The Barley yellow dwarf virus (BYDV) GAV isolate was preserved at the Institute of Plant Protection of the Chinese Academy of Agricultural Sciences. The cDNA of BYDV GAV coat protein (CP) gene was amplified from the e...The Barley yellow dwarf virus (BYDV) GAV isolate was preserved at the Institute of Plant Protection of the Chinese Academy of Agricultural Sciences. The cDNA of BYDV GAV coat protein (CP) gene was amplified from the extracted RNA of BYDV GAV by using the polymerase chain reaction (PCR), and cloned into pGEM-7zf(+). Its complete nucleotide sequence has been determined by means of Sanger’s dideoxy-mediated chain-termination method. The result showed that BYDV GAV CP gene has 600nt. It shares 97.5% and 96.5% identity with CP gene of BYDV MAV-PS1 in terms of nucleotide and amino acid sequences respectively.展开更多
The IPTG inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E.coli BL21(DE3).High level expression of the specific protein was achieved by IPTG induction.The res...The IPTG inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E.coli BL21(DE3).High level expression of the specific protein was achieved by IPTG induction.The results of SDS PAGE and Western blottong show that the expression product which accumulates 19.5% of the total cellular proteins estimated by scanning is 24 kD BYDV GAV coat protein plus eleven amino acids of pET 5a.展开更多
The complete nucleotide sequence of genomic RNA of BYDV-GAV was determined. It comprised 5685 nucleotides and contained six open reading frames and four un-translated regions. The size and organization of BYDV-GAV gen...The complete nucleotide sequence of genomic RNA of BYDV-GAV was determined. It comprised 5685 nucleotides and contained six open reading frames and four un-translated regions. The size and organization of BYDV-GAV genome were similar to those of BYDV PAV-aus. The nucleotide and deduced amino acid sequences of the six ORFs were aligned and compared with those of other luteoviruses. The results showed that there was a high degree of identity between BYDV-GAV and MAV-PS1 in all ORFs except ORF5 and ORF6, which had only 87.4% and 70.2% identities respectively. The reported genomic nucleotide sequence of MAV was shorter than that of BYDV-GAV, but the comparison of the genomic nucleotide sequences for MAV-PS1 and GAV showed 90.4% sequence identity for the same region of the genome. Ac-cording to the level of sequence similarities, BYDV-GAV should be closely related to BYDV-MAV.展开更多
法藏敦煌藏文文书P.t.1097《官府支出粮食清册》应该是吐蕃统治敦煌时期当地官府支出粮食等物品的账目记录清单,文书中记载的职官押衙(am va gav)、长史(jang shi,亦可译为长使)、人名ya ya在吐蕃统治敦煌时期文书中已出现,并非只是在...法藏敦煌藏文文书P.t.1097《官府支出粮食清册》应该是吐蕃统治敦煌时期当地官府支出粮食等物品的账目记录清单,文书中记载的职官押衙(am va gav)、长史(jang shi,亦可译为长使)、人名ya ya在吐蕃统治敦煌时期文书中已出现,并非只是在归义军时期才出现的职官,不能据此将该文书定为归义军时期文书。展开更多
GAV 车辆终端系统(具有监控、定位、报警综合功能的设备)是落实科技部下达给深圳"十五"科技攻关智能交通工程示范市(编号:2002BA404A12),研究实现对车辆跟踪、调度的创新技术(专利号02124101.5)。2003年被评为深圳市科技进步...GAV 车辆终端系统(具有监控、定位、报警综合功能的设备)是落实科技部下达给深圳"十五"科技攻关智能交通工程示范市(编号:2002BA404A12),研究实现对车辆跟踪、调度的创新技术(专利号02124101.5)。2003年被评为深圳市科技进步二等奖,现运用于广东和江苏等地的公交巴士和珠江口船舶 GPS 定位和图像监控。展开更多
文摘The Barley yellow dwarf virus (BYDV) GAV isolate was preserved at the Institute of Plant Protection of the Chinese Academy of Agricultural Sciences. The cDNA of BYDV GAV coat protein (CP) gene was amplified from the extracted RNA of BYDV GAV by using the polymerase chain reaction (PCR), and cloned into pGEM-7zf(+). Its complete nucleotide sequence has been determined by means of Sanger’s dideoxy-mediated chain-termination method. The result showed that BYDV GAV CP gene has 600nt. It shares 97.5% and 96.5% identity with CP gene of BYDV MAV-PS1 in terms of nucleotide and amino acid sequences respectively.
文摘The IPTG inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E.coli BL21(DE3).High level expression of the specific protein was achieved by IPTG induction.The results of SDS PAGE and Western blottong show that the expression product which accumulates 19.5% of the total cellular proteins estimated by scanning is 24 kD BYDV GAV coat protein plus eleven amino acids of pET 5a.
基金This work was supported by the National Key Basic Research of China(973 contract TG2000016201)the National Natural Science Foundation of China(Grant No.39970034).
文摘The complete nucleotide sequence of genomic RNA of BYDV-GAV was determined. It comprised 5685 nucleotides and contained six open reading frames and four un-translated regions. The size and organization of BYDV-GAV genome were similar to those of BYDV PAV-aus. The nucleotide and deduced amino acid sequences of the six ORFs were aligned and compared with those of other luteoviruses. The results showed that there was a high degree of identity between BYDV-GAV and MAV-PS1 in all ORFs except ORF5 and ORF6, which had only 87.4% and 70.2% identities respectively. The reported genomic nucleotide sequence of MAV was shorter than that of BYDV-GAV, but the comparison of the genomic nucleotide sequences for MAV-PS1 and GAV showed 90.4% sequence identity for the same region of the genome. Ac-cording to the level of sequence similarities, BYDV-GAV should be closely related to BYDV-MAV.
文摘法藏敦煌藏文文书P.t.1097《官府支出粮食清册》应该是吐蕃统治敦煌时期当地官府支出粮食等物品的账目记录清单,文书中记载的职官押衙(am va gav)、长史(jang shi,亦可译为长使)、人名ya ya在吐蕃统治敦煌时期文书中已出现,并非只是在归义军时期才出现的职官,不能据此将该文书定为归义军时期文书。
