Background:Cyperi Rhizoma,derived from Cyperus rotundus L.,is a widely used medicinal herb in traditional Chinese medicine(TCM),with Shandong Province recognized as its geo-authentic habitat.However,the quality of Cyp...Background:Cyperi Rhizoma,derived from Cyperus rotundus L.,is a widely used medicinal herb in traditional Chinese medicine(TCM),with Shandong Province recognized as its geo-authentic habitat.However,the quality of Cyperi Rhizoma varies significantly across different regions,potentially influencing its therapeutic efficacy.This study investigates the influence of geographic origin on the chemical composition and overall quality of Cyperi Rhizoma.Methods:A comprehensive approach,including traditional quality assessment,GC-MS(g as c hromatography-m ass s pectrometry),RP-HPLC(r everse p hase h igh-p erformance l iquid c hromatography),and FT-IR(f ourier t ransform i nfrared s pectroscopy)techniques,was employed to analyze Cyperi Rhizoma samples from Shandong Province.These methods examined the physical appearance,chemical profile,and content variations,particularly focusing onα-cyperone.Results:Traditional quality assessments revealed noticeable differences in the external characteristics of the samples.GC-MS analysis identified a variety of unique chemical constituents,while RP-HPLC and FT-IR showed significant variations inα-cyperone content,with higher levels found in Shandong samples.Conclusion:These results demonstrate that geographic origin is a critical determinant of Cyperi Rhizoma quality,with Shandong specimens exhibiting superiorα-cyperone levels and characteristic phytochemical profiles.This validates the geo-authenticity concept in TCM and provides actionable data for developing evidence-based quality standards,suggesting that provenance should be prioritized in medicinal material selection and pharmacopeial specifications.展开更多
A trace analytical method based on solid-phase extraction gas chromatography-tandem mass spectrometry(SPE–GC–MS/MS)was developed for the rapid detection of 256 semi-volatile organic compounds(SVOCs),including 25 pol...A trace analytical method based on solid-phase extraction gas chromatography-tandem mass spectrometry(SPE–GC–MS/MS)was developed for the rapid detection of 256 semi-volatile organic compounds(SVOCs),including 25 polycyclic aromatic hydrocarbons(PAHs),70 polychlorinated biphenyls(PCBs),123 pesticides,20 phthalate esters(PAEs),4 organophosphate esters(OPEs),9 synthetic musks(SMs),and 5 UV filters(UVs)in water.No-tably,this method provided a decent linearity of calibration standards(R^(2)>0.999),excellent method limits of quantification(MLOQs)(0.12–11.41 ng/L),satisfactory matrix spiking recovery rates(60.4%–126%),and high precision(intra-day relative standard deviations(RSDs):1.0%–10.0%,inter-day RSDs:3.0%–15.0%,and inter-week RSDs:3.4%–15.7%),making it suitable for trace-level studies.Statistical analysis revealed that SVOCs with higher volatility exhibited enhanced recovery rates.Validation of the methodology involved analyzing SVOCs in real spring water and river water samples.Twenty-seven SVOCs were detected in spring water and 58 in river water,with an average concentration of 631.73 and 16,095 ng/L,respectively.Among the detected SVOCs,PAEs constituted the predominant proportion.This study underscored the presence of SVOCs contamination specifi-cally within the spring water,although SVOCs concentrations in river water were significantly greater than those found in spring water.In summary,this sensitive method based on SPE–GC–MS/MS was successfully developed and validated for the rapid analysis of a diverse array of 256 SVOCs at trace levels in water,including not only the traditional highly valued PAHs,PCBs,pesticides,and PAEs,but also the emerging OPEs,UVs,and SMs.展开更多
The application of therapeutic herbs in treatment of common infections and diseases has gained popularity in developing nations due to its cost-effectiveness and purported lower side effects compared to synthetic drug...The application of therapeutic herbs in treatment of common infections and diseases has gained popularity in developing nations due to its cost-effectiveness and purported lower side effects compared to synthetic drugs.Fagara zanthoxyloides Lam also known as Zanthoxylum zanthoxyloides,is an indigenous plant used widely as chewing stick for tooth cleaning in West Africa.The root-bark macerations,decoctions or infusions are the most valuable organs widely taken to treat malaria and diabetes by local populations,traditional healers and local pharmaceutical firms.Searching alternative,more efficient,safer,and less expensive anti-diabetic natural products from plants has received great attention,hence this research delved into the inhibitory effects of the ethyl acetate fraction of F.zanthoxyloides root-bark(EAFFZRB)on in-vitroα-amylase andα-glucosidase enzymes.Standard biochemical methods were used to extract and assess the in-vitro antioxidant such as 2,2-diphenyl-1-picrylhydrazl(DPPH),total antioxidant capacity(TAC)and Ferric Reducing Antioxidant Power(FRAP).The sample was combined at different concentrations(10,20,40,80,160,320,and 640μl/dl).Theα-amylase,andα-glucosidase activities of EAFFZRB sample was combined at different concentrations(0.2,0.4,0.6,0.8 and 1.0 mg/mL).The phyto-constituents in EAFFZRB were identified using Gas Chromatography-Mass Spectrometry(GC-MS)technique.The plant fraction showed a significant(P<0.05)increase in the inhibition ofα-amylase IC50=0.726 andα-glucosidase IC50=0.877 at varying concentrations,comparable to the reference drug Acarbose IC50=0.728 and IC50=0.852 respectively.EAFFZRB demonstrated significant(P<0.05)inhibition at different concentrations on DPPH(66.55±1.57),TAC(0.47±0.17)and FRAP(3.64±0.14),similar to the effect of the standard ascorbic acid.The GC-MS analysis of EAFFZRB revealed a total of 12 volatile organic compounds such as;Phenol-3-methoxy-2,5,6-trimethyl,2-Hydroxy-5-nitrobenzylbromide,2,6-Dimethyl-4-(3-nitrophenyl)pyridine,1H-Benz[e]indene,2-methyl and others.The study confirmed the in-vitro anti-diabetic and anti-oxidant properties of EAFFZRB,supporting the traditional use of the plant which might have acted additively or in synergy towards potentiating of antidiabetic effect observed,and it could be beneficial to the pharmaceutical industry if utilized.展开更多
Pyropia yezoensis(red algae)or commonly known as nori,is highly regarded for its nutritional benefits and distinct taste,leading to its widespread consumption.The bio-activity and sensory characteristics of P.yezoensi...Pyropia yezoensis(red algae)or commonly known as nori,is highly regarded for its nutritional benefits and distinct taste,leading to its widespread consumption.The bio-activity and sensory characteristics of P.yezoensis are attributed to the metabolites it contains.In this study,identification and quantification of the diverse range of metabolites of P.yezoensis and metabolomic analysis were conducted using gas chromatography-mass spectrometry(GC-MS).Furthermore,the impact of high temperature on its metabolites regulation was also investigated.Due to metabolomic analysis,a diverse range of metabolites were identified in P.yezoensis,including lipids,amino acids,carbohydrates,and secondary metabolites.Several known bioactive compounds,including alcohol and polyols,amines,amino acids-peptides-analogues,beta hydroxy acids and derivatives,carbohydrates and carbohydrate conjugates,cholestane steroids,dicarboxylic acid and derivatives,and fatty acids and conjugates were detected in abundance,highlighting the nutritional and functional properties of P.yezoensis.Additionally,the metabolites composition of P.yezoensis was significantly affected in high temperatures,which led to up-regulation of considerable primary metabolites and few were down-regulated,and suggested a potential response and adaptation mechanism of P.yezoensis to elevated temperature conditions.This research highlighted the metabolomics of P.yezoensis,provided insights into its metabolite composition and regulatory responses to high temperature conditions,enhanced our knowledge of the biochemical pathways and adaptive mechanisms of P.yezoensis,which can assist the improvement strategies of utilization and cultivation to promote this valuable alga in response to fluctuating environmental conditions.展开更多
本文通过以玉米碴为原料,酿酒酵母、嗜酸乳杆菌、枯草芽孢杆菌及纳豆芽孢杆菌作发酵剂,以单菌和组合的方式对玉米进行发酵制成玉米粉,采用气相-离子迁移谱(Gas-chromatography ion mobility spectrometry,GCIMS)和气相色谱-质谱联用(Gas...本文通过以玉米碴为原料,酿酒酵母、嗜酸乳杆菌、枯草芽孢杆菌及纳豆芽孢杆菌作发酵剂,以单菌和组合的方式对玉米进行发酵制成玉米粉,采用气相-离子迁移谱(Gas-chromatography ion mobility spectrometry,GCIMS)和气相色谱-质谱联用(Gas Chromatography-Mass Spectrometry,GC-MS)方法对发酵玉米粉的挥发性风味成分进行分析。结果表明,GC-IMS共检出68种风味物质,包括醛类12种、醇类18种、酯类11种、酮类11种以及6种杂环类;根据图谱差异分析发现,发酵后玉米粉风味物质均发生变化,其醇类、酯类和酸类物质含量相对提高。GC-MS共检出59种风味物质,包含13种醛类、12种酯类、15种醇类、7种酸类、6种酮类和7种杂环类物质。两种技术共同检测出29种风味物质,其中正癸醛等10种醛类、5种醇类、2-壬酮、2-庚酮、己酸乙酯、甲酸甲酯、丁酸乙酯及4种酸类物质为发酵玉米粉的主要风味物质。根据香气含量分析,对比未发酵玉米粉,自然发酵水果香含量提高了24.64%,酿酒酵母发酵水果香和酒香含量分别提高了30.05%、85.05%,嗜酸乳杆菌发酵水果甜香和奶油蜂蜜香含量分别提高了36.56%、90.36%,枯草芽孢杆菌和纳豆芽孢杆菌发酵水果甜香花及苦杏仁香含量分别提高了18.05%、15.59%和38.68%、38.02%,组合发酵水果甜香花、草香含量分别提高了53.53%、48.08%,表明经过发酵制得的玉米粉风味独特。展开更多
基金supported by the National Natural Science Foundation of China(No.82204610)Qihang Talent Program(L2022046)+1 种基金the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CI2021A04013)Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ15-YQ-041 and L2021029).
文摘Background:Cyperi Rhizoma,derived from Cyperus rotundus L.,is a widely used medicinal herb in traditional Chinese medicine(TCM),with Shandong Province recognized as its geo-authentic habitat.However,the quality of Cyperi Rhizoma varies significantly across different regions,potentially influencing its therapeutic efficacy.This study investigates the influence of geographic origin on the chemical composition and overall quality of Cyperi Rhizoma.Methods:A comprehensive approach,including traditional quality assessment,GC-MS(g as c hromatography-m ass s pectrometry),RP-HPLC(r everse p hase h igh-p erformance l iquid c hromatography),and FT-IR(f ourier t ransform i nfrared s pectroscopy)techniques,was employed to analyze Cyperi Rhizoma samples from Shandong Province.These methods examined the physical appearance,chemical profile,and content variations,particularly focusing onα-cyperone.Results:Traditional quality assessments revealed noticeable differences in the external characteristics of the samples.GC-MS analysis identified a variety of unique chemical constituents,while RP-HPLC and FT-IR showed significant variations inα-cyperone content,with higher levels found in Shandong samples.Conclusion:These results demonstrate that geographic origin is a critical determinant of Cyperi Rhizoma quality,with Shandong specimens exhibiting superiorα-cyperone levels and characteristic phytochemical profiles.This validates the geo-authenticity concept in TCM and provides actionable data for developing evidence-based quality standards,suggesting that provenance should be prioritized in medicinal material selection and pharmacopeial specifications.
基金supported by the National Natural Science Foundation of China(No.51939009)Shenzhen Science and Technology Program(Nos.JCYJ20241202125905008 and GXWD20201231165807007-20200810165349001).
文摘A trace analytical method based on solid-phase extraction gas chromatography-tandem mass spectrometry(SPE–GC–MS/MS)was developed for the rapid detection of 256 semi-volatile organic compounds(SVOCs),including 25 polycyclic aromatic hydrocarbons(PAHs),70 polychlorinated biphenyls(PCBs),123 pesticides,20 phthalate esters(PAEs),4 organophosphate esters(OPEs),9 synthetic musks(SMs),and 5 UV filters(UVs)in water.No-tably,this method provided a decent linearity of calibration standards(R^(2)>0.999),excellent method limits of quantification(MLOQs)(0.12–11.41 ng/L),satisfactory matrix spiking recovery rates(60.4%–126%),and high precision(intra-day relative standard deviations(RSDs):1.0%–10.0%,inter-day RSDs:3.0%–15.0%,and inter-week RSDs:3.4%–15.7%),making it suitable for trace-level studies.Statistical analysis revealed that SVOCs with higher volatility exhibited enhanced recovery rates.Validation of the methodology involved analyzing SVOCs in real spring water and river water samples.Twenty-seven SVOCs were detected in spring water and 58 in river water,with an average concentration of 631.73 and 16,095 ng/L,respectively.Among the detected SVOCs,PAEs constituted the predominant proportion.This study underscored the presence of SVOCs contamination specifi-cally within the spring water,although SVOCs concentrations in river water were significantly greater than those found in spring water.In summary,this sensitive method based on SPE–GC–MS/MS was successfully developed and validated for the rapid analysis of a diverse array of 256 SVOCs at trace levels in water,including not only the traditional highly valued PAHs,PCBs,pesticides,and PAEs,but also the emerging OPEs,UVs,and SMs.
文摘The application of therapeutic herbs in treatment of common infections and diseases has gained popularity in developing nations due to its cost-effectiveness and purported lower side effects compared to synthetic drugs.Fagara zanthoxyloides Lam also known as Zanthoxylum zanthoxyloides,is an indigenous plant used widely as chewing stick for tooth cleaning in West Africa.The root-bark macerations,decoctions or infusions are the most valuable organs widely taken to treat malaria and diabetes by local populations,traditional healers and local pharmaceutical firms.Searching alternative,more efficient,safer,and less expensive anti-diabetic natural products from plants has received great attention,hence this research delved into the inhibitory effects of the ethyl acetate fraction of F.zanthoxyloides root-bark(EAFFZRB)on in-vitroα-amylase andα-glucosidase enzymes.Standard biochemical methods were used to extract and assess the in-vitro antioxidant such as 2,2-diphenyl-1-picrylhydrazl(DPPH),total antioxidant capacity(TAC)and Ferric Reducing Antioxidant Power(FRAP).The sample was combined at different concentrations(10,20,40,80,160,320,and 640μl/dl).Theα-amylase,andα-glucosidase activities of EAFFZRB sample was combined at different concentrations(0.2,0.4,0.6,0.8 and 1.0 mg/mL).The phyto-constituents in EAFFZRB were identified using Gas Chromatography-Mass Spectrometry(GC-MS)technique.The plant fraction showed a significant(P<0.05)increase in the inhibition ofα-amylase IC50=0.726 andα-glucosidase IC50=0.877 at varying concentrations,comparable to the reference drug Acarbose IC50=0.728 and IC50=0.852 respectively.EAFFZRB demonstrated significant(P<0.05)inhibition at different concentrations on DPPH(66.55±1.57),TAC(0.47±0.17)and FRAP(3.64±0.14),similar to the effect of the standard ascorbic acid.The GC-MS analysis of EAFFZRB revealed a total of 12 volatile organic compounds such as;Phenol-3-methoxy-2,5,6-trimethyl,2-Hydroxy-5-nitrobenzylbromide,2,6-Dimethyl-4-(3-nitrophenyl)pyridine,1H-Benz[e]indene,2-methyl and others.The study confirmed the in-vitro anti-diabetic and anti-oxidant properties of EAFFZRB,supporting the traditional use of the plant which might have acted additively or in synergy towards potentiating of antidiabetic effect observed,and it could be beneficial to the pharmaceutical industry if utilized.
基金Supported by the National Key R&D Program of China(No.2016 YFC 1402102)the National Natural Science Foundation of China(No.41976109)+1 种基金the Ministry of Natural Resources Key Laboratory of Eco-Environmental Science and Technology of China(No.MEEST-2020-2)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Pyropia yezoensis(red algae)or commonly known as nori,is highly regarded for its nutritional benefits and distinct taste,leading to its widespread consumption.The bio-activity and sensory characteristics of P.yezoensis are attributed to the metabolites it contains.In this study,identification and quantification of the diverse range of metabolites of P.yezoensis and metabolomic analysis were conducted using gas chromatography-mass spectrometry(GC-MS).Furthermore,the impact of high temperature on its metabolites regulation was also investigated.Due to metabolomic analysis,a diverse range of metabolites were identified in P.yezoensis,including lipids,amino acids,carbohydrates,and secondary metabolites.Several known bioactive compounds,including alcohol and polyols,amines,amino acids-peptides-analogues,beta hydroxy acids and derivatives,carbohydrates and carbohydrate conjugates,cholestane steroids,dicarboxylic acid and derivatives,and fatty acids and conjugates were detected in abundance,highlighting the nutritional and functional properties of P.yezoensis.Additionally,the metabolites composition of P.yezoensis was significantly affected in high temperatures,which led to up-regulation of considerable primary metabolites and few were down-regulated,and suggested a potential response and adaptation mechanism of P.yezoensis to elevated temperature conditions.This research highlighted the metabolomics of P.yezoensis,provided insights into its metabolite composition and regulatory responses to high temperature conditions,enhanced our knowledge of the biochemical pathways and adaptive mechanisms of P.yezoensis,which can assist the improvement strategies of utilization and cultivation to promote this valuable alga in response to fluctuating environmental conditions.
