The callipyge （CLPG） phenotype, exhibiting polar overdominance （POD）, is an inherited skeletal muscle hypertrophy described in sheep. The callipyge locus maps to the distal portion of ovine chromosome 18 within th...The callipyge （CLPG） phenotype, exhibiting polar overdominance （POD）, is an inherited skeletal muscle hypertrophy described in sheep. The callipyge locus maps to the distal portion of ovine chromosome 18 within the DLKI-GTL2 region and corresponds to human chromosome 14 and mouse chromosome 12. The POD phenomenon is confirmed to the homologous region of swine chromosome 7. In order to clone and investigate the expression of porcine GTL2 gene, DNA and RNA samples from 60-day-old F1 animals, generated with reciprocal crosses between Large White and Meishan breeds and their parents, were used. The authors showed that porcine GTL2 acted as a uoncoding RNA. cDNA samples exhibited maternal expression of the gene in the heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, and fat in pigs, and a unique tissue-specific expression different from that of humans and mice. These results indicated that the gene was conserved in the pig, human, mouse, and bovine. It will be of interest to further study the gene functions in muscle growth and fat deposition.展开更多
Epigenetic reprogramming of somatic cells into induced pluripotent stem cells （iPSCs） by overexpression of defined factors holds great promise for disease modeling and regen- erative medicine （Takahashi and Yamanak...Epigenetic reprogramming of somatic cells into induced pluripotent stem cells （iPSCs） by overexpression of defined factors holds great promise for disease modeling and regen- erative medicine （Takahashi and Yamanaka, 2006; Robinton and Daley, 2012）. However, the stochastic reprogramming process often results in variable pluripotency levels of iPSC lines as measured by their in vivo developmental potential, which poses a huge challenge to the applications of high quality iPSCs （Hanna et al., 2010）. The activation status of an imprinted Dlkl-Dio3 region has been identified as a molecular marker for pluripotency （Liu et al., 2010; Stadtfeld et al.,展开更多
基金supported by theNational Natural Science Foundation of China(30571331).
文摘The callipyge （CLPG） phenotype, exhibiting polar overdominance （POD）, is an inherited skeletal muscle hypertrophy described in sheep. The callipyge locus maps to the distal portion of ovine chromosome 18 within the DLKI-GTL2 region and corresponds to human chromosome 14 and mouse chromosome 12. The POD phenomenon is confirmed to the homologous region of swine chromosome 7. In order to clone and investigate the expression of porcine GTL2 gene, DNA and RNA samples from 60-day-old F1 animals, generated with reciprocal crosses between Large White and Meishan breeds and their parents, were used. The authors showed that porcine GTL2 acted as a uoncoding RNA. cDNA samples exhibited maternal expression of the gene in the heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, and fat in pigs, and a unique tissue-specific expression different from that of humans and mice. These results indicated that the gene was conserved in the pig, human, mouse, and bovine. It will be of interest to further study the gene functions in muscle growth and fat deposition.
基金supported by the grants from the "Strategic Priority Research Program" of the Chinese Academy of Sciences (No. XDA01020100 to Q.Z.)the China National Basic Research Program (No. 2012CBA01300 to Q.Z.)the National Science Foundation of China (No. 91319308 to Q.Z.,31201105 to L.L. and 31371516 to W.L.)
文摘Epigenetic reprogramming of somatic cells into induced pluripotent stem cells （iPSCs） by overexpression of defined factors holds great promise for disease modeling and regen- erative medicine （Takahashi and Yamanaka, 2006; Robinton and Daley, 2012）. However, the stochastic reprogramming process often results in variable pluripotency levels of iPSC lines as measured by their in vivo developmental potential, which poses a huge challenge to the applications of high quality iPSCs （Hanna et al., 2010）. The activation status of an imprinted Dlkl-Dio3 region has been identified as a molecular marker for pluripotency （Liu et al., 2010; Stadtfeld et al.,
