Background:Acute thrombotic events play a major role in various cardio-vascular diseases.Therefore,direct thrombus imaging can be proved beneficial for early diagnosis and prompt therapy of thrombosis.Our study invest...Background:Acute thrombotic events play a major role in various cardio-vascular diseases.Therefore,direct thrombus imaging can be proved beneficial for early diagnosis and prompt therapy of thrombosis.Our study investigated targeted dual‐modality cyclic arginine‐glycine‐aspartic micro bubbles(cRGD‐MBs)for direct imaging of thrombi by fluorescence and ultrasound.Methods:cRGD‐MBs were prepared by mechanical vibration and chemical chelation methods.Results:Coulter counter analysis demonstrated that the cRGD‐MBs were well dispersed,with diameters ranging from 1 to 3μm.They emitted bright red fluorescence under an excitation wavelength of 660 nm.In vivo fluorescence and ultrasound imaging revealed that cRGD‐MBs accumulated at the site of thrombus in the carotid artery with significant fluorescence and ultrasonic signal.Conclusions:This study showed that novel microbubble cRGD‐MBs were successfully synthesized,and that these could potentially be used as contrast agents for immediate diagnosis of acute thrombus in vivo.展开更多
BACKGROUND Non-small cell lung cancer(NSCLC)is the most prevalent subtype of lung cancer,accounting for approximately 85%of all lung cancer cases and remaining a major cause of cancer-related mortality worldwide.Despi...BACKGROUND Non-small cell lung cancer(NSCLC)is the most prevalent subtype of lung cancer,accounting for approximately 85%of all lung cancer cases and remaining a major cause of cancer-related mortality worldwide.Despite advances in diagnostic and therapeutic approaches,the incidence and mortality rates of NSCLC continue to rise,especially in low-income and middle-income countries.AIM To investigate the expression of cancer stem cell(CSC)markers and their relationship with the prognosis and survival of patients with stage IIIA NSCLC.METHODS A retrospective analysis was conducted on the clinical data and survival followup information of 61 patients with stage IIIA NSCLC treated at our hospital from February 2020 to June 2022,and all cases were confirmed as primary(nonrecurrent)diagnoses based on clinical and pathological records.All patients were followed up through outpatient visits or telephone interviews.The follow-up duration ranged from 6 to 51 months with a median follow-up time of 36 months.Overall survival(OS)was defined as the time from the date of pathological diagnosis to death or the last follow-up.Univariate and multivariate Cox regression analyses were performed to examine the relationship between clinical characteristics and OS.Immunohistochemistry was used to detect the expression of CSC markers[octamer-binding transcription factor 4(OCT4),trophoblast cell surface antigen-2(TROP-2),ATP-binding cassette subfamily G member 2(ABCG2),p75 neurotrophin receptor(p75NTR)]in NSCLC,followed by immunohistochemical scoring.The high H-scores of CSC markers,age,and micropapillary components were combined to generate a tumor stemness index(TSI).The Kaplan-Meier method was used to analyze the relationship between CSC markers,TSI,and OS in patients with NSCLC.RESULTS Multivariate Cox regression analysis showed that age[hazard ratio(HR)=1.952,95%confidence interval(CI):1.087-2.481,P=0.029]and micropapillary components(HR=2.716,95%CI:1.259-5.837,P=0.013)were significantly associated with OS.In NSCLC there were 21 cases with high OCT4 H-scores,27 cases with high TROP-2 H-scores,44 cases with high ABCG2 H-scores,and 44 cases with high p75NTR H-scores.In the survival analysis the high OCT4 expression group had a poorer prognosis(P=0.006).Further subtype analysis revealed no statistically significant difference in OS between high and low OCT4 H-score groups in patients with lung squamous cell carcinoma(P=0.457).However,in patients with lung adenocarcinoma high OCT4 expression had significantly poorer OS compared with those with low OCT4 expression(P=0.005).TROP-2,ABCG2,and p75NTR did not significantly affect the prognosis.TSI was significantly associated with OS in patients with NSCLC(HR=2.209,95%CI:1.238-3.681,P=0.027).CONCLUSION Age and micropapillary components were related to OS in patients with stage IIIA NSCLC.High expression of OCT4 and high TSI were associated with poor prognosis.展开更多
Mitral regurgitation (MR) is a highly prevalent valvular heart disease globally,with untreated severe cases demonstrating associations with elevated morbidity,mortality,and adverse cardiovascular outcomes.[1,2]While t...Mitral regurgitation (MR) is a highly prevalent valvular heart disease globally,with untreated severe cases demonstrating associations with elevated morbidity,mortality,and adverse cardiovascular outcomes.[1,2]While transcatheter edge-toedge repair (TEER) has emerged as an alternative option for high surgical risk patients with severe MR,[3,4]severe MR of Carpentier class IIIa (characterized by restricted leaflet motion during both systole and diastole) has been considered a relative contraindication for TEER interventions due to stenosis risk and procedural complexity.[5,6]展开更多
Objective: to report the application of GPIIb/IIIa antagonist (Integrilin) in peri percutaneous transluminal catheter angioplasty and stent implantation in Chinese. Method: Twenty one patients who underwent percutan...Objective: to report the application of GPIIb/IIIa antagonist (Integrilin) in peri percutaneous transluminal catheter angioplasty and stent implantation in Chinese. Method: Twenty one patients who underwent percutaneous transluminal catheter angioplasty(PTCA) and stent implantation were included. After arterial puncture, integrilin was injected at dose of 180ug/kg, and then maintained at 2μg/kg·min for 18 hours. Asprine, plavix(clopidogrel) and heparin were used at the same time. ACT, PT, KPTT and blood routine were routinely monitored. Results: All sheaths were drawn out 2 to 4 hours after the procedure.. There was no severe complication such as hematoma, acute and subacute thrombosis in coronary artery, or thrombocytopenia. ACT returned to less than 150 seconds in 2 hours in 15 patients; in 4 hours in 6 patients. There was no significant difference between the pre and post procedure value of PT and platelet count. KPTT was significantly higher than pre procedure value at 2 hours after the procedure .No recurrence of angina pectoris was observed in the first nine patients within one year follow up, and no restenosis occurred in stents in the five patients who had coronary angiography one year later. Conclusion: Application of GPIIb/IIIa receptor antagonist (Integrilin) in peri percutaneous transluminal catheter angioplasty and stent implantation in combination with aspirin and plavix could significantly reduce the dosage and duration of heparin with benefit of shortening the indwelling time of sheaths, but did not increase risk of bleeding or lead to thrombosis in stent.展开更多
Objectives: To investigate the relation between metastatic potential of salivary adenoid cystic carcinoma (SACC) and tumor cell-platelet adhesion, and the antimetastatic effect of integrin IIb/IIIa inhibitor on SACC. ...Objectives: To investigate the relation between metastatic potential of salivary adenoid cystic carcinoma (SACC) and tumor cell-platelet adhesion, and the antimetastatic effect of integrin IIb/IIIa inhibitor on SACC. Methods: Tumor cell-platelet adhesion of highly metastatic SACC-LM, non-highly metastatic SACC-83 and effect of aspirin, arginine-aspartate (RD), magnesium acetylsalicylate on adhesion were studiedin vitro. Antimetastafic effect of aspirin, RD, magnesium acetysalicylate on experimental metastasis of SACC was observedin vivo. Results: The tumor cell-platelet adhesion was stronger in SACC-LM than in SACC-83. Aspirin, RD and magnesium acetylsalicylate could inhibit the adhesion of SACC-LM at the concentration of 1, 5 and 25 μg/ml. RD can inhibit experimental metastasis of SACC. Conclusion: Metastasis of SACC is related to platelet-tumor cell adhesion, RD could inhibit metastasis of SACC.展开更多
Background Suboptimal myocardial reperfusion is common in patients with ST-segment elevation myocardial infarction （STEMI） undergoing primary percutaneous coronary intervention （PPCI）. Furthermore, it results in i...Background Suboptimal myocardial reperfusion is common in patients with ST-segment elevation myocardial infarction （STEMI） undergoing primary percutaneous coronary intervention （PPCI）. Furthermore, it results in increased infarct size and mortality rates. We performed a meta-analysis to evaluate the role of aspiration thrombectomy （AT） combined with intracoronary administration of glycoprotein IIb/IIIa inhibitors （GPI） in the improvement of myocardial reperfusion and clinical outcomes. Methods PubMed, Embase, Web of Science, and CENTRAL databases were searched for randomized controlled trials （RCTs） investigating combined AT and intracoronary GPI treatment versus AT alone. Outcomes of interest were thrombolysis in myocardial infarction myocardial perfusion grade （TMPG）, infarct size （IS） assessed by cardiac magnetic resonance imaging, left ventricular ejection fraction （LVEF）, major adverse cardiac events （MACE） at short-term （〈 1 month） and long-term （6-12 months） follow-up, and bleeding complications during the hospital stay. Results Eight trials involving 923 patients were included. Compared with AT alone, combined AT and intracoronary GPI significantly increased TMPG 3 flow （RR： 1.15, 95% CI： 1.04 to 1.26）, reduced IS [mean difference （MD）： -3.46, 95% CI： -5.18 to -1.73], and improved LVEF （MD： 1.44, 95% CI： 0.54 to 2.33）. Furthermore, GPI use decreased the risk of MACE at long-term follow-up （RR： 0.60, 95% CI： 0.37 to 0.98）. There was no significant difference between the two groups in the incidence of minor and major bleeding complications. Conclusions Our findings showed that compared with AT alone, combined AT and intracoronary GPI treatment resulted in improved myocardial reperfusion, better cardiac function, and MACE-free survival benefits at the long-term follow-up for patients with STEMI undergoing PPCI.展开更多
基金National Natural Science Foundation of China,Grant/Award Numbers:82270533,U22A6008,81970443National Key Research and Development Program of China,Grant/Award Number:2022YFC3602400+1 种基金Key Health Care Project of National Health,Grant/Award Number:2020ZD05Basic Research Reinforcement Project,Grant/Award Number:JCJQ‐JJ‐1079。
文摘Background:Acute thrombotic events play a major role in various cardio-vascular diseases.Therefore,direct thrombus imaging can be proved beneficial for early diagnosis and prompt therapy of thrombosis.Our study investigated targeted dual‐modality cyclic arginine‐glycine‐aspartic micro bubbles(cRGD‐MBs)for direct imaging of thrombi by fluorescence and ultrasound.Methods:cRGD‐MBs were prepared by mechanical vibration and chemical chelation methods.Results:Coulter counter analysis demonstrated that the cRGD‐MBs were well dispersed,with diameters ranging from 1 to 3μm.They emitted bright red fluorescence under an excitation wavelength of 660 nm.In vivo fluorescence and ultrasound imaging revealed that cRGD‐MBs accumulated at the site of thrombus in the carotid artery with significant fluorescence and ultrasonic signal.Conclusions:This study showed that novel microbubble cRGD‐MBs were successfully synthesized,and that these could potentially be used as contrast agents for immediate diagnosis of acute thrombus in vivo.
文摘BACKGROUND Non-small cell lung cancer(NSCLC)is the most prevalent subtype of lung cancer,accounting for approximately 85%of all lung cancer cases and remaining a major cause of cancer-related mortality worldwide.Despite advances in diagnostic and therapeutic approaches,the incidence and mortality rates of NSCLC continue to rise,especially in low-income and middle-income countries.AIM To investigate the expression of cancer stem cell(CSC)markers and their relationship with the prognosis and survival of patients with stage IIIA NSCLC.METHODS A retrospective analysis was conducted on the clinical data and survival followup information of 61 patients with stage IIIA NSCLC treated at our hospital from February 2020 to June 2022,and all cases were confirmed as primary(nonrecurrent)diagnoses based on clinical and pathological records.All patients were followed up through outpatient visits or telephone interviews.The follow-up duration ranged from 6 to 51 months with a median follow-up time of 36 months.Overall survival(OS)was defined as the time from the date of pathological diagnosis to death or the last follow-up.Univariate and multivariate Cox regression analyses were performed to examine the relationship between clinical characteristics and OS.Immunohistochemistry was used to detect the expression of CSC markers[octamer-binding transcription factor 4(OCT4),trophoblast cell surface antigen-2(TROP-2),ATP-binding cassette subfamily G member 2(ABCG2),p75 neurotrophin receptor(p75NTR)]in NSCLC,followed by immunohistochemical scoring.The high H-scores of CSC markers,age,and micropapillary components were combined to generate a tumor stemness index(TSI).The Kaplan-Meier method was used to analyze the relationship between CSC markers,TSI,and OS in patients with NSCLC.RESULTS Multivariate Cox regression analysis showed that age[hazard ratio(HR)=1.952,95%confidence interval(CI):1.087-2.481,P=0.029]and micropapillary components(HR=2.716,95%CI:1.259-5.837,P=0.013)were significantly associated with OS.In NSCLC there were 21 cases with high OCT4 H-scores,27 cases with high TROP-2 H-scores,44 cases with high ABCG2 H-scores,and 44 cases with high p75NTR H-scores.In the survival analysis the high OCT4 expression group had a poorer prognosis(P=0.006).Further subtype analysis revealed no statistically significant difference in OS between high and low OCT4 H-score groups in patients with lung squamous cell carcinoma(P=0.457).However,in patients with lung adenocarcinoma high OCT4 expression had significantly poorer OS compared with those with low OCT4 expression(P=0.005).TROP-2,ABCG2,and p75NTR did not significantly affect the prognosis.TSI was significantly associated with OS in patients with NSCLC(HR=2.209,95%CI:1.238-3.681,P=0.027).CONCLUSION Age and micropapillary components were related to OS in patients with stage IIIA NSCLC.High expression of OCT4 and high TSI were associated with poor prognosis.
基金supported by the 1·3·5 Project for Disciplines of Excellence from West China Hospital of Sichuan University(ZYGD23021&23HXFH009)Sichuan Science and Technology Program(No.2025ZNSFSC1698)the Sichuan Provincial Cadre Health Research Program(ZH2024-103).
文摘Mitral regurgitation (MR) is a highly prevalent valvular heart disease globally,with untreated severe cases demonstrating associations with elevated morbidity,mortality,and adverse cardiovascular outcomes.[1,2]While transcatheter edge-toedge repair (TEER) has emerged as an alternative option for high surgical risk patients with severe MR,[3,4]severe MR of Carpentier class IIIa (characterized by restricted leaflet motion during both systole and diastole) has been considered a relative contraindication for TEER interventions due to stenosis risk and procedural complexity.[5,6]
文摘Objective: to report the application of GPIIb/IIIa antagonist (Integrilin) in peri percutaneous transluminal catheter angioplasty and stent implantation in Chinese. Method: Twenty one patients who underwent percutaneous transluminal catheter angioplasty(PTCA) and stent implantation were included. After arterial puncture, integrilin was injected at dose of 180ug/kg, and then maintained at 2μg/kg·min for 18 hours. Asprine, plavix(clopidogrel) and heparin were used at the same time. ACT, PT, KPTT and blood routine were routinely monitored. Results: All sheaths were drawn out 2 to 4 hours after the procedure.. There was no severe complication such as hematoma, acute and subacute thrombosis in coronary artery, or thrombocytopenia. ACT returned to less than 150 seconds in 2 hours in 15 patients; in 4 hours in 6 patients. There was no significant difference between the pre and post procedure value of PT and platelet count. KPTT was significantly higher than pre procedure value at 2 hours after the procedure .No recurrence of angina pectoris was observed in the first nine patients within one year follow up, and no restenosis occurred in stents in the five patients who had coronary angiography one year later. Conclusion: Application of GPIIb/IIIa receptor antagonist (Integrilin) in peri percutaneous transluminal catheter angioplasty and stent implantation in combination with aspirin and plavix could significantly reduce the dosage and duration of heparin with benefit of shortening the indwelling time of sheaths, but did not increase risk of bleeding or lead to thrombosis in stent.
基金the National Natural Science Foundation of China (No. 39270723).
文摘Objectives: To investigate the relation between metastatic potential of salivary adenoid cystic carcinoma (SACC) and tumor cell-platelet adhesion, and the antimetastatic effect of integrin IIb/IIIa inhibitor on SACC. Methods: Tumor cell-platelet adhesion of highly metastatic SACC-LM, non-highly metastatic SACC-83 and effect of aspirin, arginine-aspartate (RD), magnesium acetylsalicylate on adhesion were studiedin vitro. Antimetastafic effect of aspirin, RD, magnesium acetysalicylate on experimental metastasis of SACC was observedin vivo. Results: The tumor cell-platelet adhesion was stronger in SACC-LM than in SACC-83. Aspirin, RD and magnesium acetylsalicylate could inhibit the adhesion of SACC-LM at the concentration of 1, 5 and 25 μg/ml. RD can inhibit experimental metastasis of SACC. Conclusion: Metastasis of SACC is related to platelet-tumor cell adhesion, RD could inhibit metastasis of SACC.
文摘Background Suboptimal myocardial reperfusion is common in patients with ST-segment elevation myocardial infarction （STEMI） undergoing primary percutaneous coronary intervention （PPCI）. Furthermore, it results in increased infarct size and mortality rates. We performed a meta-analysis to evaluate the role of aspiration thrombectomy （AT） combined with intracoronary administration of glycoprotein IIb/IIIa inhibitors （GPI） in the improvement of myocardial reperfusion and clinical outcomes. Methods PubMed, Embase, Web of Science, and CENTRAL databases were searched for randomized controlled trials （RCTs） investigating combined AT and intracoronary GPI treatment versus AT alone. Outcomes of interest were thrombolysis in myocardial infarction myocardial perfusion grade （TMPG）, infarct size （IS） assessed by cardiac magnetic resonance imaging, left ventricular ejection fraction （LVEF）, major adverse cardiac events （MACE） at short-term （〈 1 month） and long-term （6-12 months） follow-up, and bleeding complications during the hospital stay. Results Eight trials involving 923 patients were included. Compared with AT alone, combined AT and intracoronary GPI significantly increased TMPG 3 flow （RR： 1.15, 95% CI： 1.04 to 1.26）, reduced IS [mean difference （MD）： -3.46, 95% CI： -5.18 to -1.73], and improved LVEF （MD： 1.44, 95% CI： 0.54 to 2.33）. Furthermore, GPI use decreased the risk of MACE at long-term follow-up （RR： 0.60, 95% CI： 0.37 to 0.98）. There was no significant difference between the two groups in the incidence of minor and major bleeding complications. Conclusions Our findings showed that compared with AT alone, combined AT and intracoronary GPI treatment resulted in improved myocardial reperfusion, better cardiac function, and MACE-free survival benefits at the long-term follow-up for patients with STEMI undergoing PPCI.
