed-energy systems to limited phosphate resources.”
文摘Phosphorus is the second most important macronutrient after nitrogen and it has many vital functions in the life of plants.Most soils have a low available P content,which has become a key limiting factor for increasing crop production.Also,low P use efficiency(PUE)of crops in conjunction with excessive application of P fertilizers has resulted in serious environmental problems.Thus,dissecting the genetic architecture of crop PUE,mining related quantitative trait loci(QTL)and using molecular breeding methods to improve high PUE germplasm are of great significance and serve as an efficient approach for the development of sustainable agriculture.In this review,molecular and phenotypic characteristics of maize inbred lines with high PUE,related QTL and genes as well as low-P responses are summarized.Based on this,a breeding strategy applying genomic selection as the core,and integrating the existing genetic information and molecular breeding techniques is proposed for breeding high PUE maize inbred lines and hybrids.
文摘The research focuses on the study of anatomical and morphological stalk structure ofsoya interspecific hybrids of the third generation （F3） between （Glycine max （L.） Merr.） and G. soja Sieb. et Zucc. in comparison with parent plant species. The parent plant species and interspecific hybrids were sowed and grew under similar conditions. The similarity of the anatomic structure of stalks of cultivated plants and Glycine soja （wild soya） proves the hypothesis the studied species have the same origin. However, the obtained results show the considerable degree of phylogenetic dissociation between the studied soya species. Interspecific hybrids inherit from G. soja the ability to high intensive growth. The G. soja use in practical selective breeding is of great interest.
基金supported in part by grants from the National Science Foundation(Nos.IOS-1444511and IOS-1339209)
文摘In the coming decades,plant agriculture must produce the food needed to sustain the world＇s burgeoning population.This challenge is made more daunting in light of a changing climate,scarcity of inputs such as water and fertilizer,and the need to grow more crops on increasingly marginal land.To achieve food security,we will need to fully exploit plant genetics to develop new crop varieties that produce higher yields of healthier food.
文摘With the support by the National Natural Science Foundation of China and the'Strategic Priority Research Program'of the Chinese Academy of Sciences,a collaborative study by the research groups led by Professors Tian Zhixi(田志喜),Wang Guodong(王国栋),and Zhu Baoge(朱保葛)from the
文摘Zhang Xianlong, male, born in March 1963, from Zhumadian, Henan Province, is a professor at Huazhong Agricultural University. He graduated from the Department of Agronomy at Huazhong Agricultural University in 1984 and obtained his master's and doctoral degrees in crop genetics and breeding from the same university in 1987 and 1990, respectively.
