A new alkaloid,sinensine(1),had been isolated from the fruiting bodies of Ganoderma sinense Zhao,Xu et Zhang.Its structure was elucidated on the basis of 1D and 2D spectral analysis.This alkaloid exhibited activity in...A new alkaloid,sinensine(1),had been isolated from the fruiting bodies of Ganoderma sinense Zhao,Xu et Zhang.Its structure was elucidated on the basis of 1D and 2D spectral analysis.This alkaloid exhibited activity in protecting the injury induced by hydrogen peroxide oxidation on HUVEC,with EC_(50) value 6.2μmol/L.展开更多
Four new alkaloids,sinensines B-E(1-4),together with one known alkaloid,sinensine(5),were isolated from the fruiting bodies of Ganoderma sinense.Their structures were elucidated on the basis of 1D and 2D NMR spectra a...Four new alkaloids,sinensines B-E(1-4),together with one known alkaloid,sinensine(5),were isolated from the fruiting bodies of Ganoderma sinense.Their structures were elucidated on the basis of 1D and 2D NMR spectra analysis.The structure of sinensine E was confirmed by X-ray crystallographic analysis of its acetyl product(4a).展开更多
Ganoderma sinense is a traditional and protein-rich edible fungus with outstanding immunoregulatory activity.However,the mechanism through which G.sinense protein hydrolysates and peptides exert their immunomodulatory...Ganoderma sinense is a traditional and protein-rich edible fungus with outstanding immunoregulatory activity.However,the mechanism through which G.sinense protein hydrolysates and peptides exert their immunomodulatory effects is unclear.The objective of this study was to prepare and isolate immunologically active peptides from G.sinense protein hydrolysates(GSP) and to investigate their impact on the activation of macrophages.G.sinense peptides with low molecular weights(< 1 kDa,87.76%) markedly promoted RAW264.7 cell proliferation;increased their phagocytic capacity,nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin-10(IL-10),and IL-6 secretion and reactive oxygen species(ROS) production;and upregulated Tlr2 mRNA expression.In addition,GSP promoted nuclear factor kappa-B(NF-κB) activation and translocation through the upregulation of p65 and p-p65 protein expression.Virtual molecular screening technology revealed that compared with other peptides,the SFAGNIPVNR,YGDAFIR and TVSYLPAPQR peptides derived from GSP showed stronger binding affinities.Interaction site map analysis indicated that the SFAGNIPVNR and YGDAFIR peptides formed stable hydrogen bonds with toll-like receptor 2(TLR2).Together,these results suggested that G.sinense peptides can serve as important ingredients in nutraceuticals or functional foods.展开更多
The water-soluble part（GS） of Ganoderma sinense Zhao, Xu et Zhang was divided into high molecular（GS-H） and low molecular（GS-L） parts by Cellulose Super Filtration, and GS was also fractionated into four fractio...The water-soluble part（GS） of Ganoderma sinense Zhao, Xu et Zhang was divided into high molecular（GS-H） and low molecular（GS-L） parts by Cellulose Super Filtration, and GS was also fractionated into four fractions, GS-1, 2, 3, and 4 by ethanol precipitation according to their molecular weights. Chemical analysis shows that GS and GS-1, 2, 3, 4 were complexes of polysaccharide and peptide. The fractions with molecular weights over 4000, GS-1, 2, 3, and GS-H show anti-tumor activities, however, the fractions with molecular weights lower than 4000, GS-4, and GS-L have no anti-tumor activity, indicating that the anti-tumor activity of Ganoderma Sinensis was caused by glucopeptides with molecular weight ranging from 4000 to 20000. Two purified glucopeptides, GS-6b and GS-7b were obtained from GS-H by ion-exchange and gel-permeation chromatography. Their molecular weights, glycosidic linkages, and configurations were detected by means of IR spectrum, sugar composition analysis, and methylation analysis. The polysaccharide parts of GS-6b and GS-7b had glucan backbone consisting of β-1→3 Glc, and side chain containing glucosyl, mannosyl, fucosyl, xylosyl, galactosyl, and glucuronic acid residues attached on 1-2, 1-4, 1-6 positions of the backbone of GS-6b, or 1-6, 1-4 positions of the backbone of GS-7b. The peptide parts in GS-6b and GS-7b were composed of 10 kinds of amino acids, including Asp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Met, and Lys.展开更多
基金supported by the Ministry of Science and Technology of the People's Republic of China (No.2007Fy130100)
文摘A new alkaloid,sinensine(1),had been isolated from the fruiting bodies of Ganoderma sinense Zhao,Xu et Zhang.Its structure was elucidated on the basis of 1D and 2D spectral analysis.This alkaloid exhibited activity in protecting the injury induced by hydrogen peroxide oxidation on HUVEC,with EC_(50) value 6.2μmol/L.
基金The project was financially supported by the General Program of NSFC(No.81172940)Knowledge Innovation Program of the CAS(Grant No.KSCX2-YW-G-038,KSCX2-YW-R-194,KZCX2-XB2-15-03,)as well as Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2010-ZZ14).
文摘Four new alkaloids,sinensines B-E(1-4),together with one known alkaloid,sinensine(5),were isolated from the fruiting bodies of Ganoderma sinense.Their structures were elucidated on the basis of 1D and 2D NMR spectra analysis.The structure of sinensine E was confirmed by X-ray crystallographic analysis of its acetyl product(4a).
基金supported by the National Natural Science Foundation,China(32402067)the Startup Fund for Scientific Research,Fujian Medical University(XRCZX2020032)the Natural Science Foundation of Fujian Province,China(2023J01307)。
文摘Ganoderma sinense is a traditional and protein-rich edible fungus with outstanding immunoregulatory activity.However,the mechanism through which G.sinense protein hydrolysates and peptides exert their immunomodulatory effects is unclear.The objective of this study was to prepare and isolate immunologically active peptides from G.sinense protein hydrolysates(GSP) and to investigate their impact on the activation of macrophages.G.sinense peptides with low molecular weights(< 1 kDa,87.76%) markedly promoted RAW264.7 cell proliferation;increased their phagocytic capacity,nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin-10(IL-10),and IL-6 secretion and reactive oxygen species(ROS) production;and upregulated Tlr2 mRNA expression.In addition,GSP promoted nuclear factor kappa-B(NF-κB) activation and translocation through the upregulation of p65 and p-p65 protein expression.Virtual molecular screening technology revealed that compared with other peptides,the SFAGNIPVNR,YGDAFIR and TVSYLPAPQR peptides derived from GSP showed stronger binding affinities.Interaction site map analysis indicated that the SFAGNIPVNR and YGDAFIR peptides formed stable hydrogen bonds with toll-like receptor 2(TLR2).Together,these results suggested that G.sinense peptides can serve as important ingredients in nutraceuticals or functional foods.
文摘The water-soluble part（GS） of Ganoderma sinense Zhao, Xu et Zhang was divided into high molecular（GS-H） and low molecular（GS-L） parts by Cellulose Super Filtration, and GS was also fractionated into four fractions, GS-1, 2, 3, and 4 by ethanol precipitation according to their molecular weights. Chemical analysis shows that GS and GS-1, 2, 3, 4 were complexes of polysaccharide and peptide. The fractions with molecular weights over 4000, GS-1, 2, 3, and GS-H show anti-tumor activities, however, the fractions with molecular weights lower than 4000, GS-4, and GS-L have no anti-tumor activity, indicating that the anti-tumor activity of Ganoderma Sinensis was caused by glucopeptides with molecular weight ranging from 4000 to 20000. Two purified glucopeptides, GS-6b and GS-7b were obtained from GS-H by ion-exchange and gel-permeation chromatography. Their molecular weights, glycosidic linkages, and configurations were detected by means of IR spectrum, sugar composition analysis, and methylation analysis. The polysaccharide parts of GS-6b and GS-7b had glucan backbone consisting of β-1→3 Glc, and side chain containing glucosyl, mannosyl, fucosyl, xylosyl, galactosyl, and glucuronic acid residues attached on 1-2, 1-4, 1-6 positions of the backbone of GS-6b, or 1-6, 1-4 positions of the backbone of GS-7b. The peptide parts in GS-6b and GS-7b were composed of 10 kinds of amino acids, including Asp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Met, and Lys.
