Through investigating Gentianaceae plant resources in Guizhou Province, collecting and identifying specimens, consulting relevant records and documents, Gentianaceae plant resources in Guizhou Province and their ornam...Through investigating Gentianaceae plant resources in Guizhou Province, collecting and identifying specimens, consulting relevant records and documents, Gentianaceae plant resources in Guizhou Province and their ornamental characteristics have been elaborated, and the ornamental species of Gentianaceae plants in this region were introduced.展开更多
Establishing how lineages with similar traits are phylogenetically related remains critical for understanding the origin of biodiversity on Earth.Floral traits in plants are widely used to explore phylogenetic relatio...Establishing how lineages with similar traits are phylogenetically related remains critical for understanding the origin of biodiversity on Earth.Floral traits in plants are widely used to explore phylogenetic relationships and to delineate taxonomic groups.The subtribe Swertiinae(Gentianaceae)comprises more than 350 species with high floral diversity ranging from rotate to tubular corollas and possessing diverse nectaries.Here we performed phylogenetic analysis of 60 species from all 15 genera of the subtribe Swertiinae sensu Ho and Liu,representing the range of floral diversity,using data from the nuclear and plastid genomes.Extensive topological conflicts were present between the nuclear and plastome trees.Three of the 15 genera represented by multiple species are polyphyletic in both trees.Key floral traits including corolla type,absence or presence of lobe scales,nectary type,nectary position,and stigma type are randomly distributed in the nuclear and plastome trees without phylogenetic correlation.We also revealed the likely ancient hybrid origin of one large clade comprising 10 genera with diverse floral traits.These results highlight the complex evolutionary history of this subtribe.The phylogenies constructed here provide a basic framework for further exploring the ecological and genetic mechanisms underlying both species diversification and floral diversity.展开更多
Gentianopsis barbata(G.barbata)represents a significant plant species with considerable ornamental and medicinal value in China.This investigation sought to elucidate the primary constituents within the plant and inve...Gentianopsis barbata(G.barbata)represents a significant plant species with considerable ornamental and medicinal value in China.This investigation sought to elucidate the primary constituents within the plant and investigate their pharmacological properties.Fifty triterpenoids(1-50),including nine previously undescribed compounds(1,2,7,10,20,28,29,37,and 41)were isolated and characterized from the whole plants of G.barbata.Notably,compounds 1 and 2 exhibited the novel 3,4;9,10-diseco-24-homo-cycloartane triterpenoid skeleton.The isolated triterpenoids demonstrated substantial anti-inflammatory activity through inhibition of tumor necrosis factorα(TNF-α)and interleukin-6(IL-6)cytokine secretion in LPS-induced RAW264.7 macrophages,and hepatoprotective effects by preventing tertbutyl hydroperoxide(t-BHP)-induced oxidative injury in HepG2 cells.These results demonstrate both the presence of diverse triterpenoids in G.barbata and their therapeutic potential for inflammatory and hepatic conditions,providing scientific evidence supporting the clinical application of this traditional Mongolian medicinal plant.展开更多
From the CHCl3 part of Swertia decora Franch., a traditional medicinal herb of China, two new xanthones, swertiadecoraxanthone-I and swertiadecoraxanthone-II along with four known compounds have been isolated and iden...From the CHCl3 part of Swertia decora Franch., a traditional medicinal herb of China, two new xanthones, swertiadecoraxanthone-I and swertiadecoraxanthone-II along with four known compounds have been isolated and identified on the basis of spectral analysis and chemical evidence.展开更多
Chemical study on the roots of Gentiana crassicaulis Duthie ex Burk(Gentianaceae)afforded 15 compounds,including two new iridoid glycosides,qinjiaosides B(1)and C(2).Their structures were elucidated by spectroscopic m...Chemical study on the roots of Gentiana crassicaulis Duthie ex Burk(Gentianaceae)afforded 15 compounds,including two new iridoid glycosides,qinjiaosides B(1)and C(2).Their structures were elucidated by spectroscopic methods and chemical evidence.The isolated iridoid glycosides 1,4-6 and 8-11 were tested for their anti-inflammatory activity by the inhibitory effects on LPS-induced NO and TNF-αproduction in macrophage RAW264.7 cells.All of them showed inhibitory effects on inflammatory mediators NO at a concentration of 15μM,while 5 and 9 displayed the most potential inhibitory effects on TNF-awith IC50 of 0.06 and 0.05μM,respectively.The structure-activity relationships(SARs)of these iridoid derivatives were discussed.展开更多
AIM: To study the chemical constituents of Lomatogonium carinthiacum(Wulfen) Rchb. METHOD: The CHCl3-soluble fraction was separated by chromatography and the structures of the new compounds were elucidated by spectral...AIM: To study the chemical constituents of Lomatogonium carinthiacum(Wulfen) Rchb. METHOD: The CHCl3-soluble fraction was separated by chromatography and the structures of the new compounds were elucidated by spectral experiments. RESULTS: Two new xanthones, 1, 8-dihydroxy-4, 5-dimethoxy-6, 7-methylenedioxyxanthone(1), 1, 4, 8-trimethoxyxanthone-6-O-β-D-glucoronyl-(1→6)O-β-D-glucoside(2) were isolated from the whole plant of Lomatogonium carinthiacum. CONCLUSION: Compounds 1 and 2 are new natural products.展开更多
A novel monoterpene glycoside, mileenside, was isolated from Swertia mileensis by repeated normal and reverse phase silica gel chromatography and its structure was elucidated mainly based on spectral evidence.
Objective To study the chemical constituents of Swertia cincta. Methods Preparative liquid chromatography was employed. The structures of the compounds were elucidated by spectroscopic analysis. isolated from S. cinct...Objective To study the chemical constituents of Swertia cincta. Methods Preparative liquid chromatography was employed. The structures of the compounds were elucidated by spectroscopic analysis. isolated from S. cincta and identified as Results Three secoiridoid glycosides were 8-methoxyl-eustomorusside （1）, secoiridoids eustomorusside （2）, and eustomoside （3）. Conclusion Compound 1 is a new secoiridoid glycoside. Compounds 2 and 3 are isolated from this plant for the first time.展开更多
Objective To search for the potential medicinal plant part of Gentiana macrophyllabased on changes of secondary metabolites and trace elements in the flowers of G.macrophylla.Methods HPLC was used to detect the change...Objective To search for the potential medicinal plant part of Gentiana macrophyllabased on changes of secondary metabolites and trace elements in the flowers of G.macrophylla.Methods HPLC was used to detect the changes of the active constituents（longanic acid,sweroside,gentiopicroside,and swertiamarin）and ICP-AES was used for mineral nutrients in G.macrophylla during flower development.And soluble sugar,starch,crude protein,hemicelluloses,cellulose,and lignin were determined.Results Biomass of flower in full bloom（D2）phase was considerable during flower development,in which the contents of longanic acid and gentiopicroside were at the highest levels with 2.65 and 2.88 times higher than those recorded in Chinese Pharmacopoeia 2010,sweroside and swertiamarin in the flowers were reaching 6.06 and 1.25 times higher than those in roots.Florescence is the most valuable stage during flower development.The concentration of Fe,Mg,K,P,and B was higher in the flowers than that in roots.The accumulation of active constituents in the plant was influenced by the contents of metabolically linked carbon and nitrogen compounds.Conclusion The secondary metabolites,mineral nutrients,and physicochemical indicators are tightly regulated by flower organ development,D2 is an important stage for both biomass and extraction of active constituents such as longanic acid.The flowers of G.macrophylla could be used as a potential medicinal plant part for longanic acid at a high level.展开更多
文摘Through investigating Gentianaceae plant resources in Guizhou Province, collecting and identifying specimens, consulting relevant records and documents, Gentianaceae plant resources in Guizhou Province and their ornamental characteristics have been elaborated, and the ornamental species of Gentianaceae plants in this region were introduced.
基金supported by the Natural Science Foundation of China(32170221)the Strategic Priority Research Program of Chinese Academy of Sciences(XDB31000000)Fundamental Research Funds for the Central Universities(YJ201936,SCU2019D013 and 2020SCUNL20)。
文摘Establishing how lineages with similar traits are phylogenetically related remains critical for understanding the origin of biodiversity on Earth.Floral traits in plants are widely used to explore phylogenetic relationships and to delineate taxonomic groups.The subtribe Swertiinae(Gentianaceae)comprises more than 350 species with high floral diversity ranging from rotate to tubular corollas and possessing diverse nectaries.Here we performed phylogenetic analysis of 60 species from all 15 genera of the subtribe Swertiinae sensu Ho and Liu,representing the range of floral diversity,using data from the nuclear and plastid genomes.Extensive topological conflicts were present between the nuclear and plastome trees.Three of the 15 genera represented by multiple species are polyphyletic in both trees.Key floral traits including corolla type,absence or presence of lobe scales,nectary type,nectary position,and stigma type are randomly distributed in the nuclear and plastome trees without phylogenetic correlation.We also revealed the likely ancient hybrid origin of one large clade comprising 10 genera with diverse floral traits.These results highlight the complex evolutionary history of this subtribe.The phylogenies constructed here provide a basic framework for further exploring the ecological and genetic mechanisms underlying both species diversification and floral diversity.
基金supported by the National Natural Science Foundation of China(Nos.21937006,U23A20510,82204245,82222072,and U24A20806)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB1230000)the Research Project of Yunnan Province(Nos.202401BC070016 and 202402AA310047)。
文摘Gentianopsis barbata(G.barbata)represents a significant plant species with considerable ornamental and medicinal value in China.This investigation sought to elucidate the primary constituents within the plant and investigate their pharmacological properties.Fifty triterpenoids(1-50),including nine previously undescribed compounds(1,2,7,10,20,28,29,37,and 41)were isolated and characterized from the whole plants of G.barbata.Notably,compounds 1 and 2 exhibited the novel 3,4;9,10-diseco-24-homo-cycloartane triterpenoid skeleton.The isolated triterpenoids demonstrated substantial anti-inflammatory activity through inhibition of tumor necrosis factorα(TNF-α)and interleukin-6(IL-6)cytokine secretion in LPS-induced RAW264.7 macrophages,and hepatoprotective effects by preventing tertbutyl hydroperoxide(t-BHP)-induced oxidative injury in HepG2 cells.These results demonstrate both the presence of diverse triterpenoids in G.barbata and their therapeutic potential for inflammatory and hepatic conditions,providing scientific evidence supporting the clinical application of this traditional Mongolian medicinal plant.
基金This work was supported in part by Shanghai Municipal Educational Committee (grants S970204).
文摘From the CHCl3 part of Swertia decora Franch., a traditional medicinal herb of China, two new xanthones, swertiadecoraxanthone-I and swertiadecoraxanthone-II along with four known compounds have been isolated and identified on the basis of spectral analysis and chemical evidence.
基金This work was supported by the Science&Technology Planning Project of Yunnan Province(2010CD106)the 973 Program of Ministry of Science&Technology of China(2011CB915503)+2 种基金the State Key Laboratory of Phytochemistry and Plant Resources in West China,Chinese Academy of Sciences(CAS)(P2010-ZZ03)the Fourteenth Candidates of the Young Academic Leaders of Yunnan Province(Min XU,2011CI044)the West Light program of CAS.
文摘Chemical study on the roots of Gentiana crassicaulis Duthie ex Burk(Gentianaceae)afforded 15 compounds,including two new iridoid glycosides,qinjiaosides B(1)and C(2).Their structures were elucidated by spectroscopic methods and chemical evidence.The isolated iridoid glycosides 1,4-6 and 8-11 were tested for their anti-inflammatory activity by the inhibitory effects on LPS-induced NO and TNF-αproduction in macrophage RAW264.7 cells.All of them showed inhibitory effects on inflammatory mediators NO at a concentration of 15μM,while 5 and 9 displayed the most potential inhibitory effects on TNF-awith IC50 of 0.06 and 0.05μM,respectively.The structure-activity relationships(SARs)of these iridoid derivatives were discussed.
基金supported by the National Natural Science Foundation of China(No.81360673)
文摘AIM: To study the chemical constituents of Lomatogonium carinthiacum(Wulfen) Rchb. METHOD: The CHCl3-soluble fraction was separated by chromatography and the structures of the new compounds were elucidated by spectral experiments. RESULTS: Two new xanthones, 1, 8-dihydroxy-4, 5-dimethoxy-6, 7-methylenedioxyxanthone(1), 1, 4, 8-trimethoxyxanthone-6-O-β-D-glucoronyl-(1→6)O-β-D-glucoside(2) were isolated from the whole plant of Lomatogonium carinthiacum. CONCLUSION: Compounds 1 and 2 are new natural products.
文摘A novel monoterpene glycoside, mileenside, was isolated from Swertia mileensis by repeated normal and reverse phase silica gel chromatography and its structure was elucidated mainly based on spectral evidence.
基金NSFC(NO.21262048)Youth Foundation of Lincang Teachers’College(LCSZL201204)
文摘Objective To study the chemical constituents of Swertia cincta. Methods Preparative liquid chromatography was employed. The structures of the compounds were elucidated by spectroscopic analysis. isolated from S. cincta and identified as Results Three secoiridoid glycosides were 8-methoxyl-eustomorusside （1）, secoiridoids eustomorusside （2）, and eustomoside （3）. Conclusion Compound 1 is a new secoiridoid glycoside. Compounds 2 and 3 are isolated from this plant for the first time.
基金Major National Science and Technology Program(2009ZX09308)
文摘Objective To search for the potential medicinal plant part of Gentiana macrophyllabased on changes of secondary metabolites and trace elements in the flowers of G.macrophylla.Methods HPLC was used to detect the changes of the active constituents（longanic acid,sweroside,gentiopicroside,and swertiamarin）and ICP-AES was used for mineral nutrients in G.macrophylla during flower development.And soluble sugar,starch,crude protein,hemicelluloses,cellulose,and lignin were determined.Results Biomass of flower in full bloom（D2）phase was considerable during flower development,in which the contents of longanic acid and gentiopicroside were at the highest levels with 2.65 and 2.88 times higher than those recorded in Chinese Pharmacopoeia 2010,sweroside and swertiamarin in the flowers were reaching 6.06 and 1.25 times higher than those in roots.Florescence is the most valuable stage during flower development.The concentration of Fe,Mg,K,P,and B was higher in the flowers than that in roots.The accumulation of active constituents in the plant was influenced by the contents of metabolically linked carbon and nitrogen compounds.Conclusion The secondary metabolites,mineral nutrients,and physicochemical indicators are tightly regulated by flower organ development,D2 is an important stage for both biomass and extraction of active constituents such as longanic acid.The flowers of G.macrophylla could be used as a potential medicinal plant part for longanic acid at a high level.
