In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and t...In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and then upregulation.However,the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release.In this study,we further validated the effect of GA3 and hydrogen cyanamide(HC)on bud break of‘Red Globe’grape,confirmed inhibition and promotion effect,respectively.Restricted GA biosynthetic genes'expression and enhanced GA catabolic gene's expression were observed in the early stage after HC treatment,while opposite expression trend showed in the late stage.VvSVP1,a MADS-box transcription factor gene,was downregulated in the late stage,which might play an important role in regulating GA metabolism genes'expression.It was shown that,VvSVP1 could bind to the promoter regions of GA biosynthetic gene VvGA20ox6 and catabolic gene VvGA2ox3,negatively and positively regulated the corresponding genes'expression,respectively;the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed VvSVP1,while the ratio of GAs related to GA2ox were significantly increased.Taken together,VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC.Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.展开更多
Objectives:Triple-negative breast cancer(TNBC)presents a major treatment challenge due to its aggressive behavior.The dysfunction of the Golgi apparatus(GA)contributes to the development of various cancers.This study ...Objectives:Triple-negative breast cancer(TNBC)presents a major treatment challenge due to its aggressive behavior.The dysfunction of the Golgi apparatus(GA)contributes to the development of various cancers.This study aimed to utilize GA-related genes(GARGs)to forecast the prognosis and immune profile of TNBC.Methods:The data were downloaded from The Cancer Genome Atlas(TCGA)database,including 175 TNBC and 99 healthy samples.The differentially expressed GARGs(DEGARGs)were analyzed using the TCGA biolinks package.The patients with TNBC were classified into two clusters utilizing the ConsensusClusterPlus package according to prognosis-related DEGARGs,followed by comparing the differences in prognosis and immune infiltration between the two clusters.Next,LASSO and stepwise Cox regression were applied to establish a GARGs signature to forecast the TNBC prognosis.The association of the GARGs signature with immune infiltrates and drug sensitivity was further explored.Results:In total,430 DEGARGs were identified between TNBC and healthy samples,among which 20 were related to TNBC prognosis.Two GARG-related molecular clusters associated with different survival times and immune heterogeneity were identified.A risk model for TNBC was established based on six GARGs,and the high-risk(HR)group exhibited a poor prognosis.The HR group demonstrated a distinctly high M2 macrophage infiltration and low M1 macrophage infiltration,which contributed to an immunosuppressive tumor microenvironment and thus led to poor prognosis of the HR group.Immune dysfunction scores and programmed cell death ligand 1(PD-L1)expression were substantially elevated in the HR group.The HR group showed increased sensitivity to anticancer drugs,such as cisplatin.Conclusion:Our findings suggest that GARGs are involved in the pathogenesis of TNBC and provide new insights into prognostic prediction.The identified clusters and GARGs signatures have the potential to guide individualized therapy.展开更多
Due to the weak interlayer interactions,the binary Ⅲ-Ⅵ chalcogenides Ga Se can exist in several distinct polymorphs.Among them,the so-called β-and ε-phases simultaneously exhibit favorable total energies and moder...Due to the weak interlayer interactions,the binary Ⅲ-Ⅵ chalcogenides Ga Se can exist in several distinct polymorphs.Among them,the so-called β-and ε-phases simultaneously exhibit favorable total energies and moderate band gaps,which offer a good platform to explore their thermoelectric properties.Here,we demonstrate by first-principles calculations that the two systems have very similar band structures and phonon dispersions,despite different stacking sequences between adjacent layers.Interestingly,the lattice thermal conductivity of ε-GaSe is obviously lower than that of β-GaSe,which is inherently tied to stronger lattice anharmonicity caused by bonding heterogeneity.Besides,both systems exhibit higher p-type power factors due to doubly degenerate bands with weaker dispersions around the valence band maximum.As a consequence,a significantly enhanced p-type figure-of-merit of 2.1 can be realized at 700 K along the out-of-plane direction of theε-phase.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32172514).
文摘In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and then upregulation.However,the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release.In this study,we further validated the effect of GA3 and hydrogen cyanamide(HC)on bud break of‘Red Globe’grape,confirmed inhibition and promotion effect,respectively.Restricted GA biosynthetic genes'expression and enhanced GA catabolic gene's expression were observed in the early stage after HC treatment,while opposite expression trend showed in the late stage.VvSVP1,a MADS-box transcription factor gene,was downregulated in the late stage,which might play an important role in regulating GA metabolism genes'expression.It was shown that,VvSVP1 could bind to the promoter regions of GA biosynthetic gene VvGA20ox6 and catabolic gene VvGA2ox3,negatively and positively regulated the corresponding genes'expression,respectively;the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed VvSVP1,while the ratio of GAs related to GA2ox were significantly increased.Taken together,VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC.Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.
文摘Objectives:Triple-negative breast cancer(TNBC)presents a major treatment challenge due to its aggressive behavior.The dysfunction of the Golgi apparatus(GA)contributes to the development of various cancers.This study aimed to utilize GA-related genes(GARGs)to forecast the prognosis and immune profile of TNBC.Methods:The data were downloaded from The Cancer Genome Atlas(TCGA)database,including 175 TNBC and 99 healthy samples.The differentially expressed GARGs(DEGARGs)were analyzed using the TCGA biolinks package.The patients with TNBC were classified into two clusters utilizing the ConsensusClusterPlus package according to prognosis-related DEGARGs,followed by comparing the differences in prognosis and immune infiltration between the two clusters.Next,LASSO and stepwise Cox regression were applied to establish a GARGs signature to forecast the TNBC prognosis.The association of the GARGs signature with immune infiltrates and drug sensitivity was further explored.Results:In total,430 DEGARGs were identified between TNBC and healthy samples,among which 20 were related to TNBC prognosis.Two GARG-related molecular clusters associated with different survival times and immune heterogeneity were identified.A risk model for TNBC was established based on six GARGs,and the high-risk(HR)group exhibited a poor prognosis.The HR group demonstrated a distinctly high M2 macrophage infiltration and low M1 macrophage infiltration,which contributed to an immunosuppressive tumor microenvironment and thus led to poor prognosis of the HR group.Immune dysfunction scores and programmed cell death ligand 1(PD-L1)expression were substantially elevated in the HR group.The HR group showed increased sensitivity to anticancer drugs,such as cisplatin.Conclusion:Our findings suggest that GARGs are involved in the pathogenesis of TNBC and provide new insights into prognostic prediction.The identified clusters and GARGs signatures have the potential to guide individualized therapy.
基金supported by the National Natural Science Foundation of China(Grant Nos.62074114 and 12474019)。
文摘Due to the weak interlayer interactions,the binary Ⅲ-Ⅵ chalcogenides Ga Se can exist in several distinct polymorphs.Among them,the so-called β-and ε-phases simultaneously exhibit favorable total energies and moderate band gaps,which offer a good platform to explore their thermoelectric properties.Here,we demonstrate by first-principles calculations that the two systems have very similar band structures and phonon dispersions,despite different stacking sequences between adjacent layers.Interestingly,the lattice thermal conductivity of ε-GaSe is obviously lower than that of β-GaSe,which is inherently tied to stronger lattice anharmonicity caused by bonding heterogeneity.Besides,both systems exhibit higher p-type power factors due to doubly degenerate bands with weaker dispersions around the valence band maximum.As a consequence,a significantly enhanced p-type figure-of-merit of 2.1 can be realized at 700 K along the out-of-plane direction of theε-phase.
