Objective To study the chemical constituents of the fungus Ganoderma cochlear. Methods The compounds were isolated by using MCI gel CHP 20P, Sephadex LH-20, RP-18 column chromatography, and preparative TLC. The struct...Objective To study the chemical constituents of the fungus Ganoderma cochlear. Methods The compounds were isolated by using MCI gel CHP 20P, Sephadex LH-20, RP-18 column chromatography, and preparative TLC. The structures were identified by means of spectroscopic methods. Results Two phenolic normeroterpenoid and meroterpenoid, cochlearols C and D （1 and 2）, together with six benzene derivatives, 3- methoxy-4-hydroxy-phenylethanol （3）, 4-hydroxyacetophenone （4）, p-hydroxycinnamic methyl ester （5）, 2-methoxy-4-hydroxybenzaldehyde （6）, 4-hydroxy-3-methoxy benzoic acid （7）, and 2-hydroxy-5-ethoxybenzoic acid （8）, were isolated from the fruiting bodies of Ganoderma cochlear. Conclusion Compounds 1 and 2 are new phenolic normeroterpenoid and meroterpenoid, respectively.展开更多
Four new alkaloids,sinensines B-E(1-4),together with one known alkaloid,sinensine(5),were isolated from the fruiting bodies of Ganoderma sinense.Their structures were elucidated on the basis of 1D and 2D NMR spectra a...Four new alkaloids,sinensines B-E(1-4),together with one known alkaloid,sinensine(5),were isolated from the fruiting bodies of Ganoderma sinense.Their structures were elucidated on the basis of 1D and 2D NMR spectra analysis.The structure of sinensine E was confirmed by X-ray crystallographic analysis of its acetyl product(4a).展开更多
为探讨海南灵芝(Ganoderma hainanense Zhao,Xu et Zhang)的化学成分,采用柱层析技术从海南灵芝乙醇提取物中分离得到14个化合物。经波谱分析鉴定其结构分别为:巴西红厚壳素(1)、6-脱氧巴西红厚壳素(2)、7,8-二甲基咯嗪(3)、5,8-过氧麦...为探讨海南灵芝(Ganoderma hainanense Zhao,Xu et Zhang)的化学成分,采用柱层析技术从海南灵芝乙醇提取物中分离得到14个化合物。经波谱分析鉴定其结构分别为:巴西红厚壳素(1)、6-脱氧巴西红厚壳素(2)、7,8-二甲基咯嗪(3)、5,8-过氧麦角固醇(4)、3β,5α,9α-三羟基麦角甾-7,22-二烯-6-酮(5)、麦角甾-7,22-二烯-3β-醇(6)、麦角甾-7,22-二烯-3-酮(7)、7α-甲氧基-5α,6α-环氧麦角甾-8(14),22-二烯-3β-醇(8)、麦角甾-7,22-二烯-3β,5α-6β-三醇(9)、3-吲哚甲酸(10)、3,4-二羟基苯甲酸(11)、对羟基苯甲酸(12)、3,4-二羟基苯甲酸甲酯(13)和正二十六烷酸(14)。所有化合物均为首次从海南灵芝中分离得到。展开更多
从海南岛采集到7种野生灵芝,根据形态和r DNA ITS序列的系统发育分析,将其鉴定为树舌灵芝Ganoderma applanatum、南方灵芝G.australe、布朗灵芝G.brownii、桂兰灵芝G.guinanense、无柄紫灵芝G.mastoporum、灵芝G.sichuanense和紫芝G.sin...从海南岛采集到7种野生灵芝,根据形态和r DNA ITS序列的系统发育分析,将其鉴定为树舌灵芝Ganoderma applanatum、南方灵芝G.australe、布朗灵芝G.brownii、桂兰灵芝G.guinanense、无柄紫灵芝G.mastoporum、灵芝G.sichuanense和紫芝G.sinense。本文提供了7种灵芝的形态描述和宏观图17条分子序列,并结合genbank中灵芝属的分子序列构建了分子系统发育树,为准确的保护和利用海南岛及我国的野生灵芝资源提供了依据。展开更多
“Lingzhi”is a mushroom that has been renowned in China for more than 2,000 years because of its claimed medicinal properties plus its symbolic fortune.“Lingzhi”has high economic value mostly as a dietary supplemen...“Lingzhi”is a mushroom that has been renowned in China for more than 2,000 years because of its claimed medicinal properties plus its symbolic fortune.“Lingzhi”has high economic value mostly as a dietary supplement in the modern market especially in East Asia,and its medicinal functions have become a hot study topic.For over a century,the highly prized medicinal fungus,known as“Lingzhi”in East Asia,has been assigned to Ganoderma lucidum,a species originally described from Europe.Molecular studies in recent years have revealed that the commercially cultivated‘G.lucidum’(“Lingzhi”)in East Asia is a different species from the true G.lucidum.The present study aims to clarify the species identity of“Lingzhi”based on morphological studies and analysis of rDNA nuc-ITS sequences,and additional gene fragments of mt-SSU,RPB1,RPB2,and TEF1-αof“Lingzhi”were provided.All Ganoderma species that mostly resemble“Lingzhi”in phylogeny and/or morphology were included for analysis.We propose a new species G.lingzhi for“Lingzhi”,which has an East Asia distribution.The most striking characteristics which differentiate G.lingzhi from G.lucidum are the presence of melanoid bands in the context,a yellow pore surface and thick dissepiments(80–120μm)at maturity.G.curtisii is most closely related to G.lingzhi in phylogeny and is from North America.Ganoderma flexipes,G.multipileum,G.sichuanense,G.tropicum and‘G.tsugae’,are also closely related with G.lingzhi and are reported from China.These species are compared and discussed.‘Ganoderma tsuage’reported from China is determined as conspecific with G.lucidum,hence the distribution of G.lucidum extends from Europe to northeastern China.展开更多
基金NSFC-Joint Foundation of Yunnan Province(U1202222)National Natural Science Foundation of China(21472199)+1 种基金a project from State Key Laboratory of Phytochemistry and Plant Resources in West ChinaKunming Institute of Botany(P2013-ZZ03)
文摘Objective To study the chemical constituents of the fungus Ganoderma cochlear. Methods The compounds were isolated by using MCI gel CHP 20P, Sephadex LH-20, RP-18 column chromatography, and preparative TLC. The structures were identified by means of spectroscopic methods. Results Two phenolic normeroterpenoid and meroterpenoid, cochlearols C and D （1 and 2）, together with six benzene derivatives, 3- methoxy-4-hydroxy-phenylethanol （3）, 4-hydroxyacetophenone （4）, p-hydroxycinnamic methyl ester （5）, 2-methoxy-4-hydroxybenzaldehyde （6）, 4-hydroxy-3-methoxy benzoic acid （7）, and 2-hydroxy-5-ethoxybenzoic acid （8）, were isolated from the fruiting bodies of Ganoderma cochlear. Conclusion Compounds 1 and 2 are new phenolic normeroterpenoid and meroterpenoid, respectively.
基金The project was financially supported by the General Program of NSFC(No.81172940)Knowledge Innovation Program of the CAS(Grant No.KSCX2-YW-G-038,KSCX2-YW-R-194,KZCX2-XB2-15-03,)as well as Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2010-ZZ14).
文摘Four new alkaloids,sinensines B-E(1-4),together with one known alkaloid,sinensine(5),were isolated from the fruiting bodies of Ganoderma sinense.Their structures were elucidated on the basis of 1D and 2D NMR spectra analysis.The structure of sinensine E was confirmed by X-ray crystallographic analysis of its acetyl product(4a).
文摘为探讨海南灵芝(Ganoderma hainanense Zhao,Xu et Zhang)的化学成分,采用柱层析技术从海南灵芝乙醇提取物中分离得到14个化合物。经波谱分析鉴定其结构分别为:巴西红厚壳素(1)、6-脱氧巴西红厚壳素(2)、7,8-二甲基咯嗪(3)、5,8-过氧麦角固醇(4)、3β,5α,9α-三羟基麦角甾-7,22-二烯-6-酮(5)、麦角甾-7,22-二烯-3β-醇(6)、麦角甾-7,22-二烯-3-酮(7)、7α-甲氧基-5α,6α-环氧麦角甾-8(14),22-二烯-3β-醇(8)、麦角甾-7,22-二烯-3β,5α-6β-三醇(9)、3-吲哚甲酸(10)、3,4-二羟基苯甲酸(11)、对羟基苯甲酸(12)、3,4-二羟基苯甲酸甲酯(13)和正二十六烷酸(14)。所有化合物均为首次从海南灵芝中分离得到。
文摘从海南岛采集到7种野生灵芝,根据形态和r DNA ITS序列的系统发育分析,将其鉴定为树舌灵芝Ganoderma applanatum、南方灵芝G.australe、布朗灵芝G.brownii、桂兰灵芝G.guinanense、无柄紫灵芝G.mastoporum、灵芝G.sichuanense和紫芝G.sinense。本文提供了7种灵芝的形态描述和宏观图17条分子序列,并结合genbank中灵芝属的分子序列构建了分子系统发育树,为准确的保护和利用海南岛及我国的野生灵芝资源提供了依据。
基金financed by the National Natural Science Foundation of China(Project Nos.30910103907,31070022).
文摘“Lingzhi”is a mushroom that has been renowned in China for more than 2,000 years because of its claimed medicinal properties plus its symbolic fortune.“Lingzhi”has high economic value mostly as a dietary supplement in the modern market especially in East Asia,and its medicinal functions have become a hot study topic.For over a century,the highly prized medicinal fungus,known as“Lingzhi”in East Asia,has been assigned to Ganoderma lucidum,a species originally described from Europe.Molecular studies in recent years have revealed that the commercially cultivated‘G.lucidum’(“Lingzhi”)in East Asia is a different species from the true G.lucidum.The present study aims to clarify the species identity of“Lingzhi”based on morphological studies and analysis of rDNA nuc-ITS sequences,and additional gene fragments of mt-SSU,RPB1,RPB2,and TEF1-αof“Lingzhi”were provided.All Ganoderma species that mostly resemble“Lingzhi”in phylogeny and/or morphology were included for analysis.We propose a new species G.lingzhi for“Lingzhi”,which has an East Asia distribution.The most striking characteristics which differentiate G.lingzhi from G.lucidum are the presence of melanoid bands in the context,a yellow pore surface and thick dissepiments(80–120μm)at maturity.G.curtisii is most closely related to G.lingzhi in phylogeny and is from North America.Ganoderma flexipes,G.multipileum,G.sichuanense,G.tropicum and‘G.tsugae’,are also closely related with G.lingzhi and are reported from China.These species are compared and discussed.‘Ganoderma tsuage’reported from China is determined as conspecific with G.lucidum,hence the distribution of G.lucidum extends from Europe to northeastern China.
