目的:通过观察健脾活血方对胃癌前病变大鼠胃黏膜组织中CD44V6、MLH1及MSH2表达的影响,探讨健脾活血方对其干预的作用机制.方法:除正常组外,其他大鼠采用以N-甲基-N-硝基-N-亚硝基胍(N-methyl-N-nitro N-nitrosoguanidine,MNNG)为主同...目的:通过观察健脾活血方对胃癌前病变大鼠胃黏膜组织中CD44V6、MLH1及MSH2表达的影响,探讨健脾活血方对其干预的作用机制.方法:除正常组外,其他大鼠采用以N-甲基-N-硝基-N-亚硝基胍(N-methyl-N-nitro N-nitrosoguanidine,MNNG)为主同时配合0.3g/L雷尼替丁、400 mL/L乙醇及饥饱失常的多因素造模法建立胃癌前病变动物模型.将造模成功的40只大鼠随机分为模型组(0.9%氯化钠溶液)、胃复春组(0.86 g/kg)、健脾活血方高、中、低剂量组(32、16、8 g/kg),每组8只,每组每天给予等量(10 mL/kg)的不同药物灌胃一次,连续10 wk.实验末处死大鼠,给予相应处理后,快速免疫组织化学检测CD44V6、MLH1及MSH2表达情况.结果:模型组CD44V6表达与正常组相比明显升高(5.12±1.96 vs 0.25±0.46,P<0.01);健脾活血方高、中剂量组CD44V6表达与模型组相比均明显降低(2.25±0.71,3.25±0.31vs 5.12±1.96,P<0.01或P<0.05),低剂量组C D44V6表达与模型组比较差异无统计学意义(P>0.05);健脾活血方高剂量组CD44V6表达与胃复春组相比明显降低(2.25±0.71 vs4.62±1.19,P<0.01),中、低剂量组CD44V6表达与胃复春组比较差异无统计学意义(P>0.05).模型组MLH1、MSH2表达与正常组相比均明显降低(3.75±1.04 vs 8.00±0.926;3.62±1.69 vs 7.25±2.12,P<0.01);健脾活血方高、中、低剂量组MLH1、MSH2表达与模型组相比均明显升高(6.50±0.93,5.25±1.49,5.12±1.25 vs 3.75±1.04;6.62±2.13,6.00±1.51,5.50±1.41 vs 3.62±1.69,P<0.01或P<0.05);健脾活血方高剂量组MLH1表达与胃复春组相比明显升高(6.50±0.93 vs 4.88±1.25,P<0.05),中、低剂量组MLH1及高、中、低剂量组MSH2表达与胃复春组比较差异无统计学意义(P>0.05).结论:健脾活血方可通过降低CD44V6表达,上调MLH1、MSH2表达,减少细胞的非正常侵袭和转移,增强基因的错配修复功能,减少细胞的异常增殖和分化,发挥对大鼠胃癌前病变的治疗作用.展开更多
采用高传能线密度(LET)重离子辐照人胃癌SGC7901细胞,应用流式细胞技术、蛋白质印迹法(Western blot)及反转录聚合酶链式反应(RT-PCR)观察重离子诱导人胃癌SGC7901细胞周期、凋亡和MSH2表达状况。结果表明:与对照组相比,SGC7901细胞在...采用高传能线密度(LET)重离子辐照人胃癌SGC7901细胞,应用流式细胞技术、蛋白质印迹法(Western blot)及反转录聚合酶链式反应(RT-PCR)观察重离子诱导人胃癌SGC7901细胞周期、凋亡和MSH2表达状况。结果表明:与对照组相比,SGC7901细胞在辐射后72 h G2/M期所占细胞比率(33.26±0.08)和凋亡率(24.16±0.64)均达到峰值,且呈时间依赖性增加;经重离子照射后,DNA错配修复基因MSH2 m RNA和蛋白表达水平在6 h最高。结果提示:重离子在体外诱导SGC7901细胞周期阻滞和凋亡,且具有显著的时间依赖性效应;重离子在一定剂量和时间下,诱导了SGC7901细胞MSH2基因表达。DNA错配修复基因MSH2可能参与了重离子辐照诱导胃癌细胞DNA损伤的修复应答。展开更多
BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unkn...BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unknown.AIM To explore potential molecular connections between H.pylori infection and T2DM.METHODS We extracted gene expression arrays from three online datasets(GSE60427,GSE27411 and GSE115601).Differentially expressed genes(DEGs)commonly present in patients with H.pylori infection and T2DM were identified.Hub genes were validated using human gastric biopsy samples.Correlations between hub genes and immune cell infiltration,miRNAs,and transcription factors(TFs)were further analyzed.RESULTS A total of 67 DEGs were commonly presented in patients with H.pylori infection and T2DM.Five significantly upregulated hub genes,including TLR4,ITGAM,C5AR1,FCER1G,and FCGR2A,were finally identified,all of which are closely related to immune cell infiltration.The gene-miRNA analysis detected 13 miRNAs with at least two gene cross-links.TF-gene interaction networks showed that TLR4 was coregulated by 26 TFs,the largest number of TFs among the 5 hub genes.CONCLUSION We identified five hub genes that may have molecular connections between H.pylori infection and T2DM.This study provides new insights into the pathogenesis of H.pylori-induced onset of T2DM.展开更多
Light is an essential environmental signal perceived by a broad range of photoreceptors in plants. Among them, the red/far-red light receptor phytochromes function to promote photomorphogenesis, which is critical to t...Light is an essential environmental signal perceived by a broad range of photoreceptors in plants. Among them, the red/far-red light receptor phytochromes function to promote photomorphogenesis, which is critical to the survival of seedlings after seeds germination. The basic-helix-loop-helix transcription factors phytochrome-interacting factors (PIFs) are the pivotal direct downstream components of phytochromes. H2A.Z is a highly conserved histone variant regulating gene transcription, and its incorporation into nucleosomes is catalyzed by SWI2/SNF2-related 1 complex, in which SWI2/SNF2-related 1 complex subunit 6 (SWC6) and actin-related protein 6 (ARP6) serve as core subunits. Here, we show that PIFs physically interact with SWC6 in vitro and in vivo, leading to the disassociation of HY5 from SWC6. SWC6 and ARP6 regulate hypocotyl elongation partly through PIFs in red light. PIFs and SWC6 coregulate the expression of auxin-responsive genes such as IAA6, IAA19, IAA20, and IAA29 and repress H2A.Z deposition at IAA6 and IAA19 in red light. Based on previous studies and our findings, we propose that PIFs inhibit photomorphogenesis, at least in part, through repression of H2A.Z deposition at auxin-responsive genes mediated by the interactions of PIFs with SWC6 and promotion of their expression in red light.展开更多
文摘目的:通过观察健脾活血方对胃癌前病变大鼠胃黏膜组织中CD44V6、MLH1及MSH2表达的影响,探讨健脾活血方对其干预的作用机制.方法:除正常组外,其他大鼠采用以N-甲基-N-硝基-N-亚硝基胍(N-methyl-N-nitro N-nitrosoguanidine,MNNG)为主同时配合0.3g/L雷尼替丁、400 mL/L乙醇及饥饱失常的多因素造模法建立胃癌前病变动物模型.将造模成功的40只大鼠随机分为模型组(0.9%氯化钠溶液)、胃复春组(0.86 g/kg)、健脾活血方高、中、低剂量组(32、16、8 g/kg),每组8只,每组每天给予等量(10 mL/kg)的不同药物灌胃一次,连续10 wk.实验末处死大鼠,给予相应处理后,快速免疫组织化学检测CD44V6、MLH1及MSH2表达情况.结果:模型组CD44V6表达与正常组相比明显升高(5.12±1.96 vs 0.25±0.46,P<0.01);健脾活血方高、中剂量组CD44V6表达与模型组相比均明显降低(2.25±0.71,3.25±0.31vs 5.12±1.96,P<0.01或P<0.05),低剂量组C D44V6表达与模型组比较差异无统计学意义(P>0.05);健脾活血方高剂量组CD44V6表达与胃复春组相比明显降低(2.25±0.71 vs4.62±1.19,P<0.01),中、低剂量组CD44V6表达与胃复春组比较差异无统计学意义(P>0.05).模型组MLH1、MSH2表达与正常组相比均明显降低(3.75±1.04 vs 8.00±0.926;3.62±1.69 vs 7.25±2.12,P<0.01);健脾活血方高、中、低剂量组MLH1、MSH2表达与模型组相比均明显升高(6.50±0.93,5.25±1.49,5.12±1.25 vs 3.75±1.04;6.62±2.13,6.00±1.51,5.50±1.41 vs 3.62±1.69,P<0.01或P<0.05);健脾活血方高剂量组MLH1表达与胃复春组相比明显升高(6.50±0.93 vs 4.88±1.25,P<0.05),中、低剂量组MLH1及高、中、低剂量组MSH2表达与胃复春组比较差异无统计学意义(P>0.05).结论:健脾活血方可通过降低CD44V6表达,上调MLH1、MSH2表达,减少细胞的非正常侵袭和转移,增强基因的错配修复功能,减少细胞的异常增殖和分化,发挥对大鼠胃癌前病变的治疗作用.
文摘采用高传能线密度(LET)重离子辐照人胃癌SGC7901细胞,应用流式细胞技术、蛋白质印迹法(Western blot)及反转录聚合酶链式反应(RT-PCR)观察重离子诱导人胃癌SGC7901细胞周期、凋亡和MSH2表达状况。结果表明:与对照组相比,SGC7901细胞在辐射后72 h G2/M期所占细胞比率(33.26±0.08)和凋亡率(24.16±0.64)均达到峰值,且呈时间依赖性增加;经重离子照射后,DNA错配修复基因MSH2 m RNA和蛋白表达水平在6 h最高。结果提示:重离子在体外诱导SGC7901细胞周期阻滞和凋亡,且具有显著的时间依赖性效应;重离子在一定剂量和时间下,诱导了SGC7901细胞MSH2基因表达。DNA错配修复基因MSH2可能参与了重离子辐照诱导胃癌细胞DNA损伤的修复应答。
基金Supported by National Natural Science Foundation of China,No.82100594.
文摘BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unknown.AIM To explore potential molecular connections between H.pylori infection and T2DM.METHODS We extracted gene expression arrays from three online datasets(GSE60427,GSE27411 and GSE115601).Differentially expressed genes(DEGs)commonly present in patients with H.pylori infection and T2DM were identified.Hub genes were validated using human gastric biopsy samples.Correlations between hub genes and immune cell infiltration,miRNAs,and transcription factors(TFs)were further analyzed.RESULTS A total of 67 DEGs were commonly presented in patients with H.pylori infection and T2DM.Five significantly upregulated hub genes,including TLR4,ITGAM,C5AR1,FCER1G,and FCGR2A,were finally identified,all of which are closely related to immune cell infiltration.The gene-miRNA analysis detected 13 miRNAs with at least two gene cross-links.TF-gene interaction networks showed that TLR4 was coregulated by 26 TFs,the largest number of TFs among the 5 hub genes.CONCLUSION We identified five hub genes that may have molecular connections between H.pylori infection and T2DM.This study provides new insights into the pathogenesis of H.pylori-induced onset of T2DM.
基金This work was supported by the National Natural Science Foundation of China(31900609)the National Key Research and Development Program of China(2017YFA0503802)+1 种基金the National Natural Science Foundation of China(31530085,31900207,and 32000183)the Science and Technology Commission of Shanghai Municipality(18DZ2260500).
文摘Light is an essential environmental signal perceived by a broad range of photoreceptors in plants. Among them, the red/far-red light receptor phytochromes function to promote photomorphogenesis, which is critical to the survival of seedlings after seeds germination. The basic-helix-loop-helix transcription factors phytochrome-interacting factors (PIFs) are the pivotal direct downstream components of phytochromes. H2A.Z is a highly conserved histone variant regulating gene transcription, and its incorporation into nucleosomes is catalyzed by SWI2/SNF2-related 1 complex, in which SWI2/SNF2-related 1 complex subunit 6 (SWC6) and actin-related protein 6 (ARP6) serve as core subunits. Here, we show that PIFs physically interact with SWC6 in vitro and in vivo, leading to the disassociation of HY5 from SWC6. SWC6 and ARP6 regulate hypocotyl elongation partly through PIFs in red light. PIFs and SWC6 coregulate the expression of auxin-responsive genes such as IAA6, IAA19, IAA20, and IAA29 and repress H2A.Z deposition at IAA6 and IAA19 in red light. Based on previous studies and our findings, we propose that PIFs inhibit photomorphogenesis, at least in part, through repression of H2A.Z deposition at auxin-responsive genes mediated by the interactions of PIFs with SWC6 and promotion of their expression in red light.
