Basic conditions and new countryside construction of Ganlu Town, Zizhong County, Neijiang City were analyzed, SWOT adopted to analyze internal strengths, weaknesses, external opportunities and threats of its new count...Basic conditions and new countryside construction of Ganlu Town, Zizhong County, Neijiang City were analyzed, SWOT adopted to analyze internal strengths, weaknesses, external opportunities and threats of its new countryside construction. On this basis, positive and practical strategies were put forward to promote the new countryside construction in the local area, and provide references for the new countryside construction in other regions.展开更多
[Objectives]This study was conducted to explore the mechanism of Ganlu Yin in the treatment of COVID-19 based on network pharmacology and molecular docking technology. [Methods] TCMSP, ETCM and TCMID databases were us...[Objectives]This study was conducted to explore the mechanism of Ganlu Yin in the treatment of COVID-19 based on network pharmacology and molecular docking technology. [Methods] TCMSP, ETCM and TCMID databases were used to dig out the chemical components in the compound ingredients of Ganlu Yin. Swiss ADME platform was used for ADME screening and Swiss Target Prediction server was used to predict potential targets of chemical components. The COVID-19 related genes were obtained from GeneCards database and Drugbank website, and the intersection targets between Ganlu Yin and COVID-19 were obtained by Venn analysis. The targets were imported into String analysis platform to construct a protein interaction network, and Cytoscape 3.9.0 software was used for visualization processing. GO enrichment and KEGG pathway analysis were performed on the intersection targets through David database. The core components, clinical antiviral drugs, core targets and novel Coronavirus(SARS-COV-2) hydrolase 3 CLpro(Mpro), omicron B.1.1.529 spike protein and angiotensin converting enzyme 2(ACE2) were tested by molecular docking. [Results] A total of 213 effective chemical components and 1 023 drug targets in Ganlu Yin were screened out, including 4 620 COVID-19 related genes and 328 intersection targets. The core components mainly included β-sitosterol, quercetin, and kaherol. The key core targets involved GAPDH, AKT1, TNF, ALB, and EGFR, etc. A total of 241 items were screened by GO functional enrichment, including inflammatory response, protein phosphorylation, positive regulation of gene expression and other biological processes. A total of 85 KEGG pathways were screened out, which were mainly enriched in influenza A, HIF-1 signaling pathway, hepatitis C and other pathways. The results of molecular docking showed that the core chemical components in Ganlu Yin had good binding ability with core targets and disease targets ACE2, 3 CLpro and spike protein, especially kahenol with ACE2, β-sitosterol with 3 CLpro, and quercetin with spike protein. [Conclusions] Prescription Ganlu Yin has the characteristics of multi-component and multi-target action, and can treat COVID-19 through multiple signaling pathways.展开更多
Objective:To observe the curative effect of Modified Ganlu Xiaodu Dan and Kuhuang San combined with interferon on the treatment of HFMD of damp-heat accumulation toxin syndrome and its effects on liver function, myoca...Objective:To observe the curative effect of Modified Ganlu Xiaodu Dan and Kuhuang San combined with interferon on the treatment of HFMD of damp-heat accumulation toxin syndrome and its effects on liver function, myocardial enzymes, inflammatory factors and immunoglobulin.Merhods:From June 2018 to November 2018, 74 children with damp-heat accumulation toxin syndrome who were diagnosed with hand, foot and mouth disease have been randomly divided into the observation group (38 cases) and the control group (36 cases).The control group is given conventional Western medicine symptomatic treatment, and sprayed on the oral cavity with IFN- atomized inhalation or IFN- 2b spray. On the basis of the control group, the observation group is given Modified Ganlu Xiaodu Dan, and Kuhuangsan for washing body. 7 d is a course of treatment. The recurrence time of the two groups, the time of rash regression and the efficacy of the children have been observed. The changes of liver function, myocardial zymogram, inflammatory factors and immunoglobulin are compared before and after treatment.Results: The efficacy of the observation group was 92.1% higher than that of the control group (83.3%), and the difference was statistically significant. The time required for the body temperature of the observation group (2.64±0.57) d was lower than that of the control group (3.12±0.62) d, and the time required for the rash to resolve was (4.18±0.69) d, which was lower than that of the control group (5.25±0.73) d. Statistically significant. The levels of ALT, AST, CK-MB, IL-4 and IL-6 in the observation group were lower than those in the control group after treatment, the difference was statistically significant. The serum IgA and IgM levels in the observation group were higher than those in the control group after treatment. Conclusion: Modified Ganlu Xiaodu Dan and Kuhuang San combined with interferon for the treatment of HFMD of damp-heat accumulation toxin syndrome have a sound curative effect and it can improve liver function, protect cardiomyocytes, reduce inflammatory factors, and regulate immune function for children.展开更多
Objective: To analyze the potential active components and molecular mechanism of Ganlu Xiaodu MicroPill in the treatment of COVID-19 by network pharmacology and molecular docking. Methods: the active chemical constitu...Objective: To analyze the potential active components and molecular mechanism of Ganlu Xiaodu MicroPill in the treatment of COVID-19 by network pharmacology and molecular docking. Methods: the active chemical constituents and action targets of Ganlu Xiaodu MicroPill were searched by TCMSP and BATMAN-TCM platform, and the key targets were matched with the COVID-19 targets searched by CTD database. The core targets were selected and analyzed by GO and KEGG enrichment analysis by R software to predict the action mechanism of traditional Chinese medicine compound. The key component sets were docked with 3CLpro and ACE2. Results: 138 active components and 53 key targets were obtained. There are 1298 items in GO biological process, 59 items in cell composition, 62 items in molecular function, and 120 items enriched in KEGG pathway. Results: molecular docking showed that the affinity of 20 chemical components such as quercetin, luteolin and kaempferol with ACE2 and 3CLpro was higher than that of ribavirin and chloroquine. Conclusion: Ganlu Xiaodu MicroPill may regulate and interfere with SARS-CoV-2 infection through antivirus, inhibition of inflammatory factors and regulation of immunity, which fully reflects the therapeutic advantages of multi-components, multi-targets and multi-pathways of traditional Chinese medicine.展开更多
目的:运用网络药理学及分子对接的方法,深入探索蒙药五味甘露治疗皮肤病的关键成分和作用靶点,从而揭示其作用机制。方法:依托中药系统药理学数据库与分析平台(TCMSP)筛选五味甘露组方药材的主要化学成分与作用靶点,借助GeneCards数据...目的:运用网络药理学及分子对接的方法,深入探索蒙药五味甘露治疗皮肤病的关键成分和作用靶点,从而揭示其作用机制。方法:依托中药系统药理学数据库与分析平台(TCMSP)筛选五味甘露组方药材的主要化学成分与作用靶点,借助GeneCards数据库收集皮肤病相关关键靶点,并用Cytoscape软件建立成分-靶点作用网络,刻画出关键成分和靶点。利用String数据库对关键靶点进行PPI网络/构建及拓扑分析,得到五味甘露治疗皮肤病的主要靶点,进而作GO富集和KEGG通路富集分析。Discovery Studio 2022软件CDOCKER模块进行分子对接,表征五味甘露治疗皮肤病的生物学过程和信号通路。结果:筛选得到五味甘露1638个化学成分作用的265个皮肤病相关疾病靶点。通过成分-靶点网络构建发现五味甘露主要依靠山柰酚、亚油酸、油酸、槲皮苷等10个成分作用于IL-6、PPARG、TP53、TLR4、VEGFA等50个主要靶点发挥治疗皮肤病的作用。上述靶点主要涉及DNA转录、氧化还原酶活性、炎症反应、细胞增殖及凋亡调控、蛋白结合等生物过程。KEGG信号通路分析显示,五味甘露治疗皮肤病主要涉及C型凝集素受体信号通路、Toll样受体等信号通路。分子对接结果显示五味甘露山柰酚、亚油酸、油酸、槲皮苷等活性成分与皮肤病作用于皮肤病IL-6、PPARG、TP53、TLR4、VEGFA等因子具有良好的结合活性。结论:五味甘露通过多个重要成分作用于皮肤病关键靶点,并通过调控白细胞迁移、伤口愈合、细胞增殖及凋亡调控等多个生物过程发挥作用,本研究为今后皮肤病的研究和治疗提供了机制基础。展开更多
文摘Basic conditions and new countryside construction of Ganlu Town, Zizhong County, Neijiang City were analyzed, SWOT adopted to analyze internal strengths, weaknesses, external opportunities and threats of its new countryside construction. On this basis, positive and practical strategies were put forward to promote the new countryside construction in the local area, and provide references for the new countryside construction in other regions.
文摘[Objectives]This study was conducted to explore the mechanism of Ganlu Yin in the treatment of COVID-19 based on network pharmacology and molecular docking technology. [Methods] TCMSP, ETCM and TCMID databases were used to dig out the chemical components in the compound ingredients of Ganlu Yin. Swiss ADME platform was used for ADME screening and Swiss Target Prediction server was used to predict potential targets of chemical components. The COVID-19 related genes were obtained from GeneCards database and Drugbank website, and the intersection targets between Ganlu Yin and COVID-19 were obtained by Venn analysis. The targets were imported into String analysis platform to construct a protein interaction network, and Cytoscape 3.9.0 software was used for visualization processing. GO enrichment and KEGG pathway analysis were performed on the intersection targets through David database. The core components, clinical antiviral drugs, core targets and novel Coronavirus(SARS-COV-2) hydrolase 3 CLpro(Mpro), omicron B.1.1.529 spike protein and angiotensin converting enzyme 2(ACE2) were tested by molecular docking. [Results] A total of 213 effective chemical components and 1 023 drug targets in Ganlu Yin were screened out, including 4 620 COVID-19 related genes and 328 intersection targets. The core components mainly included β-sitosterol, quercetin, and kaherol. The key core targets involved GAPDH, AKT1, TNF, ALB, and EGFR, etc. A total of 241 items were screened by GO functional enrichment, including inflammatory response, protein phosphorylation, positive regulation of gene expression and other biological processes. A total of 85 KEGG pathways were screened out, which were mainly enriched in influenza A, HIF-1 signaling pathway, hepatitis C and other pathways. The results of molecular docking showed that the core chemical components in Ganlu Yin had good binding ability with core targets and disease targets ACE2, 3 CLpro and spike protein, especially kahenol with ACE2, β-sitosterol with 3 CLpro, and quercetin with spike protein. [Conclusions] Prescription Ganlu Yin has the characteristics of multi-component and multi-target action, and can treat COVID-19 through multiple signaling pathways.
文摘Objective:To observe the curative effect of Modified Ganlu Xiaodu Dan and Kuhuang San combined with interferon on the treatment of HFMD of damp-heat accumulation toxin syndrome and its effects on liver function, myocardial enzymes, inflammatory factors and immunoglobulin.Merhods:From June 2018 to November 2018, 74 children with damp-heat accumulation toxin syndrome who were diagnosed with hand, foot and mouth disease have been randomly divided into the observation group (38 cases) and the control group (36 cases).The control group is given conventional Western medicine symptomatic treatment, and sprayed on the oral cavity with IFN- atomized inhalation or IFN- 2b spray. On the basis of the control group, the observation group is given Modified Ganlu Xiaodu Dan, and Kuhuangsan for washing body. 7 d is a course of treatment. The recurrence time of the two groups, the time of rash regression and the efficacy of the children have been observed. The changes of liver function, myocardial zymogram, inflammatory factors and immunoglobulin are compared before and after treatment.Results: The efficacy of the observation group was 92.1% higher than that of the control group (83.3%), and the difference was statistically significant. The time required for the body temperature of the observation group (2.64±0.57) d was lower than that of the control group (3.12±0.62) d, and the time required for the rash to resolve was (4.18±0.69) d, which was lower than that of the control group (5.25±0.73) d. Statistically significant. The levels of ALT, AST, CK-MB, IL-4 and IL-6 in the observation group were lower than those in the control group after treatment, the difference was statistically significant. The serum IgA and IgM levels in the observation group were higher than those in the control group after treatment. Conclusion: Modified Ganlu Xiaodu Dan and Kuhuang San combined with interferon for the treatment of HFMD of damp-heat accumulation toxin syndrome have a sound curative effect and it can improve liver function, protect cardiomyocytes, reduce inflammatory factors, and regulate immune function for children.
基金Major national science and technology projects-prevention and treatment of major infectious diseases such as AIDS and viral hepatitis(No.2018ZX10303502)National Nature Fund Youth Fund(No.81804063)+2 种基金China postdoctoral Science Foundation Program(No.2019M662500)China postdoctoral Science Foundation Program(No.2019M662500)Key Scientific Research Project of National Clinical Research Base of traditional Chinese Medicine of Henan Provincial Administration of traditional Chinese Medicine(No.2018JDZX1100)
文摘Objective: To analyze the potential active components and molecular mechanism of Ganlu Xiaodu MicroPill in the treatment of COVID-19 by network pharmacology and molecular docking. Methods: the active chemical constituents and action targets of Ganlu Xiaodu MicroPill were searched by TCMSP and BATMAN-TCM platform, and the key targets were matched with the COVID-19 targets searched by CTD database. The core targets were selected and analyzed by GO and KEGG enrichment analysis by R software to predict the action mechanism of traditional Chinese medicine compound. The key component sets were docked with 3CLpro and ACE2. Results: 138 active components and 53 key targets were obtained. There are 1298 items in GO biological process, 59 items in cell composition, 62 items in molecular function, and 120 items enriched in KEGG pathway. Results: molecular docking showed that the affinity of 20 chemical components such as quercetin, luteolin and kaempferol with ACE2 and 3CLpro was higher than that of ribavirin and chloroquine. Conclusion: Ganlu Xiaodu MicroPill may regulate and interfere with SARS-CoV-2 infection through antivirus, inhibition of inflammatory factors and regulation of immunity, which fully reflects the therapeutic advantages of multi-components, multi-targets and multi-pathways of traditional Chinese medicine.
文摘目的:运用网络药理学及分子对接的方法,深入探索蒙药五味甘露治疗皮肤病的关键成分和作用靶点,从而揭示其作用机制。方法:依托中药系统药理学数据库与分析平台(TCMSP)筛选五味甘露组方药材的主要化学成分与作用靶点,借助GeneCards数据库收集皮肤病相关关键靶点,并用Cytoscape软件建立成分-靶点作用网络,刻画出关键成分和靶点。利用String数据库对关键靶点进行PPI网络/构建及拓扑分析,得到五味甘露治疗皮肤病的主要靶点,进而作GO富集和KEGG通路富集分析。Discovery Studio 2022软件CDOCKER模块进行分子对接,表征五味甘露治疗皮肤病的生物学过程和信号通路。结果:筛选得到五味甘露1638个化学成分作用的265个皮肤病相关疾病靶点。通过成分-靶点网络构建发现五味甘露主要依靠山柰酚、亚油酸、油酸、槲皮苷等10个成分作用于IL-6、PPARG、TP53、TLR4、VEGFA等50个主要靶点发挥治疗皮肤病的作用。上述靶点主要涉及DNA转录、氧化还原酶活性、炎症反应、细胞增殖及凋亡调控、蛋白结合等生物过程。KEGG信号通路分析显示,五味甘露治疗皮肤病主要涉及C型凝集素受体信号通路、Toll样受体等信号通路。分子对接结果显示五味甘露山柰酚、亚油酸、油酸、槲皮苷等活性成分与皮肤病作用于皮肤病IL-6、PPARG、TP53、TLR4、VEGFA等因子具有良好的结合活性。结论:五味甘露通过多个重要成分作用于皮肤病关键靶点,并通过调控白细胞迁移、伤口愈合、细胞增殖及凋亡调控等多个生物过程发挥作用,本研究为今后皮肤病的研究和治疗提供了机制基础。
