One feature of Golding' s works is to reveal the theme-the evil nature of man,through the characters' mystical experience.This is obvious in Darkness Visible and Lord of the Flies.This paper tries to find out ...One feature of Golding' s works is to reveal the theme-the evil nature of man,through the characters' mystical experience.This is obvious in Darkness Visible and Lord of the Flies.This paper tries to find out the resource for the writer to use mystical experience through biological approach.展开更多
Beyond the stories,Golding spends much effort on an issue called"impotence of language"in his works,especially Lord of the Flies and Darkness Visible.Thus Golding's language view is worthwhile to researc...Beyond the stories,Golding spends much effort on an issue called"impotence of language"in his works,especially Lord of the Flies and Darkness Visible.Thus Golding's language view is worthwhile to research.With help of Lacan's theory of signifier and signified,this paper made a primary glance about Golding's language view within the two novels.展开更多
The Mailong gold deposit is located in the eastern section of the East Kunlun orogenic belt and is one of the recently discovered important gold polymetallic deposits in the Qinhai Gouli region.The primary host rocks ...The Mailong gold deposit is located in the eastern section of the East Kunlun orogenic belt and is one of the recently discovered important gold polymetallic deposits in the Qinhai Gouli region.The primary host rocks of the Mailong gold deposit consist of intermediate-acid intrusive rocks from the Varisian and Indosinian periods,as well as the Precambrian Jinshuikou Group,with mineralization controlled by northeast and northwest faults.The alteration of the host rocks is mainly characterized by silicification,sericitization,chloritization,and carbonatization.Based on the cross-cutting relationships of the veins,the hydrothermal mineralization of the gold deposit can be categorized into three stages:the quartz-pyrite stage,the quartzpolymetallic sulfide stage,and the quartz-carbonate stage.Microthermometry of fluid inclusions indicates that the Mailong gold deposit belongs to a low-density(0.73–0.86 g/cm3),medium-temperature(240–340℃),and medium-salinity(4.01–10.74 wt%NaCl)NaCl-CO2-H2O fluid system.The C-H-O isotopic analysis suggests that the mineralizing fluids is derived from magmatic water,with later contributions from atmospheric precipitation.In-situ S isotopic results indicate that the mineralizing materials mainly derive from igneous rocks.A comprehensive analysis concludes that the Mailong gold deposit is a mesothermal hydrothermal vein-type gold deposit controlled by structural factors.展开更多
Rational design of nanozymes with enhanced catalytic efficiency remains a central challenge in the development of artificial enzymes.Herein,we report the construction of ultrasmall gold nanoclusterbased nanoassemblies...Rational design of nanozymes with enhanced catalytic efficiency remains a central challenge in the development of artificial enzymes.Herein,we report the construction of ultrasmall gold nanoclusterbased nanoassemblies(Dp-Au NCs@Fe^(2+)) through the coordination of Fe^(2+) ions by a dopa-containing peptidomimetic ligand(Dp CDp).This nanoarchitecture simultaneously integrates catalytically active gold cores and redox-active Fe^(2+)centers,bridged by Dp CDp to facilitate directional electron transfer.Comprehensive spectroscopic and kinetic analyses reveal that Dp CDp promotes efficient charge migration from the Au core to surface-bound Fe^(2+),significantly enhancing H_(2)O_(2)-mediated peroxidase-like activity.Compared to bare Dp-Au NCs,Dp-Au NCs@Fe^(2+) display a 4.3-fold improvement in detection sensitivity,a 6.7-fold increase in catalytic efficiency,and markedly stronger hydroxyl radical generation.Mechanistically,this activity stems from a synergistic triad:direct H_(2)O_(2) oxidation at gold surfaces,radical generation at Fe^(2+) sites,and Dp CDp-facilitated electron shuttling.This work presents a robust strategy for nanozyme enhancement via electronic and structural co-engineering,offering valuable insights for the future design of bioinspired catalytic systems.展开更多
The recovery of gold from waste electronic and electric equipment(WEEE) has gained great attention with the increased number of WEEE,because it can largely alleviate the pressure on the environment and resources.Coval...The recovery of gold from waste electronic and electric equipment(WEEE) has gained great attention with the increased number of WEEE,because it can largely alleviate the pressure on the environment and resources.Covalent organic frameworks(COFs) are ideal adsorbents for gold recovery owing to their large surface area,good stability,easily functionalized ability,periodic structures,and definitive nanopores.Herein,a cyano-functionalized COF(COF-CN) with high crystallinity was large-scale prepared under mild conditions for the recovery of gold.The introduction of cyano groups enable COF-CN to exhibit excellent gold recovery performance,which possesses fast adsorption kinetics,high cycling stability,and adsorption capacity up to 663.67 mg/g.Excitingly,COF-CN showed extremely high selectivity for gold ions,even in the presence of various competing cations and anions.The COF-CN maintained excellent selectivity and removal efficiency in gold recovery experiments from WEEE.The facile synthesis of COF-CN and its outstanding selectivity in actual samples make it an attractive opportunity for practical gold recovery.展开更多
The field of nanomedicine has been revolutionized by the concept of immunogenic cell death(ICD)-enhanced cancer therapy,which holds immense promise for the efficient treatment of cancer.However,precise delivery of ICD...The field of nanomedicine has been revolutionized by the concept of immunogenic cell death(ICD)-enhanced cancer therapy,which holds immense promise for the efficient treatment of cancer.However,precise delivery of ICD inducer is severely hindered by complex biological barriers.How to design and build intelligent nanoplatform for adaptive and dynamic cancer therapy remains a big challenge.Herein,this article presents the design and preparation of CD44-targeting and ZIF-8 gated gold nanocage(Au@ZH) for programmed delivery of the 1,2-diaminocyclohexane-Pt(Ⅱ)(DACHPt) as ICD inducer.After actively targeting the CD44 on the surface of 4T1 tumor cell,this Pt-Au@ZH can be effectively endocytosed by the 4T1 cell and release the DACHPt in tumor acidic environment,resulting in ICD effect and superior antitumor efficacy both in vitro and in vivo in the presence of mild 808 nm laser irradiation.By integration of internal and external stimuli intelligently,this programmed nanoplatform is poised to become a cornerstone in the pursuit of effective and targeted cancer therapy in the foreseeable future.展开更多
Chinese New Year is a time of vibrant energy and delightful surprises.Streets across China glow with red and gold decorations,while the air carries the scent of freshly steamed pastries and a wide variety of sweet tre...Chinese New Year is a time of vibrant energy and delightful surprises.Streets across China glow with red and gold decorations,while the air carries the scent of freshly steamed pastries and a wide variety of sweet treats.As the Year of the Horse gallops in,it brings a surge of vitality to the communities.Beyond fireworks,decorations,and dragon dances.展开更多
Microwave roasting self-leaching is an innovative method for recovering gold from high-sulfur refractory gold concentrates,without using deadly toxic cyanide reagents.However,the mechanism of gold self-leaching,which ...Microwave roasting self-leaching is an innovative method for recovering gold from high-sulfur refractory gold concentrates,without using deadly toxic cyanide reagents.However,the mechanism of gold self-leaching,which relies on lixiviants prepared using volatilized sulfur obtained from roasting,has not been fully elucidated.This study employs the response surface methodology to optimize processing parameters,resulting in an increased gold extraction rate of 96.18%.Analytical factorization and the Tafel curve indicate that CuSO_(4) and NH_(3)·H_(2)O significantly influence the self-leaching process.Furthermore,X-ray photoelectron spectroscopy(XPS)analysis reveals that S^(2−),S_(2)^(2−),polysulfides(S_(n)^(2−)),and thiosulfate(S_(2)O_(3)^(2−))are involved in the gold leaching reaction,with S^(2−),S_(2)^(2−),and S_(n)^(2−) serving as primary ligands for gold complexation.The role of S_(2)O_(3)^(2−) in the early stages of the gold-leaching reaction is also noteworthy.The copper–ammonia complex catalyzes the self-leaching gold reaction;however,an improper addition ratio can lead to copper-sulfur compound precipitates,reducing the extraction rate.展开更多
Highlights By conjugating the same anti-N monoclonal antibody(mAb4-mAb1)with colloidal gold or fluorescent microspheres,this study developed two rapid point-of-care antigen immunochromatographic strips for the detecti...Highlights By conjugating the same anti-N monoclonal antibody(mAb4-mAb1)with colloidal gold or fluorescent microspheres,this study developed two rapid point-of-care antigen immunochromatographic strips for the detection of porcine deltacoronavirus.The fluorescent microsphere-based lateral flow test strip demonstrated a sensitivity of 10^(1.7)TCID_(50)/0.1 mL,which is fourfold higher than that of the colloidal gold-based assay.Porcine deltacoronavirus(PDCoV)is a recently identified enteric coronavirus that causes an acute infectious disease in piglets,leading to diarrhea,vomiting,dehydration,and mortality(Hu et al.2015).展开更多
Gold ores in the Jiaozhou region of China are characterized by their abundant reserves,low grade,fine dissemination,and chal-lenges in upgrading.Froth flotation,with xanthate as the collector,is a commonly employed me...Gold ores in the Jiaozhou region of China are characterized by their abundant reserves,low grade,fine dissemination,and chal-lenges in upgrading.Froth flotation,with xanthate as the collector,is a commonly employed method for enriching auriferous pyrite from these ores.This study aimed to develop a more efficient flotation process by utilizing cavitation nanobubbles for a low-grade gold ore.Batch flotation tests demonstrated that nanobubbles significantly enhanced the flotation performance of auriferous pyrite,as evidenced by improved concentrate S and Au grades and their recoveries.The mechanisms underlying this enhancement were explored by investigat-ing surface nanobubble(SNB)formation,bulk nanobubble(BNB)attachment to hydrophobic pyrite surfaces,and nanobubble-induced agglomeration using atomic force microscopy(AFM)and focused beam reflectance measurement(FBRM).The results revealed that nan-obubble coverage on the pyrite surface is a critical factor influencing surface hydrophobicity and agglomeration.SNBs exhibited higher coverage on pyrite surfaces with increased surface hydrophobicity,flow rate,and cavitation time.Similarly,BNB attachment on pyrite surfaces was significantly increased with surface hydrophobicity and cavitation time.Enhanced surface hydrophobicity,along with higher flow rates and cavitation times,promoted pyrite particle agglomeration owing to the increased nanobubble coverage,ultimately leading to improved flotation performance.展开更多
One of the major challenges in the application of microbially induced carbonate precipitation(MICP)is achieving"bacteria freedom",as it necessitates a substantial volume of bacterial solutions.Nevertheless,b...One of the major challenges in the application of microbially induced carbonate precipitation(MICP)is achieving"bacteria freedom",as it necessitates a substantial volume of bacterial solutions.Nevertheless,both insitu bacterial cultivation and transportation of bacterial solutions have proven to be inefficient.In this study,we suggested the utilization of bacteria in the form of dry powder,enabling easy on-site activation and achieving a relatively high urease activity.We conducted MICP curing experiments on gold mine tailings(GMT)using steel slag(SS)as an additive.The results showed that the average unconfined compressive strength(UCS)values of the tailings treated with MICP and MICP+SS reached 0.51 and 0.71 MPa,respectively.In addition,the average leaching reduction rates of Cu,Pb,Cr,Zn,and T-CN in GMT after MICP treatment reached 98.54%,100%,70.94%,59.25%,and 98.02%,respectively,and the average reduction rates after MICP+SS treatment reached 98.77%,100%,88.03%,72.59%,and 98.63%,respectively.SEM,XRD,FT-IR analyses,and ultra-deep field microscopy results confirmed that the MICP treatment produced calcite-based calcium carbonate that filled the inter-tailing pores and cemented them together,and the hydration mechanism was the main reason for the increased curing efficiency of SS.Our research findings demonstrate that bacterial powder can efficiently achieve the objectives of heavy metal removal and tailing solidification.This approach can substantially de-crease the expenses associated with bacterial cultivation and solution transportation,thereby playing a crucial role in advancing the practical implementation of MICP.展开更多
To treat cancer and inhibit its metastasis to the greatest extent,we proposed to develop an Au(III)agent to induce immunogenic cell death(ICD)and establish long-term immunity.To this end,we optimized a series of Au(II...To treat cancer and inhibit its metastasis to the greatest extent,we proposed to develop an Au(III)agent to induce immunogenic cell death(ICD)and establish long-term immunity.To this end,we optimized a series of Au(III)2-benzoylpyridine thiosemicarbazone complexes to obtain an Au(III)agent(5b)with excellent cytotoxicity to cancer.The results show that 5b effectively inhibits tumor growth and its metastasis in vivo.Interestingly,we revealed a new mechanism of 5b inhibiting tumor growth and metastasis:5b releases ICD-related damage-associated molecular patterns(DAMPs),such as calreticulin(CRT),ATP and high mobility group box 1(HMGB1)by inducing endoplasmic reticulum stress(ERS)and mitochondrial dysfunction,which then stimulated an antitumor CD8^(+)T cell response and Foxp^(3+)T cell depletion,thus establishing long-action antitumor immunity.展开更多
Metal-organic framework(MOF)nanostructures have emerged as a prominent class of materials in the advancement of electrochemical sensors.The rational design of bimetallic MOF-functionalized microelectrode is of importa...Metal-organic framework(MOF)nanostructures have emerged as a prominent class of materials in the advancement of electrochemical sensors.The rational design of bimetallic MOF-functionalized microelectrode is of importance for improv-ing the electrochemical performance but still in great challenge.In this work,the bimetallic FeCo-MOF nanostructures were assembled onto a gold disk ultramicroelectrode(Au UME,5.2μm in diameter)via an in-situ electrodeposition method,which enhanced the sensitive detection of epinephrine(EP).The in-situ electrodeposited FeCo-MOF exhibited a character-istic nanoflower-like morphology and was uniformly dispersed on the Au UME.The FeCo-MOF/Au UME demonstrated excellent electrochemical performance on the detection of EP with a high sensitivity of 36.93μA·μmol^(-1)·L·cm^(-2)and a low detection limit of 1.28μmol·L^(-1).It can be attributed to the nonlinear diffusion of EP onto the ultra-micro working substrate,coupled with synergistical catalytic activity of the bimetallic Fe,Co within MOF structure.Furthermore,the FeCo-MOF/Au UME has been successful applied to the analysis of EP in human serum samples,yielding high recovery rates.These results not only contribute to the expansion of the research area of electrochemical sensors,but also provide novel insights and directions into the development of high-performance MOF-based electrochemical sensors.展开更多
Dopamine(DA)is a vital neurotransmitter,and accurate detection of its concentration is critical for both clinical diagnos-tics and neuroscience research.Due to its electrochemical activity,DA is commonly detected usin...Dopamine(DA)is a vital neurotransmitter,and accurate detection of its concentration is critical for both clinical diagnos-tics and neuroscience research.Due to its electrochemical activity,DA is commonly detected using electrochemical methods,which are favored for their simplicity,fast response time,and suitability for in vivo analysis.In this work,a highly sensitive DA electrochemical sensor was developed using an Au@MoS_(2)composite,created by modifying molybdenum disulfide(MoS_(2))nanosheets with gold nanoparticles through HAuCl_(4) reduction,and it was aimed at enhancing DA adsorption and improving detection performance.Scanning Electron Microscopy(SEM),transmission electron microscopy(TEM),Energy Dispersive Spectroscopy(EDS),X-ray photoelectron spectroscopy(XPS)and X-ray Diffraction(XRD)confirmed the suc-cessful synthesis of Au@MoS_(2)and the uniform distribution of gold nanoparticles across the MoS_(2)nanosheets.Then,the electrochemical characterization demonstrated that the Au@MoS_(2)/GCE exhibited distinct oxidation peaks in a 10μmol·L^(-1)DA solution,with significantly enhanced electrochemical activity compared to both unmodified GCE and pristine MoS_(2).Furthermore,differential pulse voltammetry(DPV)further revealed a strong linear relationship between DA concentration and the current response in the range of 800 nmol·L^(-1)to 10μmol·L^(-1),with a low detection limit(LOD)of 78.9 nmol·L^(-1)(S/N=3).Additionally,the sensor showed excellent selectivity against other interfering substances.Moreover,the laser-induced Au@MoS_(2)(LIAu@MoS_(2)),with its abundance of negatively charged surface defects,enabled the ultra-sensitive detection of the ultra-low concentrations of DA.In conclusion,the successfully fabricated Au@MoS_(2)based sensor offers advantages such as low cost,ease of operation,and scalability,making it a promising candidate for biosensing applications due to its enhanced DA detection capabilities.展开更多
A new gold self-relay catalytic annulation/nucleophilic substitution cascade of 1,3-enyne acetates with cyclic ether acetals is reported,enabling highly diastereoselective access to cyclic etherified cyclopentenones w...A new gold self-relay catalytic annulation/nucleophilic substitution cascade of 1,3-enyne acetates with cyclic ether acetals is reported,enabling highly diastereoselective access to cyclic etherified cyclopentenones with cyclic quaternary centers in moderate to good yields and>19∶1 dr.This catalysis enables the direct construction of two types of carboncyclic skeletons by adjusting the olefin types of 1,3-enyne acetates.When 1,3-enyne acetates bearing a cyclic alkene unit were used,5~6 fused bicarbocyclic products were diastereoselectively synthesized,whereas the reaction of acyclic 1,3-enyne acetates resulted in five-memebered carbocyclic framework.Notably,cyclic ether acetals are commonly used as protecting groups in traditional multistep organic syntheses,and in this reaction,such reagents serve as electrophilic cyclic ether precursors,achieving new uses for old reagents.The current method demonstrates good functional group compatibility,a broad substrate scope and high diastereoselectivity,providing a new synthetic strategy toward functionalized cyclopentenones.展开更多
文摘One feature of Golding' s works is to reveal the theme-the evil nature of man,through the characters' mystical experience.This is obvious in Darkness Visible and Lord of the Flies.This paper tries to find out the resource for the writer to use mystical experience through biological approach.
文摘Beyond the stories,Golding spends much effort on an issue called"impotence of language"in his works,especially Lord of the Flies and Darkness Visible.Thus Golding's language view is worthwhile to research.With help of Lacan's theory of signifier and signified,this paper made a primary glance about Golding's language view within the two novels.
基金Supported by Qinghai Provincial Geological Exploration Special Fund Project(No.2023085029KY004).
文摘The Mailong gold deposit is located in the eastern section of the East Kunlun orogenic belt and is one of the recently discovered important gold polymetallic deposits in the Qinhai Gouli region.The primary host rocks of the Mailong gold deposit consist of intermediate-acid intrusive rocks from the Varisian and Indosinian periods,as well as the Precambrian Jinshuikou Group,with mineralization controlled by northeast and northwest faults.The alteration of the host rocks is mainly characterized by silicification,sericitization,chloritization,and carbonatization.Based on the cross-cutting relationships of the veins,the hydrothermal mineralization of the gold deposit can be categorized into three stages:the quartz-pyrite stage,the quartzpolymetallic sulfide stage,and the quartz-carbonate stage.Microthermometry of fluid inclusions indicates that the Mailong gold deposit belongs to a low-density(0.73–0.86 g/cm3),medium-temperature(240–340℃),and medium-salinity(4.01–10.74 wt%NaCl)NaCl-CO2-H2O fluid system.The C-H-O isotopic analysis suggests that the mineralizing fluids is derived from magmatic water,with later contributions from atmospheric precipitation.In-situ S isotopic results indicate that the mineralizing materials mainly derive from igneous rocks.A comprehensive analysis concludes that the Mailong gold deposit is a mesothermal hydrothermal vein-type gold deposit controlled by structural factors.
基金supported by the National Natural Science Foundation of China (Nos.22177133,22278438)。
文摘Rational design of nanozymes with enhanced catalytic efficiency remains a central challenge in the development of artificial enzymes.Herein,we report the construction of ultrasmall gold nanoclusterbased nanoassemblies(Dp-Au NCs@Fe^(2+)) through the coordination of Fe^(2+) ions by a dopa-containing peptidomimetic ligand(Dp CDp).This nanoarchitecture simultaneously integrates catalytically active gold cores and redox-active Fe^(2+)centers,bridged by Dp CDp to facilitate directional electron transfer.Comprehensive spectroscopic and kinetic analyses reveal that Dp CDp promotes efficient charge migration from the Au core to surface-bound Fe^(2+),significantly enhancing H_(2)O_(2)-mediated peroxidase-like activity.Compared to bare Dp-Au NCs,Dp-Au NCs@Fe^(2+) display a 4.3-fold improvement in detection sensitivity,a 6.7-fold increase in catalytic efficiency,and markedly stronger hydroxyl radical generation.Mechanistically,this activity stems from a synergistic triad:direct H_(2)O_(2) oxidation at gold surfaces,radical generation at Fe^(2+) sites,and Dp CDp-facilitated electron shuttling.This work presents a robust strategy for nanozyme enhancement via electronic and structural co-engineering,offering valuable insights for the future design of bioinspired catalytic systems.
基金financially supported by the National Natural Science Foundation of China (No.51972302)。
文摘The recovery of gold from waste electronic and electric equipment(WEEE) has gained great attention with the increased number of WEEE,because it can largely alleviate the pressure on the environment and resources.Covalent organic frameworks(COFs) are ideal adsorbents for gold recovery owing to their large surface area,good stability,easily functionalized ability,periodic structures,and definitive nanopores.Herein,a cyano-functionalized COF(COF-CN) with high crystallinity was large-scale prepared under mild conditions for the recovery of gold.The introduction of cyano groups enable COF-CN to exhibit excellent gold recovery performance,which possesses fast adsorption kinetics,high cycling stability,and adsorption capacity up to 663.67 mg/g.Excitingly,COF-CN showed extremely high selectivity for gold ions,even in the presence of various competing cations and anions.The COF-CN maintained excellent selectivity and removal efficiency in gold recovery experiments from WEEE.The facile synthesis of COF-CN and its outstanding selectivity in actual samples make it an attractive opportunity for practical gold recovery.
基金financially supported by the Natural Science Foundation of Jiangsu Province (No.BK20200709)the Natural Science Foundation of China (Nos.62288102,32201127 and 82270113)+2 种基金the Natural Science Foundation of Guangdong Province (No.2023A1515011386)the Natural Science Foundation of the Jiangsu Higher Education Institutes (No.20KJB430031)the startup fund from Nanjing Tech University,and Disciplinary Fund of School of Pharmaceutical Sciences (2024)。
文摘The field of nanomedicine has been revolutionized by the concept of immunogenic cell death(ICD)-enhanced cancer therapy,which holds immense promise for the efficient treatment of cancer.However,precise delivery of ICD inducer is severely hindered by complex biological barriers.How to design and build intelligent nanoplatform for adaptive and dynamic cancer therapy remains a big challenge.Herein,this article presents the design and preparation of CD44-targeting and ZIF-8 gated gold nanocage(Au@ZH) for programmed delivery of the 1,2-diaminocyclohexane-Pt(Ⅱ)(DACHPt) as ICD inducer.After actively targeting the CD44 on the surface of 4T1 tumor cell,this Pt-Au@ZH can be effectively endocytosed by the 4T1 cell and release the DACHPt in tumor acidic environment,resulting in ICD effect and superior antitumor efficacy both in vitro and in vivo in the presence of mild 808 nm laser irradiation.By integration of internal and external stimuli intelligently,this programmed nanoplatform is poised to become a cornerstone in the pursuit of effective and targeted cancer therapy in the foreseeable future.
文摘Chinese New Year is a time of vibrant energy and delightful surprises.Streets across China glow with red and gold decorations,while the air carries the scent of freshly steamed pastries and a wide variety of sweet treats.As the Year of the Horse gallops in,it brings a surge of vitality to the communities.Beyond fireworks,decorations,and dragon dances.
基金supported by the National Natural Science Foundation of China(No.51974016).
文摘Microwave roasting self-leaching is an innovative method for recovering gold from high-sulfur refractory gold concentrates,without using deadly toxic cyanide reagents.However,the mechanism of gold self-leaching,which relies on lixiviants prepared using volatilized sulfur obtained from roasting,has not been fully elucidated.This study employs the response surface methodology to optimize processing parameters,resulting in an increased gold extraction rate of 96.18%.Analytical factorization and the Tafel curve indicate that CuSO_(4) and NH_(3)·H_(2)O significantly influence the self-leaching process.Furthermore,X-ray photoelectron spectroscopy(XPS)analysis reveals that S^(2−),S_(2)^(2−),polysulfides(S_(n)^(2−)),and thiosulfate(S_(2)O_(3)^(2−))are involved in the gold leaching reaction,with S^(2−),S_(2)^(2−),and S_(n)^(2−) serving as primary ligands for gold complexation.The role of S_(2)O_(3)^(2−) in the early stages of the gold-leaching reaction is also noteworthy.The copper–ammonia complex catalyzes the self-leaching gold reaction;however,an improper addition ratio can lead to copper-sulfur compound precipitates,reducing the extraction rate.
基金financially supported by the National Key Research and Development Program of China(2021YFF0703600)。
文摘Highlights By conjugating the same anti-N monoclonal antibody(mAb4-mAb1)with colloidal gold or fluorescent microspheres,this study developed two rapid point-of-care antigen immunochromatographic strips for the detection of porcine deltacoronavirus.The fluorescent microsphere-based lateral flow test strip demonstrated a sensitivity of 10^(1.7)TCID_(50)/0.1 mL,which is fourfold higher than that of the colloidal gold-based assay.Porcine deltacoronavirus(PDCoV)is a recently identified enteric coronavirus that causes an acute infectious disease in piglets,leading to diarrhea,vomiting,dehydration,and mortality(Hu et al.2015).
基金support from the National Natural Science Foundation of China(No.52204274)the Shandong Provincial Natural Science Foundation,China(No.ZR2021QE122)+1 种基金Shandong Provincial Department of Science and Technology Key Project,China(No.2023TZXD021)Shandong Provincial Department of Science and Technology,China(No.ZTYJY-KY-2033-11).
文摘Gold ores in the Jiaozhou region of China are characterized by their abundant reserves,low grade,fine dissemination,and chal-lenges in upgrading.Froth flotation,with xanthate as the collector,is a commonly employed method for enriching auriferous pyrite from these ores.This study aimed to develop a more efficient flotation process by utilizing cavitation nanobubbles for a low-grade gold ore.Batch flotation tests demonstrated that nanobubbles significantly enhanced the flotation performance of auriferous pyrite,as evidenced by improved concentrate S and Au grades and their recoveries.The mechanisms underlying this enhancement were explored by investigat-ing surface nanobubble(SNB)formation,bulk nanobubble(BNB)attachment to hydrophobic pyrite surfaces,and nanobubble-induced agglomeration using atomic force microscopy(AFM)and focused beam reflectance measurement(FBRM).The results revealed that nan-obubble coverage on the pyrite surface is a critical factor influencing surface hydrophobicity and agglomeration.SNBs exhibited higher coverage on pyrite surfaces with increased surface hydrophobicity,flow rate,and cavitation time.Similarly,BNB attachment on pyrite surfaces was significantly increased with surface hydrophobicity and cavitation time.Enhanced surface hydrophobicity,along with higher flow rates and cavitation times,promoted pyrite particle agglomeration owing to the increased nanobubble coverage,ultimately leading to improved flotation performance.
基金supported by the Ordos City Science and Technology Major Project(2021ZD14-16)the National Key Research and Development Program(2018YFC1802904)the Discipline Signature Achievements of the Shanghai Polytechnic University(A10GY23G004-14).
文摘One of the major challenges in the application of microbially induced carbonate precipitation(MICP)is achieving"bacteria freedom",as it necessitates a substantial volume of bacterial solutions.Nevertheless,both insitu bacterial cultivation and transportation of bacterial solutions have proven to be inefficient.In this study,we suggested the utilization of bacteria in the form of dry powder,enabling easy on-site activation and achieving a relatively high urease activity.We conducted MICP curing experiments on gold mine tailings(GMT)using steel slag(SS)as an additive.The results showed that the average unconfined compressive strength(UCS)values of the tailings treated with MICP and MICP+SS reached 0.51 and 0.71 MPa,respectively.In addition,the average leaching reduction rates of Cu,Pb,Cr,Zn,and T-CN in GMT after MICP treatment reached 98.54%,100%,70.94%,59.25%,and 98.02%,respectively,and the average reduction rates after MICP+SS treatment reached 98.77%,100%,88.03%,72.59%,and 98.63%,respectively.SEM,XRD,FT-IR analyses,and ultra-deep field microscopy results confirmed that the MICP treatment produced calcite-based calcium carbonate that filled the inter-tailing pores and cemented them together,and the hydration mechanism was the main reason for the increased curing efficiency of SS.Our research findings demonstrate that bacterial powder can efficiently achieve the objectives of heavy metal removal and tailing solidification.This approach can substantially de-crease the expenses associated with bacterial cultivation and solution transportation,thereby playing a crucial role in advancing the practical implementation of MICP.
基金supported by the Natural Science Foundation of Guangxi(No.2022GXNSFGA035003)the National Natural Science Foundation of China(No.22077021).
文摘To treat cancer and inhibit its metastasis to the greatest extent,we proposed to develop an Au(III)agent to induce immunogenic cell death(ICD)and establish long-term immunity.To this end,we optimized a series of Au(III)2-benzoylpyridine thiosemicarbazone complexes to obtain an Au(III)agent(5b)with excellent cytotoxicity to cancer.The results show that 5b effectively inhibits tumor growth and its metastasis in vivo.Interestingly,we revealed a new mechanism of 5b inhibiting tumor growth and metastasis:5b releases ICD-related damage-associated molecular patterns(DAMPs),such as calreticulin(CRT),ATP and high mobility group box 1(HMGB1)by inducing endoplasmic reticulum stress(ERS)and mitochondrial dysfunction,which then stimulated an antitumor CD8^(+)T cell response and Foxp^(3+)T cell depletion,thus establishing long-action antitumor immunity.
基金support from the National Key Research and Development Program of China(2021YFB3201400,2021YFB3201401,2020YFC1908602)the National Natural Science Foundation of China(21904001 and 61774159)+1 种基金the Anhui Provincial Natural Science Foundation(2008085QF288)the Scientific Research Foundation for the Returned Overseas Chinese Scholars,Anhui Province(2020LCX032).
文摘Metal-organic framework(MOF)nanostructures have emerged as a prominent class of materials in the advancement of electrochemical sensors.The rational design of bimetallic MOF-functionalized microelectrode is of importance for improv-ing the electrochemical performance but still in great challenge.In this work,the bimetallic FeCo-MOF nanostructures were assembled onto a gold disk ultramicroelectrode(Au UME,5.2μm in diameter)via an in-situ electrodeposition method,which enhanced the sensitive detection of epinephrine(EP).The in-situ electrodeposited FeCo-MOF exhibited a character-istic nanoflower-like morphology and was uniformly dispersed on the Au UME.The FeCo-MOF/Au UME demonstrated excellent electrochemical performance on the detection of EP with a high sensitivity of 36.93μA·μmol^(-1)·L·cm^(-2)and a low detection limit of 1.28μmol·L^(-1).It can be attributed to the nonlinear diffusion of EP onto the ultra-micro working substrate,coupled with synergistical catalytic activity of the bimetallic Fe,Co within MOF structure.Furthermore,the FeCo-MOF/Au UME has been successful applied to the analysis of EP in human serum samples,yielding high recovery rates.These results not only contribute to the expansion of the research area of electrochemical sensors,but also provide novel insights and directions into the development of high-performance MOF-based electrochemical sensors.
基金supported by the Young Talent Innovation Team Support Project from Zhengzhou University(No.32213280)the scientific research program of innovation platform in State Tobacco Monopoly Administrationthe State Key Program of National Natural Science Foundation of China(Grant No.32130083).
文摘Dopamine(DA)is a vital neurotransmitter,and accurate detection of its concentration is critical for both clinical diagnos-tics and neuroscience research.Due to its electrochemical activity,DA is commonly detected using electrochemical methods,which are favored for their simplicity,fast response time,and suitability for in vivo analysis.In this work,a highly sensitive DA electrochemical sensor was developed using an Au@MoS_(2)composite,created by modifying molybdenum disulfide(MoS_(2))nanosheets with gold nanoparticles through HAuCl_(4) reduction,and it was aimed at enhancing DA adsorption and improving detection performance.Scanning Electron Microscopy(SEM),transmission electron microscopy(TEM),Energy Dispersive Spectroscopy(EDS),X-ray photoelectron spectroscopy(XPS)and X-ray Diffraction(XRD)confirmed the suc-cessful synthesis of Au@MoS_(2)and the uniform distribution of gold nanoparticles across the MoS_(2)nanosheets.Then,the electrochemical characterization demonstrated that the Au@MoS_(2)/GCE exhibited distinct oxidation peaks in a 10μmol·L^(-1)DA solution,with significantly enhanced electrochemical activity compared to both unmodified GCE and pristine MoS_(2).Furthermore,differential pulse voltammetry(DPV)further revealed a strong linear relationship between DA concentration and the current response in the range of 800 nmol·L^(-1)to 10μmol·L^(-1),with a low detection limit(LOD)of 78.9 nmol·L^(-1)(S/N=3).Additionally,the sensor showed excellent selectivity against other interfering substances.Moreover,the laser-induced Au@MoS_(2)(LIAu@MoS_(2)),with its abundance of negatively charged surface defects,enabled the ultra-sensitive detection of the ultra-low concentrations of DA.In conclusion,the successfully fabricated Au@MoS_(2)based sensor offers advantages such as low cost,ease of operation,and scalability,making it a promising candidate for biosensing applications due to its enhanced DA detection capabilities.
文摘A new gold self-relay catalytic annulation/nucleophilic substitution cascade of 1,3-enyne acetates with cyclic ether acetals is reported,enabling highly diastereoselective access to cyclic etherified cyclopentenones with cyclic quaternary centers in moderate to good yields and>19∶1 dr.This catalysis enables the direct construction of two types of carboncyclic skeletons by adjusting the olefin types of 1,3-enyne acetates.When 1,3-enyne acetates bearing a cyclic alkene unit were used,5~6 fused bicarbocyclic products were diastereoselectively synthesized,whereas the reaction of acyclic 1,3-enyne acetates resulted in five-memebered carbocyclic framework.Notably,cyclic ether acetals are commonly used as protecting groups in traditional multistep organic syntheses,and in this reaction,such reagents serve as electrophilic cyclic ether precursors,achieving new uses for old reagents.The current method demonstrates good functional group compatibility,a broad substrate scope and high diastereoselectivity,providing a new synthetic strategy toward functionalized cyclopentenones.
