This study aimed to analyze the effect of lipid peroxidation on the allergenicity and functional properties of soybeanβ-conglycinin(7 S)and glycinin(11 S).Oxidation complexes were determined using the lipid peroxidat...This study aimed to analyze the effect of lipid peroxidation on the allergenicity and functional properties of soybeanβ-conglycinin(7 S)and glycinin(11 S).Oxidation complexes were determined using the lipid peroxidation method.Functional properties were analyzed based on emulsifying and foaming properties.The potential allergenicity was evaluated by in vitro and in vivo methods.The results found that oxidation altered structures of the proteins and resulted in the formation of cross-linked protein polymers.The emulsion and foaming properties of the proteins were improved after oxidation.The IgE-binding capacity of 7 S and11 S reduced after oxidation.KU812 cell assays showed that both histamine and IL-4 release decreased after oxidation treatment.A mouse model showed that oxidation reduced the IgE,IgG,and IgG1 levels,as well as reduced histamine and mMCP-1 release in serum,which might suppress the allergic reaction.In conclusion,the lipid peroxidation treatment likely causes changes to the functional properties of soybean,decreasing the potential allergenicity of 7 S and 11 S.展开更多
The secondary structures of soybean glycinin was investigated by Raman spectroscopy and its acidic and basic polypeptides were isolated. The results showed that the secondary structures of glycinin were mainly compose...The secondary structures of soybean glycinin was investigated by Raman spectroscopy and its acidic and basic polypeptides were isolated. The results showed that the secondary structures of glycinin were mainly composed of 21.51% α-helix, 41.62% β-sheet,24.70% β-turn, and 12.18% random coil. For the disulfide bridge(—S—S—), the ratios were 34.8% gauche—gauche—gauche(g—g—g), 32.1% gauche—gauche—trans(g—g—t), and 33.1% trans-gauche-trans(t—g—t). The I_(850)/I_(830) intensity ratio of glycinin Raman tyrosine doublet confirmed that the contents of the N-buried and N-exposed tyrosine residue were 14.1% and 85.9%,respectively. The typical acidic subunit A and basic subunit B were clearly separated by heat denaturation and reduction withβ-mercaptoethanol, and their corresponding molecular masses were 42 and 38 ku, respectively. Raman spectroscopic analysis can be used to determine the secondary structural properties of glycinin. Further studies of the glycinin structures will be helpful for the utilization of soybean protein resources.展开更多
Soy glycinin derived octapeptide(SGP8)is a peptide obtained from degradation of the soy glycinin,whose amino acid sequence is IAVPGEVA.To determine the effect of SGP8 on non-alcoholic fatty liver disease(NAFLD),steato...Soy glycinin derived octapeptide(SGP8)is a peptide obtained from degradation of the soy glycinin,whose amino acid sequence is IAVPGEVA.To determine the effect of SGP8 on non-alcoholic fatty liver disease(NAFLD),steatosis Hep G2 cells were induced by 1 mmol/L free fatty acid(FFA)and C57 BL/6 J mice were fed with methionine-choline defi cient(MCD)diet for 3 weeks to establish NAFLD model.The results of oil red O staining and total cholesterol(TC)/triglyceride(TG)contents showed that SGP8 could signifi cantly reduce the lipid content of steatosis Hep G2 cells.In vivo,SGP8 lowered plasma alanine aminotransferase(ALT)and low density lipoprotein(LDL)content,normalized hepatic superoxide dismutase(SOD)and malondialdehyde(MDA)production,and reduced the severity of liver infl ammation.The results of Western blotting showed that SGP8 increased expression of Sirtuin-1(SIRT1)and phosphorylation level of AMP activated protein kinase(AMPK)in hepatocytes.Through activation of SIRT1/AMPK pathway,SGP8 downregulated the expression of sterol regulatory element binding protein 1 c(SREBP-1 c)and its target genes ACC and FAS expression levels,and increased the phosphorylation level of acetyl Co A carboxylase(ACC).Furthermore,SGP8 also upregulated the expression of transcription factor peroxisome proliferator activated receptorα(PPARα),which was regulated by SIRT1/AMPK pathway,and its target gene CPT1 level.In conclusion,SGP8 might improve NAFLD by activating the SIRT1/AMPK pathway.Our data suggest that SGP8 may act as a novel and potent therapeutic agent against NAFLD.展开更多
[ Objective] This study aimed to characterize the structure of soybean glycinin affected by limited enzymatic hydrolysis. [ Method ] The glycinin was limitedly hydrolyzed by alkaline protease; then the SDS-polyacrylam...[ Objective] This study aimed to characterize the structure of soybean glycinin affected by limited enzymatic hydrolysis. [ Method ] The glycinin was limitedly hydrolyzed by alkaline protease; then the SDS-polyacrylamide gel electrophoresis （SDS-PAGE）, scanning electron microscopy （SEM）, Fourier transform infrared spectroscopy （fTIR） and other means were performed to characterize the glyeinin structure changing during the hydrolysis process. [ Result] SDS-PAGE analysis showed that the subunit contem of glycinin significantly decreased after hydrolysis, and acidic subunits were more susceptible to hydrolysis than alkaline subunits. The scanning electron microscopy revealed that the structure of glycinin powder changed greatly after hydrolysis. The I^TIR results showed that the propor- tions of all conformations of glycinin changed greatly during hydrolysis process. In addition, the protein hydrophobicity and sulfhydryl content were also significantly influenced by hydrolysis. [ Conclusion] The enzymatic hydrolysis greatly changed the conformations of glyeinin, and the change was dependent on the degree of hy- drolysis.展开更多
The utilization efficiency of soy protein is affected by its 2 anti-nutritional substancesdthe antigens bconglycinin and glycinin.This study investigated their effects on the growth performance,intestinal immune defen...The utilization efficiency of soy protein is affected by its 2 anti-nutritional substancesdthe antigens bconglycinin and glycinin.This study investigated their effects on the growth performance,intestinal immune defense,andmicrobiome in juvenile pearl gentian groupers(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂).Three isonitrogenous and isolipidic diets were formulated containing fishmeal supplemented with 70 g/kgβ-conglycinin or 100 g/kg glycinin,or no supplementation(control).Each experimental diet was fed to quadruplicate groups with 30 fish in each tank for 8 weeks.Dietary inclusion of eitherβ-conglycinin or glycinin significantly reduced weight gain and specific growth rates,and cell proliferation of the distal intestine.Histological evaluation of the intestine tract revealed the inflammation signs,characterized by reducing of plica height and width as well as the number of the goblet cells,and widening of the lamina propria.The group fed theβ-conglycinin diet had reduced lysozyme activity,contents of immunoglobulin M and complements 3 and 4.Increased activities of caspase-3 and-9 were observed in the group fed the bconglycinin diet compared to the other 2 groups.In the intestinal microbiota,the relative abundances of the potentially pathogenic genera Photobacterium and Vibrio were significantly higher in the glycinin group than those in others.Therefore,the existence of soybean antigens(β-conglycinin or glycinin)could damage the structural integrity of the intestine,reduce immune defense,reshape the intestinal microbiome and,ultimately,impair growth in fish.展开更多
基金supported in part by the National Natural Science Foundation of China(32172311)Key-Area Research and Development Program of Guangdong Province(2019B020213001)+1 种基金Guangdong Basic and Applied Basic Research Foundation(2021A1515012413)the support from the Instrumental Analysis Center of Shenzhen University(Xili Campus)。
文摘This study aimed to analyze the effect of lipid peroxidation on the allergenicity and functional properties of soybeanβ-conglycinin(7 S)and glycinin(11 S).Oxidation complexes were determined using the lipid peroxidation method.Functional properties were analyzed based on emulsifying and foaming properties.The potential allergenicity was evaluated by in vitro and in vivo methods.The results found that oxidation altered structures of the proteins and resulted in the formation of cross-linked protein polymers.The emulsion and foaming properties of the proteins were improved after oxidation.The IgE-binding capacity of 7 S and11 S reduced after oxidation.KU812 cell assays showed that both histamine and IL-4 release decreased after oxidation treatment.A mouse model showed that oxidation reduced the IgE,IgG,and IgG1 levels,as well as reduced histamine and mMCP-1 release in serum,which might suppress the allergic reaction.In conclusion,the lipid peroxidation treatment likely causes changes to the functional properties of soybean,decreasing the potential allergenicity of 7 S and 11 S.
基金Supported by Grain&Corn Engineering Technology Research Center,State Administration of Grain(GA2017004)Science and Technology Research Project of Henan(172102110205)
文摘The secondary structures of soybean glycinin was investigated by Raman spectroscopy and its acidic and basic polypeptides were isolated. The results showed that the secondary structures of glycinin were mainly composed of 21.51% α-helix, 41.62% β-sheet,24.70% β-turn, and 12.18% random coil. For the disulfide bridge(—S—S—), the ratios were 34.8% gauche—gauche—gauche(g—g—g), 32.1% gauche—gauche—trans(g—g—t), and 33.1% trans-gauche-trans(t—g—t). The I_(850)/I_(830) intensity ratio of glycinin Raman tyrosine doublet confirmed that the contents of the N-buried and N-exposed tyrosine residue were 14.1% and 85.9%,respectively. The typical acidic subunit A and basic subunit B were clearly separated by heat denaturation and reduction withβ-mercaptoethanol, and their corresponding molecular masses were 42 and 38 ku, respectively. Raman spectroscopic analysis can be used to determine the secondary structural properties of glycinin. Further studies of the glycinin structures will be helpful for the utilization of soybean protein resources.
基金funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Soy glycinin derived octapeptide(SGP8)is a peptide obtained from degradation of the soy glycinin,whose amino acid sequence is IAVPGEVA.To determine the effect of SGP8 on non-alcoholic fatty liver disease(NAFLD),steatosis Hep G2 cells were induced by 1 mmol/L free fatty acid(FFA)and C57 BL/6 J mice were fed with methionine-choline defi cient(MCD)diet for 3 weeks to establish NAFLD model.The results of oil red O staining and total cholesterol(TC)/triglyceride(TG)contents showed that SGP8 could signifi cantly reduce the lipid content of steatosis Hep G2 cells.In vivo,SGP8 lowered plasma alanine aminotransferase(ALT)and low density lipoprotein(LDL)content,normalized hepatic superoxide dismutase(SOD)and malondialdehyde(MDA)production,and reduced the severity of liver infl ammation.The results of Western blotting showed that SGP8 increased expression of Sirtuin-1(SIRT1)and phosphorylation level of AMP activated protein kinase(AMPK)in hepatocytes.Through activation of SIRT1/AMPK pathway,SGP8 downregulated the expression of sterol regulatory element binding protein 1 c(SREBP-1 c)and its target genes ACC and FAS expression levels,and increased the phosphorylation level of acetyl Co A carboxylase(ACC).Furthermore,SGP8 also upregulated the expression of transcription factor peroxisome proliferator activated receptorα(PPARα),which was regulated by SIRT1/AMPK pathway,and its target gene CPT1 level.In conclusion,SGP8 might improve NAFLD by activating the SIRT1/AMPK pathway.Our data suggest that SGP8 may act as a novel and potent therapeutic agent against NAFLD.
基金Supported by 863 Program(2006AA10Z330)New Century High-level Talent Project in Hubei Province[(2003)31]
文摘[ Objective] This study aimed to characterize the structure of soybean glycinin affected by limited enzymatic hydrolysis. [ Method ] The glycinin was limitedly hydrolyzed by alkaline protease; then the SDS-polyacrylamide gel electrophoresis （SDS-PAGE）, scanning electron microscopy （SEM）, Fourier transform infrared spectroscopy （fTIR） and other means were performed to characterize the glyeinin structure changing during the hydrolysis process. [ Result] SDS-PAGE analysis showed that the subunit contem of glycinin significantly decreased after hydrolysis, and acidic subunits were more susceptible to hydrolysis than alkaline subunits. The scanning electron microscopy revealed that the structure of glycinin powder changed greatly after hydrolysis. The I^TIR results showed that the propor- tions of all conformations of glycinin changed greatly during hydrolysis process. In addition, the protein hydrophobicity and sulfhydryl content were also significantly influenced by hydrolysis. [ Conclusion] The enzymatic hydrolysis greatly changed the conformations of glyeinin, and the change was dependent on the degree of hy- drolysis.
基金the National Key R&D Program of China(2019YFD0900200)the China Agriculture Research System of MOFand MARA(CARS-47)+1 种基金the National Natural Science Foundation of China(NSFC 31772864)the Fund of Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang)(ZJW-2019-06)。
文摘The utilization efficiency of soy protein is affected by its 2 anti-nutritional substancesdthe antigens bconglycinin and glycinin.This study investigated their effects on the growth performance,intestinal immune defense,andmicrobiome in juvenile pearl gentian groupers(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂).Three isonitrogenous and isolipidic diets were formulated containing fishmeal supplemented with 70 g/kgβ-conglycinin or 100 g/kg glycinin,or no supplementation(control).Each experimental diet was fed to quadruplicate groups with 30 fish in each tank for 8 weeks.Dietary inclusion of eitherβ-conglycinin or glycinin significantly reduced weight gain and specific growth rates,and cell proliferation of the distal intestine.Histological evaluation of the intestine tract revealed the inflammation signs,characterized by reducing of plica height and width as well as the number of the goblet cells,and widening of the lamina propria.The group fed theβ-conglycinin diet had reduced lysozyme activity,contents of immunoglobulin M and complements 3 and 4.Increased activities of caspase-3 and-9 were observed in the group fed the bconglycinin diet compared to the other 2 groups.In the intestinal microbiota,the relative abundances of the potentially pathogenic genera Photobacterium and Vibrio were significantly higher in the glycinin group than those in others.Therefore,the existence of soybean antigens(β-conglycinin or glycinin)could damage the structural integrity of the intestine,reduce immune defense,reshape the intestinal microbiome and,ultimately,impair growth in fish.
