英雄联盟世界赛在欧洲举行,瑞士轮阶段,很多观众都听到了现场的“Let’s go G2”。现场观众支持的欧洲队伍被称为主场,G2和FNC在LEC的转播中心这片熟悉的场地比赛被称为主场,LNG九号电动的苏州阳澄国际电竞中心也被称为主场。这些主场...英雄联盟世界赛在欧洲举行,瑞士轮阶段,很多观众都听到了现场的“Let’s go G2”。现场观众支持的欧洲队伍被称为主场,G2和FNC在LEC的转播中心这片熟悉的场地比赛被称为主场,LNG九号电动的苏州阳澄国际电竞中心也被称为主场。这些主场好像不是一回事。展开更多
Gastrointestinal(GI)parasites are a global concern and cause serious damage to the health of animals,including horses,because of their deleterious effects on the health of these animals.Owing to improperly diagnosing ...Gastrointestinal(GI)parasites are a global concern and cause serious damage to the health of animals,including horses,because of their deleterious effects on the health of these animals.Owing to improperly diagnosing the parasitic load and the resulting incorrect treatment,anthelmintics are becoming a global problem.Although rapid diagnostic techniques such as McMaster and FECPAK^(G2) are available in Africa,concentration techniques,including flotation and sedimentation,are commonly used in screening for gastrointestinal parasites.In this study,we compared the sensitivity of diagnostic techniques,namely,McMaster and FECPAK^(G2),in quantifying and determining the prevalence of gastrointestinal parasites as an alternative to the commonly used flotation and sedimentation techniques for equine fecal samples in Jos,Plateau State,Nigeria.Three hundred fecal samples were collected from different locations.The instructions provided by the manufacturers of FECPAK^(G2) and McMasters were used as the methodology for egg count per gram of fecal matter,while parasite egg/ova identification was performed through sedimentation and flotation techniques.In general,a prevalence of 66.3%was recorded,with a higher prevalence on Babale ranch(84%)than on Jos Polo Club(62%)and Jos mounted troop(53%).A total of nine gastrointestinal parasites were identified via both sedimentation and flotation techniques:Strongylus spp.(19.3%)was the most prevalent parasite identified via the flotation method,whereas Gastrodiscus aegyptiacus(25.3%)was the most prevalent parasite identified via the sedimentation technique.In general,FECPAK^(G2) had a sensitivity of 86%,whereas McMaster had a sensitivity of 64%in terms of egg counts per gram of fecal sample.This finding suggests that equines in Jos are highly infected with a variety of gastrointestinal parasites to varying degrees,with Strongylus spp.and Gastrodiscus aegyptiacus being more prevalent.Although the parasite load was found to range from low to moderate levels,the majority of the equine samples contained<200 eggs per gram,suggesting a low infection rate via both the McMaster technique and the FECPAK^(G2) technique.On the basis of these findings,FECPAK^(G2) is recommended for rapid diagnosis because of its prompt outcome and infestation rate as well as ease of routine examination.Additionally,routine examination should be carried out to determine infestation by gastrointestinal parasites in all equine holdings and the effectiveness of the therapeutic agents used.展开更多
目的探讨X射线修复交叉互补蛋白1(X-ray repair cross-complementing protein 1,XRCC1)、三磷酸腺苷结合转运蛋白G超家族成员2(ATP-binding cassette superfamily G member 2,ABCG2)基因多态性与中晚期结直肠癌(colorectal cancer,CRC)...目的探讨X射线修复交叉互补蛋白1(X-ray repair cross-complementing protein 1,XRCC1)、三磷酸腺苷结合转运蛋白G超家族成员2(ATP-binding cassette superfamily G member 2,ABCG2)基因多态性与中晚期结直肠癌(colorectal cancer,CRC)患者铂类化疗敏感性的相关性。方法选择2021年1月至2022年8月温州医科大学附属平阳医院收治的75例中晚期CRC患者,均采用铂类药物化疗方案治疗24周。所有患者均于化疗前检测外周血XRCC1-Rs25487和ABCG2-Rs717620基因位点的多态性,探究其与患者2年总生存率的关系,采用比例风险(Cox)回归模型分析影响患者预后不良的因素。结果75例中晚期CRC患者的化疗总有效率为52.00%(39/75)。XRCC1-Rs25487基因型中,AA基因型携带者对铂类药物的化疗敏感性较GG+GA基因型携带者的化疗敏感性更低(P<0.05);ABCG2-Rs717620基因型中,TT基因型携带者对铂类药物的化疗敏感性较CC+CT基因型携带者的化疗敏感性更低(P<0.05)。Kaplan-Meier分析显示,XRCC1-Rs25487位点基因GA/GG型中位生存时间长于AA型;ABCG2-Rs717620位点基因CC/CT型中位生存时间长于TT型(P<0.05)。多因素Cox分析显示,铂类药物化疗效果和XRCC1-Rs25487位点基因AA型、ABCG2-Rs717620位点基因TT型均为中晚期CRC患者铂类药物化疗后2年总生存率的独立影响因素(P<0.05)。结论XRCC1、ABCG2基因多态性与中晚期CRC患者铂类药物化疗敏感性有关,XRCC1-Rs25487GG/GA基因型和ABCG2-Rs717620 CC/CT基因型可为中晚期CRC患者铂类化疗带来更好的药物敏感性和生存期。展开更多
文摘Gastrointestinal(GI)parasites are a global concern and cause serious damage to the health of animals,including horses,because of their deleterious effects on the health of these animals.Owing to improperly diagnosing the parasitic load and the resulting incorrect treatment,anthelmintics are becoming a global problem.Although rapid diagnostic techniques such as McMaster and FECPAK^(G2) are available in Africa,concentration techniques,including flotation and sedimentation,are commonly used in screening for gastrointestinal parasites.In this study,we compared the sensitivity of diagnostic techniques,namely,McMaster and FECPAK^(G2),in quantifying and determining the prevalence of gastrointestinal parasites as an alternative to the commonly used flotation and sedimentation techniques for equine fecal samples in Jos,Plateau State,Nigeria.Three hundred fecal samples were collected from different locations.The instructions provided by the manufacturers of FECPAK^(G2) and McMasters were used as the methodology for egg count per gram of fecal matter,while parasite egg/ova identification was performed through sedimentation and flotation techniques.In general,a prevalence of 66.3%was recorded,with a higher prevalence on Babale ranch(84%)than on Jos Polo Club(62%)and Jos mounted troop(53%).A total of nine gastrointestinal parasites were identified via both sedimentation and flotation techniques:Strongylus spp.(19.3%)was the most prevalent parasite identified via the flotation method,whereas Gastrodiscus aegyptiacus(25.3%)was the most prevalent parasite identified via the sedimentation technique.In general,FECPAK^(G2) had a sensitivity of 86%,whereas McMaster had a sensitivity of 64%in terms of egg counts per gram of fecal sample.This finding suggests that equines in Jos are highly infected with a variety of gastrointestinal parasites to varying degrees,with Strongylus spp.and Gastrodiscus aegyptiacus being more prevalent.Although the parasite load was found to range from low to moderate levels,the majority of the equine samples contained<200 eggs per gram,suggesting a low infection rate via both the McMaster technique and the FECPAK^(G2) technique.On the basis of these findings,FECPAK^(G2) is recommended for rapid diagnosis because of its prompt outcome and infestation rate as well as ease of routine examination.Additionally,routine examination should be carried out to determine infestation by gastrointestinal parasites in all equine holdings and the effectiveness of the therapeutic agents used.
文摘目的本研究旨在建立一种实时荧光定量PCR方法,用于检测猕猴三磷酸腺苷结合盒转运蛋白G2(adenosine triphosphate-binding cassette transporter protein G2,ABCG2)mRNA的基因转录水平。方法使用NCBI上GenBank数据库猕猴(Macaca mulatta)的ABCG2核苷酸序列号NM_001032919.1及内参GAPDH核苷酸序列号NM_001195426.1,借助Primer premier 5.0软件设计PCR引物。提取猕猴新鲜肾组织的总RNA,并反转录合成cDNA。接着,利用PCR引物进行实时荧光定量PCR扩增,并根据反应体系中荧光的变化情况定量分析ABCG2的mRNA相对表达水平。结果PCR产物测序结果显示,扩增的ABCG2和GAPDH核苷酸序列与NCBI上猕猴的序列同源性分别为90.91%和91.14%。ABCG2和GAPDH的扩增效率均达到80%~120%,实时荧光定量PCR标准曲线的熔解曲线为单峰,R2接近1。结论本研究建立的检测猕猴ABCG2 mRNA实时荧光定量检测方法,为研究高尿酸血症的发病机制以及新药开发奠定基础。
文摘目的探讨X射线修复交叉互补蛋白1(X-ray repair cross-complementing protein 1,XRCC1)、三磷酸腺苷结合转运蛋白G超家族成员2(ATP-binding cassette superfamily G member 2,ABCG2)基因多态性与中晚期结直肠癌(colorectal cancer,CRC)患者铂类化疗敏感性的相关性。方法选择2021年1月至2022年8月温州医科大学附属平阳医院收治的75例中晚期CRC患者,均采用铂类药物化疗方案治疗24周。所有患者均于化疗前检测外周血XRCC1-Rs25487和ABCG2-Rs717620基因位点的多态性,探究其与患者2年总生存率的关系,采用比例风险(Cox)回归模型分析影响患者预后不良的因素。结果75例中晚期CRC患者的化疗总有效率为52.00%(39/75)。XRCC1-Rs25487基因型中,AA基因型携带者对铂类药物的化疗敏感性较GG+GA基因型携带者的化疗敏感性更低(P<0.05);ABCG2-Rs717620基因型中,TT基因型携带者对铂类药物的化疗敏感性较CC+CT基因型携带者的化疗敏感性更低(P<0.05)。Kaplan-Meier分析显示,XRCC1-Rs25487位点基因GA/GG型中位生存时间长于AA型;ABCG2-Rs717620位点基因CC/CT型中位生存时间长于TT型(P<0.05)。多因素Cox分析显示,铂类药物化疗效果和XRCC1-Rs25487位点基因AA型、ABCG2-Rs717620位点基因TT型均为中晚期CRC患者铂类药物化疗后2年总生存率的独立影响因素(P<0.05)。结论XRCC1、ABCG2基因多态性与中晚期CRC患者铂类药物化疗敏感性有关,XRCC1-Rs25487GG/GA基因型和ABCG2-Rs717620 CC/CT基因型可为中晚期CRC患者铂类化疗带来更好的药物敏感性和生存期。
