Fusarium monilljorme, a fungus of established carcinogenic potential, is one of the most common fungal contaminants of maize, millet and other grains in Linxian County, China. Fusarin C, a major product of F. monilljo...Fusarium monilljorme, a fungus of established carcinogenic potential, is one of the most common fungal contaminants of maize, millet and other grains in Linxian County, China. Fusarin C, a major product of F. monilljorme grown on corn in the laboratory, is mutagenic in Salmonella tester strains and in V79 cells. Fusarin C showed several characteristics of malignant transformation including the implantation of the rat esophageal epithelial cell line (RE ?525) in nude mice. The present work demonstrated that fusarin C can induce esophageal and forestomach carcinomas in DBA mice and Wistar rats, and thus the experimental results substantiated further the carcinogenicity of fusarin C.展开更多
Fusarin C (FC) is a mutagenic and carcinogenic mycotoxin which was isolated from Fu-sarium moniliforme culture extracts. The Fusarium moniliforme is one of most prevalent fungi found on corn in Linxian, a high risk ar...Fusarin C (FC) is a mutagenic and carcinogenic mycotoxin which was isolated from Fu-sarium moniliforme culture extracts. The Fusarium moniliforme is one of most prevalent fungi found on corn in Linxian, a high risk area for esophageal cancer. This paper reports, for the first time, the malignant transformation of rat esophageal epithelial cells induced by FC. The transformed cells showed several characteristics of transformation. Colonies were formed after seeding these transformed cells either into selective medium free of epidermal growth factor (EGF) and serum, or on semi-solid agar; there was an increase in chromosome number; the expression of c-myc and v-erb-B oncogenes was enhanced in the cells; and squamous cell carcinomas arose after inoculating the cells into nude mice. The results demonstrated transforming effect of FC on rat esophageal epithelial cells, and indicate that the abnormal expression of some oncogenes could serve as a hew property of transformed cells.展开更多
Fusarium moniliforme is a predominant fungus found in fungus-contaminated food in Linxian County, China, a high risk area of esophageal. cancer. Rats fed with corn bread inoculated with Fusarium moniliforme developed ...Fusarium moniliforme is a predominant fungus found in fungus-contaminated food in Linxian County, China, a high risk area of esophageal. cancer. Rats fed with corn bread inoculated with Fusarium moniliforme developed papillomas and squamous-cell carcinomas展开更多
串珠镰刀菌可利用羟基脯氨酸、蔗糖、甘油和珍珠岩(P)等组成的 P 液体培养基合成镰刀菌素 C(Fc),其最高量为936mg/kg 有机物,比在玉米渣培养基中形成的 Fc 量较高。用 P液体培养基制备 Fc,受蔗糖浓度、胺类和培养温度及培养时间等的影...串珠镰刀菌可利用羟基脯氨酸、蔗糖、甘油和珍珠岩(P)等组成的 P 液体培养基合成镰刀菌素 C(Fc),其最高量为936mg/kg 有机物,比在玉米渣培养基中形成的 Fc 量较高。用 P液体培养基制备 Fc,受蔗糖浓度、胺类和培养温度及培养时间等的影响。实验证明,由 (?)g 羟基脯氨酸、40g 蔗糖和珍珠岩组成的 P 液体培养基,在28℃培养二周是形成 Fc 的理想条件。液体培养基中加入珍珠岩,Fc 的形成量增加500多倍。展开更多
文摘Fusarium monilljorme, a fungus of established carcinogenic potential, is one of the most common fungal contaminants of maize, millet and other grains in Linxian County, China. Fusarin C, a major product of F. monilljorme grown on corn in the laboratory, is mutagenic in Salmonella tester strains and in V79 cells. Fusarin C showed several characteristics of malignant transformation including the implantation of the rat esophageal epithelial cell line (RE ?525) in nude mice. The present work demonstrated that fusarin C can induce esophageal and forestomach carcinomas in DBA mice and Wistar rats, and thus the experimental results substantiated further the carcinogenicity of fusarin C.
文摘Fusarin C (FC) is a mutagenic and carcinogenic mycotoxin which was isolated from Fu-sarium moniliforme culture extracts. The Fusarium moniliforme is one of most prevalent fungi found on corn in Linxian, a high risk area for esophageal cancer. This paper reports, for the first time, the malignant transformation of rat esophageal epithelial cells induced by FC. The transformed cells showed several characteristics of transformation. Colonies were formed after seeding these transformed cells either into selective medium free of epidermal growth factor (EGF) and serum, or on semi-solid agar; there was an increase in chromosome number; the expression of c-myc and v-erb-B oncogenes was enhanced in the cells; and squamous cell carcinomas arose after inoculating the cells into nude mice. The results demonstrated transforming effect of FC on rat esophageal epithelial cells, and indicate that the abnormal expression of some oncogenes could serve as a hew property of transformed cells.
文摘Fusarium moniliforme is a predominant fungus found in fungus-contaminated food in Linxian County, China, a high risk area of esophageal. cancer. Rats fed with corn bread inoculated with Fusarium moniliforme developed papillomas and squamous-cell carcinomas
文摘串珠镰刀菌可利用羟基脯氨酸、蔗糖、甘油和珍珠岩(P)等组成的 P 液体培养基合成镰刀菌素 C(Fc),其最高量为936mg/kg 有机物,比在玉米渣培养基中形成的 Fc 量较高。用 P液体培养基制备 Fc,受蔗糖浓度、胺类和培养温度及培养时间等的影响。实验证明,由 (?)g 羟基脯氨酸、40g 蔗糖和珍珠岩组成的 P 液体培养基,在28℃培养二周是形成 Fc 的理想条件。液体培养基中加入珍珠岩,Fc 的形成量增加500多倍。
