Background： Current research to enrich cattle feed has primarily focused on treatment using white rot fungi, while there are scarce reports using the enzyme tannase, which is discussed only in reviews or in the form ...Background： Current research to enrich cattle feed has primarily focused on treatment using white rot fungi, while there are scarce reports using the enzyme tannase, which is discussed only in reviews or in the form of a hypothesis. In this context, the aim of the present study was to evaluate the effect of tannase on wheat straw （WS） and also the effect of lyophilized tannase at concentrations of 0.1%, 0.2%, and 0.3% （w/w） on WS followed by fermentation with Ganodermo sp. for 10 d and compared in relation to biochemical parameters, crude protein （CP） content, and nutritional value by calculating the C/N ratio in order to improve the nutritional value of cattle feed. Results： Penicillium charlesii, a tannase-producing microorganism, produced 61.4 IU/mL of tannase in 54 h when 2% （w/v） tannic acid （TA） was initially used as a substrate in medium containing （% w/v） sucrose （1.0）, NaNO3 （1.0）, and MgSO4 （0.08 pH, 5.0） in a 300-L fermentor （working volume 220 L）, and concomitantly fed with 1.0% （w/v） TA after 24 h. The yield of partially purified and lyophilized tannase was 5.8 IU/mg. The tannin-free myco-straw at 0.1% （w/w） tannase showed 37.8% （w/w） lignin degradation with only a 20.4% （w/w） decrease in cellulose content and the in vitro feed digestibility was 32.2%. An increase in CP content （up to 1.28-fold） along with a lower C/N ratio of 25.0%, as compared to myco-straw, was obtained. Conclusions： The use of tannin-free myco-straw has potential to improve the nutritional content of cattle feed. This biological treatment process was safe, eco-friendly, easy to perform, and was less expensive as compared to other treatment methods.展开更多
基金supported by a grant from the Council of Scientific and Industrial Research of India to S.R.(grant no.:9/45(1190)/2012-EMR-1)
文摘Background： Current research to enrich cattle feed has primarily focused on treatment using white rot fungi, while there are scarce reports using the enzyme tannase, which is discussed only in reviews or in the form of a hypothesis. In this context, the aim of the present study was to evaluate the effect of tannase on wheat straw （WS） and also the effect of lyophilized tannase at concentrations of 0.1%, 0.2%, and 0.3% （w/w） on WS followed by fermentation with Ganodermo sp. for 10 d and compared in relation to biochemical parameters, crude protein （CP） content, and nutritional value by calculating the C/N ratio in order to improve the nutritional value of cattle feed. Results： Penicillium charlesii, a tannase-producing microorganism, produced 61.4 IU/mL of tannase in 54 h when 2% （w/v） tannic acid （TA） was initially used as a substrate in medium containing （% w/v） sucrose （1.0）, NaNO3 （1.0）, and MgSO4 （0.08 pH, 5.0） in a 300-L fermentor （working volume 220 L）, and concomitantly fed with 1.0% （w/v） TA after 24 h. The yield of partially purified and lyophilized tannase was 5.8 IU/mg. The tannin-free myco-straw at 0.1% （w/w） tannase showed 37.8% （w/w） lignin degradation with only a 20.4% （w/w） decrease in cellulose content and the in vitro feed digestibility was 32.2%. An increase in CP content （up to 1.28-fold） along with a lower C/N ratio of 25.0%, as compared to myco-straw, was obtained. Conclusions： The use of tannin-free myco-straw has potential to improve the nutritional content of cattle feed. This biological treatment process was safe, eco-friendly, easy to perform, and was less expensive as compared to other treatment methods.
