Forty four adult male rats weighing about 250 g were divided into 3 groups: Group 1 received GTW at a does of 10 mg/kg/d, Group 2, 20mg/kg/d, and Group 3 was givea 1% carboxymethy! cellulose as control. The rats were ...Forty four adult male rats weighing about 250 g were divided into 3 groups: Group 1 received GTW at a does of 10 mg/kg/d, Group 2, 20mg/kg/d, and Group 3 was givea 1% carboxymethy! cellulose as control. The rats were sacrificed on展开更多
SD Twenty adult male rats were equally divided into a contro1 and an experimcntal group.GTW,10 mg/kg per day,was given to the experimental rats through gastric gavage 6 days a week for 8 weeks.To the control rats,the ...SD Twenty adult male rats were equally divided into a contro1 and an experimcntal group.GTW,10 mg/kg per day,was given to the experimental rats through gastric gavage 6 days a week for 8 weeks.To the control rats,the same amount of vehicle was given The animals were sacrificed when they became infertile and the fol1owing parameters of the testis and epididymis were examined:l)DNA(Feulgen′s method),2)RNA(Brachet′s tech-uique),3)LDH-X(Lojda′s method),4)ATPase(Wachstein′s procedure),5)Succinate dehydrc-genase(SDH,Pearson's method),6)Non-specific esterase(NSE,Lojda's technique)and 7)PAS reaction.Routine examination of the epididymal spermatozoa was also carried out.展开更多
To examine the effect of multi-glycoside of Tripterygium wilfordii Hook. f. （GTW） on proteinuria and acute glomerular immune lesion in experimental mesangial proliferative glomerulonephritis （MsPGN） induced by ant...To examine the effect of multi-glycoside of Tripterygium wilfordii Hook. f. （GTW） on proteinuria and acute glomerular immune lesion in experimental mesangial proliferative glomerulonephritis （MsPGN） induced by anti-Thyl. 1 monoelonal antibody （mAb） 1-22-3. The reversible model of MsPGN with anti-Thyl. 1 mAb 1-22-3 was established. After 7 days of oral treatment with GTW （ 100 mg/kg per day） and vehicle （distilled water, 5 ml/kg per day）, its effects on proteinuria, renal functions, mesangial morphological change, glomerular macrophage accumulation, and mRNA expressions of cytokines （PDGF-BB and MCP-1 ） were evaluated by light microscope （LM）, immunofluorescence （IF）, and reverse transcription polymerase chain reaction （RT-PCR）. It was found that GTW ameliorated proteinuria （on day 3 and day 7）, mesangial proliferation （total cell number, matrix expansion, a-smooth muscle actin expression, and collagen type Ⅰ expression） and macrophage accumulation （ED3^＋ ） in experimental MsPGN. In addition, GTW significantly suppressed the increased mRNA expressions for MCP-1 （67.6% to eontrol group, P 〈 0.01） together with the tendency to reduce the expression of PDGF （24.44% to control group） on day 7. It is concluded that GTW can not only decrease proteinuria, but also ameliorate acute mesangial alterations and glomerular activated macrophage accumulation probably by reduction of cytokines. These data indicate that GTW is an effective agent for early MsPGN.展开更多
本文报道用原位缺口翻译法,研究男用节育药醋酸棉酚和雷公藤总甙(GTW)对C3H-10 T 1/2小鼠成纤维细胞DNA的作用。结果表明,在较高浓度的醋酸棉酚或GTW(2～3μg/ml)作用4小时,细胞核中显示的银颗粒较多,如阳性对照组的N-甲基-N′-硝基-亚...本文报道用原位缺口翻译法,研究男用节育药醋酸棉酚和雷公藤总甙(GTW)对C3H-10 T 1/2小鼠成纤维细胞DNA的作用。结果表明,在较高浓度的醋酸棉酚或GTW(2～3μg/ml)作用4小时,细胞核中显示的银颗粒较多,如阳性对照组的N-甲基-N′-硝基-亚硝基胍(MNNG)组;在中等浓度的(0.5～1μg/ml)作用下,银颗粒显著地少得多;在较低浓度的醋酸棉酚或GTW作用下(0.1～0.3μg/ml),细胞核中呈现的银颗粒与阴性对照组相似。与原位缺口翻译实验相对应,集落形成试验表明,在高浓度醋酸棉酚或GTW作用下,细胞失去繁殖的能力;而在中等或较低浓度的药物作用下,仍有较高的集落形成率。这些观察表明,高浓度的醋酸棉酚或GTW对细胞DNA有严重的损伤作用,而在中等浓度的药物作用下,DNA受到某种程度的损伤,但可修复;低浓度的醋酸棉酚或GTW对细胞DNA无明显的损伤作用,本文对这些结果的意义进行了简要的讨论。展开更多
文摘Forty four adult male rats weighing about 250 g were divided into 3 groups: Group 1 received GTW at a does of 10 mg/kg/d, Group 2, 20mg/kg/d, and Group 3 was givea 1% carboxymethy! cellulose as control. The rats were sacrificed on
文摘SD Twenty adult male rats were equally divided into a contro1 and an experimcntal group.GTW,10 mg/kg per day,was given to the experimental rats through gastric gavage 6 days a week for 8 weeks.To the control rats,the same amount of vehicle was given The animals were sacrificed when they became infertile and the fol1owing parameters of the testis and epididymis were examined:l)DNA(Feulgen′s method),2)RNA(Brachet′s tech-uique),3)LDH-X(Lojda′s method),4)ATPase(Wachstein′s procedure),5)Succinate dehydrc-genase(SDH,Pearson's method),6)Non-specific esterase(NSE,Lojda's technique)and 7)PAS reaction.Routine examination of the epididymal spermatozoa was also carried out.
文摘To examine the effect of multi-glycoside of Tripterygium wilfordii Hook. f. （GTW） on proteinuria and acute glomerular immune lesion in experimental mesangial proliferative glomerulonephritis （MsPGN） induced by anti-Thyl. 1 monoelonal antibody （mAb） 1-22-3. The reversible model of MsPGN with anti-Thyl. 1 mAb 1-22-3 was established. After 7 days of oral treatment with GTW （ 100 mg/kg per day） and vehicle （distilled water, 5 ml/kg per day）, its effects on proteinuria, renal functions, mesangial morphological change, glomerular macrophage accumulation, and mRNA expressions of cytokines （PDGF-BB and MCP-1 ） were evaluated by light microscope （LM）, immunofluorescence （IF）, and reverse transcription polymerase chain reaction （RT-PCR）. It was found that GTW ameliorated proteinuria （on day 3 and day 7）, mesangial proliferation （total cell number, matrix expansion, a-smooth muscle actin expression, and collagen type Ⅰ expression） and macrophage accumulation （ED3^＋ ） in experimental MsPGN. In addition, GTW significantly suppressed the increased mRNA expressions for MCP-1 （67.6% to eontrol group, P 〈 0.01） together with the tendency to reduce the expression of PDGF （24.44% to control group） on day 7. It is concluded that GTW can not only decrease proteinuria, but also ameliorate acute mesangial alterations and glomerular activated macrophage accumulation probably by reduction of cytokines. These data indicate that GTW is an effective agent for early MsPGN.
文摘本文报道用原位缺口翻译法,研究男用节育药醋酸棉酚和雷公藤总甙(GTW)对C3H-10 T 1/2小鼠成纤维细胞DNA的作用。结果表明,在较高浓度的醋酸棉酚或GTW(2～3μg/ml)作用4小时,细胞核中显示的银颗粒较多,如阳性对照组的N-甲基-N′-硝基-亚硝基胍(MNNG)组;在中等浓度的(0.5～1μg/ml)作用下,银颗粒显著地少得多;在较低浓度的醋酸棉酚或GTW作用下(0.1～0.3μg/ml),细胞核中呈现的银颗粒与阴性对照组相似。与原位缺口翻译实验相对应,集落形成试验表明,在高浓度醋酸棉酚或GTW作用下,细胞失去繁殖的能力;而在中等或较低浓度的药物作用下,仍有较高的集落形成率。这些观察表明,高浓度的醋酸棉酚或GTW对细胞DNA有严重的损伤作用,而在中等浓度的药物作用下,DNA受到某种程度的损伤,但可修复;低浓度的醋酸棉酚或GTW对细胞DNA无明显的损伤作用,本文对这些结果的意义进行了简要的讨论。
