Plants have evolved several strategies to cope with the ever-changing environment.One example of this is given by seed germination,which must occur when environmental conditions are suitable for plant life.In the mode...Plants have evolved several strategies to cope with the ever-changing environment.One example of this is given by seed germination,which must occur when environmental conditions are suitable for plant life.In the model system Arabidopsis thaliana seed germination is induced by light;however,in nature,seeds of several plant species can germinate regardless of this stimulus.While the molecular mechanisms underlying light-induced seed germination are well understood,those governing germination in the dark are still vague,mostly due to the lack of suitable model systems.Here,we employ Cardamine hirsuta,a close relative of Ara-bidopsis,as a powerful model system to uncover the molecular mechanisms underlying light-independent germination.By comparing Cardamine and Arabidopsis,we show that maintenance of the pro-germination hormone gibberellin(GA)levels prompt Cardamine seeds to germinate under both dark and light conditions.Using genetic and molecular biology experiments,we show that the Cardamine DOF transcriptional repressor DOF AFFECTING GERMINATION 1(ChDAG1),homologous to the Arabidopsis transcription factor DAG1,is involved in this process functioning to mitigate GA levels by negatively regulating GA biosynthetic genes ChGA3OX1 and ChGA3OX2,independently of light conditions.We also demonstrate that this mechanism is likely conserved in other Brassicaceae species capable of germinating in dark conditions,such as Lepidium sativum and Camelina sativa.Our data support Cardamine as a new model system suitable for studying light-independent germination studies.Exploiting this system,we have also resolved a long-standing question about the mechanisms controlling light-independent germination in plants,opening new frontiers for future research.展开更多
Subject Code:C08With the support by the National Natural Science Foundation of China,a study by the research group led by Prof.Qi Hai(祁海)from the School of Medicine,Tsinghua University revealed a novel mechanism tha...Subject Code:C08With the support by the National Natural Science Foundation of China,a study by the research group led by Prof.Qi Hai(祁海)from the School of Medicine,Tsinghua University revealed a novel mechanism that regulates the germinal center reaction,aphysiological process that underlies high-quality展开更多
基金supported by the project SEMINE-POR FESR Lazio 2014-2020-Azione 1.2.1-approvato con Determinazione no.G08487 del 19/07/2020-pubblicato sul BURL N.93 del 23/07/2020-modificato con Determinazione no.G10624/2020-pubblicato sul BURL no.116 del 22/09/2020-Italy(to S.S.)the project TowArds Next GENeration Crops,reg.no.CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius-Czech Republic(to D.T.).
文摘Plants have evolved several strategies to cope with the ever-changing environment.One example of this is given by seed germination,which must occur when environmental conditions are suitable for plant life.In the model system Arabidopsis thaliana seed germination is induced by light;however,in nature,seeds of several plant species can germinate regardless of this stimulus.While the molecular mechanisms underlying light-induced seed germination are well understood,those governing germination in the dark are still vague,mostly due to the lack of suitable model systems.Here,we employ Cardamine hirsuta,a close relative of Ara-bidopsis,as a powerful model system to uncover the molecular mechanisms underlying light-independent germination.By comparing Cardamine and Arabidopsis,we show that maintenance of the pro-germination hormone gibberellin(GA)levels prompt Cardamine seeds to germinate under both dark and light conditions.Using genetic and molecular biology experiments,we show that the Cardamine DOF transcriptional repressor DOF AFFECTING GERMINATION 1(ChDAG1),homologous to the Arabidopsis transcription factor DAG1,is involved in this process functioning to mitigate GA levels by negatively regulating GA biosynthetic genes ChGA3OX1 and ChGA3OX2,independently of light conditions.We also demonstrate that this mechanism is likely conserved in other Brassicaceae species capable of germinating in dark conditions,such as Lepidium sativum and Camelina sativa.Our data support Cardamine as a new model system suitable for studying light-independent germination studies.Exploiting this system,we have also resolved a long-standing question about the mechanisms controlling light-independent germination in plants,opening new frontiers for future research.
文摘Subject Code:C08With the support by the National Natural Science Foundation of China,a study by the research group led by Prof.Qi Hai(祁海)from the School of Medicine,Tsinghua University revealed a novel mechanism that regulates the germinal center reaction,aphysiological process that underlies high-quality
