Glycosylphosphatidylinositol(GPI)anchoring represents a fundamental post-translational modification in eukaryotic cells.In fungi,this modification facilitates diverse biological functions through protein targeting to ...Glycosylphosphatidylinositol(GPI)anchoring represents a fundamental post-translational modification in eukaryotic cells.In fungi,this modification facilitates diverse biological functions through protein targeting to the cell wall,yet research on its roles in plant pathogenic fungi remains limited.This study elucidates the function of GPI anchoring in the maize fungal pathogen Cochlibolus heterostrophus.The research demonstrates widespread accumulation of GPI-anchored proteins in hyphae,appressorium and infection hyphae of C.heterostrophus.Deletion of ChGPI7,encoding a crucial enzyme in GPI anchor biosynthesis,substantially reduced vegetative growth,conidiation,and virulence through impaired appressorium formation and invasive growth.The ΔChgpi7 mutants exhibited marked deficiencies in cell wall integrity,leading to decreased stress resistance.Both ChGPI7 deletion and hydro fluoric acid(HF)pyridine treatment eliminated cell wall GPI-anchored proteins and exposed chitin,indicating that GPI-anchored proteins shield chitin from host immune recognition.Analysis identified 124 predicted GPI-anchored proteins in C.heterostrophus,including the putative cell wall glycoprotein ChFEM1.The deletion of ChFEM1 similarly reduced virulence and compromised infection structures and cell wall integrity.Additionally,ChGPI7 influenced both the cell wall localization and protein abundance of ChFEM1.These findings demonstrate that GPI anchoring mediates cell wall integrity and immune evasion during C.heterostrophus infection.展开更多
In this article, we establish the route taken by the author, and his research group, to bring differential flatness to the realm of active disturbance rejection control （ADRC）. This avenue entitled： 1） generalized...In this article, we establish the route taken by the author, and his research group, to bring differential flatness to the realm of active disturbance rejection control （ADRC）. This avenue entitled： 1） generalized proportional integral observers （GPIO）, as natural state and disturbance observers for fiat systems, 2） generalized proportional integral （GPI） control, provided with extra integrations, to produce a modular controller known as flat filters （FF＇s） and, finally, 3） the establishing of an equivalence of observer based ADRC with FF＇s. The context is that of pure integration systems. The obtained controllers depend only on the order of the flat system and they are to be directly used on the basis of the available flat output signal in a universal, modular, fashion. The map is complemented with the relevant references where the intermediate techniques were illustrated and developed, over the years, in connection with laboratory experimental implementations.展开更多
基金supported by the Fundamental Research Funds for the Central Universities,China(2021ZKPY007).
文摘Glycosylphosphatidylinositol(GPI)anchoring represents a fundamental post-translational modification in eukaryotic cells.In fungi,this modification facilitates diverse biological functions through protein targeting to the cell wall,yet research on its roles in plant pathogenic fungi remains limited.This study elucidates the function of GPI anchoring in the maize fungal pathogen Cochlibolus heterostrophus.The research demonstrates widespread accumulation of GPI-anchored proteins in hyphae,appressorium and infection hyphae of C.heterostrophus.Deletion of ChGPI7,encoding a crucial enzyme in GPI anchor biosynthesis,substantially reduced vegetative growth,conidiation,and virulence through impaired appressorium formation and invasive growth.The ΔChgpi7 mutants exhibited marked deficiencies in cell wall integrity,leading to decreased stress resistance.Both ChGPI7 deletion and hydro fluoric acid(HF)pyridine treatment eliminated cell wall GPI-anchored proteins and exposed chitin,indicating that GPI-anchored proteins shield chitin from host immune recognition.Analysis identified 124 predicted GPI-anchored proteins in C.heterostrophus,including the putative cell wall glycoprotein ChFEM1.The deletion of ChFEM1 similarly reduced virulence and compromised infection structures and cell wall integrity.Additionally,ChGPI7 influenced both the cell wall localization and protein abundance of ChFEM1.These findings demonstrate that GPI anchoring mediates cell wall integrity and immune evasion during C.heterostrophus infection.
文摘In this article, we establish the route taken by the author, and his research group, to bring differential flatness to the realm of active disturbance rejection control （ADRC）. This avenue entitled： 1） generalized proportional integral observers （GPIO）, as natural state and disturbance observers for fiat systems, 2） generalized proportional integral （GPI） control, provided with extra integrations, to produce a modular controller known as flat filters （FF＇s） and, finally, 3） the establishing of an equivalence of observer based ADRC with FF＇s. The context is that of pure integration systems. The obtained controllers depend only on the order of the flat system and they are to be directly used on the basis of the available flat output signal in a universal, modular, fashion. The map is complemented with the relevant references where the intermediate techniques were illustrated and developed, over the years, in connection with laboratory experimental implementations.
