Inheritance analysis and gene mapping of the glabrous trait were conducted using the crosses between a pubescent rice material Chuanxiang 29B （an aromatic elite maintainer line of hybrid rice）, and two glabrous rice...Inheritance analysis and gene mapping of the glabrous trait were conducted using the crosses between a pubescent rice material Chuanxiang 29B （an aromatic elite maintainer line of hybrid rice）, and two glabrous rice materials, Lemont and R2 （R2 is a new restorer line of hybrid rice）. All F1 plants from the two crosses had pubescent leaves, confirming that the pubescence trait in Chuanxiang 29B is dominant over the glabrous trait. In F2 individuals, the segregation ratio of three pubescent to one glabrous plant suggests that a single glabrous gene was present in Lemont and R2. Using the bulked segregant analysis, the linkage analysis of the simple sequence repeat markers showed that the glabrous gene, gl-1, was flanked by GL311 and GL8 with the genetic distances of 0.19 and 0.92 cM on chromosome 5, respectively.展开更多
Arabidopsis trichomes are large branched single cells that protrude from the epidermis. The first mor- phological indication of trichome development is an increase in nuclear content resulting from an initial cycle of...Arabidopsis trichomes are large branched single cells that protrude from the epidermis. The first mor- phological indication of trichome development is an increase in nuclear content resulting from an initial cycle of endoreduplication. Our previous study has shown that the C2H2 zinc finger protein GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome initiation in the inflorescence organ and for trichome branching in response to gibberellic acid signaling, although GIS gene does not play a direct role in regulating trichome cell division. Here, we describe a novel role of GIS, controlling trichome cell division indirectly by interacting genetically with a key endoreduplication regulator SIAMESE (SIM). Our molecular and genetic studies have shown that GIS might indireclty control cell division and trichome branching by acting downstream of SIM. A loss of function mutation of SIM signficantly reduced the expression of GIS. Futhermore, the overexpression of GIS rescued the trichome cluster cell phenotypes of sim mutant. The gain or loss of function of GIS had no significant effect on the expression of SIM. These results suggest that GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM.展开更多
Glabrous rice exhibits glabrous leaves and hulls because neither of these structures have trichomes on their surfaces. Glabrous rice varieties have become an important germplasm resource in the rice industry because t...Glabrous rice exhibits glabrous leaves and hulls because neither of these structures have trichomes on their surfaces. Glabrous rice varieties have become an important germplasm resource in the rice industry because they have considerable packaging efficiency and can reduce skin itching and dust during harvesting, drying, and packing （Shim et al., 2012; Zhang et al., 2012）.展开更多
The yield potential of current rice varieties has reached a high plateau and fluc-tuated within a narrow range. Based on the theory and practice of rice breedingfor super-high-yield, we put forward a concept of "...The yield potential of current rice varieties has reached a high plateau and fluc-tuated within a narrow range. Based on the theory and practice of rice breedingfor super-high-yield, we put forward a concept of " Two high and one early",which means high biomass and high efficiency of light energy conversion, as wellas fast growing in the early growth stage. The agronomic characters of 15 U. S展开更多
Tubercles and spines on fruit peel are important commercial traits in cucumber(Cucumis sativus L.). From an ethyl methane sulfonate cucumber mutant library,we discovered a new glabrous mutant that bears no tubercle or...Tubercles and spines on fruit peel are important commercial traits in cucumber(Cucumis sativus L.). From an ethyl methane sulfonate cucumber mutant library,we discovered a new glabrous mutant that bears no tubercle or spine on fruit peel and fewer and smaller trichomes on the stem and leaves. The new locus is here designated as glabrous2(gl2). Genome sequencing of the mutant and linkage analysis revealed that a non-synonymous mutation in the Csa1G056960 gene rendered the gl2 phenotype. The mutated gene encodes a C-type lectin receptor-like tyrosine protein kinase. This study provides a novel allele for elucidating the genetic basis of wart and trichome development and a new tool for breeding glabrous cucumber varieties.展开更多
Transcription factors(TFs)play crucial roles in plant development and pathogen defense.However,plant viruses can exploit TFs to facilitate their infection or transmission.In this study,we confirmed theβC1 proteins,en...Transcription factors(TFs)play crucial roles in plant development and pathogen defense.However,plant viruses can exploit TFs to facilitate their infection or transmission.In this study,we confirmed theβC1 proteins,encoded by tobacco curly shoot virus(TbCSV)-and tomato yellow leaf curl China virus(TYLCCNV)-associated betasatellites,interacted with GLABROUS1 enhancer binding protein(GeBP)TFs from solanaceous plants including Nicotiana benthamiana,Solanum lycopersicum,S.tuberosum,and Capsicum annuum.Further analysis verified the nuclear localization,homodimerization,and DNA-binding ability of the GeBP TFs,along with its interaction withβC1 in the nucleus.PVX-mediated overexpression of NbGeBP showed no effect on the accumulation of viral and betasatellite DNAs in N.benthamiana plants after infection with TbCSV and its heterologous betasatellite,malvastrum yellow vein virus associated betasatellite(MaYVB),or its homologous betasatellite,TbCSB.However,both TbCSV and MaYVV caused a decrease in NbGeBP expression during the early stages of infection,regardless of the presence of homologous or heterologous betasatellites,implying that NbGeBP might play a role in virus infection.TbCSV/TbCSB and TYLCCNV/TYLCCNB infect many solanaceous plants,and solanaceous GeBP proteins interact withβC1 proteins from TbCSB and TYLCCNB.The yeast two-hybrid and bimoleccular fluorescence complementation assays showed that AtGeBP from Arabidopsis thaliana could not interact with TbCSBβC1,revealing that the GeBP-βC1 interactions might only exist in GeBP proteins from solanaceous plants.Importantly,theβC1 protein from MaYVB,which was almost not reported on natural infection in solanaceous plants,could not interact with GeBP,suggesting the potential roles of GeBP in monopartite begomovirus infection of solanaceous plants.展开更多
基金supported by the grants of the National High Technology Research and Development Program of China(Grant No.2006AA100101)the ‘948’program of China(Grant No.2006-G1)the National Technology Foundation from Ministry of Agriculture,China(Grant No.2006BAD01A01-1)
文摘Inheritance analysis and gene mapping of the glabrous trait were conducted using the crosses between a pubescent rice material Chuanxiang 29B （an aromatic elite maintainer line of hybrid rice）, and two glabrous rice materials, Lemont and R2 （R2 is a new restorer line of hybrid rice）. All F1 plants from the two crosses had pubescent leaves, confirming that the pubescence trait in Chuanxiang 29B is dominant over the glabrous trait. In F2 individuals, the segregation ratio of three pubescent to one glabrous plant suggests that a single glabrous gene was present in Lemont and R2. Using the bulked segregant analysis, the linkage analysis of the simple sequence repeat markers showed that the glabrous gene, gl-1, was flanked by GL311 and GL8 with the genetic distances of 0.19 and 0.92 cM on chromosome 5, respectively.
基金supported by the National Natural Science Foundation of China (Nos. 30970167 and 31228002)the Zhejiang Qianjiang Talent Program (No. 2010R10084)+1 种基金the Zhejiang Provincial Natural Science Foundation of China (No. Z31100041)the Zhejiang Province Foundation for Returned Scholars (No. 20100129), China
文摘Arabidopsis trichomes are large branched single cells that protrude from the epidermis. The first mor- phological indication of trichome development is an increase in nuclear content resulting from an initial cycle of endoreduplication. Our previous study has shown that the C2H2 zinc finger protein GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome initiation in the inflorescence organ and for trichome branching in response to gibberellic acid signaling, although GIS gene does not play a direct role in regulating trichome cell division. Here, we describe a novel role of GIS, controlling trichome cell division indirectly by interacting genetically with a key endoreduplication regulator SIAMESE (SIM). Our molecular and genetic studies have shown that GIS might indireclty control cell division and trichome branching by acting downstream of SIM. A loss of function mutation of SIM signficantly reduced the expression of GIS. Futhermore, the overexpression of GIS rescued the trichome cluster cell phenotypes of sim mutant. The gain or loss of function of GIS had no significant effect on the expression of SIM. These results suggest that GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM.
基金supported by the grants from the National Natural Science Foundation of China(Nos.31025017 and30971763)the National High-tech R&D Program of China(863 Program)(No.2012AA101101)
文摘Glabrous rice exhibits glabrous leaves and hulls because neither of these structures have trichomes on their surfaces. Glabrous rice varieties have become an important germplasm resource in the rice industry because they have considerable packaging efficiency and can reduce skin itching and dust during harvesting, drying, and packing （Shim et al., 2012; Zhang et al., 2012）.
文摘The yield potential of current rice varieties has reached a high plateau and fluc-tuated within a narrow range. Based on the theory and practice of rice breedingfor super-high-yield, we put forward a concept of " Two high and one early",which means high biomass and high efficiency of light energy conversion, as wellas fast growing in the early growth stage. The agronomic characters of 15 U. S
基金supported by funding from the National Program on Key Basic Research Projects in China(The 973 Program:2012CB113900)the National Natural Science Foundation of China(NSFC:31225025)+1 种基金Chinese Ministry of Finance(1251610601001)Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS)
文摘Tubercles and spines on fruit peel are important commercial traits in cucumber(Cucumis sativus L.). From an ethyl methane sulfonate cucumber mutant library,we discovered a new glabrous mutant that bears no tubercle or spine on fruit peel and fewer and smaller trichomes on the stem and leaves. The new locus is here designated as glabrous2(gl2). Genome sequencing of the mutant and linkage analysis revealed that a non-synonymous mutation in the Csa1G056960 gene rendered the gl2 phenotype. The mutated gene encodes a C-type lectin receptor-like tyrosine protein kinase. This study provides a novel allele for elucidating the genetic basis of wart and trichome development and a new tool for breeding glabrous cucumber varieties.
基金supported by the National Natural Science Foundation of China(32072380)Fundamental Research Funds for the Central Universities(SWUKT22058)+1 种基金Chongqing Postgraduate Research Innovation Project(CYB22140)Chongqing Municipal Training Program of Innovation and Entrepreneurship for Undergraduates(S202310635211 and X202310635107).
文摘Transcription factors(TFs)play crucial roles in plant development and pathogen defense.However,plant viruses can exploit TFs to facilitate their infection or transmission.In this study,we confirmed theβC1 proteins,encoded by tobacco curly shoot virus(TbCSV)-and tomato yellow leaf curl China virus(TYLCCNV)-associated betasatellites,interacted with GLABROUS1 enhancer binding protein(GeBP)TFs from solanaceous plants including Nicotiana benthamiana,Solanum lycopersicum,S.tuberosum,and Capsicum annuum.Further analysis verified the nuclear localization,homodimerization,and DNA-binding ability of the GeBP TFs,along with its interaction withβC1 in the nucleus.PVX-mediated overexpression of NbGeBP showed no effect on the accumulation of viral and betasatellite DNAs in N.benthamiana plants after infection with TbCSV and its heterologous betasatellite,malvastrum yellow vein virus associated betasatellite(MaYVB),or its homologous betasatellite,TbCSB.However,both TbCSV and MaYVV caused a decrease in NbGeBP expression during the early stages of infection,regardless of the presence of homologous or heterologous betasatellites,implying that NbGeBP might play a role in virus infection.TbCSV/TbCSB and TYLCCNV/TYLCCNB infect many solanaceous plants,and solanaceous GeBP proteins interact withβC1 proteins from TbCSB and TYLCCNB.The yeast two-hybrid and bimoleccular fluorescence complementation assays showed that AtGeBP from Arabidopsis thaliana could not interact with TbCSBβC1,revealing that the GeBP-βC1 interactions might only exist in GeBP proteins from solanaceous plants.Importantly,theβC1 protein from MaYVB,which was almost not reported on natural infection in solanaceous plants,could not interact with GeBP,suggesting the potential roles of GeBP in monopartite begomovirus infection of solanaceous plants.
