Amid the escalating plastic pollution issue, the development of biodegradable and recyclable polymeric materials has become a focus within the scientific community. Chain extenders, which are an important class of com...Amid the escalating plastic pollution issue, the development of biodegradable and recyclable polymeric materials has become a focus within the scientific community. Chain extenders, which are an important class of compounds, facilitate the elongation of polymer chains through reactive functional groups, thereby enhancing the performance of the materials. Epoxy-based chain extenders, due to their cost-effectiveness, low toxicity, high reaction efficiency, and effective reactivity with hydroxyl and carboxyl groups, have emerged as a promising class of chain extenders. This manuscript comprehensively elaborates on the varieties, structural characteristics, and performance of chain extenders, the challenges they face, and the methods for their modification. Special emphasis is placed on the application of epoxy-based chain extenders in biodegradable polymers, such as polylactic acid (PLA), and their subsequent influence on the structural and performance properties of these materials.展开更多
自CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)基因组编辑技术发现以来,迅速在作物中得到广泛应用。但是,CRISPR/Cas9多基因编辑系统在大豆中的研究尚待开发。本文利用CRISPR...自CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)基因组编辑技术发现以来,迅速在作物中得到广泛应用。但是,CRISPR/Cas9多基因编辑系统在大豆中的研究尚待开发。本文利用CRISPR/Cas9介导的多基因编辑系统,分别构建了两个载体,一个载体含6个靶点,编辑7个大豆基因(4个Glycine max ASYMMETRIC LEAVES1(GmAS1)同源基因和3个GmAS2同源基因),另一个载体含8个靶点,编辑11个G.max AGAMOUS家族同源基因(4个GmAG同源基因,2个G.max SEEDSTICK(GmSTK)同源基因和5个G.max SHATTERPROOF1(GmSHP1/2)同源基因)。大豆遗传转化后,经表型鉴定和靶点检测发现,CRISPR/Cas9介导的多基因编辑系统在大豆中成功实现了多基因编辑。当3个GmAS1同源基因和3个GmAS2同源基因同时突变时,导致大豆叶片向远轴面弯曲、皱缩且叶柄变短的表型。当2个GmSHP1同源基因和2个GmSTK同源基因同时突变时,导致豆荚停止发育的不育表型。展开更多
文摘Amid the escalating plastic pollution issue, the development of biodegradable and recyclable polymeric materials has become a focus within the scientific community. Chain extenders, which are an important class of compounds, facilitate the elongation of polymer chains through reactive functional groups, thereby enhancing the performance of the materials. Epoxy-based chain extenders, due to their cost-effectiveness, low toxicity, high reaction efficiency, and effective reactivity with hydroxyl and carboxyl groups, have emerged as a promising class of chain extenders. This manuscript comprehensively elaborates on the varieties, structural characteristics, and performance of chain extenders, the challenges they face, and the methods for their modification. Special emphasis is placed on the application of epoxy-based chain extenders in biodegradable polymers, such as polylactic acid (PLA), and their subsequent influence on the structural and performance properties of these materials.
文摘自CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)基因组编辑技术发现以来,迅速在作物中得到广泛应用。但是,CRISPR/Cas9多基因编辑系统在大豆中的研究尚待开发。本文利用CRISPR/Cas9介导的多基因编辑系统,分别构建了两个载体,一个载体含6个靶点,编辑7个大豆基因(4个Glycine max ASYMMETRIC LEAVES1(GmAS1)同源基因和3个GmAS2同源基因),另一个载体含8个靶点,编辑11个G.max AGAMOUS家族同源基因(4个GmAG同源基因,2个G.max SEEDSTICK(GmSTK)同源基因和5个G.max SHATTERPROOF1(GmSHP1/2)同源基因)。大豆遗传转化后,经表型鉴定和靶点检测发现,CRISPR/Cas9介导的多基因编辑系统在大豆中成功实现了多基因编辑。当3个GmAS1同源基因和3个GmAS2同源基因同时突变时,导致大豆叶片向远轴面弯曲、皱缩且叶柄变短的表型。当2个GmSHP1同源基因和2个GmSTK同源基因同时突变时,导致豆荚停止发育的不育表型。
