As the largest multifunctional and dynamic organelle in fungi,vacuoles are associated with different organelles through membrane contact sites and participate in various cellular processes.Vacuole and mitochondria pat...As the largest multifunctional and dynamic organelle in fungi,vacuoles are associated with different organelles through membrane contact sites and participate in various cellular processes.Vacuole and mitochondria patch(vCLAMP),the membrane contact site that tethers vacuoles and mitochondria,is indispensable for reciprocal interplay between these two organelles.The impairment of vacuoles and mitochondria significantly suppressed FB_1 production in Fusarium verticillioides.However,the understanding of how vCLAMP complex regulates fumonisin biosynthesis remained unknown.Herein,the biological functions of vCLAMP component Vam6 were investigated in F. verticillioides.Our results showed that FvVam6 deletion mutant ΔFvVam6 exhibited palpable defects in fungal development,stresses responses and pathogenicity.In addition,abnormal vacuolar morphology and significantly reduced FB_1,production were observed in ΔFvVam6.Furthermore,we demonstrated that two vacuolar sorting protein 39(Vps39) domains and clathrin domain were critical for the biological functions of FvVam6,while clathrin and Vps39-2 domains played dominant roles in the regulation of virulence and FB_1 production.Taken together,our results advanced our understanding of vCLAMP in fumonisin biosynthesis in plant pathogenic fungus F. verticillioides.展开更多
基金supported by the National Natural Science Foundation of China (32202354 to Zehua Zhou)the Scientific Research Fund of Hunan Provincial Education Department, China (21B0222 to Zehua Zhou)。
文摘As the largest multifunctional and dynamic organelle in fungi,vacuoles are associated with different organelles through membrane contact sites and participate in various cellular processes.Vacuole and mitochondria patch(vCLAMP),the membrane contact site that tethers vacuoles and mitochondria,is indispensable for reciprocal interplay between these two organelles.The impairment of vacuoles and mitochondria significantly suppressed FB_1 production in Fusarium verticillioides.However,the understanding of how vCLAMP complex regulates fumonisin biosynthesis remained unknown.Herein,the biological functions of vCLAMP component Vam6 were investigated in F. verticillioides.Our results showed that FvVam6 deletion mutant ΔFvVam6 exhibited palpable defects in fungal development,stresses responses and pathogenicity.In addition,abnormal vacuolar morphology and significantly reduced FB_1,production were observed in ΔFvVam6.Furthermore,we demonstrated that two vacuolar sorting protein 39(Vps39) domains and clathrin domain were critical for the biological functions of FvVam6,while clathrin and Vps39-2 domains played dominant roles in the regulation of virulence and FB_1 production.Taken together,our results advanced our understanding of vCLAMP in fumonisin biosynthesis in plant pathogenic fungus F. verticillioides.
