【目的】比较竹山一号、恩标、龙井43等9个茶树品种对假眼小绿叶蝉(Empoasca vitis G the)的抗性水平,为茶树品种抗虫性机理研究奠定基础。【方法】调查叶蝉在不同品种上的田间数量动态、生命周期、每雌产若量和若虫成活率,并用刺探电...【目的】比较竹山一号、恩标、龙井43等9个茶树品种对假眼小绿叶蝉(Empoasca vitis G the)的抗性水平,为茶树品种抗虫性机理研究奠定基础。【方法】调查叶蝉在不同品种上的田间数量动态、生命周期、每雌产若量和若虫成活率,并用刺探电位图谱(electrical penetration graph,EPG)技术记录叶蝉的取食行为。【结果】①叶蝉在恩标、竹山一号、斑竹园和蓝天上的田间虫口密度普遍大于德清、建德、长兴紫笋和举岩;②德清、举岩、长兴紫笋、建德和龙井43上叶蝉的生命周期显著长于恩标、斑竹园和竹山一号;③恩标和斑竹园上小绿叶蝉每雌产若量明显多于举岩、长兴紫笋、建德和龙井43;④恩标和竹山一号上若虫成活率最高,分别为55.00%和50.00%;举岩上若虫成活率最低,仅为32.00%;⑤竹山一号和恩标上叶蝉取食时间显著长于德清、举岩、长兴紫笋、建德和龙井43,与蓝天和斑竹园没有显著差异;⑥使用聚类分析评价9个茶树品种抗叶蝉能力,将其分为两大类。第Ⅰ类:举岩、德清、龙井43、建德和长兴紫笋,对假眼小绿叶蝉抗性较强;第Ⅱ类:蓝天、斑竹园、竹山一号和恩标,对假眼小绿叶蝉抗性较弱。不同品种对叶蝉的抗性从强至弱的顺序为:举岩>德清、龙井43、建德和长兴紫笋>蓝天、斑竹园和竹山一号>恩标。【结论】田间虫口密度、生命周期、每雌产若量、若虫成活率和取食时间能够体现假眼小绿叶蝉在茶树品种上的自然选择、适应度和取食偏好,在分类茶树品种抗叶蝉水平上存在明显差异。展开更多
An electrochemical quantitative method of serum albumin was developed based on the orange G(OG)as probe.In pH 2.0 Britton-Robinson(B-R)buffer solution,OG has a sensitive reductive peak at -0.27V(vs.SCE)on the hanging ...An electrochemical quantitative method of serum albumin was developed based on the orange G(OG)as probe.In pH 2.0 Britton-Robinson(B-R)buffer solution,OG has a sensitive reductive peak at -0.27V(vs.SCE)on the hanging mercury drop electrode.After adding human serum albumin(HSA)to the above solution,the reduction peak current of OG decreases apparently without the shift of the peak potential.The decrease of the reduction peak current of OG is in linear with HSA concentration from 4.0 to 28.0mg/L with the regression equation △ip″(nA)=85.02+15.93C(mg/L),γ=0.993.This method was further applied to the determination of the content of HSA in blood samples and the results were in accordance with the traditional Coomassie brilliant blue G-250 spectrophotometric assay.展开更多
To compare the histological effects of trans-scleral cyclophotocoagulation(TCP) performed with two different probes, the G-probe(IRIDEX Medical Instruments, Mountain View, CA, USA) and the Ciliprobe(Katalyst Surgical,...To compare the histological effects of trans-scleral cyclophotocoagulation(TCP) performed with two different probes, the G-probe(IRIDEX Medical Instruments, Mountain View, CA, USA) and the Ciliprobe(Katalyst Surgical, Chesterfield, MO, USA). TCP was performed on two human cadaver eyes from the same corpse. The vertical meridian was marked and opposite sides were treated using either the G-probe or Ciliprobe. The first eye was treated with each probe at 2000 ms/2000 mW and the second eye at 3000 ms/1500 mW. Histological examination revealed separation and loss of the non-pigmented ciliary epithelium as well as vacuolization in all sections for both probes and settings. Changes to the non-pigmented ciliary epithelium treated at 3000 ms/1500 mW were similar between the two probes. A slightly more complete separation of the non-pigmented epithelium was noted on the Ciliprobe treated sections as compared to the G-probe treated sections in the eye treated at 2000 ms/2000 mW. Therefore, in human cadaver eyes, both the G-probe and Ciliprobe produced separation, vacuolization, and loss of the non-pigmented ciliary epithelium at two different, clinically utilized settings.展开更多
基金This work was supported by Natural Science Key Research Project of Anhui Provincial EducationDepartment(KJ2007A131ZC)Professor/Doctor Foundation of Suzhou College(2006jb05)
文摘利用光谱探针技术研究在酸性溶液中考马斯亮蓝G-250(Coomassie brilliant blue G-250,CBBG)与牛血清白蛋白(bovine serum albumin,BSA)相互作用机理,考察了不同实验条件对CBBG-BSA复合物吸收光谱的影响。实验结果表明:CBBG与BSA相互作用产生光谱蓝移主要是由CBBG与BSA间的疏水相互作用引起,而静电作用则是形成CBBG-BSA蓝色复合物的必要条件。同时,CBBG聚集体的聚集程度是影响CBBG-BSA蓝色复合物形成的重要因素。
文摘An electrochemical quantitative method of serum albumin was developed based on the orange G(OG)as probe.In pH 2.0 Britton-Robinson(B-R)buffer solution,OG has a sensitive reductive peak at -0.27V(vs.SCE)on the hanging mercury drop electrode.After adding human serum albumin(HSA)to the above solution,the reduction peak current of OG decreases apparently without the shift of the peak potential.The decrease of the reduction peak current of OG is in linear with HSA concentration from 4.0 to 28.0mg/L with the regression equation △ip″(nA)=85.02+15.93C(mg/L),γ=0.993.This method was further applied to the determination of the content of HSA in blood samples and the results were in accordance with the traditional Coomassie brilliant blue G-250 spectrophotometric assay.
文摘To compare the histological effects of trans-scleral cyclophotocoagulation(TCP) performed with two different probes, the G-probe(IRIDEX Medical Instruments, Mountain View, CA, USA) and the Ciliprobe(Katalyst Surgical, Chesterfield, MO, USA). TCP was performed on two human cadaver eyes from the same corpse. The vertical meridian was marked and opposite sides were treated using either the G-probe or Ciliprobe. The first eye was treated with each probe at 2000 ms/2000 mW and the second eye at 3000 ms/1500 mW. Histological examination revealed separation and loss of the non-pigmented ciliary epithelium as well as vacuolization in all sections for both probes and settings. Changes to the non-pigmented ciliary epithelium treated at 3000 ms/1500 mW were similar between the two probes. A slightly more complete separation of the non-pigmented epithelium was noted on the Ciliprobe treated sections as compared to the G-probe treated sections in the eye treated at 2000 ms/2000 mW. Therefore, in human cadaver eyes, both the G-probe and Ciliprobe produced separation, vacuolization, and loss of the non-pigmented ciliary epithelium at two different, clinically utilized settings.
