The aim of the current study was to investigate the process of microgametogenesis and diagnostic of condition of the Pinus sylvestris male generative system in the tree stands exposed to the influence of Reftinskiy GR...The aim of the current study was to investigate the process of microgametogenesis and diagnostic of condition of the Pinus sylvestris male generative system in the tree stands exposed to the influence of Reftinskiy GRES power plant (RGpp) emission during ontogenesis in connection with the level of technogenic pollution. It was detected that in the impact area, smoke emissions from the RGpp in the south-west direction zones of technogenic pollution were formed. Those vary in qualitative composition of the snow pack pollutants and their quantitative characteristics. The content of water insoluble compounds in melt snow water and the compile suspended matter, decreases regularly at the longer distance from the source of air pollutants. The highest content of water soluble compounds in the melt snow water was indicated at the distances of 5, 8 and 14 km from the source of air pollutants, authentically lower in the nearest and farthest sampling points of the snow trials. Scots pine (Pinus sylvestris) pollen significative with high prognostic value in relation to the level of toxic load upon the eco-systems and the condition of male generative system were discovered. It was detected that the significative meanings of pollen with morphological abnormalities decreased, and the meanings of functional significatives increased at the longer distance from the source of air pollutants. Pollen fertility, pollen tube length and reserve substances content significative is negatively related to the presence of micro and macro elements in the snow water;those identify the toxic load level on the ecosystem probably to the highest degree. Pine male gametophyte elimination, while forming in the impact zone of the GRES power plant, takes place at the stage of pollen grain germination as well as pollen tubes’ growth stage.展开更多
文章介绍GRE、IPSec和无线网络的相关技术理论,对“一校多区”的无线校园网络提出了一个基于GRE over IPSec VPN实现AP上线统一管理方案,并通过HCL模拟软件进行实验仿真。实验结果表明,该方案可以跨区域安全稳定传输私网数据,实现AP上...文章介绍GRE、IPSec和无线网络的相关技术理论,对“一校多区”的无线校园网络提出了一个基于GRE over IPSec VPN实现AP上线统一管理方案,并通过HCL模拟软件进行实验仿真。实验结果表明,该方案可以跨区域安全稳定传输私网数据,实现AP上线统一管理,无线业务运行正常。方案在一定程度上避免设备重复投资,节约了成本,提高了管理效率。实验涉及OSPF、GRE over IPSec VPN和无线相关配置等,通过具体步骤和配置助力校园网无线网络管理,为实际网络的建设提供有效参考和依据。展开更多
It is a trend of virtual private networks (VPNs) to be used for information exchange between enterprises, branches of enterprises and enterprises and their employees instead of traditional dial networks and leased lin...It is a trend of virtual private networks (VPNs) to be used for information exchange between enterprises, branches of enterprises and enterprises and their employees instead of traditional dial networks and leased lines. The tunneling technique is the key technique to implement VPN. In this paper, with the VPN implementation requirements in mind, we perform a comparative research on the existing tunneling protocols including GRE, L2TP, IPSec and IP/IP. We also propose an integrated scheme of tunneling mechanism that supports VPN under the current condition.展开更多
RNA沉默技术作为探索基因功能的实验手段应用于多种生物.以编码酿酒酵母NADPH依赖型醛糖还原酶的GRE3基因为对象,检测酿酒酵母双链RNA介导的基因沉默效应.以pESC-LEU为骨架,构建重组质粒psiLENT-GRE3并用于转化酿酒酵母YPH499.用RT-PCR...RNA沉默技术作为探索基因功能的实验手段应用于多种生物.以编码酿酒酵母NADPH依赖型醛糖还原酶的GRE3基因为对象,检测酿酒酵母双链RNA介导的基因沉默效应.以pESC-LEU为骨架,构建重组质粒psiLENT-GRE3并用于转化酿酒酵母YPH499.用RT-PCR检测到诱导1 kb RNA双螺旋和136 bp loop结构引起的GRE3基因表达下调.结果表明,双链RNA介导的基因沉默技术,能够用作降低酿酒酵母某一特定基因表达水平的工具.并有助于理解芽殖酵母的RNA干扰现象.展开更多
文摘The aim of the current study was to investigate the process of microgametogenesis and diagnostic of condition of the Pinus sylvestris male generative system in the tree stands exposed to the influence of Reftinskiy GRES power plant (RGpp) emission during ontogenesis in connection with the level of technogenic pollution. It was detected that in the impact area, smoke emissions from the RGpp in the south-west direction zones of technogenic pollution were formed. Those vary in qualitative composition of the snow pack pollutants and their quantitative characteristics. The content of water insoluble compounds in melt snow water and the compile suspended matter, decreases regularly at the longer distance from the source of air pollutants. The highest content of water soluble compounds in the melt snow water was indicated at the distances of 5, 8 and 14 km from the source of air pollutants, authentically lower in the nearest and farthest sampling points of the snow trials. Scots pine (Pinus sylvestris) pollen significative with high prognostic value in relation to the level of toxic load upon the eco-systems and the condition of male generative system were discovered. It was detected that the significative meanings of pollen with morphological abnormalities decreased, and the meanings of functional significatives increased at the longer distance from the source of air pollutants. Pollen fertility, pollen tube length and reserve substances content significative is negatively related to the presence of micro and macro elements in the snow water;those identify the toxic load level on the ecosystem probably to the highest degree. Pine male gametophyte elimination, while forming in the impact zone of the GRES power plant, takes place at the stage of pollen grain germination as well as pollen tubes’ growth stage.
文摘文章介绍GRE、IPSec和无线网络的相关技术理论,对“一校多区”的无线校园网络提出了一个基于GRE over IPSec VPN实现AP上线统一管理方案,并通过HCL模拟软件进行实验仿真。实验结果表明,该方案可以跨区域安全稳定传输私网数据,实现AP上线统一管理,无线业务运行正常。方案在一定程度上避免设备重复投资,节约了成本,提高了管理效率。实验涉及OSPF、GRE over IPSec VPN和无线相关配置等,通过具体步骤和配置助力校园网无线网络管理,为实际网络的建设提供有效参考和依据。
文摘It is a trend of virtual private networks (VPNs) to be used for information exchange between enterprises, branches of enterprises and enterprises and their employees instead of traditional dial networks and leased lines. The tunneling technique is the key technique to implement VPN. In this paper, with the VPN implementation requirements in mind, we perform a comparative research on the existing tunneling protocols including GRE, L2TP, IPSec and IP/IP. We also propose an integrated scheme of tunneling mechanism that supports VPN under the current condition.
文摘RNA沉默技术作为探索基因功能的实验手段应用于多种生物.以编码酿酒酵母NADPH依赖型醛糖还原酶的GRE3基因为对象,检测酿酒酵母双链RNA介导的基因沉默效应.以pESC-LEU为骨架,构建重组质粒psiLENT-GRE3并用于转化酿酒酵母YPH499.用RT-PCR检测到诱导1 kb RNA双螺旋和136 bp loop结构引起的GRE3基因表达下调.结果表明,双链RNA介导的基因沉默技术,能够用作降低酿酒酵母某一特定基因表达水平的工具.并有助于理解芽殖酵母的RNA干扰现象.
