This study investigates the nitrogen (N), phosphorous (P), and potassium (K) contents in raw biomasses of Camellia sinensis, Gliricidia sepium, and Musa acuminata. Therein, the highest N and P content was seen in Came...This study investigates the nitrogen (N), phosphorous (P), and potassium (K) contents in raw biomasses of Camellia sinensis, Gliricidia sepium, and Musa acuminata. Therein, the highest N and P content was seen in Camellia sinensis 116.80 ± 0.08 mg and 66.00 ± 0.14 mg respectively. The highest K content (106.80 ± 0.04 mg) was observed in Musa acuminata. Next, all three types of plant materials were allowed to decompose in water for 3 weeks, and a sample from each was analyzed for NPK after the 1st, 2nd, and 3rd week during decomposition. A significant increase in the release of N, P, and K by the Camellia sinensis to water (P Musa acuminate were not significantly changed (P > 0.05) over time. The ratio for N:P:K was calculated for raw biomass samples and decomposed samples to find the best fitting N:P:K ratio to apply to young tea plants as organic fertilizers. In addition to that, the microbial insight of these organic compounds was analyzed by observing how microbial population increased with decomposition by the enumeration of the total microbial count. A considerable increment in total microbial count was observed up to 3.28 × 10<sup>6</sup>, 1.21 × 10<sup>10</sup>, 2.18 × 10<sup>8</sup>, and 6.49 × 10<sup>7</sup> CFU/ml for Camellia sinensis, Gliricidia sepium, Musa accuminata (leaves), and Musa accuminata (trunk) respectively. The presence of phosphate solubilizing bacteria (PSB) and nitrogen solubilizing bacteria (NSB) throughout the decomposition period was confirmed by their growth on NBRIP and a modified nutrient medium that was specifically designed for the identification of ammonifiers respectively. Prepared fertilizer samples were applied to young tea plants that were grown in the Mawanella area in Sri Lanka (7°15'12.42"N 80°26'47.62"E) and according to the results, it is clear that fertilizer mixture 1 (N:P:K, 10:5:10, tea dust + Gliricidia + banana trunk) and fertilizer mixture 2 (N:P:K, 10:5:10, tea dust + Gliricidia + banana leaves) has the potential to increase the growth of young tea plants.展开更多
Objective:To assess ovicidal activity of three acetonic extracts from the leaves of three forage legume,Calliandra calotyrsus(C.calotyrsus),Gliricidia sepium(G.sepium) and Leucaena diversifolia(L.diversifolia) in vitr...Objective:To assess ovicidal activity of three acetonic extracts from the leaves of three forage legume,Calliandra calotyrsus(C.calotyrsus),Gliricidia sepium(G.sepium) and Leucaena diversifolia(L.diversifolia) in vitro on Haemonchus contortus(H.contortus).Methods:Eggs were exposed for 24 hours to five different concentrations(0.075,0.15,0.3,0.6 and 1.25 mg/mL) of acetonic extracts at room temperature(24℃).Distilled water and 0.4%Tween were used in the bioassay as negative controls.Results:The later did not affect embryonation and egg hatching of H.contortus.Conversively,significant effects were obtained with the acetonic extracts of leaves of all three plants and the maximum activity was observed with the highest concentration (1.25 mg/mL).The acetonic extract of G.sepium was found to be more active(2.9%and 0.0% for embryonation and egg hatching,respectively) than the other substances 16.5%and 33.5%, respectively for C.calothyrsus,33.7%and 33.3%,respectively for L diversifolia.Conclusions: These results suggest that the three forage legumes do possess ovicidal properties and further studies on larvae should be carried out.展开更多
Objective:To investigate the potentiality of mosquitocidal activity of Gliricidia sepium（C.sepium） （Jacq.）（Leguminosae）.Methods:Twenty five early third instar larvae of Anopheles stephensi （An.stephensi） were ...Objective:To investigate the potentiality of mosquitocidal activity of Gliricidia sepium（C.sepium） （Jacq.）（Leguminosae）.Methods:Twenty five early third instar larvae of Anopheles stephensi （An.stephensi） were exposed to various concentrations（50-250 ppm） and the 24 h LC<sub>50</sub> values of the G.sepium extract was determined by probit analysis.The ovicidal activity was determined against An.stephensi to various concentrations ranging from 25-100 ppm under laboratory conditions.The eggs hatchabilily was assessed 48 h post treatment.The pupicidal activity was determined against An.stephensi to various concentrations ranging from 25-100 ppm.Mortality of each pupa was recorded after 24 h of exposure to the extract.Results:Results pertaining to the experiment clearly revealed that ethanol extract showed significant larvicidal,ovicidal and pupicidal activity against the An.stephensi.Larvicidal activity of ethanol extracts of G.sepium showed maximum mortality in 250 ppm concentration（96.0±2.4）%.Furthermore,the LC<sub>50</sub> was found to be 121.79 and the LC<sub>50</sub> value was recorded to be 231.98 ppm.Ovicidal activity of ethanol extract was assessed by assessing the egg hatchabilily.Highest concentration of both solvent extracts exhibited 100%ovicidal activity.Similarly,pupae exposed to different concentratioiLs of ethanol extract were found dead with 58.10%adult emergence when it was treated with 25 ppm concentration.Similarly,18.36（n=30;61.20%）;21.28（70.93） and 27.33（91.10） pupal mortality was recorded from the experimental pupae treated with 50,75 and 100 ppm concentration of extracts. Three fractions have been tested for their larvicidal activity of which the Fraction 3 showed the LC<sub>50</sub> and LC<sub>90</sub> values of 23.23 and 40.39 ppm.With regard to the ovicidal effect fraction 3 showed highest ovicidal activities than the other two fractions.Furthermore,there were no hatchabilily was recorded above 50 ppm（100%egg mortality） in the experimental group.Statistically significant pupicidal activity was recorded from 75 ppm concentration.Conclusions:From the results it can be concluded the crude extract of G.sepium is an excellent potential for controlling An.stephensi mosquito.It is apparent that,iraction 3 possess a novel and active principle which could be responsible for those biological activities.展开更多
Fermented Callliandra, Gliricidia and Leucaena browses and maize material （milk stage） were applied to the soil to determine their effect on soil productivity. Hopi Red Dye Amaranthus （Amaranthus cruentus） was use...Fermented Callliandra, Gliricidia and Leucaena browses and maize material （milk stage） were applied to the soil to determine their effect on soil productivity. Hopi Red Dye Amaranthus （Amaranthus cruentus） was used as the test crop. Its DM yield was determined. The browse materials had higher total N and narrower C：N ratio than the maize material. Calliandra material had higher levels of insoluble fiber （ADF）, fiber bound N （ADFN） and lignin. Application of the browse and maize materials raised C, N, and C：N ratio of the soil in which they were applied compared to the control soil. The treated soils maintained higher levels of C and N and a narrower C：N ratio up to the third crop. Amaranthus DM yield was highest （P 〈 0.05） with browse treatments. Treatment with maize material did not have DM yield advantage over the control soil. Treatments with browse materials could have given higher yields because the materials had more N and fermentation could not only have increased the proportion of soluble N, but also degradability of the materials, thus making the N and other nutrients in the fermented materials available for plant growth. DM yield was highest （P 〈_ 0.05） with the first and third crops but lowest （P 〈 0.05） with the second crop. Plant growth in the second crop could have coincided with high demand for the N by the soil micro-organisms decomposing the added materials. During the third crop, decomposition of the readily degradable components of the added materials could have been complete and the N became available for plant growth. DM yield dropped （P 〈 0.05） with the fourth crop as the available N could have been depleted from the soil for plant growth.展开更多
A low liveweight gain of of Kacang goats when given grass as single feed indicated that additional feed is required to increase their gowth performance. This study was done to examine the effect of addition of Glirici...A low liveweight gain of of Kacang goats when given grass as single feed indicated that additional feed is required to increase their gowth performance. This study was done to examine the effect of addition of Gliricidia （Gliricidia sepium） leaf or rice bran on feed intake, feed digestibility and liveweight gain of Kacang goat given Mulato （Brachiaria mulato） grass. Twenty four male Kacang goat, approximately 13.4±0.6 kg in initial weight and 10 months of age, were housed in individual metabolic pen and allocated to one of three dietary treatments. The experimental design was a completely randomized block design, with three treatments and eight replicates （goats）. The treatments were Mulato grass ad libitum （M）, Mulato ＋ Gliricidia （1% weight/d on dry matter basis） （MG）, and Mulato ＋ rice bran （1% weight/d on dry matter basis） （MR）. The experiment was run for eight weeks period （two weeks for adaptation and and six weeks for measurement period, respectively）. Parameters measured were liveweight gain （LWG）, dry matter intake （DMI） and dry matter digestibility （DMD）. The result showed that dietary treatment significantly （P 〈 0.05） affected the LWG, DMI and DMD of Kacang goat. Goat given Mulato grass had the lowest LWG, DMI and DMD with value 58 g/d, 2.77% weight/d and 57.61%, respectively. Goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran had a significant higher LWG （73 g/d and 76 g/d, respectively）, DMI （3.25% weight/d and 3.65% weight/d, respectively） and DMD （62.28% and 63.19%, respectively）. However, no significant difference （P 〉 0.05） between goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran. In conclusion, this study demonstrated that protein or energy contents of the diet influenced growth, feed intake and digestibility of Kacang goat.展开更多
Soil acidification and phosphorus deficiency are a major constraint to crop production in tropical soils. Use of conventional liming materials is associated with some limitations viz： inability to solely improve nitr...Soil acidification and phosphorus deficiency are a major constraint to crop production in tropical soils. Use of conventional liming materials is associated with some limitations viz： inability to solely improve nitrogen and available phosphorus in soils, loss of soil organic carbon and soil aggregate stability. Liming and fertilizer potentials of leaves from three plant materials （Tithonia diversifolia （TL）, Imperata cylindrica （SG） and Gliricidia sepium （GL）） widely growing in Ogbomoso, southwest Nigeria, were tested under incubation condition. Each of the plant material was applied at.the rate of 10 t·hm-2 with and without 50% concentration of NPK 15 ： 15 ： 15-urea mix in 500 g acidic soiL Sole lime applied at 1 t·hm-2, sole NPK 15 ： 15 ： 15 applied at 60 kg·hm2 mixed with urea at 60 kg N· hm2 and an unamended soil were compared in completely randomized design in three replicates. The treated soils were incubated for 12 weeks. Thereafter, maize seeds were raised in each pot for a period of 3 weeks. Data coUected were subjected to analysis of variance. Regression analysis was used to predict contributions of increased soil pH in plant material treated soils to exchangeable A1, H, dry root weight of maize and available phosphorus. Results indicated that sole plant materials were the order SG〉TL〉GL significantly （P〈0.05） reduced exchangeable acidity compared to unamended and sole NPK. Sole NPK had the highest exchangeable acidity （4.7 cmol·kg-1） compared to unamended soil （3.3 cmol·kg-1） and sole lime （2.7 cmol·kg-1）. Application of sole Tithonia diversifolia increased available phosphorus by 214% and 97% compared to unamended and sole NPK treated soils respectively. Sole plant materials increased maize root weight by 33% compared to sole NPK. Increasing soil pH at harvesting in plant material treated soils significantly reduced exchangeable H and A1. Soil pH was responsible for up to 33% and 53% reductions in exchangeable Al and H, respectively. This culminated into up to 22% increases in dry root weight of maize seedling. Present results showed ability of the plant materials tested to ameliorate soil acidity and improved soil available phosphorus. The plant materials should be explored for using as green manure and composting feedstock. It will go a long way to reduce high dosage use of conventional liming and fertilizer materials on acidic nutrient degraded soils.展开更多
The research aimed to determine the effect of feed supplements of cassava （Manihot esculenta Crantz） leaves, gliricidia （Gliricidia sepeum） leaves and katuk （Sauropus androgynus （L.） Merr.） leaves on the hemat...The research aimed to determine the effect of feed supplements of cassava （Manihot esculenta Crantz） leaves, gliricidia （Gliricidia sepeum） leaves and katuk （Sauropus androgynus （L.） Merr.） leaves on the hematology of lactating buffalo. Four lactating buffaloes in Kanagarian Pamatang Panjang, Sijunjung district, West Sumatera, Indonesia were studied. The experiment used a Latin square design with four treatments and four replications/block. The treatments were control （without supplementary foliage, A）, with cassava leaves （B）, with gliricidia leaves （C） and with katuk leaves （D） each at 5 kg/haead/day. The hematology variables measured were erythrocytes and leucocytes counts, as well as levels of hemoglobin and hematocrit. The results showed that supplementary foliage of 5 kg/head/day of cassava, gliricidia or katuk as leaves significantly increased the erythrocytes count and hemoglobin level, but did not affect the number of leucocytes in lactating buffalo.展开更多
文摘This study investigates the nitrogen (N), phosphorous (P), and potassium (K) contents in raw biomasses of Camellia sinensis, Gliricidia sepium, and Musa acuminata. Therein, the highest N and P content was seen in Camellia sinensis 116.80 ± 0.08 mg and 66.00 ± 0.14 mg respectively. The highest K content (106.80 ± 0.04 mg) was observed in Musa acuminata. Next, all three types of plant materials were allowed to decompose in water for 3 weeks, and a sample from each was analyzed for NPK after the 1st, 2nd, and 3rd week during decomposition. A significant increase in the release of N, P, and K by the Camellia sinensis to water (P Musa acuminate were not significantly changed (P > 0.05) over time. The ratio for N:P:K was calculated for raw biomass samples and decomposed samples to find the best fitting N:P:K ratio to apply to young tea plants as organic fertilizers. In addition to that, the microbial insight of these organic compounds was analyzed by observing how microbial population increased with decomposition by the enumeration of the total microbial count. A considerable increment in total microbial count was observed up to 3.28 × 10<sup>6</sup>, 1.21 × 10<sup>10</sup>, 2.18 × 10<sup>8</sup>, and 6.49 × 10<sup>7</sup> CFU/ml for Camellia sinensis, Gliricidia sepium, Musa accuminata (leaves), and Musa accuminata (trunk) respectively. The presence of phosphate solubilizing bacteria (PSB) and nitrogen solubilizing bacteria (NSB) throughout the decomposition period was confirmed by their growth on NBRIP and a modified nutrient medium that was specifically designed for the identification of ammonifiers respectively. Prepared fertilizer samples were applied to young tea plants that were grown in the Mawanella area in Sri Lanka (7°15'12.42"N 80°26'47.62"E) and according to the results, it is clear that fertilizer mixture 1 (N:P:K, 10:5:10, tea dust + Gliricidia + banana trunk) and fertilizer mixture 2 (N:P:K, 10:5:10, tea dust + Gliricidia + banana leaves) has the potential to increase the growth of young tea plants.
文摘Objective:To assess ovicidal activity of three acetonic extracts from the leaves of three forage legume,Calliandra calotyrsus(C.calotyrsus),Gliricidia sepium(G.sepium) and Leucaena diversifolia(L.diversifolia) in vitro on Haemonchus contortus(H.contortus).Methods:Eggs were exposed for 24 hours to five different concentrations(0.075,0.15,0.3,0.6 and 1.25 mg/mL) of acetonic extracts at room temperature(24℃).Distilled water and 0.4%Tween were used in the bioassay as negative controls.Results:The later did not affect embryonation and egg hatching of H.contortus.Conversively,significant effects were obtained with the acetonic extracts of leaves of all three plants and the maximum activity was observed with the highest concentration (1.25 mg/mL).The acetonic extract of G.sepium was found to be more active(2.9%and 0.0% for embryonation and egg hatching,respectively) than the other substances 16.5%and 33.5%, respectively for C.calothyrsus,33.7%and 33.3%,respectively for L diversifolia.Conclusions: These results suggest that the three forage legumes do possess ovicidal properties and further studies on larvae should be carried out.
基金Higher Authorities for the grant of financial assistance with the Award of University Research Fellowship(Ref.No.No.H5/2009-2010/URF Order of the Vice chancellore,23011/2009)Professor and Head,Department of Zoology,Annamalai University for the laboratory facilities provided.
文摘Objective:To investigate the potentiality of mosquitocidal activity of Gliricidia sepium（C.sepium） （Jacq.）（Leguminosae）.Methods:Twenty five early third instar larvae of Anopheles stephensi （An.stephensi） were exposed to various concentrations（50-250 ppm） and the 24 h LC<sub>50</sub> values of the G.sepium extract was determined by probit analysis.The ovicidal activity was determined against An.stephensi to various concentrations ranging from 25-100 ppm under laboratory conditions.The eggs hatchabilily was assessed 48 h post treatment.The pupicidal activity was determined against An.stephensi to various concentrations ranging from 25-100 ppm.Mortality of each pupa was recorded after 24 h of exposure to the extract.Results:Results pertaining to the experiment clearly revealed that ethanol extract showed significant larvicidal,ovicidal and pupicidal activity against the An.stephensi.Larvicidal activity of ethanol extracts of G.sepium showed maximum mortality in 250 ppm concentration（96.0±2.4）%.Furthermore,the LC<sub>50</sub> was found to be 121.79 and the LC<sub>50</sub> value was recorded to be 231.98 ppm.Ovicidal activity of ethanol extract was assessed by assessing the egg hatchabilily.Highest concentration of both solvent extracts exhibited 100%ovicidal activity.Similarly,pupae exposed to different concentratioiLs of ethanol extract were found dead with 58.10%adult emergence when it was treated with 25 ppm concentration.Similarly,18.36（n=30;61.20%）;21.28（70.93） and 27.33（91.10） pupal mortality was recorded from the experimental pupae treated with 50,75 and 100 ppm concentration of extracts. Three fractions have been tested for their larvicidal activity of which the Fraction 3 showed the LC<sub>50</sub> and LC<sub>90</sub> values of 23.23 and 40.39 ppm.With regard to the ovicidal effect fraction 3 showed highest ovicidal activities than the other two fractions.Furthermore,there were no hatchabilily was recorded above 50 ppm（100%egg mortality） in the experimental group.Statistically significant pupicidal activity was recorded from 75 ppm concentration.Conclusions:From the results it can be concluded the crude extract of G.sepium is an excellent potential for controlling An.stephensi mosquito.It is apparent that,iraction 3 possess a novel and active principle which could be responsible for those biological activities.
文摘Fermented Callliandra, Gliricidia and Leucaena browses and maize material （milk stage） were applied to the soil to determine their effect on soil productivity. Hopi Red Dye Amaranthus （Amaranthus cruentus） was used as the test crop. Its DM yield was determined. The browse materials had higher total N and narrower C：N ratio than the maize material. Calliandra material had higher levels of insoluble fiber （ADF）, fiber bound N （ADFN） and lignin. Application of the browse and maize materials raised C, N, and C：N ratio of the soil in which they were applied compared to the control soil. The treated soils maintained higher levels of C and N and a narrower C：N ratio up to the third crop. Amaranthus DM yield was highest （P 〈 0.05） with browse treatments. Treatment with maize material did not have DM yield advantage over the control soil. Treatments with browse materials could have given higher yields because the materials had more N and fermentation could not only have increased the proportion of soluble N, but also degradability of the materials, thus making the N and other nutrients in the fermented materials available for plant growth. DM yield was highest （P 〈_ 0.05） with the first and third crops but lowest （P 〈 0.05） with the second crop. Plant growth in the second crop could have coincided with high demand for the N by the soil micro-organisms decomposing the added materials. During the third crop, decomposition of the readily degradable components of the added materials could have been complete and the N became available for plant growth. DM yield dropped （P 〈 0.05） with the fourth crop as the available N could have been depleted from the soil for plant growth.
文摘A low liveweight gain of of Kacang goats when given grass as single feed indicated that additional feed is required to increase their gowth performance. This study was done to examine the effect of addition of Gliricidia （Gliricidia sepium） leaf or rice bran on feed intake, feed digestibility and liveweight gain of Kacang goat given Mulato （Brachiaria mulato） grass. Twenty four male Kacang goat, approximately 13.4±0.6 kg in initial weight and 10 months of age, were housed in individual metabolic pen and allocated to one of three dietary treatments. The experimental design was a completely randomized block design, with three treatments and eight replicates （goats）. The treatments were Mulato grass ad libitum （M）, Mulato ＋ Gliricidia （1% weight/d on dry matter basis） （MG）, and Mulato ＋ rice bran （1% weight/d on dry matter basis） （MR）. The experiment was run for eight weeks period （two weeks for adaptation and and six weeks for measurement period, respectively）. Parameters measured were liveweight gain （LWG）, dry matter intake （DMI） and dry matter digestibility （DMD）. The result showed that dietary treatment significantly （P 〈 0.05） affected the LWG, DMI and DMD of Kacang goat. Goat given Mulato grass had the lowest LWG, DMI and DMD with value 58 g/d, 2.77% weight/d and 57.61%, respectively. Goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran had a significant higher LWG （73 g/d and 76 g/d, respectively）, DMI （3.25% weight/d and 3.65% weight/d, respectively） and DMD （62.28% and 63.19%, respectively）. However, no significant difference （P 〉 0.05） between goat received Mulato ＋ Gliricidia and Mulato ＋ rice bran. In conclusion, this study demonstrated that protein or energy contents of the diet influenced growth, feed intake and digestibility of Kacang goat.
文摘Soil acidification and phosphorus deficiency are a major constraint to crop production in tropical soils. Use of conventional liming materials is associated with some limitations viz： inability to solely improve nitrogen and available phosphorus in soils, loss of soil organic carbon and soil aggregate stability. Liming and fertilizer potentials of leaves from three plant materials （Tithonia diversifolia （TL）, Imperata cylindrica （SG） and Gliricidia sepium （GL）） widely growing in Ogbomoso, southwest Nigeria, were tested under incubation condition. Each of the plant material was applied at.the rate of 10 t·hm-2 with and without 50% concentration of NPK 15 ： 15 ： 15-urea mix in 500 g acidic soiL Sole lime applied at 1 t·hm-2, sole NPK 15 ： 15 ： 15 applied at 60 kg·hm2 mixed with urea at 60 kg N· hm2 and an unamended soil were compared in completely randomized design in three replicates. The treated soils were incubated for 12 weeks. Thereafter, maize seeds were raised in each pot for a period of 3 weeks. Data coUected were subjected to analysis of variance. Regression analysis was used to predict contributions of increased soil pH in plant material treated soils to exchangeable A1, H, dry root weight of maize and available phosphorus. Results indicated that sole plant materials were the order SG〉TL〉GL significantly （P〈0.05） reduced exchangeable acidity compared to unamended and sole NPK. Sole NPK had the highest exchangeable acidity （4.7 cmol·kg-1） compared to unamended soil （3.3 cmol·kg-1） and sole lime （2.7 cmol·kg-1）. Application of sole Tithonia diversifolia increased available phosphorus by 214% and 97% compared to unamended and sole NPK treated soils respectively. Sole plant materials increased maize root weight by 33% compared to sole NPK. Increasing soil pH at harvesting in plant material treated soils significantly reduced exchangeable H and A1. Soil pH was responsible for up to 33% and 53% reductions in exchangeable Al and H, respectively. This culminated into up to 22% increases in dry root weight of maize seedling. Present results showed ability of the plant materials tested to ameliorate soil acidity and improved soil available phosphorus. The plant materials should be explored for using as green manure and composting feedstock. It will go a long way to reduce high dosage use of conventional liming and fertilizer materials on acidic nutrient degraded soils.
文摘The research aimed to determine the effect of feed supplements of cassava （Manihot esculenta Crantz） leaves, gliricidia （Gliricidia sepeum） leaves and katuk （Sauropus androgynus （L.） Merr.） leaves on the hematology of lactating buffalo. Four lactating buffaloes in Kanagarian Pamatang Panjang, Sijunjung district, West Sumatera, Indonesia were studied. The experiment used a Latin square design with four treatments and four replications/block. The treatments were control （without supplementary foliage, A）, with cassava leaves （B）, with gliricidia leaves （C） and with katuk leaves （D） each at 5 kg/haead/day. The hematology variables measured were erythrocytes and leucocytes counts, as well as levels of hemoglobin and hematocrit. The results showed that supplementary foliage of 5 kg/head/day of cassava, gliricidia or katuk as leaves significantly increased the erythrocytes count and hemoglobin level, but did not affect the number of leucocytes in lactating buffalo.
