Magnesium(Mg)-based composites are expected to be useful for biodegradable bone-implant materials due to their degradability,similar elastic modulus to that of bone,and biofunctionalities.However,their rapid degradati...Magnesium(Mg)-based composites are expected to be useful for biodegradable bone-implant materials due to their degradability,similar elastic modulus to that of bone,and biofunctionalities.However,their rapid degradation,poor biotribology performance,and lack of vascularization and antibacterial activity are not conducive to bone-fixation applications.In this study,an in situ Mg_(2)Ge/Mg-Cu-P composite with a nominal composition of Mg-10Ge-2Cu-0.5P(denoted MGCP)was prepared via phosphorus(P)-modified casting followed by hot extrusion for biodegradable bone-fixation applications.For comparison,an in situ Mg_(2)Ge/Mg-Cu composite(Mg-10Ge-2Cu,denoted MGC)was prepared under the same conditions without P-modification.The hot-extruded(HE)MGCP sample showed significantly improved corrosion resistance with corrosion rates of 2.2 mm/y and 2.51 mm/y as measured by potentiodynamic-polarization and hydrogen-release testing in Dulbecco’s Modified Eagle Medium supplemented with fetal bovine serum(denoted DMEM).The HE MGCP also exhibited notably enhanced mechanical properties and biotribological resistance in DMEM,with an σ_(UTS) of ~304.2 MPa,σ_(TYS) of ~202.5 MPa,elongation of ~12.3%,σ_(UCS) of 769.0 MPa,σ_(CYS) of 208.0 MPa,and Brinell hardness of 105.3 HB,along with smallerσ_(TYS) andσ_(CYS) decreases after 3 d of immersion in Hanks’solution.In comparison to pure titanium and Mg,the HE MGCP demonstrated much greater cytocompatibility,angiogenic capacity,and osteogenic differentiation and mineralization capability.Furthermore,the HE MGCP displayed markedly higher in vitro antibacterial activity,in vivo antibacterial and anti-inflammatory ability,and good biosafety in a rat subcutaneous-implantation model compared to pure titanium and Mg,indicating significant potential for biodegradable bone-fixation applications.展开更多
目的 评估BC02佐剂配伍gE蛋白在老年小鼠模型中的免疫效性能,为优化老年群体带状疱疹疫苗设计提供实验依据。方法 将青年(6~8周龄)和老年(13月龄)BALB/c小鼠随机分为PBS组、gE+AS01B组和gE+BC02组(n=6/组),间隔4周,经大腿肌肉注射免疫2...目的 评估BC02佐剂配伍gE蛋白在老年小鼠模型中的免疫效性能,为优化老年群体带状疱疹疫苗设计提供实验依据。方法 将青年(6~8周龄)和老年(13月龄)BALB/c小鼠随机分为PBS组、gE+AS01B组和gE+BC02组(n=6/组),间隔4周,经大腿肌肉注射免疫2次(间隔4周)疫苗。采用ELISA检测血清gE特异性IgG及其亚类抗体水平,ELISpot分析脾淋巴细胞IFN-γ、IL-2和IL-4分泌能力,流式细胞术检测CD4^(+)T细胞亚群(IFN-γ^(+)、IL-2^(+)、IL-4^(+)、IL-17+)及记忆T细胞(IFN-γ^(+)、IL-2^(+))比例。结果 gE+BC02组在青年和老年小鼠中均诱导了显著升高的gE特异性IgG、IgG1和IgG2a抗体,且抗体水平无年龄差异。细胞免疫应答方面,老年小鼠中,gE+AS01B组诱导的IFN-γ^(+)CD4^(+)(0.188±0.08 vs 0.086±0.06)和IL-2^(+)CD4^(+)(0.448±0.07 vs 0.338±0.07)T细胞比例为gE+BC02疫苗的2.19和1.33倍;而青年小鼠gE+BC02组IL-17+CD4^(+)(0.144±0.03 vs 0.092±0.023)T细胞比例则为gE+AS01B组的1.57倍。记忆T细胞应答分析显示,gE+BC02组老年小鼠IFN-γ^(+)和IL-2^(+)记忆T细胞比例较青年组显著降低53.94%(0.456±0.14 vs 0.99±0.37)和41.83%(0.356±0.12 vs 0.612±0.24),其余无显著性差异。结论 BC02佐剂可有效激活老年小鼠的体液免疫应答,但其诱导的Th1型细胞免疫应答稍弱于AS01B佐剂,且均存在年龄相关差异。本研究为开发适合老年人群的带状疱疹疫苗提供了重要实验依据,提示未来可通过优化BC02佐剂配方来增强Th1型免疫应答。展开更多
在^(76)Ge同位素生产工艺中,电动调节阀是通过DCS(Distributed Control System)的PID模块来控制,从而实现级联管线内部压力的自动调节;但当工艺系统出现较大压力波动时,现有的电动调节阀控制方法,无法达到最优的控制效果,导致被控工艺...在^(76)Ge同位素生产工艺中,电动调节阀是通过DCS(Distributed Control System)的PID模块来控制,从而实现级联管线内部压力的自动调节;但当工艺系统出现较大压力波动时,现有的电动调节阀控制方法,无法达到最优的控制效果,导致被控工艺压力超出运行安全限值,对工艺系统的安全稳定运行产生了严重不良影响。本研究针对目前电动调节阀在压力异常情况下控制性能较弱问题,提出了对电动调节阀的DCS控制组态程序进行优化的控制方案。实际运行表明,优化后的控制程序避免了异常情况下工艺压力出现较大波动的现象,有力地保障了^(76)Ge同位素生产线的安全平稳运行。展开更多
基金supported by the Zhejiang Public Welfare Technology Application Research Project of China(No.LTGY24H140002,No.LTGY23H140002,No.LGF22H140008)Wenzhou Medical University Basic Scientific Research Expenses Project(KYYW202331)the financial support for this research by the Australian Research Council(ARC)through the Discovery Project(DP240101131).
文摘Magnesium(Mg)-based composites are expected to be useful for biodegradable bone-implant materials due to their degradability,similar elastic modulus to that of bone,and biofunctionalities.However,their rapid degradation,poor biotribology performance,and lack of vascularization and antibacterial activity are not conducive to bone-fixation applications.In this study,an in situ Mg_(2)Ge/Mg-Cu-P composite with a nominal composition of Mg-10Ge-2Cu-0.5P(denoted MGCP)was prepared via phosphorus(P)-modified casting followed by hot extrusion for biodegradable bone-fixation applications.For comparison,an in situ Mg_(2)Ge/Mg-Cu composite(Mg-10Ge-2Cu,denoted MGC)was prepared under the same conditions without P-modification.The hot-extruded(HE)MGCP sample showed significantly improved corrosion resistance with corrosion rates of 2.2 mm/y and 2.51 mm/y as measured by potentiodynamic-polarization and hydrogen-release testing in Dulbecco’s Modified Eagle Medium supplemented with fetal bovine serum(denoted DMEM).The HE MGCP also exhibited notably enhanced mechanical properties and biotribological resistance in DMEM,with an σ_(UTS) of ~304.2 MPa,σ_(TYS) of ~202.5 MPa,elongation of ~12.3%,σ_(UCS) of 769.0 MPa,σ_(CYS) of 208.0 MPa,and Brinell hardness of 105.3 HB,along with smallerσ_(TYS) andσ_(CYS) decreases after 3 d of immersion in Hanks’solution.In comparison to pure titanium and Mg,the HE MGCP demonstrated much greater cytocompatibility,angiogenic capacity,and osteogenic differentiation and mineralization capability.Furthermore,the HE MGCP displayed markedly higher in vitro antibacterial activity,in vivo antibacterial and anti-inflammatory ability,and good biosafety in a rat subcutaneous-implantation model compared to pure titanium and Mg,indicating significant potential for biodegradable bone-fixation applications.
文摘目的 评估BC02佐剂配伍gE蛋白在老年小鼠模型中的免疫效性能,为优化老年群体带状疱疹疫苗设计提供实验依据。方法 将青年(6~8周龄)和老年(13月龄)BALB/c小鼠随机分为PBS组、gE+AS01B组和gE+BC02组(n=6/组),间隔4周,经大腿肌肉注射免疫2次(间隔4周)疫苗。采用ELISA检测血清gE特异性IgG及其亚类抗体水平,ELISpot分析脾淋巴细胞IFN-γ、IL-2和IL-4分泌能力,流式细胞术检测CD4^(+)T细胞亚群(IFN-γ^(+)、IL-2^(+)、IL-4^(+)、IL-17+)及记忆T细胞(IFN-γ^(+)、IL-2^(+))比例。结果 gE+BC02组在青年和老年小鼠中均诱导了显著升高的gE特异性IgG、IgG1和IgG2a抗体,且抗体水平无年龄差异。细胞免疫应答方面,老年小鼠中,gE+AS01B组诱导的IFN-γ^(+)CD4^(+)(0.188±0.08 vs 0.086±0.06)和IL-2^(+)CD4^(+)(0.448±0.07 vs 0.338±0.07)T细胞比例为gE+BC02疫苗的2.19和1.33倍;而青年小鼠gE+BC02组IL-17+CD4^(+)(0.144±0.03 vs 0.092±0.023)T细胞比例则为gE+AS01B组的1.57倍。记忆T细胞应答分析显示,gE+BC02组老年小鼠IFN-γ^(+)和IL-2^(+)记忆T细胞比例较青年组显著降低53.94%(0.456±0.14 vs 0.99±0.37)和41.83%(0.356±0.12 vs 0.612±0.24),其余无显著性差异。结论 BC02佐剂可有效激活老年小鼠的体液免疫应答,但其诱导的Th1型细胞免疫应答稍弱于AS01B佐剂,且均存在年龄相关差异。本研究为开发适合老年人群的带状疱疹疫苗提供了重要实验依据,提示未来可通过优化BC02佐剂配方来增强Th1型免疫应答。
文摘在^(76)Ge同位素生产工艺中,电动调节阀是通过DCS(Distributed Control System)的PID模块来控制,从而实现级联管线内部压力的自动调节;但当工艺系统出现较大压力波动时,现有的电动调节阀控制方法,无法达到最优的控制效果,导致被控工艺压力超出运行安全限值,对工艺系统的安全稳定运行产生了严重不良影响。本研究针对目前电动调节阀在压力异常情况下控制性能较弱问题,提出了对电动调节阀的DCS控制组态程序进行优化的控制方案。实际运行表明,优化后的控制程序避免了异常情况下工艺压力出现较大波动的现象,有力地保障了^(76)Ge同位素生产线的安全平稳运行。
