There was little research on the changes and interconversion of organic compounds in the intermediate liquid-phase productsin the hydrogen production by anaerobic fermentation of coal.The types and concentrations of t...There was little research on the changes and interconversion of organic compounds in the intermediate liquid-phase productsin the hydrogen production by anaerobic fermentation of coal.The types and concentrations of the intermediate liquid-phase products are not only related to the efficiency of coal conversion into biohydrogen,but one of the important contents of study on the generation mechanism of biohydrogen from coal.In order to study the change of key liquid phase products in the process of bio-hydrogen production from coal,the simulation experiments of bio-hydrogen production were conducted under suitable environmental conditions using lignite from Daliuta,Shaanxi province.The hydrogen concentration and key liquid phase products were measured using gas chromatography and GC-MS,respectively,to reveal its change.The results show that:(1)there is correspondence between the key liquid phase products and hydrogen yield.The saturated alkanes and cyclic hydrocarbons show a trend of first increasing,then decreasing,and finally increasing,but the changes of proteinaceous materials are just the opposite,which is correspondent to the initial stage,peak stage and terminal stage of hydrogen production,respectively.(2)The short-chain volatile fatty acids are accumulated gradually in the process of hydrogen production,and the short-chain acids of aromatic show a trend of first increasing,then decreasing,increase again and finally decreasing,which is correspondent to the different biochemical reactions,such as the release of small molecules,the utilization of microorganisms,hydrocarbon oxidation and the redegradation of macromolecule.(3)The lipids,aldehydes and ketones are the parent substances of acid production,and the changes are related to the increased acids to a certain extent.This research not only reveals the biochemical effects between gas and liquid phases,but further enriches the theory of bio-hydrogen production from coal.展开更多
Preeclampsia is characterized by new onset of hypertension and proteinuria after 20 weeks’gestation and is a leading cause of maternal and neonatal morbidity and mortality.The pathogenesis of preeclampsia is often as...Preeclampsia is characterized by new onset of hypertension and proteinuria after 20 weeks’gestation and is a leading cause of maternal and neonatal morbidity and mortality.The pathogenesis of preeclampsia is often associated with aberrant trophoblast function that leads to shallow placental implantation.However,the exact underlying mechanisms remain unclear.Placental LncZBTB39-1:2 expression level was investigated in 20 healthy placentae and 20 placentae with preeclampsia using qRT-PCR,and the metabolic profile of trophoblasts overexpressing LncZBTB39-1:2 in vitro was analysed using gas chromatography-mass spectrometry(GCeMS).In this study,we found that the expression of LncZBTB39-1:2 was significantly higher in preeclamptic placentae than in healthy placentae.Our metabolomics results have shown that tricarboxylic acid cycle intermediates and metabolites related to carbohydrate metabolism were decreased with the overexpression of LncZBTB39-1:2 in HTR8/SVneo cells.These findings were validated by detecting a lower level of intracellular ATP in HTR8/Vneo cells.Furthermore,the migration of HTR8/SVneo cells was compromised when cells were transfected with a plasmid encompassing LncZBTB39-1:2 overexpression.From these results,we conclude that abnormal levels of LncZBTB39-1:2 expression might lead to aberrant conditions in HTR-8/SVneo trophoblast cells.Aberrant conditions might be associated with dysregulated trophoblast migration and subsequent failure of uterine spiral artery remodelling,a pathogenesis recognised as a contributing factor in the aetiology of preeclampsia.展开更多
基金Project supported by the National Natural Science Foundation of China“Response Mechanism of Trace Elements to Coal Methane Generation”(No.41502158)Henan Science and Technology Key Project“Key Technologies and Applications of Coal and Straw Synergistic Conversion of Biomethane”(No.:182102310845).
文摘There was little research on the changes and interconversion of organic compounds in the intermediate liquid-phase productsin the hydrogen production by anaerobic fermentation of coal.The types and concentrations of the intermediate liquid-phase products are not only related to the efficiency of coal conversion into biohydrogen,but one of the important contents of study on the generation mechanism of biohydrogen from coal.In order to study the change of key liquid phase products in the process of bio-hydrogen production from coal,the simulation experiments of bio-hydrogen production were conducted under suitable environmental conditions using lignite from Daliuta,Shaanxi province.The hydrogen concentration and key liquid phase products were measured using gas chromatography and GC-MS,respectively,to reveal its change.The results show that:(1)there is correspondence between the key liquid phase products and hydrogen yield.The saturated alkanes and cyclic hydrocarbons show a trend of first increasing,then decreasing,and finally increasing,but the changes of proteinaceous materials are just the opposite,which is correspondent to the initial stage,peak stage and terminal stage of hydrogen production,respectively.(2)The short-chain volatile fatty acids are accumulated gradually in the process of hydrogen production,and the short-chain acids of aromatic show a trend of first increasing,then decreasing,increase again and finally decreasing,which is correspondent to the different biochemical reactions,such as the release of small molecules,the utilization of microorganisms,hydrocarbon oxidation and the redegradation of macromolecule.(3)The lipids,aldehydes and ketones are the parent substances of acid production,and the changes are related to the increased acids to a certain extent.This research not only reveals the biochemical effects between gas and liquid phases,but further enriches the theory of bio-hydrogen production from coal.
基金financed by the 111 Project(Yuwaizhuan(2016)32),China.
文摘Preeclampsia is characterized by new onset of hypertension and proteinuria after 20 weeks’gestation and is a leading cause of maternal and neonatal morbidity and mortality.The pathogenesis of preeclampsia is often associated with aberrant trophoblast function that leads to shallow placental implantation.However,the exact underlying mechanisms remain unclear.Placental LncZBTB39-1:2 expression level was investigated in 20 healthy placentae and 20 placentae with preeclampsia using qRT-PCR,and the metabolic profile of trophoblasts overexpressing LncZBTB39-1:2 in vitro was analysed using gas chromatography-mass spectrometry(GCeMS).In this study,we found that the expression of LncZBTB39-1:2 was significantly higher in preeclamptic placentae than in healthy placentae.Our metabolomics results have shown that tricarboxylic acid cycle intermediates and metabolites related to carbohydrate metabolism were decreased with the overexpression of LncZBTB39-1:2 in HTR8/SVneo cells.These findings were validated by detecting a lower level of intracellular ATP in HTR8/Vneo cells.Furthermore,the migration of HTR8/SVneo cells was compromised when cells were transfected with a plasmid encompassing LncZBTB39-1:2 overexpression.From these results,we conclude that abnormal levels of LncZBTB39-1:2 expression might lead to aberrant conditions in HTR-8/SVneo trophoblast cells.Aberrant conditions might be associated with dysregulated trophoblast migration and subsequent failure of uterine spiral artery remodelling,a pathogenesis recognised as a contributing factor in the aetiology of preeclampsia.
