[Objective]The study aimed to screen the starch-degrading bacterium in bagasse and carry on the identification of strains s2g5-1 and s3g4-8.[Method]By using a variety of selective media,varieties of starch degrading b...[Objective]The study aimed to screen the starch-degrading bacterium in bagasse and carry on the identification of strains s2g5-1 and s3g4-8.[Method]By using a variety of selective media,varieties of starch degrading bacterium were isolated from the sugar cane bagasse form different stages of natural fermentation,then,primary screening and secondary screening were performed.[Result] Starch-degrading strains s2g5-1 and s3g4-8 were screened,and they were identified as Bacillus amyloliquefaciens according to their morphological,physiological,biochemical and molecular characteristics.[Conclusion]The research provided theoretical basis for factory application of bagasse.展开更多
To obtain single chain variable fragment （scFv） and bivalent single chain variable fragment （bsFv） against transferrin receptor, up-stream and down-stream primers were designed according to the complementary seque...To obtain single chain variable fragment （scFv） and bivalent single chain variable fragment （bsFv） against transferrin receptor, up-stream and down-stream primers were designed according to the complementary sequences of FR1 region of variable heavy （VH） and FR4 of variable light （VL）, respectively, which contained inter-linker G4S and the restriction endonuclease SfiI, AscI and NotI. Two pieces of scFv fragments were first amplified through PCR and then inserted into plasmid pAB1, which could express scFv protein once induced by IPTG in the host bacteria. To express scFv and bsFv, E. coli TG1 was cultured in LB broth and was induced by IPTG. The restriction enzyme digestion map and DNA sequencing demonstrated that scFv and bsFv genes were successfully inserted into the expression plasmid. SDS-PAGE and Western blotting revealed the protein band at 35kD and 60kD, which were consistent with the molecular weight of scFv and bsFv respectively. Flow cytometry showed that scFv and bsFv harbored the specific binding activity with TfR expressed in various tumor cells, and the avidity of bsFv was higher than that of the parent scFv.展开更多
AIM: To investigate the association between autoimmune pancreatitis (AIP) and systemic autoimmune diseases (SAIDs) by measurement of serum immunoglobulin G4 (IgG4). METHODS: The serum level of IgG4 was measured in 61 ...AIM: To investigate the association between autoimmune pancreatitis (AIP) and systemic autoimmune diseases (SAIDs) by measurement of serum immunoglobulin G4 (IgG4). METHODS: The serum level of IgG4 was measured in 61 patients with SAIDs of different types who had not yet participated in glucocorticosteroid treatment. Patients with an elevated IgG4 level were examined by abdominal ultrasonography (US) and, in some cases, by computer tomography (CT). RESULTS: Elevated serum IgG4 levels (919 ± 996 mg/L) were detected in 17 (28%) of the 61 SAID patients. 10 patients had Sj gren's syndrome (SS) (IgG4: 590 ± 232 mg/L), 2 of them in association with Hashimoto's thyroiditis, and 7 patients (IgG4: 1388 ± 985.5 mg/L) had systemic lupus erythematosus (SLE). The IgG4 level in the SLE patients and that in patients with SS were not significantly different from that in AIP patients (783 ± 522 mg/L). Abdominal US and CT did not reveal any characteristic features of AIP among the SAID patients with an elevated IgG4 level. CONCLUSION: The serum IgG4 level may be elevated in SAIDs without the presence of AIP. The determination of serum IgG4 does not seem to be suitable for the differentiation between IgG4-related diseases and SAIDs.展开更多
Compared to the single-stranded and double-stranded types of classical nucleic acid structures,atypical nucleic acid structures(such as G4s,i-motif,Triplex,and cyclic nucleic acids)are gradually becoming hotspots in b...Compared to the single-stranded and double-stranded types of classical nucleic acid structures,atypical nucleic acid structures(such as G4s,i-motif,Triplex,and cyclic nucleic acids)are gradually becoming hotspots in biomedical research due to their important biological functions and the close correlation between their abnormal dynamics equilibrium in physiological environments and a variety of hard-tackle diseases.The traditional gel electrophoresis,nuclear magnetic resonance,and circular dichroism detection techniques have shortcomings such as low spatial resolution,high destructiveness,and lack of real-time dynamic monitoring capability.In recent years,fluorescence imaging has gradually become a cutting-edge tool for non-classical nucleic acid structure detection due to their high sensitivity,fast response and dynamic real-time observation performance.In this contribution,we review the fluorescence materials for lighting-up imaging of non-classical nucleic acid structures,including traditional fluorescent small molecules and aggregation-induced emission luminogens(AIEgens).The design principles,detection mechanisms and application scenarios are detailed.Current fluorescence probes have already improved qualities in recognition targetability and signal-to-noise ratio by tuning and optimizing molecular structure-property relationships,but still face challenges such as insufficient selectivity and poor penetration capability in vivo.In the future,it is necessary to integrate multimodal imaging,artificial intelligence-assisted design and targeted delivery system to build a highly sensitive and multi-channel responsive platform to thoroughly disclose the association between the dynamic conformation of nucleic acid and disease,and to promote the development of precise and novel therapeutic strategies.展开更多
基金Supported by the Central Public-interest Scientific Institution Basal Research Fund(2008hzs1J021,2009hzs1J033)~~
文摘[Objective]The study aimed to screen the starch-degrading bacterium in bagasse and carry on the identification of strains s2g5-1 and s3g4-8.[Method]By using a variety of selective media,varieties of starch degrading bacterium were isolated from the sugar cane bagasse form different stages of natural fermentation,then,primary screening and secondary screening were performed.[Result] Starch-degrading strains s2g5-1 and s3g4-8 were screened,and they were identified as Bacillus amyloliquefaciens according to their morphological,physiological,biochemical and molecular characteristics.[Conclusion]The research provided theoretical basis for factory application of bagasse.
基金supported by a grant from "863" program of China (No. 2006AA02Z158)the Ministry of Education Science Foundation of China (No. 20060487024)Science and Technology project of Jiangxi Province Education Department (No. 2006-86).
文摘To obtain single chain variable fragment （scFv） and bivalent single chain variable fragment （bsFv） against transferrin receptor, up-stream and down-stream primers were designed according to the complementary sequences of FR1 region of variable heavy （VH） and FR4 of variable light （VL）, respectively, which contained inter-linker G4S and the restriction endonuclease SfiI, AscI and NotI. Two pieces of scFv fragments were first amplified through PCR and then inserted into plasmid pAB1, which could express scFv protein once induced by IPTG in the host bacteria. To express scFv and bsFv, E. coli TG1 was cultured in LB broth and was induced by IPTG. The restriction enzyme digestion map and DNA sequencing demonstrated that scFv and bsFv genes were successfully inserted into the expression plasmid. SDS-PAGE and Western blotting revealed the protein band at 35kD and 60kD, which were consistent with the molecular weight of scFv and bsFv respectively. Flow cytometry showed that scFv and bsFv harbored the specific binding activity with TfR expressed in various tumor cells, and the avidity of bsFv was higher than that of the parent scFv.
基金Supported by Grants TáMOP-4.2.1./B-09/1/KONV and 4.2.2-08/1-2008-0002 (partly)
文摘AIM: To investigate the association between autoimmune pancreatitis (AIP) and systemic autoimmune diseases (SAIDs) by measurement of serum immunoglobulin G4 (IgG4). METHODS: The serum level of IgG4 was measured in 61 patients with SAIDs of different types who had not yet participated in glucocorticosteroid treatment. Patients with an elevated IgG4 level were examined by abdominal ultrasonography (US) and, in some cases, by computer tomography (CT). RESULTS: Elevated serum IgG4 levels (919 ± 996 mg/L) were detected in 17 (28%) of the 61 SAID patients. 10 patients had Sj gren's syndrome (SS) (IgG4: 590 ± 232 mg/L), 2 of them in association with Hashimoto's thyroiditis, and 7 patients (IgG4: 1388 ± 985.5 mg/L) had systemic lupus erythematosus (SLE). The IgG4 level in the SLE patients and that in patients with SS were not significantly different from that in AIP patients (783 ± 522 mg/L). Abdominal US and CT did not reveal any characteristic features of AIP among the SAID patients with an elevated IgG4 level. CONCLUSION: The serum IgG4 level may be elevated in SAIDs without the presence of AIP. The determination of serum IgG4 does not seem to be suitable for the differentiation between IgG4-related diseases and SAIDs.
基金Project supported by the National Natural Science Foundation of China(No.22274106)the Program of Suzhou Innovation and Entrepreneurship Leading Talents(No.ZXL2022513)the Startup Funds from Soochow University。
文摘Compared to the single-stranded and double-stranded types of classical nucleic acid structures,atypical nucleic acid structures(such as G4s,i-motif,Triplex,and cyclic nucleic acids)are gradually becoming hotspots in biomedical research due to their important biological functions and the close correlation between their abnormal dynamics equilibrium in physiological environments and a variety of hard-tackle diseases.The traditional gel electrophoresis,nuclear magnetic resonance,and circular dichroism detection techniques have shortcomings such as low spatial resolution,high destructiveness,and lack of real-time dynamic monitoring capability.In recent years,fluorescence imaging has gradually become a cutting-edge tool for non-classical nucleic acid structure detection due to their high sensitivity,fast response and dynamic real-time observation performance.In this contribution,we review the fluorescence materials for lighting-up imaging of non-classical nucleic acid structures,including traditional fluorescent small molecules and aggregation-induced emission luminogens(AIEgens).The design principles,detection mechanisms and application scenarios are detailed.Current fluorescence probes have already improved qualities in recognition targetability and signal-to-noise ratio by tuning and optimizing molecular structure-property relationships,but still face challenges such as insufficient selectivity and poor penetration capability in vivo.In the future,it is necessary to integrate multimodal imaging,artificial intelligence-assisted design and targeted delivery system to build a highly sensitive and multi-channel responsive platform to thoroughly disclose the association between the dynamic conformation of nucleic acid and disease,and to promote the development of precise and novel therapeutic strategies.
