To isolate the cDNA partial sequence of key enzyme gene GalAT for pectin biosynthesis in ramie [Boehmeria nivea （L.） Gaud], and thus to understand the expression of GalAT gene in different tissues of ramie, degenera...To isolate the cDNA partial sequence of key enzyme gene GalAT for pectin biosynthesis in ramie [Boehmeria nivea （L.） Gaud], and thus to understand the expression of GalAT gene in different tissues of ramie, degenerate primer was designed according to GalAT conserved sequence in other species reported, and the cDNA sequence of GalAT gene from ramie variety Zhongzhu 1 was cloned by RT-PCR method based on the degenerate primer. The cDNA revealed a 986-bp in length which encoded 328 amino acids. The cDNA sequence and putative amino acid sequence of GalAT shared high identity with previously reported Arabidopsis thaliana GA UT4 （GalAT） as 77 and 83%, respectively. Molecular evolution analysis showed that the putative amino acid sequence and Arabidopsis thaliana GAUT4 gathered to a same group. Real-time quantitative PCR analysis showed that GalAT mRNA accumulated most abundantly in root, and GalAT transcripts in all kinds of ramie tissues in turn revealed as follows： root 〉 leaf〉 bast 〉 or ≈ xylem.展开更多
正The Understanding and Cooperation Dialogue, Sponsored by the Chinese Association for International Understanding (CAFIU) and the China Foundation for Peace and Development (CFPD), and Co-organized by the Chinese Peo...正The Understanding and Cooperation Dialogue, Sponsored by the Chinese Association for International Understanding (CAFIU) and the China Foundation for Peace and Development (CFPD), and Co-organized by the Chinese People' s Association for Peace and展开更多
MEET in Beijing 2002, an international gala month, tookplace last May in China’s capital. The theme of this month-long festival was to "enjoy arts, beauty, hope, and the spring."
基金supported by the National 863 Program of China (2001AA241211)
文摘To isolate the cDNA partial sequence of key enzyme gene GalAT for pectin biosynthesis in ramie [Boehmeria nivea （L.） Gaud], and thus to understand the expression of GalAT gene in different tissues of ramie, degenerate primer was designed according to GalAT conserved sequence in other species reported, and the cDNA sequence of GalAT gene from ramie variety Zhongzhu 1 was cloned by RT-PCR method based on the degenerate primer. The cDNA revealed a 986-bp in length which encoded 328 amino acids. The cDNA sequence and putative amino acid sequence of GalAT shared high identity with previously reported Arabidopsis thaliana GA UT4 （GalAT） as 77 and 83%, respectively. Molecular evolution analysis showed that the putative amino acid sequence and Arabidopsis thaliana GAUT4 gathered to a same group. Real-time quantitative PCR analysis showed that GalAT mRNA accumulated most abundantly in root, and GalAT transcripts in all kinds of ramie tissues in turn revealed as follows： root 〉 leaf〉 bast 〉 or ≈ xylem.
文摘正The Understanding and Cooperation Dialogue, Sponsored by the Chinese Association for International Understanding (CAFIU) and the China Foundation for Peace and Development (CFPD), and Co-organized by the Chinese People' s Association for Peace and
文摘MEET in Beijing 2002, an international gala month, tookplace last May in China’s capital. The theme of this month-long festival was to "enjoy arts, beauty, hope, and the spring."
文摘四星级的Vincci Gala酒店是由位于巴塞罗那扩展区（Eixample）的一栋历史建筑改建而来,酒店拥有78间客房以及酒吧、餐厅、会议室和各式露台。酒店的设计因两个要求而独具特点：一是巴塞罗那古迹保护部（the Monument Protection Department of Barcelona）对于建筑外立面祁建筑内部白色大理石楼梯的保护要求；二是酒店要以加拉（Gala,超现实主义画家萨尔瓦多,达利的妻子,他的缪斯的名字）命名的要求。
