Inoculated fermentation enables rapid fermentation of aquatic products.No studies have been conducted on the lipid profiles of inoculated fermented golden pompano(Trachinotus ovatus).In this study,a lipase-producing B...Inoculated fermentation enables rapid fermentation of aquatic products.No studies have been conducted on the lipid profiles of inoculated fermented golden pompano(Trachinotus ovatus).In this study,a lipase-producing Bacillus subtilis with salt tolerance was screened from traditionally fermented golden pompano(TF)and used as a starter culture.Whole-genome sequencing analysis revealed it carries 4 clustered regularly interspaced short vpalindromic repeats structures and 2 genes encoding triacylglycerol lipase.Untargeted lipidomics identified lipid molecules(833)in 6 major classes from B.subtilis SCSMX-2 fermented golden pompano(IF).A total of 28 lipid molecules were upregulated in IF,including phosphatidylcholines(PCs),triacylglycerols(TAGs),and lysophosphatidylcholine.B.subtilis supplementation enhanced the production of polyenyl PCs and mediumand long-chain TAGs.The IF rich in linoleic,docosahexaenoic acids(DHA),and eicosapentaenoic acid were primarily distributed in the sn-2 position of DHA,PC and phosphatidylethanolamine.This research revealed the lipid profiles of IF,providing theoretical basis for the application of B.subtilis in the fermented fish industry.展开更多
"Look at this!This one grows well and big.And its shape,so round and plump.How.adorable,"exclaimed Pema Chosphel as he carefully unearthed a palm-sized gastrodia from the damp soil.Overjoyed,he called his fa..."Look at this!This one grows well and big.And its shape,so round and plump.How.adorable,"exclaimed Pema Chosphel as he carefully unearthed a palm-sized gastrodia from the damp soil.Overjoyed,he called his father to share his excitement.展开更多
Nujiang farmers use the innovative dryland rice cultivation technology developed by a Chinese expert team to grow yields and income.As April ushers in spring,mist clings to the deep valleys of the Nujiang Grand Canyon...Nujiang farmers use the innovative dryland rice cultivation technology developed by a Chinese expert team to grow yields and income.As April ushers in spring,mist clings to the deep valleys of the Nujiang Grand Canyon in Southwest China’s Yunnan Province.High on a mountainside in Ziji Village,Daxingdi Town,Lushui City,Nujiang Lisu Autonomous Prefecture,at an altitude exceeding 1,300 metres,62-year-old Lisu villager Cha Wenxing is planting rice.These demonstration plots,cultivating high-quality upland rice,seem to float amongst the clouds.展开更多
This year marks the 50th anniversary of China-Thailand diplomatic relations as well as the“Golden Jubilee of China-Thailand Friendship.”From February 5 to 8,Thai Prime Minister Paetongtarn Shinawatra made an officia...This year marks the 50th anniversary of China-Thailand diplomatic relations as well as the“Golden Jubilee of China-Thailand Friendship.”From February 5 to 8,Thai Prime Minister Paetongtarn Shinawatra made an official visit to China.On February 8,the two sides issued the Joint Statement between the Government of the Kingdom of Thailand and the Government of the People’s Republic of China on Advancing the Comprehensive Strategic Cooperative Partnership and Building a Thailand-China Community with a Shared Future for Enhanced Stability,Prosperity,and Sustainability through a Forwardlooking and People-Centred Vision.展开更多
As part of events marking the 50th anniversary of diplomatic ties between China and Thailand,a forum themed“Our Golden Friendship:Joining Hands to Build a China-Thailand Community with a Shared Future”was held in Ba...As part of events marking the 50th anniversary of diplomatic ties between China and Thailand,a forum themed“Our Golden Friendship:Joining Hands to Build a China-Thailand Community with a Shared Future”was held in Bangkok on January 17,aiming to deepen mutual trust and enhance exchange between Chinese and Thai media and think tanks.展开更多
This year marks the 50th anniversary of the establishment of China-Thailand diplomatic relations and the“Golden Jubilee of China-Thailand Friendship.”Over the decades,fruitful results have been achieved in bilateral...This year marks the 50th anniversary of the establishment of China-Thailand diplomatic relations and the“Golden Jubilee of China-Thailand Friendship.”Over the decades,fruitful results have been achieved in bilateral cooperation in fields such as politics,economics,security,and cultural exchange.So,what are the key factors contributing to the enduring friendship and the remarkable achievements in bilateral cooperation?And,how does strengthened comprehensive strategic partnership and joint construction of a more stable,prosperous,and sustainable China-Thailand community with a shared future benefit both countries,the region,and even the world?展开更多
To address the problem of high lifespan loss and poor state of charge(SOC)balance of electric vehicles(EVs)participating in grid peak shaving,an improved golden eagle optimizer(IGEO)algorithm for EV grouping control s...To address the problem of high lifespan loss and poor state of charge(SOC)balance of electric vehicles(EVs)participating in grid peak shaving,an improved golden eagle optimizer(IGEO)algorithm for EV grouping control strategy is proposed for peak shaving sce-narios.First,considering the difference between peak and valley loads and the operating costs of EVs,a peak shaving model for EVs is constructed.Second,the design of IGEO has improved the global exploration and local development capabilities of the golden eagle optimizer(GEO)algorithm.Subsequently,IGEO is used to solve the peak shaving model and obtain the overall EV grid connected charging and discharging instructions.Next,using the k-means algorithm,EVs are dynamically divided into priority charging groups,backup groups,and priority discharging groups based on SOC differences.Finally,a dual layer power distribution scheme for EVs is designed.The upper layer determines the charging and discharging sequences and instructions for the three groups of EVs,whereas the lower layer allocates the charging and discharging instructions for each group to each EV.The proposed strategy was simulated and verified,and the results showed that the designed IGEO had faster optimization speed and higher optimization accuracy.The pro-posed EV grouping control strategy effectively reduces the peak-valley difference in the power grid,reduces the operational life loss of EVs,and maintains a better SOC balance for EVs.展开更多
Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate asse...Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate assembly technique,utilizing Escherichia coli as the model organism.Through polymerase chain reaction(PCR)amplification,restriction enzyme digestion,ligation,transformation,and Sanger sequencing,we assessed the operational efficiency and cloning fidelity of both strategies.Our results demonstrated that Golden Gate assembly,leveraging type IIS restriction enzymes and simultaneous ligation,significantly enhanced cloning efficiency and precision,particularly for seamless multi-fragment assembly.In contrast,the classical cloning approach maintained certain advantages in simplicity and robustness for specific experimental conditions.Challenges encountered during transformation and sequencing highlighted the critical impact of technical accuracy on experimental outcomes.This study underscores the importance of selecting appropriate cloning methodologies tailored to experimental objectives and laboratory capabilities,providing a foundation for optimized molecular cloning workflows in future synthetic biology and biotechnology applications.展开更多
In this paper,the necessity of constructing a golden course for Advanced Mathematics is presented.For teachers,golden courses enhance their teaching ability and job satisfaction.For students,golden courses improve the...In this paper,the necessity of constructing a golden course for Advanced Mathematics is presented.For teachers,golden courses enhance their teaching ability and job satisfaction.For students,golden courses improve their sense of learning value and significance and encourage them to actively learn and participate deeply.Next,the relevant content of course ideological and political construction is provided.Finally,a specific approach was proposed to create a golden course for Advanced Mathematics by integrating ideological and political education into the curriculum.展开更多
Reliable Cluster Head(CH)selectionbased routing protocols are necessary for increasing the packet transmission efficiency with optimal path discovery that never introduces degradation over the transmission reliability...Reliable Cluster Head(CH)selectionbased routing protocols are necessary for increasing the packet transmission efficiency with optimal path discovery that never introduces degradation over the transmission reliability.In this paper,Hybrid Golden Jackal,and Improved Whale Optimization Algorithm(HGJIWOA)is proposed as an effective and optimal routing protocol that guarantees efficient routing of data packets in the established between the CHs and the movable sink.This HGJIWOA included the phases of Dynamic Lens-Imaging Learning Strategy and Novel Update Rules for determining the reliable route essential for data packets broadcasting attained through fitness measure estimation-based CH selection.The process of CH selection achieved using Golden Jackal Optimization Algorithm(GJOA)completely depends on the factors of maintainability,consistency,trust,delay,and energy.The adopted GJOA algorithm play a dominant role in determining the optimal path of routing depending on the parameter of reduced delay and minimal distance.It further utilized Improved Whale Optimisation Algorithm(IWOA)for forwarding the data from chosen CHs to the BS via optimized route depending on the parameters of energy and distance.It also included a reliable route maintenance process that aids in deciding the selected route through which data need to be transmitted or re-routed.The simulation outcomes of the proposed HGJIWOA mechanism with different sensor nodes confirmed an improved mean throughput of 18.21%,sustained residual energy of 19.64%with minimized end-to-end delay of 21.82%,better than the competitive CH selection approaches.展开更多
Popular fermented golden pomfret(Trachinotus ovatus)is prepared via spontaneous fermentation;however,the mechanisms underlying the regulation of its flavor development remain unclear.This study shows the roles of the ...Popular fermented golden pomfret(Trachinotus ovatus)is prepared via spontaneous fermentation;however,the mechanisms underlying the regulation of its flavor development remain unclear.This study shows the roles of the complex microbiota and the dynamic changes in microbial community and flavor compounds during fish fermentation.Single-molecule real-time sequencing and molecular networking analysis revealed the correlations among different microbial genera and the relationships between microbial taxa and volatile compounds.Mechanisms underlying flavor development were also elucidated via KEGG based functional annotations.Clostridium,Shewanella,and Staphylococcus were the dominant microbial genera.Forty-nine volatile compounds were detected in the fermented fish samples,with thirteen identified as characteristic volatile compounds(ROAV>1).Volatile profiles resulted from the interactions among the microorganisms and derived enzymes,with the main metabolic pathways being amino acid biosynthesis/metabolism,carbon metabolism,and glycolysis/gluconeogenesis.This study demonstrated the approaches for distinguishing key microbiota associated with volatile compounds and monitoring the industrial production of high-quality fermented fish products.展开更多
Medical image segmentation is a powerful and evolving technology in medical diagnosis.In fact,it has been identified as a very effective tool to support and accompany doctors in their fight against the spread of the c...Medical image segmentation is a powerful and evolving technology in medical diagnosis.In fact,it has been identified as a very effective tool to support and accompany doctors in their fight against the spread of the coronavirus(COVID-19).Various techniques have been utilized for COVID-19 image segmentation,including Multilevel Thresholding(MLT)-based meta-heuristics,which are considered crucial in addressing this issue.However,despite their importance,meta-heuristics have significant limitations.Specifically,the imbalance between exploration and exploitation,as well as premature convergence,can cause the optimization process to become stuck in local optima,resulting in unsatisfactory segmentation results.In this paper,an enhanced War Strategy Chimp Optimization Algorithm(WSChOA)is proposed to address MLT problems.Two strategies are incorporated into the traditional Chimp Optimization Algorithm.Golden update mechanism that provides diversity in the population.Additionally,the attack and defense strategies are incorporated to improve the search space leading to avoiding local optima.The experimental results were conducted by comparing WSChoA with recent and well-known algorithms using various evaluation metrics such as Feature Similarity Index(FSIM),Structural Similarity Index(SSIM),Peak signal-to-Noise Ratio(PSNR),Standard deviation(STD),Freidman Test(FT),and Wilcoxon Sign Rank Test(WSRT).The results obtained by WSChoA surpassed those of other optimization techniques in terms of robustness and accuracy,indicating that it is a powerful tool for image segmentation.展开更多
This article primarily examines the current state of tax collection and management,alongside other associated issues.It integrates insights from China’s“14th Five-Year Plan”and anticipates the imminent implementati...This article primarily examines the current state of tax collection and management,alongside other associated issues.It integrates insights from China’s“14th Five-Year Plan”and anticipates the imminent implementation of the“Golden Tax Phase Four.”With this backdrop,the article offers recommendations for advancing the reform of the tax collection and management system,fostering the development of intelligent taxation,and accelerating the modernization of tax collection and management in China.展开更多
Dynamic Economic Emission Dispatch(DEED)aims to optimize control over fuel cost and pollution emission,two conflicting objectives,by scheduling the output power of various units at specific times.Although many methods...Dynamic Economic Emission Dispatch(DEED)aims to optimize control over fuel cost and pollution emission,two conflicting objectives,by scheduling the output power of various units at specific times.Although many methods well-performed on the DEED problem,most of them fail to achieve expected results in practice due to a lack of effective trade-off mechanisms between the convergence and diversity of non-dominated optimal dispatching solutions.To address this issue,a new multi-objective solver called Multi-Objective Golden Jackal Optimization(MOGJO)algorithm is proposed to cope with the DEED problem.The proposed algorithm first stores non-dominated optimal solutions found so far into an archive.Then,it chooses the best dispatching solution from the archive as the leader through a selection mechanism designed based on elite selection strategy and Euclidean distance index method.This mechanism can guide the algorithm to search for better dispatching solutions in the direction of reducing fuel costs and pollutant emissions.Moreover,the basic golden jackal optimization algorithm has the drawback of insufficient search,which hinders its ability to effectively discover more Pareto solutions.To this end,a non-linear control parameter based on the cosine function is introduced to enhance global exploration of the dispatching space,thus improving the efficiency of finding the optimal dispatching solutions.The proposed MOGJO is evaluated on the latest CEC benchmark test functions,and its superiority over the state-of-the-art multi-objective optimizers is highlighted by performance indicators.Also,empirical results on 5-unit,10-unit,IEEE 30-bus,and 30-unit systems show that the MOGJO can provide competitive compromise scheduling solutions compared to published DEED methods.Finally,in the analysis of the Pareto dominance relationship and the Euclidean distance index,the optimal dispatching solutions provided by MOGJO are the closest to the ideal solutions for minimizing fuel costs and pollution emissions simultaneously,compared to the latest published DEED solutions.展开更多
This study sets up two new merit functions,which are minimized for the detection of real eigenvalue and complex eigenvalue to address nonlinear eigenvalue problems.For each eigen-parameter the vector variable is solve...This study sets up two new merit functions,which are minimized for the detection of real eigenvalue and complex eigenvalue to address nonlinear eigenvalue problems.For each eigen-parameter the vector variable is solved from a nonhomogeneous linear system obtained by reducing the number of eigen-equation one less,where one of the nonzero components of the eigenvector is normalized to the unit and moves the column containing that component to the right-hand side as a nonzero input vector.1D and 2D golden section search algorithms are employed to minimize the merit functions to locate real and complex eigenvalues.Simultaneously,the real and complex eigenvectors can be computed very accurately.A simpler approach to the nonlinear eigenvalue problems is proposed,which implements a normalization condition for the uniqueness of the eigenvector into the eigenequation directly.The real eigenvalues can be computed by the fictitious time integration method(FTIM),which saves computational costs compared to the one-dimensional golden section search algorithm(1D GSSA).The simpler method is also combined with the Newton iterationmethod,which is convergent very fast.All the proposed methods are easily programmed to compute the eigenvalue and eigenvector with high accuracy and efficiency.展开更多
[Objectives]The paper was to evaluate the effectiveness of Xiulijing in the management of rust in Golden Delicious apple cultivation.[Methods]The Golden Delicious apple was utilized as the test material.During the you...[Objectives]The paper was to evaluate the effectiveness of Xiulijing in the management of rust in Golden Delicious apple cultivation.[Methods]The Golden Delicious apple was utilized as the test material.During the young fruit stage,various dilutions of Xiulijing were applied,specifically at concentrations of 200,400,600,800,1000,and 2000 times.Additionally,both the application of a 3000-fold dilution of GA 4+7 and the practice of fruit bagging were designated as the control treatments,while a water spray was utilized as the blank control.The fruit rust index and the incidence of rust-infected fruits were evaluated to assess the effectiveness of control measures for fruit rust in Golden Delicious apple cultivation.[Results]The application of Xiulijing,with dilutions ranging from 200 to 2000 times,exhibited a significant impact on reducing both the fruit rust index and the incidence of rust-infected fruits in Golden Delicious apples.The application of a 1000-fold dilution of Xiulijing resulted in a statistically significant reduction in both the fruit rust index and the incidence of rust-infected fruits,with reduction rates of 21.1%and 31.5%,respectively.Among the various strategies for the prevention and control of apple rust,fruit bagging emerged as the most effective method.The other two technical measures demonstrated significant control effects;however,no substantial differences were observed between them.[Conclusions]The biological control agent Xiulijing is more deserving of promotion and application in practical production due to its significantly superior economic benefits,safety,and stability.展开更多
In cosmetic surgery, golden threads have been used in thread lift procedures, wherein golden threads are placed under the skin of the neck and chin. These are mainly applied in the maxillofacial region adjacent to the...In cosmetic surgery, golden threads have been used in thread lift procedures, wherein golden threads are placed under the skin of the neck and chin. These are mainly applied in the maxillofacial region adjacent to the sites of dental treatment to achieve cosmetic benefits. However, as dentists typically lack sufficient knowledge about the golden thread lift procedure, it may present a challenge to dental examinations and treatments. It is therefore crucial for dentists to have a comprehensive understanding of the procedure. This case report covers our experience with the dental examination of a patient with golden threads. We emphasize the dental complications and precautions that should be taken for such cases. These golden threads are made of pure gold, nonabsorbable, and can be broken. These can obstruct dental examination, as well as cause metal allergies and foreign body granulomas. Additionally, it is difficult to completely remove these threads when they break apart. In the future, since more patients are expected to undergo this procedure, it is possible to encounter these golden threads as artifacts on imaging. Therefore, it is important to educate dentists about the golden thread lift procedure and its dental implications. It is also imperative to determine whether the patient has undergone the golden thread lift procedure in the medical questionnaire prior to the magnetic resonance imaging examination. Thus, dentists should be able to conduct a detailed interview with the patient, determine the feasibility of examination or treatment, and communicate this assessment to the patient.展开更多
As we approach the golden jubilee of Malaysia-China diplomatic relations in May 2024,it is both timely and essential to reflect on the journey that has shaped one of the most dynamic and influential economic partnersh...As we approach the golden jubilee of Malaysia-China diplomatic relations in May 2024,it is both timely and essential to reflect on the journey that has shaped one of the most dynamic and influential economic partnerships in Asia.This 50-year saga is not merely a story of diplomatic ties;it is a testament to enduring friendship,strategic cooperation and mutual growth that have significantly impacted both nations and the wider region.The relationship between Malaysia and China,which formally began in 1974,was groundbreaking in many respects.It symbolized not only Malaysia’s commitment to a forward-looking foreign policy but also China’s expanding engagement with Southeast Asia.Over the decades,this relationship has evolved from tentative initial contact into a robust,multifaceted partnership.展开更多
Wuzhen Theatre Festival,had its 11th edition held,took root from the soil of the Chinese drama,with a variety of dramatic forms sprouting as the curtain rising and flling.From October 17 to October 27.the 11th Wuzhen ...Wuzhen Theatre Festival,had its 11th edition held,took root from the soil of the Chinese drama,with a variety of dramatic forms sprouting as the curtain rising and flling.From October 17 to October 27.the 11th Wuzhen Theatre Festival with the theme of"Solidity"was held in Wuzhen,Tongxiang City,Zheliang Province.展开更多
基金supported by the National Natural Science Foundation of China(32372367)the earmarked fund for CARS(CARS-47)+1 种基金Natural Science Foundation of Hainan Provine(323CXTD391)the Special Scientific Research Funds for Central Non-profit Institutes and the Chinese Academy of Fishery Sciences(2020TD69)。
文摘Inoculated fermentation enables rapid fermentation of aquatic products.No studies have been conducted on the lipid profiles of inoculated fermented golden pompano(Trachinotus ovatus).In this study,a lipase-producing Bacillus subtilis with salt tolerance was screened from traditionally fermented golden pompano(TF)and used as a starter culture.Whole-genome sequencing analysis revealed it carries 4 clustered regularly interspaced short vpalindromic repeats structures and 2 genes encoding triacylglycerol lipase.Untargeted lipidomics identified lipid molecules(833)in 6 major classes from B.subtilis SCSMX-2 fermented golden pompano(IF).A total of 28 lipid molecules were upregulated in IF,including phosphatidylcholines(PCs),triacylglycerols(TAGs),and lysophosphatidylcholine.B.subtilis supplementation enhanced the production of polyenyl PCs and mediumand long-chain TAGs.The IF rich in linoleic,docosahexaenoic acids(DHA),and eicosapentaenoic acid were primarily distributed in the sn-2 position of DHA,PC and phosphatidylethanolamine.This research revealed the lipid profiles of IF,providing theoretical basis for the application of B.subtilis in the fermented fish industry.
文摘"Look at this!This one grows well and big.And its shape,so round and plump.How.adorable,"exclaimed Pema Chosphel as he carefully unearthed a palm-sized gastrodia from the damp soil.Overjoyed,he called his father to share his excitement.
文摘Nujiang farmers use the innovative dryland rice cultivation technology developed by a Chinese expert team to grow yields and income.As April ushers in spring,mist clings to the deep valleys of the Nujiang Grand Canyon in Southwest China’s Yunnan Province.High on a mountainside in Ziji Village,Daxingdi Town,Lushui City,Nujiang Lisu Autonomous Prefecture,at an altitude exceeding 1,300 metres,62-year-old Lisu villager Cha Wenxing is planting rice.These demonstration plots,cultivating high-quality upland rice,seem to float amongst the clouds.
文摘This year marks the 50th anniversary of China-Thailand diplomatic relations as well as the“Golden Jubilee of China-Thailand Friendship.”From February 5 to 8,Thai Prime Minister Paetongtarn Shinawatra made an official visit to China.On February 8,the two sides issued the Joint Statement between the Government of the Kingdom of Thailand and the Government of the People’s Republic of China on Advancing the Comprehensive Strategic Cooperative Partnership and Building a Thailand-China Community with a Shared Future for Enhanced Stability,Prosperity,and Sustainability through a Forwardlooking and People-Centred Vision.
文摘As part of events marking the 50th anniversary of diplomatic ties between China and Thailand,a forum themed“Our Golden Friendship:Joining Hands to Build a China-Thailand Community with a Shared Future”was held in Bangkok on January 17,aiming to deepen mutual trust and enhance exchange between Chinese and Thai media and think tanks.
文摘This year marks the 50th anniversary of the establishment of China-Thailand diplomatic relations and the“Golden Jubilee of China-Thailand Friendship.”Over the decades,fruitful results have been achieved in bilateral cooperation in fields such as politics,economics,security,and cultural exchange.So,what are the key factors contributing to the enduring friendship and the remarkable achievements in bilateral cooperation?And,how does strengthened comprehensive strategic partnership and joint construction of a more stable,prosperous,and sustainable China-Thailand community with a shared future benefit both countries,the region,and even the world?
基金supported by the National Natural Science Foundation of China(52077078)China Southern Power Grid Company Limited 036000KK52220004(GDKJXM20220147).
文摘To address the problem of high lifespan loss and poor state of charge(SOC)balance of electric vehicles(EVs)participating in grid peak shaving,an improved golden eagle optimizer(IGEO)algorithm for EV grouping control strategy is proposed for peak shaving sce-narios.First,considering the difference between peak and valley loads and the operating costs of EVs,a peak shaving model for EVs is constructed.Second,the design of IGEO has improved the global exploration and local development capabilities of the golden eagle optimizer(GEO)algorithm.Subsequently,IGEO is used to solve the peak shaving model and obtain the overall EV grid connected charging and discharging instructions.Next,using the k-means algorithm,EVs are dynamically divided into priority charging groups,backup groups,and priority discharging groups based on SOC differences.Finally,a dual layer power distribution scheme for EVs is designed.The upper layer determines the charging and discharging sequences and instructions for the three groups of EVs,whereas the lower layer allocates the charging and discharging instructions for each group to each EV.The proposed strategy was simulated and verified,and the results showed that the designed IGEO had faster optimization speed and higher optimization accuracy.The pro-posed EV grouping control strategy effectively reduces the peak-valley difference in the power grid,reduces the operational life loss of EVs,and maintains a better SOC balance for EVs.
文摘Molecular cloning remains a cornerstone technique in genetic engineering and synthetic biology.In this study,we conducted a systematic comparative analysis between the classical cloning method and the Golden Gate assembly technique,utilizing Escherichia coli as the model organism.Through polymerase chain reaction(PCR)amplification,restriction enzyme digestion,ligation,transformation,and Sanger sequencing,we assessed the operational efficiency and cloning fidelity of both strategies.Our results demonstrated that Golden Gate assembly,leveraging type IIS restriction enzymes and simultaneous ligation,significantly enhanced cloning efficiency and precision,particularly for seamless multi-fragment assembly.In contrast,the classical cloning approach maintained certain advantages in simplicity and robustness for specific experimental conditions.Challenges encountered during transformation and sequencing highlighted the critical impact of technical accuracy on experimental outcomes.This study underscores the importance of selecting appropriate cloning methodologies tailored to experimental objectives and laboratory capabilities,providing a foundation for optimized molecular cloning workflows in future synthetic biology and biotechnology applications.
基金Project of Heilongjiang University of Finance and Economics(XJYB2024056)Project of Heilongjiang University of Finance and Economics(XJYB2024057)。
文摘In this paper,the necessity of constructing a golden course for Advanced Mathematics is presented.For teachers,golden courses enhance their teaching ability and job satisfaction.For students,golden courses improve their sense of learning value and significance and encourage them to actively learn and participate deeply.Next,the relevant content of course ideological and political construction is provided.Finally,a specific approach was proposed to create a golden course for Advanced Mathematics by integrating ideological and political education into the curriculum.
文摘Reliable Cluster Head(CH)selectionbased routing protocols are necessary for increasing the packet transmission efficiency with optimal path discovery that never introduces degradation over the transmission reliability.In this paper,Hybrid Golden Jackal,and Improved Whale Optimization Algorithm(HGJIWOA)is proposed as an effective and optimal routing protocol that guarantees efficient routing of data packets in the established between the CHs and the movable sink.This HGJIWOA included the phases of Dynamic Lens-Imaging Learning Strategy and Novel Update Rules for determining the reliable route essential for data packets broadcasting attained through fitness measure estimation-based CH selection.The process of CH selection achieved using Golden Jackal Optimization Algorithm(GJOA)completely depends on the factors of maintainability,consistency,trust,delay,and energy.The adopted GJOA algorithm play a dominant role in determining the optimal path of routing depending on the parameter of reduced delay and minimal distance.It further utilized Improved Whale Optimisation Algorithm(IWOA)for forwarding the data from chosen CHs to the BS via optimized route depending on the parameters of energy and distance.It also included a reliable route maintenance process that aids in deciding the selected route through which data need to be transmitted or re-routed.The simulation outcomes of the proposed HGJIWOA mechanism with different sensor nodes confirmed an improved mean throughput of 18.21%,sustained residual energy of 19.64%with minimized end-to-end delay of 21.82%,better than the competitive CH selection approaches.
基金supported by the National Natural Science Foundation of China(32001733)the Earmarked fund for CARS(CARS-47)+3 种基金Guangxi Natural Science Foundation Program(2021GXNSFAA196023)Guangdong Basic and Applied Basic Research Foundation(2021A1515010833)Young Talent Support Project of Guangzhou Association for Science and Technology(QT20220101142)the Special Scientific Research Funds for Central Non-profit Institutes,Chinese Academy of Fishery Sciences(2020TD69)。
文摘Popular fermented golden pomfret(Trachinotus ovatus)is prepared via spontaneous fermentation;however,the mechanisms underlying the regulation of its flavor development remain unclear.This study shows the roles of the complex microbiota and the dynamic changes in microbial community and flavor compounds during fish fermentation.Single-molecule real-time sequencing and molecular networking analysis revealed the correlations among different microbial genera and the relationships between microbial taxa and volatile compounds.Mechanisms underlying flavor development were also elucidated via KEGG based functional annotations.Clostridium,Shewanella,and Staphylococcus were the dominant microbial genera.Forty-nine volatile compounds were detected in the fermented fish samples,with thirteen identified as characteristic volatile compounds(ROAV>1).Volatile profiles resulted from the interactions among the microorganisms and derived enzymes,with the main metabolic pathways being amino acid biosynthesis/metabolism,carbon metabolism,and glycolysis/gluconeogenesis.This study demonstrated the approaches for distinguishing key microbiota associated with volatile compounds and monitoring the industrial production of high-quality fermented fish products.
文摘Medical image segmentation is a powerful and evolving technology in medical diagnosis.In fact,it has been identified as a very effective tool to support and accompany doctors in their fight against the spread of the coronavirus(COVID-19).Various techniques have been utilized for COVID-19 image segmentation,including Multilevel Thresholding(MLT)-based meta-heuristics,which are considered crucial in addressing this issue.However,despite their importance,meta-heuristics have significant limitations.Specifically,the imbalance between exploration and exploitation,as well as premature convergence,can cause the optimization process to become stuck in local optima,resulting in unsatisfactory segmentation results.In this paper,an enhanced War Strategy Chimp Optimization Algorithm(WSChOA)is proposed to address MLT problems.Two strategies are incorporated into the traditional Chimp Optimization Algorithm.Golden update mechanism that provides diversity in the population.Additionally,the attack and defense strategies are incorporated to improve the search space leading to avoiding local optima.The experimental results were conducted by comparing WSChoA with recent and well-known algorithms using various evaluation metrics such as Feature Similarity Index(FSIM),Structural Similarity Index(SSIM),Peak signal-to-Noise Ratio(PSNR),Standard deviation(STD),Freidman Test(FT),and Wilcoxon Sign Rank Test(WSRT).The results obtained by WSChoA surpassed those of other optimization techniques in terms of robustness and accuracy,indicating that it is a powerful tool for image segmentation.
文摘This article primarily examines the current state of tax collection and management,alongside other associated issues.It integrates insights from China’s“14th Five-Year Plan”and anticipates the imminent implementation of the“Golden Tax Phase Four.”With this backdrop,the article offers recommendations for advancing the reform of the tax collection and management system,fostering the development of intelligent taxation,and accelerating the modernization of tax collection and management in China.
基金supported by the National Natural Science Foundation of China under Grant No.61802328,61972333,and 61771415.
文摘Dynamic Economic Emission Dispatch(DEED)aims to optimize control over fuel cost and pollution emission,two conflicting objectives,by scheduling the output power of various units at specific times.Although many methods well-performed on the DEED problem,most of them fail to achieve expected results in practice due to a lack of effective trade-off mechanisms between the convergence and diversity of non-dominated optimal dispatching solutions.To address this issue,a new multi-objective solver called Multi-Objective Golden Jackal Optimization(MOGJO)algorithm is proposed to cope with the DEED problem.The proposed algorithm first stores non-dominated optimal solutions found so far into an archive.Then,it chooses the best dispatching solution from the archive as the leader through a selection mechanism designed based on elite selection strategy and Euclidean distance index method.This mechanism can guide the algorithm to search for better dispatching solutions in the direction of reducing fuel costs and pollutant emissions.Moreover,the basic golden jackal optimization algorithm has the drawback of insufficient search,which hinders its ability to effectively discover more Pareto solutions.To this end,a non-linear control parameter based on the cosine function is introduced to enhance global exploration of the dispatching space,thus improving the efficiency of finding the optimal dispatching solutions.The proposed MOGJO is evaluated on the latest CEC benchmark test functions,and its superiority over the state-of-the-art multi-objective optimizers is highlighted by performance indicators.Also,empirical results on 5-unit,10-unit,IEEE 30-bus,and 30-unit systems show that the MOGJO can provide competitive compromise scheduling solutions compared to published DEED methods.Finally,in the analysis of the Pareto dominance relationship and the Euclidean distance index,the optimal dispatching solutions provided by MOGJO are the closest to the ideal solutions for minimizing fuel costs and pollution emissions simultaneously,compared to the latest published DEED solutions.
基金the National Science and Tech-nology Council,Taiwan for their financial support(Grant Number NSTC 111-2221-E-019-048).
文摘This study sets up two new merit functions,which are minimized for the detection of real eigenvalue and complex eigenvalue to address nonlinear eigenvalue problems.For each eigen-parameter the vector variable is solved from a nonhomogeneous linear system obtained by reducing the number of eigen-equation one less,where one of the nonzero components of the eigenvector is normalized to the unit and moves the column containing that component to the right-hand side as a nonzero input vector.1D and 2D golden section search algorithms are employed to minimize the merit functions to locate real and complex eigenvalues.Simultaneously,the real and complex eigenvectors can be computed very accurately.A simpler approach to the nonlinear eigenvalue problems is proposed,which implements a normalization condition for the uniqueness of the eigenvector into the eigenequation directly.The real eigenvalues can be computed by the fictitious time integration method(FTIM),which saves computational costs compared to the one-dimensional golden section search algorithm(1D GSSA).The simpler method is also combined with the Newton iterationmethod,which is convergent very fast.All the proposed methods are easily programmed to compute the eigenvalue and eigenvector with high accuracy and efficiency.
文摘[Objectives]The paper was to evaluate the effectiveness of Xiulijing in the management of rust in Golden Delicious apple cultivation.[Methods]The Golden Delicious apple was utilized as the test material.During the young fruit stage,various dilutions of Xiulijing were applied,specifically at concentrations of 200,400,600,800,1000,and 2000 times.Additionally,both the application of a 3000-fold dilution of GA 4+7 and the practice of fruit bagging were designated as the control treatments,while a water spray was utilized as the blank control.The fruit rust index and the incidence of rust-infected fruits were evaluated to assess the effectiveness of control measures for fruit rust in Golden Delicious apple cultivation.[Results]The application of Xiulijing,with dilutions ranging from 200 to 2000 times,exhibited a significant impact on reducing both the fruit rust index and the incidence of rust-infected fruits in Golden Delicious apples.The application of a 1000-fold dilution of Xiulijing resulted in a statistically significant reduction in both the fruit rust index and the incidence of rust-infected fruits,with reduction rates of 21.1%and 31.5%,respectively.Among the various strategies for the prevention and control of apple rust,fruit bagging emerged as the most effective method.The other two technical measures demonstrated significant control effects;however,no substantial differences were observed between them.[Conclusions]The biological control agent Xiulijing is more deserving of promotion and application in practical production due to its significantly superior economic benefits,safety,and stability.
文摘In cosmetic surgery, golden threads have been used in thread lift procedures, wherein golden threads are placed under the skin of the neck and chin. These are mainly applied in the maxillofacial region adjacent to the sites of dental treatment to achieve cosmetic benefits. However, as dentists typically lack sufficient knowledge about the golden thread lift procedure, it may present a challenge to dental examinations and treatments. It is therefore crucial for dentists to have a comprehensive understanding of the procedure. This case report covers our experience with the dental examination of a patient with golden threads. We emphasize the dental complications and precautions that should be taken for such cases. These golden threads are made of pure gold, nonabsorbable, and can be broken. These can obstruct dental examination, as well as cause metal allergies and foreign body granulomas. Additionally, it is difficult to completely remove these threads when they break apart. In the future, since more patients are expected to undergo this procedure, it is possible to encounter these golden threads as artifacts on imaging. Therefore, it is important to educate dentists about the golden thread lift procedure and its dental implications. It is also imperative to determine whether the patient has undergone the golden thread lift procedure in the medical questionnaire prior to the magnetic resonance imaging examination. Thus, dentists should be able to conduct a detailed interview with the patient, determine the feasibility of examination or treatment, and communicate this assessment to the patient.
文摘As we approach the golden jubilee of Malaysia-China diplomatic relations in May 2024,it is both timely and essential to reflect on the journey that has shaped one of the most dynamic and influential economic partnerships in Asia.This 50-year saga is not merely a story of diplomatic ties;it is a testament to enduring friendship,strategic cooperation and mutual growth that have significantly impacted both nations and the wider region.The relationship between Malaysia and China,which formally began in 1974,was groundbreaking in many respects.It symbolized not only Malaysia’s commitment to a forward-looking foreign policy but also China’s expanding engagement with Southeast Asia.Over the decades,this relationship has evolved from tentative initial contact into a robust,multifaceted partnership.
文摘Wuzhen Theatre Festival,had its 11th edition held,took root from the soil of the Chinese drama,with a variety of dramatic forms sprouting as the curtain rising and flling.From October 17 to October 27.the 11th Wuzhen Theatre Festival with the theme of"Solidity"was held in Wuzhen,Tongxiang City,Zheliang Province.
