Cyprus has an extensive record in grape production and winemaking.Grapevine is essential for the economic and environmental sustainability of the agricultural sector,as it is in other Mediterranean regions.Intensive a...Cyprus has an extensive record in grape production and winemaking.Grapevine is essential for the economic and environmental sustainability of the agricultural sector,as it is in other Mediterranean regions.Intensive agriculture can overuse and exhaust natural resources,including soil and water.The current study evaluated how conservation strategies,including no tillage and semi-tillage(as a variation of strip tillage),affected grapevine growth and grape quality when compared to conventional tillage application.Two cultivars were used:Chardonnay and Maratheftiko(indigenous).Soil pH decreased,and EC increased after tillage applications,in both vineyards.Tillage lowered soil N levels through mineralization,but the vineyard with Maratheftiko cultivar had higher soil N and K levels than the vineyard with Chardonnay cultivar.No tillage reduced yield in Chardonnay;however,semi-tillage enhanced yield in Maratheftiko.There were no major changes in plant physiology,even though Maratheftiko had less variation in stomatal conductance values under the various tillage practices than Chardonnay.Tillage enhanced N and K content in Chardonnay vines during flowering,and increased N in Maratheftiko.Total phenols and antioxidant status of leaves varied,with tillage stimulating them,especially at the harvest stage.Furthermore,tillage raised grape juice total soluble solids,pH,and total phenols in both cultivars,while anthocyanins and tannins content were increased in Maratheftiko under no tillage.The results of this study may aid in the development of cultivation strategies to enable viticulture to address various environmental challenges due to climate change.展开更多
The embryo rescue technique plays an essential role in developing new seedless grape varieties.To enhance the efficiency of seedless grape embryo rescue breeding,this study evaluated 22 hybrid combinations and systema...The embryo rescue technique plays an essential role in developing new seedless grape varieties.To enhance the efficiency of seedless grape embryo rescue breeding,this study evaluated 22 hybrid combinations and systematically investigated the effects of parental genotypes and plant hormones on embryo development and germination.Additionally,an in-depth analysis was conducted on the conversion of abnormal plantlets.Results indicate that‘Ruby Seedless’,‘Delight’,‘Huozhouheiyu’,‘Zitian Seedless’,and‘Zhengyan Seedless’are suitable as maternal parents,whereas‘Zitian Seedless’,‘Shennongxiangfeng’,‘Hongqitezao’,and‘Guibao’perform optimally as paternal parents.Among these,the crosses‘Ruby Seedless×Shennongxiangfeng’and‘Ruby Seedless×Zitian Seedless’exhibited the highest embryo rescue efficiency,with embryo development rates of 55.05 and 59.76%,yielding 1,348 and 2,235 viable plantlets,respectively.When 1.0 mg L^(–1) zeatin (ZT) was added to the MM3 medium supplemented with 0.2 mg L^(–1) indole-3-acetic acid (IAA),the embryo development rate of‘Ruby Seedless×Zitian Seedless’increased by 64.73%.In the WPM germination medium,supplementation with 0.2 mg L^(–1) ZT and 0.2 mg L^(–1) IAA resulted in the highest germination rate of 85.71%for the hybrid combination‘Huozhouheiyu×Shine Muscat’.Furthermore,3,365 abnormal plantlets were rescued via direct transformation and hypocotyl-induced adventitious bud regeneration,among which 1,234 were transformed into normal plantlets.Following hybridization,a total of 4,287 plants were successfully acclimatized and transplanted.This study provides theoretical insights to improve the efficiency of embryo rescue breeding in seedless grapes and offers valuable genetic resources for future breeding programs.展开更多
GRAPES(global and regional assimilation and prediction system)是由中国气象科学研究院灾害天气国家重点实验室自主研究开发的中国新一代数值天气预报系统,其目标是科研/业务通用.为了实现这一目标,结合高性能计算机的体系结构设计...GRAPES(global and regional assimilation and prediction system)是由中国气象科学研究院灾害天气国家重点实验室自主研究开发的中国新一代数值天气预报系统,其目标是科研/业务通用.为了实现这一目标,结合高性能计算机的体系结构设计并实现模式的并行计算是必不可少的.作为核心开发技术之一,GRAPES系统设计并实现了模式的并行计算方案,包括中尺度有限区模式的并行计算和全球模式并行计算.GRAPES模式并行计算版本在IBM-Cluster1600上的测试表明,GRAPES模式的并行计算程序正确、稳定、有效,为其业务化之路奠定了基础,同时也为系统未来的可持续开发、优化创造了条件.展开更多
基金financed by PRIMA(MiDiVine project),the European Union(ERA-NET Cofound FACCE SURPLUS Call of Horizon 2020-FACCE JPI,“VitiSmart”project)a programme supported by the European Union with co-funding by the Funding Agencies RIF-Cyprus。
文摘Cyprus has an extensive record in grape production and winemaking.Grapevine is essential for the economic and environmental sustainability of the agricultural sector,as it is in other Mediterranean regions.Intensive agriculture can overuse and exhaust natural resources,including soil and water.The current study evaluated how conservation strategies,including no tillage and semi-tillage(as a variation of strip tillage),affected grapevine growth and grape quality when compared to conventional tillage application.Two cultivars were used:Chardonnay and Maratheftiko(indigenous).Soil pH decreased,and EC increased after tillage applications,in both vineyards.Tillage lowered soil N levels through mineralization,but the vineyard with Maratheftiko cultivar had higher soil N and K levels than the vineyard with Chardonnay cultivar.No tillage reduced yield in Chardonnay;however,semi-tillage enhanced yield in Maratheftiko.There were no major changes in plant physiology,even though Maratheftiko had less variation in stomatal conductance values under the various tillage practices than Chardonnay.Tillage enhanced N and K content in Chardonnay vines during flowering,and increased N in Maratheftiko.Total phenols and antioxidant status of leaves varied,with tillage stimulating them,especially at the harvest stage.Furthermore,tillage raised grape juice total soluble solids,pH,and total phenols in both cultivars,while anthocyanins and tannins content were increased in Maratheftiko under no tillage.The results of this study may aid in the development of cultivation strategies to enable viticulture to address various environmental challenges due to climate change.
基金supported by the China Agriculture Research System of MOF and MARA (CARS-29-yc-3)。
文摘The embryo rescue technique plays an essential role in developing new seedless grape varieties.To enhance the efficiency of seedless grape embryo rescue breeding,this study evaluated 22 hybrid combinations and systematically investigated the effects of parental genotypes and plant hormones on embryo development and germination.Additionally,an in-depth analysis was conducted on the conversion of abnormal plantlets.Results indicate that‘Ruby Seedless’,‘Delight’,‘Huozhouheiyu’,‘Zitian Seedless’,and‘Zhengyan Seedless’are suitable as maternal parents,whereas‘Zitian Seedless’,‘Shennongxiangfeng’,‘Hongqitezao’,and‘Guibao’perform optimally as paternal parents.Among these,the crosses‘Ruby Seedless×Shennongxiangfeng’and‘Ruby Seedless×Zitian Seedless’exhibited the highest embryo rescue efficiency,with embryo development rates of 55.05 and 59.76%,yielding 1,348 and 2,235 viable plantlets,respectively.When 1.0 mg L^(–1) zeatin (ZT) was added to the MM3 medium supplemented with 0.2 mg L^(–1) indole-3-acetic acid (IAA),the embryo development rate of‘Ruby Seedless×Zitian Seedless’increased by 64.73%.In the WPM germination medium,supplementation with 0.2 mg L^(–1) ZT and 0.2 mg L^(–1) IAA resulted in the highest germination rate of 85.71%for the hybrid combination‘Huozhouheiyu×Shine Muscat’.Furthermore,3,365 abnormal plantlets were rescued via direct transformation and hypocotyl-induced adventitious bud regeneration,among which 1,234 were transformed into normal plantlets.Following hybridization,a total of 4,287 plants were successfully acclimatized and transplanted.This study provides theoretical insights to improve the efficiency of embryo rescue breeding in seedless grapes and offers valuable genetic resources for future breeding programs.
文摘GRAPES(global and regional assimilation and prediction system)是由中国气象科学研究院灾害天气国家重点实验室自主研究开发的中国新一代数值天气预报系统,其目标是科研/业务通用.为了实现这一目标,结合高性能计算机的体系结构设计并实现模式的并行计算是必不可少的.作为核心开发技术之一,GRAPES系统设计并实现了模式的并行计算方案,包括中尺度有限区模式的并行计算和全球模式并行计算.GRAPES模式并行计算版本在IBM-Cluster1600上的测试表明,GRAPES模式的并行计算程序正确、稳定、有效,为其业务化之路奠定了基础,同时也为系统未来的可持续开发、优化创造了条件.
