The Bakerwal breed of goat in Kashmir valley is a good meat purpose breed of goat. That attains an appreciable body weight under a low input production system. As these goats are mainly reared by Gujjar and Bakarwal t...The Bakerwal breed of goat in Kashmir valley is a good meat purpose breed of goat. That attains an appreciable body weight under a low input production system. As these goats are mainly reared by Gujjar and Bakarwal tribes of the J & K state, so they usually are fed with the weeds, herbs, shrubs and grasses of pastures that will otherwise go waste. These goats constitute the main livestock wealth. With the good productive and reproductive potential, it makes these animals an important animal protein source for developing countries like India. The myostatin gene (GDF8) is important in the physiology of stock animals because its product produces a direct effect on muscle development and consequently also on meat production. The myostatin sequence is known in several mammalian species and shows a high degree of amino acid sequence conservation, although several alterations in the intron and exon regions have been identified. The objective of our work is to characterize the myostatin coding regions using gene sequencing and polymerase chain reaction methods of Capra hircus (Bakerwal breed) and to compare them with the Ovis aries and other livestock species of animal, looking for variations in nucleotide and protein sequences. As mutations in the myostatin gene can inactivate its expression and result in a non-functional protein, which leads to increase in muscle growth in many species. In this way, we are able to identify 3 alterations on the presumed myostatin protein sequence as compared to non double-muscled ovine sequences.展开更多
目的:通过肌肉注射异体脂肪间充质干细胞(ASCs),观察其对一次性离心运动后不同时间点大鼠骨骼肌调节因子的影响,探讨ASCs注射对离心运动后骨骼肌损伤修复的机制。方法:8周龄雄性SD大鼠一次性离心运动后,在左腿腓肠肌注射生理盐水(PBS),...目的:通过肌肉注射异体脂肪间充质干细胞(ASCs),观察其对一次性离心运动后不同时间点大鼠骨骼肌调节因子的影响,探讨ASCs注射对离心运动后骨骼肌损伤修复的机制。方法:8周龄雄性SD大鼠一次性离心运动后,在左腿腓肠肌注射生理盐水(PBS),右腿腓肠肌注射ASCs,然后,随机分为运动后1天组(D1)、运动后3天组(D3)、运动后7天组(W1)和运动后14天组(W2)。透射电镜观察骨骼肌的超微结构变化;测定血清IGF1、GDF8的含量及骨骼肌调节基因的表达。结果:与PBS相比,ASCs注射明显促进肌纤维的修复。与D1组比较,W1组血清IGF1水平显著降低(P<0.05),GDF8水平显著降低(P<0.05),W2组血清GDF8水平显著升高(P<0.05)。与PBS组相比,ASCs组IGF1 m RNA在W2显著升高(P<0.05);GDF8m RNA在D3显著降低(P<0.05),在W2极显著降低(P<0.01);Atrogin1 m RNA在D3显著降低(P<0.05),在W1时间点极显著降低(P<0.01)。PBS组IGF1 m RNA与血清IGF1含量、GDF8m RNA与血清GDF8含量均显著性正相关(P<0.05);骨骼肌总的PCNA m RNA与总的IGF1m RNA显著性正相关(P<0.05)。结论:离心运动后,肌注异体ASCs可以增加骨骼肌IGF1m RNA的表达,降低GDF8和Atrogin1的m RNA表达。肌注异体ASCs可能不影响细胞因子IGF1和GDF8的分泌,而是通过影响局部基因表达来改善离心运动后肌肉的再生修复。展开更多
文摘The Bakerwal breed of goat in Kashmir valley is a good meat purpose breed of goat. That attains an appreciable body weight under a low input production system. As these goats are mainly reared by Gujjar and Bakarwal tribes of the J & K state, so they usually are fed with the weeds, herbs, shrubs and grasses of pastures that will otherwise go waste. These goats constitute the main livestock wealth. With the good productive and reproductive potential, it makes these animals an important animal protein source for developing countries like India. The myostatin gene (GDF8) is important in the physiology of stock animals because its product produces a direct effect on muscle development and consequently also on meat production. The myostatin sequence is known in several mammalian species and shows a high degree of amino acid sequence conservation, although several alterations in the intron and exon regions have been identified. The objective of our work is to characterize the myostatin coding regions using gene sequencing and polymerase chain reaction methods of Capra hircus (Bakerwal breed) and to compare them with the Ovis aries and other livestock species of animal, looking for variations in nucleotide and protein sequences. As mutations in the myostatin gene can inactivate its expression and result in a non-functional protein, which leads to increase in muscle growth in many species. In this way, we are able to identify 3 alterations on the presumed myostatin protein sequence as compared to non double-muscled ovine sequences.
文摘目的:通过肌肉注射异体脂肪间充质干细胞(ASCs),观察其对一次性离心运动后不同时间点大鼠骨骼肌调节因子的影响,探讨ASCs注射对离心运动后骨骼肌损伤修复的机制。方法:8周龄雄性SD大鼠一次性离心运动后,在左腿腓肠肌注射生理盐水(PBS),右腿腓肠肌注射ASCs,然后,随机分为运动后1天组(D1)、运动后3天组(D3)、运动后7天组(W1)和运动后14天组(W2)。透射电镜观察骨骼肌的超微结构变化;测定血清IGF1、GDF8的含量及骨骼肌调节基因的表达。结果:与PBS相比,ASCs注射明显促进肌纤维的修复。与D1组比较,W1组血清IGF1水平显著降低(P<0.05),GDF8水平显著降低(P<0.05),W2组血清GDF8水平显著升高(P<0.05)。与PBS组相比,ASCs组IGF1 m RNA在W2显著升高(P<0.05);GDF8m RNA在D3显著降低(P<0.05),在W2极显著降低(P<0.01);Atrogin1 m RNA在D3显著降低(P<0.05),在W1时间点极显著降低(P<0.01)。PBS组IGF1 m RNA与血清IGF1含量、GDF8m RNA与血清GDF8含量均显著性正相关(P<0.05);骨骼肌总的PCNA m RNA与总的IGF1m RNA显著性正相关(P<0.05)。结论:离心运动后,肌注异体ASCs可以增加骨骼肌IGF1m RNA的表达,降低GDF8和Atrogin1的m RNA表达。肌注异体ASCs可能不影响细胞因子IGF1和GDF8的分泌,而是通过影响局部基因表达来改善离心运动后肌肉的再生修复。
