Glycidol is a common lipid-derived foodborne toxicant mainly presents in refined oils and related foodstuffs.Vascular endothelial cells may be potential targets of the deleterious effects associated with glycidol expo...Glycidol is a common lipid-derived foodborne toxicant mainly presents in refined oils and related foodstuffs.Vascular endothelial cells may be potential targets of the deleterious effects associated with glycidol exposure.In human umbilical vein endothelial cells(HUVECs),we found that glycidol treatment promoted endothelialto-mesenchymal transition(EndMT)at a lower concentration(0.5 mmol/L),while induced apoptosis and inflammation at a higher concentration(1 mmol/L).These harmful effects were achieved by the activation of NF-κB/MAPK signaling pathway and were mediated by reactive oxygen species(ROS).In addition,the protective potential of 6-C-(E-2-fluorostyryl)naringenin(6-CEFN)against glycidol was evaluated and compared with naringenin.HUVECs pre-treated with 6-CEFN,but not naringenin,displayed resistance to endothelial dysfunction caused by glycidol.展开更多
Enantioselective transesterification of glycidol with vinyl butyrate as an acyl donor was investigated in the presence of Bacillus subtilis lipase（BSL2） as catalyst. Comparison studies demonstrate the advantage of u...Enantioselective transesterification of glycidol with vinyl butyrate as an acyl donor was investigated in the presence of Bacillus subtilis lipase（BSL2） as catalyst. Comparison studies demonstrate the advantage of ultrasound over the conventional shaking for the enzymatic reaction in non-aqueous media. The effects of reaction conditions（ultrasound power, temperature, water activity and pH） on the activity and enantioselectivity were also investigated. Under the optimum conditions, the synthetic activity of BSL2 was 2.95 μmol？min？1？mg？1 and the enantioselectivity（E value） was 52.2. Compared with conventional shaking, ultrasound made the synthetic activity and the enantioselectivity increase 9.5-fold and 1.4-fold, respectively. Furthermore, the repeated use of BSL2 for five cycles resulted in no obvious loss of enzyme activity, suggesting that the enzyme is stable under low power ultrasound conditions.展开更多
A series of linear poly glycidol copolymers, tethering with both alkene and hydroxyl groups, were prepared by a combination of anionic ring-opening polymerization （ROP） using specific reactions of ethoxy ethyl glyci...A series of linear poly glycidol copolymers, tethering with both alkene and hydroxyl groups, were prepared by a combination of anionic ring-opening polymerization （ROP） using specific reactions of ethoxy ethyl glycidyl ether （EEGE） and allyl glycidyl ether （AGE） firstly, and subsequently removal of the protection group of glycidol in EEGE to achieve the linear copolymer pendant with both hydroxyl groups and double bonds. The EEGE/AGE monomer reactivity ratio is measured to be 3.30/1.13. The chemical compositions of the as-synthesized polymers were characterized by tH NMR and GPC, and the glass transition temperatures （Tg） of as-synthesized polymers were determined by DSC. The final copolymers have abundant double bonds and hydroxyl as side groups. Furthermore, the ratio of the double bonds to hydroxyl groups can be controlled by the ratio of the starting materials in a wide range.展开更多
甘油是生物柴油生产中的一种重要副产品,将之转化为具有更高附加值的缩水甘油是一种重要的甘油消化途径。缩水甘油分子中含有环氧和羟基双官能团,可用于环氧树酯、聚氨酯以及各种新型应激响应性高分子材料的合成工作,是一种重要的化工...甘油是生物柴油生产中的一种重要副产品,将之转化为具有更高附加值的缩水甘油是一种重要的甘油消化途径。缩水甘油分子中含有环氧和羟基双官能团,可用于环氧树酯、聚氨酯以及各种新型应激响应性高分子材料的合成工作,是一种重要的化工原料。论文从碳酸甘油酯出发,在减压条件下使用无水硫酸钠为主体的复合催化剂,经高温脱二氧化碳制备缩水甘油。实验研究了反应温度,反应物配比,催化剂种类及用量对缩水甘油收率的影响,使用红外和核磁碳谱对产品进行了表征。实验结果表明,在添加了酸性助催化剂磷酸二氢钾和磷酸时,缩水甘油的产率比单纯使用无水硫酸钠有较大增长,在使用15.5 g无水硫酸钠和0.5 g 85%磷酸组成的复合催化剂情况下,缩水甘油产率可达89%。展开更多
基金supported by the National Key R&D Program of China(2021YFD2100103)the National Natural Science Foundation of China(32101935).
文摘Glycidol is a common lipid-derived foodborne toxicant mainly presents in refined oils and related foodstuffs.Vascular endothelial cells may be potential targets of the deleterious effects associated with glycidol exposure.In human umbilical vein endothelial cells(HUVECs),we found that glycidol treatment promoted endothelialto-mesenchymal transition(EndMT)at a lower concentration(0.5 mmol/L),while induced apoptosis and inflammation at a higher concentration(1 mmol/L).These harmful effects were achieved by the activation of NF-κB/MAPK signaling pathway and were mediated by reactive oxygen species(ROS).In addition,the protective potential of 6-C-(E-2-fluorostyryl)naringenin(6-CEFN)against glycidol was evaluated and compared with naringenin.HUVECs pre-treated with 6-CEFN,but not naringenin,displayed resistance to endothelial dysfunction caused by glycidol.
基金Supported by the National Natural Science Foundation of China(Nos.20803028, 31070708, 30870539)the Scientific Research Fund of Jilin University, China(No.200903099)the Graduate Innovation Fund of Jilin University, China (No.20091013)
文摘Enantioselective transesterification of glycidol with vinyl butyrate as an acyl donor was investigated in the presence of Bacillus subtilis lipase（BSL2） as catalyst. Comparison studies demonstrate the advantage of ultrasound over the conventional shaking for the enzymatic reaction in non-aqueous media. The effects of reaction conditions（ultrasound power, temperature, water activity and pH） on the activity and enantioselectivity were also investigated. Under the optimum conditions, the synthetic activity of BSL2 was 2.95 μmol？min？1？mg？1 and the enantioselectivity（E value） was 52.2. Compared with conventional shaking, ultrasound made the synthetic activity and the enantioselectivity increase 9.5-fold and 1.4-fold, respectively. Furthermore, the repeated use of BSL2 for five cycles resulted in no obvious loss of enzyme activity, suggesting that the enzyme is stable under low power ultrasound conditions.
基金Acknowledgement This study was supported by the National Natural Science Foundation of China （No. 50903096）, the Department of Science Technology of Guangdong Province （No. 2008B090500196）, and the Fundamental Research Funds for the Central Universities.
文摘A series of linear poly glycidol copolymers, tethering with both alkene and hydroxyl groups, were prepared by a combination of anionic ring-opening polymerization （ROP） using specific reactions of ethoxy ethyl glycidyl ether （EEGE） and allyl glycidyl ether （AGE） firstly, and subsequently removal of the protection group of glycidol in EEGE to achieve the linear copolymer pendant with both hydroxyl groups and double bonds. The EEGE/AGE monomer reactivity ratio is measured to be 3.30/1.13. The chemical compositions of the as-synthesized polymers were characterized by tH NMR and GPC, and the glass transition temperatures （Tg） of as-synthesized polymers were determined by DSC. The final copolymers have abundant double bonds and hydroxyl as side groups. Furthermore, the ratio of the double bonds to hydroxyl groups can be controlled by the ratio of the starting materials in a wide range.
文摘甘油是生物柴油生产中的一种重要副产品,将之转化为具有更高附加值的缩水甘油是一种重要的甘油消化途径。缩水甘油分子中含有环氧和羟基双官能团,可用于环氧树酯、聚氨酯以及各种新型应激响应性高分子材料的合成工作,是一种重要的化工原料。论文从碳酸甘油酯出发,在减压条件下使用无水硫酸钠为主体的复合催化剂,经高温脱二氧化碳制备缩水甘油。实验研究了反应温度,反应物配比,催化剂种类及用量对缩水甘油收率的影响,使用红外和核磁碳谱对产品进行了表征。实验结果表明,在添加了酸性助催化剂磷酸二氢钾和磷酸时,缩水甘油的产率比单纯使用无水硫酸钠有较大增长,在使用15.5 g无水硫酸钠和0.5 g 85%磷酸组成的复合催化剂情况下,缩水甘油产率可达89%。
