[Objectives] This study was conducted to analyze the infection ability of Rhizobium radiobacter from Rosa chinensis to peach tree,so as to provide a theoretical basis for the identification and control of crown gall o...[Objectives] This study was conducted to analyze the infection ability of Rhizobium radiobacter from Rosa chinensis to peach tree,so as to provide a theoretical basis for the identification and control of crown gall of peach. [Methods]With peach variety ＂ Chiyohime＂ as an experimental material,R. radiobacter GOU1 was extracted from crown gall tissue of R. chinensis susceptible to crown gall,and its infection ability to peach tree was studied by in-vitro dip method.[Results]Stem segments of peach tree were inoculated with R. radiobacter GOU1 by in-vitro dip method. Calli grew rapidly after inoculation,and 28 d later,the infection rate of R. radiobacter GOU1 was 50%,indicating that R. radiobacter GOU1 could infect peach tree easier. The infection on stem segments of peach tree could be detected in the fourth week by in-vitro dip method. Culturing peach stem segments in MS medium containing 10 μM/L NAA and 10 μM/L BAP in vitro is beneficial to survival and could promote growth of callus. [Conclusions]The detection rate of R. radiobacter GOU1 infection on stem segments of peach tree did not change from the fourth week to the sixth week. Therefore,in future studies,the detection of R. radiobacter infection on inoculated stem segments of peach tree could be performed after 4 weeks of culture rather than 6 weeks of culture.展开更多
基金Supported by Natural Science Foundation of China(31260461)Guangxi Innovation Team Nanning Test Station Project of National Modern Agriculture Technology System(nycytxcxtd-03-12-3,nycytxcxtd-04-18)Undergraduate Teaching Reform Project of Higher Education of Guangxi Province(2017JGB132)
文摘[Objectives] This study was conducted to analyze the infection ability of Rhizobium radiobacter from Rosa chinensis to peach tree,so as to provide a theoretical basis for the identification and control of crown gall of peach. [Methods]With peach variety ＂ Chiyohime＂ as an experimental material,R. radiobacter GOU1 was extracted from crown gall tissue of R. chinensis susceptible to crown gall,and its infection ability to peach tree was studied by in-vitro dip method.[Results]Stem segments of peach tree were inoculated with R. radiobacter GOU1 by in-vitro dip method. Calli grew rapidly after inoculation,and 28 d later,the infection rate of R. radiobacter GOU1 was 50%,indicating that R. radiobacter GOU1 could infect peach tree easier. The infection on stem segments of peach tree could be detected in the fourth week by in-vitro dip method. Culturing peach stem segments in MS medium containing 10 μM/L NAA and 10 μM/L BAP in vitro is beneficial to survival and could promote growth of callus. [Conclusions]The detection rate of R. radiobacter GOU1 infection on stem segments of peach tree did not change from the fourth week to the sixth week. Therefore,in future studies,the detection of R. radiobacter infection on inoculated stem segments of peach tree could be performed after 4 weeks of culture rather than 6 weeks of culture.
