In order to adapt to the low temperature environment,organisms transmit excitement to the central system through the thermal sensing system,which is a classic reflex reaction.The cold receptor GLR-3 perceives cold and...In order to adapt to the low temperature environment,organisms transmit excitement to the central system through the thermal sensing system,which is a classic reflex reaction.The cold receptor GLR-3 perceives cold and produces cold avoidance behavior through peripheral sensory neurons ASER.In order to further understand the gene encoding of the cold sensing glr-3 gene and the evolution of its homologous gene group function and protein function,the nucleotide sequence and amino acid sequence of the glr-3 gene and its homologous gene in 24 species were obtained and compared.By clustering with the GRIK2 gene sequence of Rana chensinensis,the bioinformatics method was used to predict and sequence analyze the change of gene,evolution rate,physical and chemical properties of protein,glycosylation sites,phosphorylation sites,secondary structure and tertiary structure of protein.The analysis results show that the glr-3 gene and its homologous gene have obvious positive selection effect.The protein prediction analysis showed that the glr-3 gene and its homologous genes encoded proteins in these 25 species were hydrophilic proteins,and the proportion of side chains of aliphatic amino acids was high.The transmembrane helix was widespread and there were more N-glycosylation sites and O-glycosylation sites.The protein phosphorylation sites encoded were serine,threonine and tyrosine phosphorylation sites.Secondary structure prediction showed that the secondary structure units of the encoded protein wereα-helix,β-turn,random coil and extended chain,and the proportion ofα-helix was the largest.This study provides useful information on the evolution and function of the cold sensing gene glr-3 and its homologous genes.展开更多
基金This study was funded by Heilongjiang Province College Students'Innovation Training Program of Northeast Forestry University(S202010225095).
文摘In order to adapt to the low temperature environment,organisms transmit excitement to the central system through the thermal sensing system,which is a classic reflex reaction.The cold receptor GLR-3 perceives cold and produces cold avoidance behavior through peripheral sensory neurons ASER.In order to further understand the gene encoding of the cold sensing glr-3 gene and the evolution of its homologous gene group function and protein function,the nucleotide sequence and amino acid sequence of the glr-3 gene and its homologous gene in 24 species were obtained and compared.By clustering with the GRIK2 gene sequence of Rana chensinensis,the bioinformatics method was used to predict and sequence analyze the change of gene,evolution rate,physical and chemical properties of protein,glycosylation sites,phosphorylation sites,secondary structure and tertiary structure of protein.The analysis results show that the glr-3 gene and its homologous gene have obvious positive selection effect.The protein prediction analysis showed that the glr-3 gene and its homologous genes encoded proteins in these 25 species were hydrophilic proteins,and the proportion of side chains of aliphatic amino acids was high.The transmembrane helix was widespread and there were more N-glycosylation sites and O-glycosylation sites.The protein phosphorylation sites encoded were serine,threonine and tyrosine phosphorylation sites.Secondary structure prediction showed that the secondary structure units of the encoded protein wereα-helix,β-turn,random coil and extended chain,and the proportion ofα-helix was the largest.This study provides useful information on the evolution and function of the cold sensing gene glr-3 and its homologous genes.
