期刊文献+
共找到3篇文章
< 1 >
每页显示 20 50 100
大豆GmAMS基因及其启动子的克隆和表达分析 被引量:3
1
作者 金玲 张浩 +3 位作者 王松明 李强 丁先龙 杨守萍 《大豆科学》 CAS CSCD 北大核心 2019年第6期889-897,共9页
bHLH(basic Helix-Loop-Helix)转录因子在植物雄配子发育中具有重要的调控作用。为探究bHLH转录因子在大豆花发育中的作用,以大豆细胞质雄性不育系NJCMS5A的花芽cDNA和叶片DNA为模板,通过RT-PCR克隆得到具有典型bHLH结构域的GmAMS基因... bHLH(basic Helix-Loop-Helix)转录因子在植物雄配子发育中具有重要的调控作用。为探究bHLH转录因子在大豆花发育中的作用,以大豆细胞质雄性不育系NJCMS5A的花芽cDNA和叶片DNA为模板,通过RT-PCR克隆得到具有典型bHLH结构域的GmAMS基因及其启动子区域,并进行生物信息学分析和时空表达分析。生物信息学分析结果显示GmAMS基因的编码区序列全长为1 716 bp,编码571个氨基酸。亚细胞定位预测GmAMS位于细胞核中。荧光定量分析结果显示,相对于保持系NJCMS5B,在不育系NJCMS5A的花芽中GmAMS基因的表达水平显著下调。组织化学染色结果表明GmAMS启动子驱动的GUS蛋白主要集中在转基因拟南芥的幼小花药中表达。研究结果为进一步探究GmAMS在大豆花发育中的生物学功能和调控机制奠定了基础。 展开更多
关键词 大豆 gmamS基因 启动子 基因克隆 表达分析
原文传递
VECTOR ROGUE WAVE PATTERNS OF THE MULTI-COMPONENT NONLINEAR SCHR?DINGER EQUATION AND GENERALIZED MIXED ADLER-MOSER POLYNOMIALS
2
作者 Huian LIN Liming LING 《Acta Mathematica Scientia》 2025年第6期2478-2509,共32页
This paper investigates the asymptotic behavior of high-order vector rogue wave(RW)solutions for any multi-component nonlinear Schr¨odinger equation(denoted as n-NLSE)with multiple internal large parameters.We re... This paper investigates the asymptotic behavior of high-order vector rogue wave(RW)solutions for any multi-component nonlinear Schr¨odinger equation(denoted as n-NLSE)with multiple internal large parameters.We report some novel RW patterns,including nonmultiple root(NMR)-type patterns with distinct shapes such as semicircular sector,acute sector,pseudo-hexagram,and pseudo-rhombus shapes,as well as multiple root(MR)-type patterns characterized by right double-arrow and right arrow shapes.We demonstrate that these RW patterns are intrinsically related to the root structures of a novel class of polynomials,termed generalized mixed Adler-Moser(GMAM)polynomials,which feature multiple arbitrary free parameters.The RW patterns can be interpreted as straightforward expansions and slight shifts of the root structures for the GMAM polynomials to some extent.In the(x,t)-plane,they asymptotically converge to a first-order RW at the location corresponding to each simple root of the polynomials and to a lower-order RW at the location associated with each multiple root.Notably,the position of the lower-order RW within these patterns can be flexibly adjusted to any desired location in the(x,t)-plane by tuning the free parameters of the corresponding GMAM polynomials. 展开更多
关键词 n-NLSE RW pattern ASYMPTOTICS gmam polynomial root structure
在线阅读 下载PDF
Generation of male-sterile soybean lines with the CRISPR/Cas9 system 被引量:6
3
作者 Xiao Chen Suxin Yang +5 位作者 Yaohua Zhang Xiaobin Zhu Xinjing Yang Chunbao Zhang Haiyan Li Xianzhong Feng 《The Crop Journal》 SCIE CSCD 2021年第6期1270-1277,共8页
Soybean [Glycine max(L.) Merr.] provides a rich source of plant protein and oil worldwide. The commercial use of transgenic technology in soybean has become a classical example of the application of biotechnology to c... Soybean [Glycine max(L.) Merr.] provides a rich source of plant protein and oil worldwide. The commercial use of transgenic technology in soybean has become a classical example of the application of biotechnology to crop improvement. Although genetically modified soybeans have achieved commercial success,hybrid soybean breeding is also a potential way to increase soybean yield. Soybean cytoplasmic malesterile(CMS) lines have been used in three-line hybrid breeding systems, but their application to exploiting soybean heterosis has been limited by rare germplasm resource of sterile lines. The generation of various genetic diversity male-sterile soybean lines will help to overcome the shortcoming. In this study,we used targeted editing of AMS homologs in soybean by CRISPR/Cas9 technology for the first time to generate stable male-sterile lines. Targeted editing of GmAMS1 resulted in a male-sterile phenotype,while editing of GmAMS2 failed to produce male-sterile lines. GmAMS1 functions not only in the formation of the pollen wall but also in the controlling the degradation of the soybean tapetum.CRISPR/Cas9 technology could be used to rapidly produce stable male-sterile lines, providing new sterile-line materials for soybean hybrid breeding systems. 展开更多
关键词 CRISPR/Cas9 SOYBEAN TAPETUM gmams Male sterility
在线阅读 下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部