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糖基翻转酶gtcA基因缺失降低单增李斯特菌1/2a血清型菌株致病性的研究 被引量:5
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作者 郭骞 张钰 +3 位作者 方小伟 安妮 袁梅 方春 《中国畜牧兽医》 CAS 北大核心 2022年第6期2298-2306,共9页
【目的】构建糖基翻转酶gtcA基因缺失株,并阐明其对单增李斯特致病性的影响。【方法】利用同源重组技术构建gtcA基因缺失株;进一步分析亲本株EGDe-prfA*、缺失株ΔgtcA和回补株CΔgtcA的生长能力;通过Western blotting分析gtcA基因缺失... 【目的】构建糖基翻转酶gtcA基因缺失株,并阐明其对单增李斯特致病性的影响。【方法】利用同源重组技术构建gtcA基因缺失株;进一步分析亲本株EGDe-prfA*、缺失株ΔgtcA和回补株CΔgtcA的生长能力;通过Western blotting分析gtcA基因缺失对InlA和InlB在细菌表面锚定的影响;通过DF1细胞黏附侵袭试验、RAW264.7细胞吞噬增殖试验和鸡胚毒力试验评估gtcA基因缺失对单增李斯特菌细胞侵染能力及对鸡胚致病性的影响。【结果】生长曲线显示,gtcA基因缺失并不影响单增李斯特菌在BHI培养基中的生长能力。Western blotting结果显示,内化素InlB在ΔgtcA表面的锚定量极显著低于EGDe-prfA*和CΔgtcA(P<0.01),但其表面InlA的锚定量与EGDe-prfA*并无显著差异(P>0.05)。细胞黏附侵袭试验结果显示,ΔgtcA对成纤维细胞DF1的平均黏附率和侵袭率显著低于EGDe-prfA*和CΔgtcA(P<0.05)。吞噬试验结果显示,巨噬细胞RAW264.7对ΔgtcA的平均吞噬率显著低于EGDe-prfA*和CΔgtcA(P<0.05),但ΔgtcA与EGDe-prfA*、CΔgtcA在RAW264.7中的3 h内增殖倍数并无显著差异(P>0.05)。鸡胚毒力试验结果显示,ΔgtcA感染鸡胚肝脏和脾脏中的平均细菌载量分别为6.86×10^(3)和3.69×10^(2) CFU,极显著低于EGDe-prfA*和CΔgtcA感染鸡胚(P<0.01);同时,ΔgtcA感染鸡胚存活率及存活时间均高于EGDe-prfA*和CΔgtcA感染鸡胚。【结论】糖基转移酶gtcA基因缺失不影响单增李斯特菌1/2a血清型菌株EGDe-prfA*的生长能力,但能显著降低该菌表面InlB的锚定丰度,减弱该菌的细胞侵染能力及对鸡胚的致病性。本研究结果为深入探索糖基翻转酶基因gtcA介导单增李斯特菌致病机制提供参考。 展开更多
关键词 单增李斯特菌 糖基翻转酶 gtca基因 缺失 致病性
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Influence of magnetically constricted arc traverse speed (MCATS) on tensile properties and microstructural characteristics of welded Inconel 718 alloy sheets 被引量:1
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作者 Tushar Sonar Visvalingam Balasubramanian +2 位作者 Sudersanan Malarvizhi Thiruvenkatam Venkateswaran Dhenuvakonda Sivakumar 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2021年第4期1395-1413,共19页
The magnetically constricted arc technique was implemented to mitigate the heat input related metallurgical problems in Gas Tungsten Arc Welding(GTAW)of Inconel 718 alloy particularly Nb segregation and subsequent lav... The magnetically constricted arc technique was implemented to mitigate the heat input related metallurgical problems in Gas Tungsten Arc Welding(GTAW)of Inconel 718 alloy particularly Nb segregation and subsequent laves phase evolution in fusion zone.This paper reports the direct effect of magnetically constricted arc traverse speed(MCATS)on bead profile,tensile properties and microstructural evolution of Inconel 718 alloy sheets joined by Gas Tungsten Constricted Arc Welding(GTCAW)process.The mechanism amenable for the microstructural modification and corresponding influence on the tensile properties of joints is investigated both in qualitative and quantitative manner related to the mechanics of arc constriction and pulsing.It is correlated to the solidification conditions during welding.The relationship between MCATS and Arc Constriction Current(ACC)was derived.Its interaction effect on the magnetic arc constriction and joint performance was analysed.Results showed that the joints fabricated using CATS of 70 mm/min exhibited superior tensile properties(98.39% of base metal strength with 31.50% elongation).It is attributed to the grain refinement in fusion zone microstructure leading to the evolution of finer,discrete laves phase in interdendritic areas. 展开更多
关键词 gtca welding Inconel 718 alloy Magnetically constricted arc traverse speed Tensile properties Microstructure Laves phase
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