OBJECTIVE:To investigate the protective efficacy of Bunao Fuyuan decoction(BNFY)on cerebral Ischemia/reperfusion(I/R)injury.METHODS:The mouse PC12 cells were chosen,and the oxidative-glucose deprivation/re-oxygenation...OBJECTIVE:To investigate the protective efficacy of Bunao Fuyuan decoction(BNFY)on cerebral Ischemia/reperfusion(I/R)injury.METHODS:The mouse PC12 cells were chosen,and the oxidative-glucose deprivation/re-oxygenation(OGD/R)injury model were established to simulate cerebral I/R injury.Atorvastatin was selected as a positive drug,and a gradient dose of BNFY was given for 6,12 and 24 h.3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay were used to detect cell viability at each time point.Cell apoptosis was measured by terminal deoxynucleotidyl transferase-mediated d UTP-botin nick end labeling(TUNEL)staining.enzyme linked immunosorbent assay was used to detect the expression of tumor necrosis factor(TNF)-α,interleukin(IL)-6,IL-1βand platelet activating factor(PAF).Western blot assay were performed to detect the expression of key regulators[toll-like receptor 4(TLR4),nuclear factor kappa-B(NF-κB),p-p38 mito-gen-activated protein kinase(MAPK)and p-Akt(also known as protein kinase B,PKB)]of cell survival and inflammatory response.RESULTS:The results of MTT assay and TUNEL staining assay revealed that BNFY significantly increased cell viability and inhibited cell apoptosis of PC12 cells following OGD/R,respectively.Furthermore,the expression of TNF-α,1 L-6,1 L-1βand PAF were decreased after BNFY treatment.And the results of Western blot assay showed that BNFY downregulated TLR4,NF-κB,p-p38 MAPK expression and upregulated p-Akt expression.CONCLUSION:Our findings suggest that BNFY may play a role in protecting OGD/R injured PC12 cells through inhibiting the inflammatory response and cell apoptosis.展开更多
目的观察益气升阳复元汤对轻度子宫脱垂磁刺激治疗患者临床疗效及盆底肌力的影响。方法将该院2020年2月-2022年6月收治的260例轻度子宫脱垂患者根据简单随机法分为两组。磁刺激组给予磁刺激治疗,联合组在磁刺激治疗基础上给予益气升阳...目的观察益气升阳复元汤对轻度子宫脱垂磁刺激治疗患者临床疗效及盆底肌力的影响。方法将该院2020年2月-2022年6月收治的260例轻度子宫脱垂患者根据简单随机法分为两组。磁刺激组给予磁刺激治疗,联合组在磁刺激治疗基础上给予益气升阳复元汤治疗。比较两组总有效率及1、2类肌纤维的肌力强度≥3级比例,评估两组中医症状评分、生活质量量表(short from health survey,SF-36)评分、子宫脱垂C点距离的差异,统计两组12个月内复发情况。结果联合组总有效率为93.85%(122/130)高于磁刺激组的85.38%(111/130),差异有统计学意义(P<0.05)。与该组治疗前比,两组治疗后子宫下移、劳则加剧、小腹坠胀、腰腹酸痛、面色少华、神疲乏力、小便频数、带下增多评分均降低,联合组较磁刺激组更低(P<0.05)。与该组治疗前比,两组治疗后SF-36评分均升高,联合组除躯体疼痛、总体健康、活力3项评分外其他评分均较磁刺激组更低(P<0.05)。以C点为参照点,与该组治疗前比,两组治疗后子宫脱垂C点距离均升高,联合组较磁刺激组更高(P<0.05)。与该组治疗前比,两组治疗后1、2类肌纤维的肌力强度≥3级比例均升高,联合组较磁刺激组更高(P<0.05)。联合组复发率为6.92%(111/130)低于磁刺激组的18.46%(122/130),差异有统计学意义(P<0.05)。结论益气升阳复元汤可改善轻度子宫脱垂磁刺激治疗患者的子宫脱垂程度,减轻症状,提高盆底肌力,降低复发率。展开更多
文摘OBJECTIVE:To investigate the protective efficacy of Bunao Fuyuan decoction(BNFY)on cerebral Ischemia/reperfusion(I/R)injury.METHODS:The mouse PC12 cells were chosen,and the oxidative-glucose deprivation/re-oxygenation(OGD/R)injury model were established to simulate cerebral I/R injury.Atorvastatin was selected as a positive drug,and a gradient dose of BNFY was given for 6,12 and 24 h.3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay were used to detect cell viability at each time point.Cell apoptosis was measured by terminal deoxynucleotidyl transferase-mediated d UTP-botin nick end labeling(TUNEL)staining.enzyme linked immunosorbent assay was used to detect the expression of tumor necrosis factor(TNF)-α,interleukin(IL)-6,IL-1βand platelet activating factor(PAF).Western blot assay were performed to detect the expression of key regulators[toll-like receptor 4(TLR4),nuclear factor kappa-B(NF-κB),p-p38 mito-gen-activated protein kinase(MAPK)and p-Akt(also known as protein kinase B,PKB)]of cell survival and inflammatory response.RESULTS:The results of MTT assay and TUNEL staining assay revealed that BNFY significantly increased cell viability and inhibited cell apoptosis of PC12 cells following OGD/R,respectively.Furthermore,the expression of TNF-α,1 L-6,1 L-1βand PAF were decreased after BNFY treatment.And the results of Western blot assay showed that BNFY downregulated TLR4,NF-κB,p-p38 MAPK expression and upregulated p-Akt expression.CONCLUSION:Our findings suggest that BNFY may play a role in protecting OGD/R injured PC12 cells through inhibiting the inflammatory response and cell apoptosis.
文摘目的观察益气升阳复元汤对轻度子宫脱垂磁刺激治疗患者临床疗效及盆底肌力的影响。方法将该院2020年2月-2022年6月收治的260例轻度子宫脱垂患者根据简单随机法分为两组。磁刺激组给予磁刺激治疗,联合组在磁刺激治疗基础上给予益气升阳复元汤治疗。比较两组总有效率及1、2类肌纤维的肌力强度≥3级比例,评估两组中医症状评分、生活质量量表(short from health survey,SF-36)评分、子宫脱垂C点距离的差异,统计两组12个月内复发情况。结果联合组总有效率为93.85%(122/130)高于磁刺激组的85.38%(111/130),差异有统计学意义(P<0.05)。与该组治疗前比,两组治疗后子宫下移、劳则加剧、小腹坠胀、腰腹酸痛、面色少华、神疲乏力、小便频数、带下增多评分均降低,联合组较磁刺激组更低(P<0.05)。与该组治疗前比,两组治疗后SF-36评分均升高,联合组除躯体疼痛、总体健康、活力3项评分外其他评分均较磁刺激组更低(P<0.05)。以C点为参照点,与该组治疗前比,两组治疗后子宫脱垂C点距离均升高,联合组较磁刺激组更高(P<0.05)。与该组治疗前比,两组治疗后1、2类肌纤维的肌力强度≥3级比例均升高,联合组较磁刺激组更高(P<0.05)。联合组复发率为6.92%(111/130)低于磁刺激组的18.46%(122/130),差异有统计学意义(P<0.05)。结论益气升阳复元汤可改善轻度子宫脱垂磁刺激治疗患者的子宫脱垂程度,减轻症状,提高盆底肌力,降低复发率。
