为实现复杂食品中多种典型营养成分的高效识别与含量差异分析,提升营养成分检测的准确性与通量效率,本文采用超高效液相色谱-四极杆-飞行时间质谱联用(Ultra High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Sp...为实现复杂食品中多种典型营养成分的高效识别与含量差异分析,提升营养成分检测的准确性与通量效率,本文采用超高效液相色谱-四极杆-飞行时间质谱联用(Ultra High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry,UHPLC-Q-TOFMS)技术测定食品中维生素C、苯丙氨酸、槲皮素等具有代表性的营养物质,并在6类食品样品中开展实际应用。结果表明,不同类型食品在3类目标成分的含量分布上存在显著差异。主成分分析与聚类分析揭示了食品类别间的营养结构特征:果蔬类样品富含维生素C与槲皮素,表现出典型的抗氧化优势;谷物与坚果类以苯丙氨酸为主要成分,反映出植物蛋白代谢物的积累特征;乳制品与肉制品则因多酚类未检出,聚为独立分支,营养构成较为单一。本研究可为食品营养评价、标签构建及功能成分筛选提供有效的数据支撑。展开更多
This paper reports for the first time an experiment designed in which synthesized α cyano ferulic acid used as a new matrix for the analysis of cytochrome c, trypsin, bovine serum albumin, β cyclodextrin, dextrans a...This paper reports for the first time an experiment designed in which synthesized α cyano ferulic acid used as a new matrix for the analysis of cytochrome c, trypsin, bovine serum albumin, β cyclodextrin, dextrans and DNA d(T) 18 . The results demonstrated that α cyano ferulic acid used as a new matrix could effectively desorb and ionize different kinds of samples, such as proteins and peptides, oligosaccharides, polysaccharides and oligonucleotides. In analysis of dextrans the new matrix gave better results when comparing with the normally used DHB. In analysis of DNA d(T) 18 , the sample could be desorbed and ionized more efficiently even without sample purification, which is normally needed when using 3 HPA as a matrix.展开更多
建立了全二维气相色谱-飞行时间质谱(GC×GC-TOF MS)分析卷烟主流烟气中中性化学成分的方法。以较长的弱极性柱HP-5MS(50 m×0.2 mm i.d.×0.33μm)作为第一维柱,较短的薄液膜中等极性柱DB-17MS(1.7 m×0.1 mm i.d....建立了全二维气相色谱-飞行时间质谱(GC×GC-TOF MS)分析卷烟主流烟气中中性化学成分的方法。以较长的弱极性柱HP-5MS(50 m×0.2 mm i.d.×0.33μm)作为第一维柱,较短的薄液膜中等极性柱DB-17MS(1.7 m×0.1 mm i.d.×0.1μm)作为第二维柱,对优质烟叶单料卷烟烟气的中性成分进行定性分析,经过人工纠错等分析初步鉴定出匹配度大于700的1 464种成分,重点讨论了中性香味羰基化合物全二维点阵的谱图特征,为烟气和复杂体系的深入研究提供了方法学基础。展开更多
文摘为实现复杂食品中多种典型营养成分的高效识别与含量差异分析,提升营养成分检测的准确性与通量效率,本文采用超高效液相色谱-四极杆-飞行时间质谱联用(Ultra High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry,UHPLC-Q-TOFMS)技术测定食品中维生素C、苯丙氨酸、槲皮素等具有代表性的营养物质,并在6类食品样品中开展实际应用。结果表明,不同类型食品在3类目标成分的含量分布上存在显著差异。主成分分析与聚类分析揭示了食品类别间的营养结构特征:果蔬类样品富含维生素C与槲皮素,表现出典型的抗氧化优势;谷物与坚果类以苯丙氨酸为主要成分,反映出植物蛋白代谢物的积累特征;乳制品与肉制品则因多酚类未检出,聚为独立分支,营养构成较为单一。本研究可为食品营养评价、标签构建及功能成分筛选提供有效的数据支撑。
文摘This paper reports for the first time an experiment designed in which synthesized α cyano ferulic acid used as a new matrix for the analysis of cytochrome c, trypsin, bovine serum albumin, β cyclodextrin, dextrans and DNA d(T) 18 . The results demonstrated that α cyano ferulic acid used as a new matrix could effectively desorb and ionize different kinds of samples, such as proteins and peptides, oligosaccharides, polysaccharides and oligonucleotides. In analysis of dextrans the new matrix gave better results when comparing with the normally used DHB. In analysis of DNA d(T) 18 , the sample could be desorbed and ionized more efficiently even without sample purification, which is normally needed when using 3 HPA as a matrix.
文摘建立了全二维气相色谱-飞行时间质谱(GC×GC-TOF MS)分析卷烟主流烟气中中性化学成分的方法。以较长的弱极性柱HP-5MS(50 m×0.2 mm i.d.×0.33μm)作为第一维柱,较短的薄液膜中等极性柱DB-17MS(1.7 m×0.1 mm i.d.×0.1μm)作为第二维柱,对优质烟叶单料卷烟烟气的中性成分进行定性分析,经过人工纠错等分析初步鉴定出匹配度大于700的1 464种成分,重点讨论了中性香味羰基化合物全二维点阵的谱图特征,为烟气和复杂体系的深入研究提供了方法学基础。
