Although Yarrowia lipolytica is an important host strain, there have so far been few studies on the production of glutathione by the strain. We therefore performed a study to obtain an improved strain of Y. lipolytica...Although Yarrowia lipolytica is an important host strain, there have so far been few studies on the production of glutathione by the strain. We therefore performed a study to obtain an improved strain of Y. lipolytica ATCC20688, which could produce a high yield of glutathione. First, the capability of glutathione production in the ATCC20688 strain was estimated. In comparison with other yeasts, the yield of this strain was higher than those in Pichia strains. Furthermore, this strain could produce glutathione by assimilating sodium oleate. We next performed mutation and gene cloning to improve the yield. After the yield of glutathione was improved in the isolated methylglyoxal-resistant mutant (MGR3), the glutathione synthetase gene was cloned into the MGR3 strain. By using this recombinant strain, we could reach the maximum yield and intracellular content of glutathione of 54 mg/L-medium and 30 mg/g-dry cell weight, respectively.展开更多
The controllable growth of metal nanoparticles on nanomaterials is becoming an effective strategy for developing nanocomposites with designated performance.Herein,a simple and mild strategy for the in situ growth of P...The controllable growth of metal nanoparticles on nanomaterials is becoming an effective strategy for developing nanocomposites with designated performance.Herein,a simple and mild strategy for the in situ growth of Pt–Pd bimetallic nanoparticles on covalent organic frameworks(COFs)to regulate the nanozyme activity was designed for colorimetric detection of hydrogen peroxide(H2O2)and glutathione(GSH).The COFs not only offer sufficient loading sites for the uniform dispersion of Pt–Pd bimetallic nanoparticles,but also increase the adsorption of substrate to promote the catalytic reaction.With the bimetallic synergistic effect of Pt–Pd nanoparticles,the prepared multifunctional nanozyme(Pt–Pd/COFs nanozyme)simultaneously exhibited superior peroxidase(POD)-like activity and oxidase(OXD)-like activity.Using the multifunctional nanozyme,a colorimetric sensing system was constructed for sensitive detection of H2O2 and GSH,with the wide linear ranges of 5–1000µmol/L and 1–40µmol/L,and the detection limits were 1.14μmol/L and 0.43μmol/L,respectively.It was successfully used for the detection of real samples in environmental water and serum,providing a simple method for disease diagnosis and environmental monitoring.展开更多
文摘Although Yarrowia lipolytica is an important host strain, there have so far been few studies on the production of glutathione by the strain. We therefore performed a study to obtain an improved strain of Y. lipolytica ATCC20688, which could produce a high yield of glutathione. First, the capability of glutathione production in the ATCC20688 strain was estimated. In comparison with other yeasts, the yield of this strain was higher than those in Pichia strains. Furthermore, this strain could produce glutathione by assimilating sodium oleate. We next performed mutation and gene cloning to improve the yield. After the yield of glutathione was improved in the isolated methylglyoxal-resistant mutant (MGR3), the glutathione synthetase gene was cloned into the MGR3 strain. By using this recombinant strain, we could reach the maximum yield and intracellular content of glutathione of 54 mg/L-medium and 30 mg/g-dry cell weight, respectively.
基金financially supported by the National Natural Science Foundation of China(22076041,22076042)the Key Research and Development Program of Hubei Province,China(2023BAB134).
文摘The controllable growth of metal nanoparticles on nanomaterials is becoming an effective strategy for developing nanocomposites with designated performance.Herein,a simple and mild strategy for the in situ growth of Pt–Pd bimetallic nanoparticles on covalent organic frameworks(COFs)to regulate the nanozyme activity was designed for colorimetric detection of hydrogen peroxide(H2O2)and glutathione(GSH).The COFs not only offer sufficient loading sites for the uniform dispersion of Pt–Pd bimetallic nanoparticles,but also increase the adsorption of substrate to promote the catalytic reaction.With the bimetallic synergistic effect of Pt–Pd nanoparticles,the prepared multifunctional nanozyme(Pt–Pd/COFs nanozyme)simultaneously exhibited superior peroxidase(POD)-like activity and oxidase(OXD)-like activity.Using the multifunctional nanozyme,a colorimetric sensing system was constructed for sensitive detection of H2O2 and GSH,with the wide linear ranges of 5–1000µmol/L and 1–40µmol/L,and the detection limits were 1.14μmol/L and 0.43μmol/L,respectively.It was successfully used for the detection of real samples in environmental water and serum,providing a simple method for disease diagnosis and environmental monitoring.
